The effect of silk gland sericin protein incorporation into electrospun polycaprolactone nanofibers on in vitro and in vivo characteristics

2015 ◽  
Vol 3 (5) ◽  
pp. 859-870 ◽  
Author(s):  
Linhao Li ◽  
Yuna Qian ◽  
Chongwen Lin ◽  
Haibin Li ◽  
Chao Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Gland ◽  
Engineering Applications ◽  
Nanofibrous Scaffolds ◽  
Protein Incorporation

Silk middle gland extracted sericin protein based electrospun nanofibrous scaffolds with excellent biocompatibility have been developed for tissue engineering applications.

Bioprinted Nanoparticles for Tissue Engineering Applications

2009 ◽  
Author(s):  
Kivilcim Buyukhatipoglu ◽  
Robert Chang ◽  
Wei Sun ◽  
Alisa Morss Clyne
Keyword(s):  
Tissue Engineering ◽  
Tissue Growth ◽  
Bioactive Components ◽  
Engineering Applications ◽  
Tissue Properties ◽  
Native Tissue ◽  
3D Architecture

Tissue engineering may require precise patterning of cells and bioactive components to recreate the complex, 3D architecture of native tissue. However, it is difficult to image and track cells and bioactive factors once they are incorporated into the tissue engineered construct. These bioactive factors and cells may also need to be moved during tissue growth in vitro or after implantation in vivo to achieve the desired tissue properties, or they may need to be removed entirely prior to implantation for biosafety concerns.

scholarly journals Gellan Gum Injectable Hydrogels for Cartilage Tissue Engineering Applications: In Vitro Studies and Preliminary In Vivo Evaluation

2010 ◽  
Vol 16 (1) ◽  
pp. 343-353 ◽  
Author(s):  
João T. Oliveira ◽  
Tírcia C. Santos ◽  
Luís Martins ◽  
Ricardo Picciochi ◽  
Alexandra P. Marques ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Gellan Gum ◽  
In Vitro Studies ◽  
Cartilage Tissue ◽  
Engineering Applications ◽  
In Vivo Evaluation ◽  
Injectable Hydrogels

Novel HA-PVA/NOCC bilayered scaffold for osteochondral tissue-engineering applications – Fabrication, characterization, in vitro and in vivo biocompatibility study

2013 ◽  
Vol 113 ◽  
pp. 25-29 ◽  
Author(s):  
Nurul Syuhada Ibrahim ◽  
Genasan Krishnamurithy ◽  
Hanumantha Rao Balaji Raghavendran ◽  
Subramaniam Puvaneswary ◽  
Ng Wuey Min ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Applications ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering ◽  
Biocompatibility Study

A review on 3D printing in tissue engineering applications

2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohan Prasath Mani ◽  
Madeeha Sadia ◽  
Saravana Kumar Jaganathan ◽  
Ahmad Zahran Khudzari ◽  
Eko Supriyanto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Cell Adherence ◽  
Engineering Applications ◽  
Proliferation And Differentiation ◽  
3D Printed ◽  
Printing Techniques

Abstract In tissue engineering, 3D printing is an important tool that uses biocompatible materials, cells, and supporting components to fabricate complex 3D printed constructs. This review focuses on the cytocompatibility characteristics of 3D printed constructs, made from different synthetic and natural materials. From the overview of this article, inkjet and extrusion-based 3D printing are widely used methods for fabricating 3D printed scaffolds for tissue engineering. This review highlights that scaffold prepared by both inkjet and extrusion-based 3D printing techniques showed significant impact on cell adherence, proliferation, and differentiation as evidenced by in vitro and in vivo studies. 3D printed constructs with growth factors (FGF-2, TGF-β1, or FGF-2/TGF-β1) enhance extracellular matrix (ECM), collagen I content, and high glycosaminoglycan (GAG) content for cell growth and bone formation. Similarly, the utilization of 3D printing in other tissue engineering applications cannot be belittled. In conclusion, it would be interesting to combine different 3D printing techniques to fabricate future 3D printed constructs for several tissue engineering applications.

scholarly journals In vitro and in vivo Biocompatibility of Alginate Dialdehyde/Gelatin Hydrogels with and without Nanoscaled Bioactive Glass for Bone Tissue Engineering Applications

Materials ◽  
2014 ◽  
Vol 7 (3) ◽  
pp. 1957-1974 ◽  
Author(s):  
Ulrike Rottensteiner ◽  
Bapi Sarker ◽  
Dominik Heusinger ◽  
Diana Dafinova ◽  
Subha Rath ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Engineering Applications

Non-invasive in vitro and in vivo monitoring of degradation of fluorescently labeled hyaluronan hydrogels for tissue engineering applications

2016 ◽  
Vol 30 ◽  
pp. 188-198 ◽  
Author(s):  
Yu Zhang ◽  
Filippo Rossi ◽  
Simonetta Papa ◽  
Martina Bruna Violatto ◽  
Paolo Bigini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Applications ◽  
In Vivo Monitoring ◽  
Non Invasive

scholarly journals Gold-Polymer Nanocomposites for Future Therapeutic and Tissue Engineering Applications

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 70
Author(s):  
Panangattukara Prabhakaran Praveen Kumar ◽  
Dong-Kwon Lim
Keyword(s):  
Tissue Engineering ◽  
Polymer Nanocomposites ◽  
Disease Diagnosis ◽  
The Body ◽  
Hybrid Nanocomposites ◽  
Surface Charges ◽  
Engineering Applications ◽  
Therapeutic Applications

