Increased intramuscular fat induced by reduced dietary protein in finishing pigs: effects on the longissimus lumborum muscle proteome

2016 ◽  
Vol 12 (8) ◽  
pp. 2447-2457 ◽  
Author(s):  
V. M. R. Pires ◽  
M. S. Madeira ◽  
A. A. Dowle ◽  
J. Thomas ◽  
A. M. Almeida ◽  
...  
Keyword(s):  
Dietary Protein ◽  
Fibre Type ◽  
Intramuscular Fat ◽  
Finishing Pigs ◽  
Muscle Proteome ◽  
Longissimus Lumborum ◽  
Protein Diets

Reduced protein diets affects the amounts of proteins related to fibre type and structure and energy.

scholarly journals Addition of arginine and leucine to low or normal protein diets: performance, carcass characteristics and intramuscular fat of finishing pigs

2016 ◽  
Vol 14 (4) ◽  
pp. e0605 ◽  
Author(s):  
Núria Tous ◽  
Rosil Lizardo ◽  
Borja Vilà ◽  
Marina Gispert ◽  
Maria Font-i-Furnols ◽  
...  
Keyword(s):  
Average Daily Gain ◽  
Live Weight ◽  
Intramuscular Fat ◽  
Finishing Pigs ◽  
Low Protein ◽  
Dietary Crude Protein ◽  
Initial Hypothesis ◽  
Protein Diets ◽  
Daily Gain ◽  
Imf Content

The effect of dietary crude protein (CP) reduction, supplementation with arginine or leucine on intramuscular fat (IMF) content was evaluated in (Landrace × Duroc) × Pietrain pigs. One-hundred and eight barrows (67 ± 4 kg) were assigned to six diets (n=6 pens of 3 pigs each): four normal CP diets containing 16% CP from 60 to 90 kg and 13% CP from 90 to 115 kg live weight (normal protein; normal protein high Arg, normal protein high Leu or normal protein high Arg and Leu) and two low CP diets containing 14% CP from 60 to 90 kg and 11.8% CP from 90 to 115 kg live weight (with or without supplementation of both amino acids). The high Leu and Arg diets were supplemented to obtain ratios of standard ileal digestible Leu/Lys and Arg/Lys of 4 and 2, respectively. While feed to gain ratio tended to increase (p<0.05), final weight (p<0.01), average daily feed intake (ADFI) (p<0.05) and average daily gain (ADG) (p<0.01) were reduced in animals fed low-protein diets supplemented with Arg and Leu compared to the ones fed low-protein diet unsupplemented. Marbling and IMF content in loin were reduced when Arg was supplemented (p<0.05) in normal protein diets. Supplementing these diets with Arg also reduced belly weight (p<0.01) and increased lean meat percentage (p<0.05). Contrary to the initial hypothesis, reduction of CP or dietary supplementation with Leu had no effect on IMF content and supplementation with Arg reduced it.

scholarly journals Consumer Perceptions of Meat Redness Were Strongly Influenced by Storage and Display Times

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 540
Author(s):  
Maddison T. Corlett ◽  
David W. Pethick ◽  
Khama R. Kelman ◽  
Robin H. Jacob ◽  
Graham E. Gardner
Keyword(s):  
Correlation Coefficient ◽  
Storage Period ◽  
Intramuscular Fat ◽  
Consumer Perceptions ◽  
Display Time ◽  
Eye Muscle ◽  
Longissimus Lumborum ◽  
Retail Display ◽  
Lamb Growth ◽  
Long Storage

Lamb (n = 79) meat colour was scored by 879 untrained consumers using a scale of 0 (brown) to 100 (red). This consumer colour score (CCS) was obtained on m. longissimus lumborum (loin) and m. semimembranosus (topside), stored for short (5–7 days), medium (33–35 days), and long periods (110–112 days) and a retail display time of up to 4 days. Consumers perceived topside to be less red initially and changed from red to brown more rapidly when stored for the long-storage period (p < 0.01). Whereas, the initial CCS of loin samples were similar across the storage periods (p > 0.05). CCS and the instrument measure oxy/met (reflectance of light at wavelengths 630 nm and 580 nm) had a low correlation coefficient of 0.33 (p < 0.01). The propensity for lamb growth and leanness indicated by sire breeding values for lamb weight, eye muscle depth, eye muscle fat depth, and loin intramuscular fat had varied and inconsistent effects on CCS. Therefore, even the selection on CCS.

scholarly journals Meat quality and fatty acid profile of the musculus longissimus lumborum in Czech Fleckvieh, Charolais and Charolais × Czech Fleckvieh bulls fed different types of silages

