Biomimetic synthesis of needle-like fluorescent calcium phosphate/carbon dot hybrid composites for cell labeling and copper ion detection

2016 ◽  
Vol 45 (18) ◽  
pp. 7665-7671 ◽  
Author(s):  
Shanshan Guo ◽  
Shousi Lu ◽  
Pingxiang Xu ◽  
Yi Ma ◽  
Liang Zhao ◽  
...  
Keyword(s):  
Drinking Water ◽  
Calcium Phosphate ◽  
Copper Ions ◽  
Hybrid Composites ◽  
Copper Ion ◽  
Cell Labeling ◽  
Biomimetic Synthesis ◽  
Selective Detection ◽  
Sodium Carboxymethylcellulose ◽  
Biomimetic Method

We report a biomimetic method to synthesize needle-like calcium phosphate (CaP) using carbon dots (CDs) and sodium carboxymethylcellulose as dual templates. The CaP/CDs were capable of cell labeling and selective detection of copper ions in drinking water.

Biomimetic Synthesis of the Nanocomposite of Phosphorylatd Chitosan and Hydroxyapatite and its Bioactivity In Vitro

2007 ◽  
Vol 330-332 ◽  
pp. 721-724
Author(s):  
Quan Li Li ◽  
Nan Huang ◽  
Zhi Qing Chen ◽  
Xu Yan Tang
Keyword(s):  
Calcium Phosphate ◽  
Bone Repair ◽  
Biomimetic Synthesis ◽  
The Novel ◽  
Culture In Vitro ◽  
Axis Direction ◽  
Osteoblast Culture ◽  
Good Biocompatibility ◽  
Biomimetic Method

Phosphorylated chitosan (PCS) was used as the template to control hydroxyapatite(HAp) growth, a novel nanocomposite composed of PCS and HAp was synthesized by biomimetic method. Calcium phosphate (Ca 2+= 60mM, Ca/P = 1.67) solution in HCl was added dropwise into PCS solution in NaOH. The precipitate was lyophilized to obtain the composite. The biocompatibility of the PCS-HAp nanocomopite was evaluated by osteoblast culture in vitro. The results showed that low crystallized HAp nanocrystals was formed on the PCS fibers and its crystallographic c-axis were aligned preferentially parallel to the long axis direction of PCS; the composite have good biocompatibility in vitro. It is expected that the novel composite to be a potential material for bone repair.

scholarly journals The Tribond Bridged Bipyridine Complex-based In-fiber Interferometric Sensor design for Cu2+ Detection

2020 ◽  
Vol 213 ◽  
pp. 02019
Author(s):  
Jiahui Huang ◽  
Lijun Li ◽  
Yumeng Lv ◽  
Min Li
Keyword(s):  
Drinking Water ◽  
Copper Ions ◽  
Copper Ion ◽  
Liquid Environment ◽  
High Affinity ◽  
Allowable Level ◽  
Core Fiber ◽  
Interferometric Sensor

We report a real-time, lightweight sensor based on in-fiber MZ interferometer coating bipyridine complex L[4-methyl-2, 6-bis(pyridine-3-ylethynyl)aniline](L-ligand) in situ determination of free copper ion at micromolar levels. The sensor is made by fusing a section of thin-core fiber(TCF) in the middle of singlemode fiber(SMF). The L-ligand material is attached to the sensing surface of thin-core fiber to bind free copper ions in liquid environment with high affinity and selectivity. Investigation was carried out that the performance of the sensor is significantly improved with different length. The sensitivity of Cu2+ detection can reach 0.0573 nm/μM (nanometer per micromolar) in linear range of 0μM~25μM and the detection limit is 0.8726μM. This is significantly lower than the allowable level of drinking water.

Biomimetic Synthesis of PEC-HA Composite Analogous to Bone

2007 ◽  
Vol 336-338 ◽  
pp. 1699-1702 ◽  
Author(s):  
Quan Li Li ◽  
Zhi Qing Chen ◽  
Brian W. Darvell ◽  
Quan Zeng ◽  
Gang Li ◽  
...  
Keyword(s):  
Composite Material ◽  
Crystal Growth ◽  
Controlled Release ◽  
Calcium Phosphate ◽  
Polyelectrolyte Complex ◽  
Biomimetic Synthesis ◽  
Hierarchical Porous Structure ◽  
Phosphate Ions ◽  
Hierarchical Porous ◽  
Biomimetic Method

A polyelectrolyte complex (PEC) composed of chitosan (CS) and phosphorylated chitosan (PCS) was used to encapsulate a calcium phosphate by a biomimetic method. An acidic CS (polycation) solution containing calcium and phosphate ions (Ca2+: 6mM, Ca/P = 1.67) was added into PCS (polyanion) solution leading to the formation of a polyelectrolyte complex (PEC) with nanoscopic carbonate-containing, low-crystallinity hydroxyapatite (HA) distributed evenly in the fibrils of the PEC by controlled crystal growth. The resulting composite material, PEC-HA, has a complicated, hierarchical porous structure that is expected to have high bio-compatibity and that may be of use as a carrier for controlled-release therapetic agents.