Gold nanoparticles (AuNPs) have been extensively investigated for their use in various biomedical applications. Owing to their biocompatibility, simple surface modifications, and electrical and unique optical properties, AuNPs are considered promising nanomaterials for use in in vitro disease diagnosis, in vivo imaging, drug delivery, and tissue engineering applications. The functionality of AuNPs may be further expanded by producing hybrid nanocomposites with polymers that provide additional functions, responsiveness, and improved biocompatibility. Polymers may deliver large quantities of drugs or genes in therapeutic applications. A polymer alters the surface charges of AuNPs to improve or modulate cellular uptake efficiency and their biodistribution in the body. Furthermore, designing the functionality of nanocomposites to respond to an endo- or exogenous stimulus, such as pH, enzymes, or light, may facilitate the development of novel therapeutic applications. In this review, we focus on the recent progress in the use of AuNPs and Au-polymer nanocomposites in therapeutic applications such as drug or gene delivery, photothermal therapy, and tissue engineering.

scholarly journals Efficacy of Bacterial Nanocellulose in Hard Tissue Regeneration: A Review

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 4777
Author(s):  
Anuj Kumar ◽  
Sung-Soo Han
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cellular Response ◽  
Future Research ◽  
Hydroxyl Groups ◽  
Hard Tissue ◽  
Engineering Applications ◽  
Bacterial Nanocellulose

Bacterial nanocellulose (BNC, as exopolysaccharide) synthesized by some specific bacteria strains is a fascinating biopolymer composed of the three-dimensional pure cellulosic nanofibrous matrix without containing lignin, hemicellulose, pectin, and other impurities as in plant-based cellulose. Due to its excellent biocompatibility (in vitro and in vivo), high water-holding capacity, flexibility, high mechanical properties, and a large number of hydroxyl groups that are most similar characteristics of native tissues, BNC has shown great potential in tissue engineering applications. This review focuses on and discusses the efficacy of BNC- or BNC-based biomaterials for hard tissue regeneration. In this review, we provide brief information on the key aspects of synthesis and properties of BNC, including solubility, biodegradability, thermal stability, antimicrobial ability, toxicity, and cellular response. Further, modification approaches are discussed briefly to improve the properties of BNC or BNC-based structures. In addition, various biomaterials by using BNC (as sacrificial template or matrix) or BNC in conjugation with polymers and/or fillers are reviewed and discussed for dental and bone tissue engineering applications. Moreover, the conclusion with perspective for future research directions of using BNC for hard tissue regeneration is briefly discussed.

scholarly journals Plant-derived Cellulose Scaffolds for Bone Tissue Engineering

2020 ◽  
Author(s):  
Maxime Leblanc Latour ◽  
Maryam Tarar ◽  
Ryan J. Hickey ◽  
Charles M. Cuerrier ◽  
Isabelle Catelas ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Structural Features ◽  
Osteogenic Potential ◽  
Engineering Applications ◽  
Alizarin Red

AbstractPlant-derived cellulose biomaterials have recently been utilized in several tissue engineering applications. Naturally-derived cellulose scaffolds have been shown to be highly biocompatible in vivo, possess structural features of relevance to several tissues, as well as support mammalian cell invasion and proliferation. Recent work utilizing decellularized apple hypanthium tissue has shown that it possesses a pore size and properties similar to trabecular bone. In the present study, we examined the potential of apple-derived cellulose scaffolds for bone tissue engineering (BTE). Confocal microscopy revealed that the scaffolds had a suitable pore size for BTE applications. To analyze their in vitro mineralization potential, MC3T3-E1 pre-osteoblasts were seeded in either bare cellulose scaffolds or in composite scaffolds composed of cellulose and collagen I. Following chemically-induced differentiation, scaffolds were mechanically tested and evaluated for mineralization. The Young’s modulus of both types of scaffolds significantly increased after cell differentiation. Alkaline phosphatase and Alizarin Red staining further highlighted the osteogenic potential of the scaffolds. Histological sectioning of the constructs revealed complete invasion by the cells and mineralization throughout the entire constructs. Finally, scanning electron microscopy demonstrated the presence of mineral aggregates deposited on the scaffolds after differentiation, and energy-dispersive spectroscopy confirmed the presence of phosphate and calcium. In summary, our results indicate that plant-derived cellulose is a promising scaffold candidate for bone tissue engineering applications.

Radiation Effect on Poly(ε-Caprolactone) Nanofibrous Scaffold

2007 ◽  
Vol 119 ◽  
pp. 95-98
Author(s):  
Youn Mook Lim ◽  
Joon Pyo Jeun ◽  
Chan Hee Jung ◽  
Jae Hak Choi ◽  
Phil Hyun Kang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Radiation Effect ◽  
Average Diameter ◽  
Nanofibrous Scaffolds ◽  
Biomimetic Scaffolds ◽  
Biodegradable Poly ◽  
Γ Ray

Nano- to micro-structured biodegradable poly(ε-caprolactone) nanofibrous scaffolds (PCL NFSs) were prepared by an electrospinning. Electrospinning has recently emerged as a leading technique for generating the biomimetic scaffolds for tissue engineering applications. The average diameter of the electrospun PCL NFSs ranged from 0.5 to 2 ㎛ depending on the solvent/nonsolvent mixture. PCL NFSs were irradiated using γ-ray and their mechanical properties and biodegradability were measured. In vitro/vivo degradation studies of the scaffolds as a function of the radiation dose were performed. The scaffolds were degraded more slowly in vitro than in vivo.

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