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 479-487 ◽  
Author(s):  
L. Bartoň ◽  
D. Bureš ◽  
V. Kudrna
Keyword(s):  
Fatty Acid ◽  
Meat Quality ◽  
Fatty Acid Profile ◽  
Dry Matter ◽  
Intramuscular Fat ◽  
Quality Parameters ◽  
Dietary Energy ◽  
Maize Silage ◽  
Different Types ◽  
Longissimus Lumborum

The effects of breed and diet containing different types of silages on meat quality parameters and fatty acid profile of m. longissimus lumborum (MLL) were evaluated in a total of 30 Czech Fleckvieh (CF), Charolais (CH) and Charolais &times; Czech Fleckvieh (CH &times; CF) bulls. The animals were fed two mixed diets: MS (based on maize silage) and LCS (based on legume-cereal mixture silage and lucerne silages) with different concentrations of dietary energy and fatty acids. The MLL from CH bulls had the lowest content of dry matter (P &lt; 0.01), less protein (P &lt; 0.01) and lighter meat (P &lt; 0.01) compared to the CF. The extensive LCS diet reduced dry matter (P &lt; 0.01) and intramuscular fat (P &lt; 0.01) and increased the content of hydroxyproline (P &lt; 0.05). The CH bulls exhibited higher PUFA n-3 (P &lt; 0.05) and lower MUFA (P &lt; 0.05) compared to the CF, with the CH &times; CF being intermediate. The LCS diet enhanced the proportions of PUFA (P &lt; 0.05) and PUFA n-3 (P &lt; 0.001) and reduced MUFA (P &lt; 0.001). In conclusion, both breed and diet affected the meat quality and fatty acid profile of the intramuscular fat of the bulls. The replacement of maize silage with the legume-cereal mixture and lucerne silages in the diet reduced the concentration of intramuscular fat and improved its fatty acid profile from the human nutrition perspective.

Dietary protein paradox: decrease of amino acid availability induced by high-protein diets

1993 ◽  
Vol 264 (6) ◽  
pp. G1057-G1065 ◽  
Author(s):  
C. Moundras ◽  
C. Remesy ◽  
C. Demigne
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dietary Protein ◽  
High Protein ◽  
Glutamine Metabolism ◽  
Metabolic Adaptation ◽  
Casein Diet ◽  
Serine Dehydratase ◽  
High Protein Diets ◽  
Protein Diets

The aim of the present study was to evaluate the effect of changes in dietary protein level on overall availability of amino acids for tissues. For this purpose, rats were adapted to diets containing various concentrations of casein (7.5, 15, 30, and 60%) and were sampled either during the postprandial or postabsorptive period. In rats fed the protein-deficient diet, glucogenic amino acids (except threonine) tended to accumulate in plasma, liver, and muscles. In rats fed high-protein diets, the hepatic balance of glucogenic amino acids was markedly enhanced and their liver concentrations were consistently depressed. This response was the result of a marked induction of amino acid catabolism (a 45-fold increase of liver threonine-serine dehydratase activity was observed with the 60% casein diet). The muscle concentrations of threonine, serine, and glycine underwent changes parallel to plasma and liver concentrations, and a significant reduction of glutamine was observed. During the postabsorptive period, adaptation to high-protein diets resulted in a sustained catabolism of most glucogenic amino acids, which accentuated the drop in their concentrations (especially threonine) in all the compartments studied. The time course of metabolic adaptation from a 60 to a 15% casein diet has also been investigated. Adaptation of alanine and glutamine metabolism was rapid, whereas that of threonine, serine, and glycine was delayed and required 7-11 days. This was paralleled by a relatively slow decay of liver threonine-serine dehydratase (T-SDH) activity in contrast to the rapid adaptation of pyruvate kinase activity after refeeding a high-carbohydrate diet.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Severe protein deficiency induces hepatic expression and systemic level of FGF21 but inhibits its hypothalamic expression in growing rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joanna Moro ◽  
Catherine Chaumontet ◽  
Patrick C. Even ◽  
Anne Blais ◽  
Julien Piedcoq ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Energy Expenditure ◽  
Energy Intake ◽  
Dietary Protein ◽  
Protein Deficiency ◽  
Protein Diet ◽  
Growing Rats ◽  
Low Protein ◽  
Protein Diets