scholarly journals Visual Quantitation of Copper Ions Based on a Microfluidic Particle Dam Reflecting the Cu(II)-Catalyzed Oxidative Damage of DNA

Biosensors ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 487
Author(s):  
Chenyu Cui ◽  
Ting-Hsuan Chen
Keyword(s):  
Drinking Water ◽  
Limit Of Detection ◽  
Copper Ions ◽  
Tap Water ◽  
Copper Ion ◽  
Daily Basis ◽  
World Health ◽  
Water Safety ◽  
Magnetic Separator ◽  
Health Organization

Due to the use of copper water pipes and the discharge of industrial wastewater, contamination of copper ions in drinking water has become a severe hazard globally. To routinely check water safety on a daily basis, easy-to-use platforms for quantitative analysis of trace amounts of copper ions (Cu2+) in drinking water is needed. Here, we report microfluidic particle accumulation integrated with a Cu(II)-catalyzed Fenton reaction for visual and quantitative copper ion detection. Microparticles (MMPs) and polystyrene microparticles (PMPs) are connected via a single strand DNA, MB155. However, when Cu2+ is present, MB155 is cleaved by hydroxyl free radicals (•OH) produced from Cu2+/hydrogen peroxide (H2O2) Fenton reactions, causing an increased amount of free PMPs. To visually count them, the particle solution is loaded onto a microfluidic chip where free MMPs and MMPs–MB155–PMPs can be collected by the magnetic separator, while the free PMPs continue flowing until being accumulated at the particle dam. The results showed a good linear relationship between the trapping length of PMP accumulation and the Cu2+ concentration from 0 to 300 nM. A limit of detection (LOD) of 70.1 nM was achieved, which is approximately 449 times lower than the 2 × 103 μg·L−1 (~31.5 μM) required by the World Health Organization (WHO). Moreover, the results showed high selectivity and good tolerance to pH and hardness, indicating compatibility for detection in tap water, suggesting a potential platform for the routine monitoring of copper contamination in drinking water.

A Bright Nitrogen-doped-Carbon-Dots based Fluorescent Biosensor for Selective Detection of Copper Ions

2021 ◽  
Vol 5 (1) ◽  
pp. 84-92
Author(s):  
Shuting Chen ◽  
Chaoqun Chen ◽  
Jian Wang ◽  
Fang Luo ◽  
Longhua Guo ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Copper Ions ◽  
Selective Detection ◽  
Nitrogen Doped ◽  
Fluorescent Biosensor ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

scholarly journals An Investigation on Design and Characterization of a Highly Selective LED Optical Sensor for Copper Ions in Aqueous Solutions

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1099
Author(s):  
Sheng-Chun Hung ◽  
Chih-Cheng Lu ◽  
Yu-Ting Wu
Keyword(s):  
Aqueous Solutions ◽  
Metal Ions ◽  
Copper Ions ◽  
Incident Light ◽  
Copper Ion ◽  
Intensity Change ◽  
Optical Absorbance ◽  
Sensing System ◽  
Optic Sensor ◽  
Cu Ions

The optical characteristics of copper ion detection, such as the photometric absorbance of specific wavelengths, exhibit significant intensity change upon incident light into the aqueous solutions with different concentrations of metal ions due to the electron transition in the orbit. In this study, we developed a low-cost, small-size and fast-response photoelectric sensing prototype as an optic sensor for copper (Cu) ions detection by utilizing the principle of optical absorption. We quantified the change of optical absorbance from infra-red (IR) light emitting diodes (LEDs) upon different concentrations of copper ions and the transmitted optical signals were transferred to the corresponding output voltage through a phototransistor and circuit integrated in the photoelectric sensing system. The optic sensor for copper (Cu) ions demonstrated not only excellent specificity with other metal ions such as cadmium (Cd), nickel (Ni), iron (Fe) and chloride (Cl) ions in the same aqueous solution but also satisfactory linearity and reproducibility. The sensitivity of the preliminary sensing system for copper ions was 29 mV/ppm from 0 to 1000 ppm. In addition, significant ion-selective characteristics and anti-interference capability were also observed in the experiments by the proposed approach.

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

A REVERSIBLE OPTICAL SENSOR BASED ON CHITOSAN FILM FOR THE SELECTIVE DETECTION OF COPPER IONS

2012 ◽  
Vol 24 (05) ◽  
pp. 453-459 ◽  
Author(s):  
Shenhsiung Lin ◽  
Chia-Chen Chang ◽  
Chii-Wann Lin
Keyword(s):  
Functional Groups ◽  
Inductively Coupled Plasma ◽  
Copper Ions ◽  
Three Dimensional ◽  
Copper Ion ◽  
Chitosan Film ◽  
Detection Limits ◽  
Dimensional Structure ◽  
Optical Detectors ◽  
Inductively Coupled

Heavy metals greatly influence animal physiology, even at small doses. Among these metals, the copper ion is of great concern due to its effects on humans and wide applications in industry. Compared to atomic absorption spectroscopy and inductively coupled plasma-mass spectrometry, which destroy the samples that are analyzed, optical techniques do not decompose the analyte and have become a popular field of recent research. In this paper, we combined a novel optical detector that did not require sample-labeling, called surface plasmon resonance (SPR), with chitosan to detect copper ions by modifying the functional groups of chitosan through pH modification. Compared to other optical detectors, the SPR system was relatively fast and involved fewer experimental confounding factors. The three-dimensional structure of chitosan was used to obtain lower detection limits. Moreover, modification of the chitosan functional groups resulted in efficient regeneration by controlling the pH. A detection limit of 0.1 μM was obtained (linear range: 0.5–10 μM, R2 = 0.976), and the specificity was certified by comparing the copper ion with six other ions. Additionally, we successfully regenerated the SPR chips by modifying the functional groups. In conclusion, the chitosan–SPR system detected copper ions with improved detection limits using a quick and simple regeneration method.

scholarly journals Harnessing of Newly Tailored Poly (Acrylonitrile)-Starch Nanoparticle Graft Copolymer for Copper Ion Removal via Oximation Reaction

2021 ◽  
Author(s):  
khaled Mostafa ◽  
H. Ameen ◽  
A. Ebessy ◽  
A. El-Sanabary
Keyword(s):  
Experimental Data ◽  
Adsorption Capacity ◽  
Graft Copolymer ◽  
Adsorption Kinetics ◽  
Copper Ions ◽  
Copper Ion ◽  
Second Order ◽  
First Order ◽  
Pseudo Second Order ◽  
Pseudo First Order

Abstract Our recently tailored and fully characterized poly (AN)-starch nanoparticle graft copolymer having 60.1 G.Y. % was used as a starting substrate for copper ions removal from waste water effluent after chemical modification with hydroxyl amine via oximation reaction. This was done to change the abundant nitrile groups in the above copolymer into amidoxime one and the resultant poly (amidoxime) resin was used as adsorbent for copper ions. The resin was characterized qualitatively via rapid vanadium ion test and instrumentally by FT-IR spectra and SEM morphological analysis to confirm the presence of amidoxime groups. The adsorption capacity of the resin was done using the batch technique, whereas the residual copper ions content in the filtrate before and after adsorption was measured using atomic adsorption spectrometry. It was found that the maximum adsorption capacity of poly (amidoxime) resin was 115.2 mg/g at pH 7, 400ppm copper ions concentration and 0.25 g adsorbent at room temperature. The adsorption, kinetics and isothermal study of the process is scrutinized using different variables, such as pH, contact time, copper ion concentration and adsorbent dosage. Different kinetics models comprising the pseudo-first-order and pseudo-second-order have been applied to the experimental data to envisage the adsorption kinetics. It was found from kinetic study that pseudo-second-order rate equation was better than pseudo-first-order supporting the formation of chemisorption process. While, in case of isothermal study, the examination of calculated correlation coefficient (R2) values showed that the Langmuir model provide the best fit to experimental data than Freundlich one.

scholarly journals Natural Batch Adsorption Operation to Remove Dissolved Copper (II) by Carbon Charcoal Rambutan

2021 ◽  
Vol 2129 (1) ◽  
pp. 012010
Author(s):  
B Haryanto ◽  
S E Saragih ◽  
R Tambun ◽  
H Harahap ◽  
K Manik ◽  
...  
Keyword(s):  
Metal Ions ◽  
Copper Ions ◽  
Copper Ion ◽  
Mesh Size ◽  
Total Weight ◽  
Batch Adsorption ◽  
Adsorbent Surface ◽  
Instrument Analysis

Abstract Carbon charcoal was made from rambutan rods and used as an adsorbent. A gram 70/100 mesh size of adsorbent was then used to adsorb 100 ml of copper ion solution with a 70 ppm concentration. In this investigation, the batch procedure was used without shaking (naturally). The charcoal carbon rambutan ability to remove the copper ion was measured by AAS. The percentage result was 48,135% or about 33,694 ppm. SEM and EDX instrument analysis have applied to confirm the presence of copper ions on the adsorbent surface. The copper ion was found at a concentration of 0.09 percent of the total weight. The carbon charcoal adsorbent in rambutan rods has the ability to purify the water contaminated by metal ions.

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