AbstractTo study, in young growing rats, the consequences of different levels of dietary protein deficiency on food intake, body weight, body composition, and energy balance and to assess the role of FGF21 in the adaptation to a low protein diet. Thirty-six weanling rats were fed diets containing 3%, 5%, 8%, 12%, 15% and 20% protein for three weeks. Body weight, food intake, energy expenditure and metabolic parameters were followed throughout this period. The very low-protein diets (3% and 5%) induced a large decrease in body weight gain and an increase in energy intake relative to body mass. No gain in fat mass was observed because energy expenditure increased in proportion to energy intake. As expected, Fgf21 expression in the liver and plasma FGF21 increased with low-protein diets, but Fgf21 expression in the hypothalamus decreased. Under low protein diets (3% and 5%), the increase in liver Fgf21 and the decrease of Fgf21 in the hypothalamus induced an increase in energy expenditure and the decrease in the satiety signal responsible for hyperphagia. Our results highlight that when dietary protein decreases below 8%, the liver detects the low protein diet and responds by activating synthesis and secretion of FGF21 in order to activate an endocrine signal that induces metabolic adaptation. The hypothalamus, in comparison, responds to protein deficiency when dietary protein decreases below 5%.

EFFECTS OF GRADED DIETARY PROTEIN LEVELS ON UREA RECYCLING IN THE PIG

1982 ◽  
Vol 62 (4) ◽  
pp. 1193-1197 ◽  
Author(s):  
P. A. THACKER ◽  
J. P. BOWLAND ◽  
L. P. MILLIGAN ◽  
E. WELTZIEN
Keyword(s):  
Dietary Protein ◽  
Protein Diet ◽  
Nitrogen Excretion ◽  
Entry Rate ◽  
Protein Nitrogen ◽  
Protein Levels ◽  
Low Protein ◽  
Key Words Pig ◽  
Urea Recycling ◽  
Protein Diets

The kinetics of urea recycling were determined in six female crossbred pigs utilizing a radioisotope dilution technique. The experimental animals were fed three times daily 500 g of a corn-soybean meal diet formulated to contain 8.4, 15.8 or 24.7% crude protein. Nitrogen digestibility, urinary nitrogen excretion, total nitrogen excretion and retained nitrogen were highest on the 24.7% protein diet and decreased with decreasing dietary protein. Urea pool size, entry rate and excretion rate were also highest on the 24.7% protein diet and decreased with decreasing protein intake. Expressed as a percentage of the total entry rate, a significantly higher percentage of urea was recycled in pigs fed the low protein diets compared with those fed a higher protein diet. Key words: Pig, urea, recycling, kinetics, protein

scholarly journals Histochemical muscle fibre characteristics of German Heath lamb meat

2015 ◽  
Vol 84 (3) ◽  
pp. 297-303
Author(s):  
Milena Fantová ◽  
Martin Ptáček ◽  
Klára Michnová ◽  
Lenka Nohejlová ◽  
Jaromír Ducháček
Keyword(s):  
Fibre Type ◽  
Quadriceps Femoris ◽  
Cross Sectional Area ◽  
Meat Industry ◽  
Sectional Area ◽  
Cross Sectional ◽  
Type Distribution ◽  
Fibre Type Distribution ◽  
Longissimus Lumborum ◽  
German Origin

Histochemical characteristics of muscle were evaluated in 12 German Heath lambs (6 males and 6 females). The lambs were slaughtered at the age of 150 days and samples of musculus longissimus lumborum et thoracis and quadriceps femoris were collected and measurements of fibre cross sectional area (µm2), diameter (µm2), and perimeter (µm2) were performed. Subsequently the fibre type distribution (%) was calculated. The dataset was evaluated using SAS 9.3. Significantly higher (P < 0.05) cross sectional area, diameter, and perimeter attributes were observed in females compared to males. Significant differences (P < 0.05 to 0.01) were observed in fibre type distribution between musculus longissimus lumborum et thoracis and quadriceps femoris muscle. The presented study describes evaluation of morphological formation of skeletal muscle tissue performed on a sheep breed of German origin. The results may be useful for meat scientists or meat industry due to close connection of these characteristics to meat quality.

scholarly journals Impact of Fermentable Protein, by Feeding High Protein Diets, on Microbial Composition, Microbial Catabolic Activity, Gut Health and beyond in Pigs

2020 ◽  
Vol 8 (11) ◽  
pp. 1735
Author(s):  
Hanlu Zhang ◽  
Nikkie van der Wielen ◽  
Bart van der Hee ◽  
Junjun Wang ◽  
Wouter Hendriks ◽  
...  
Keyword(s):  
Microbial Community ◽  
Dietary Protein ◽  
Average Daily Gain ◽  
High Protein ◽  
Intestinal Morphology ◽  
Microbial Composition ◽  
Protein Levels ◽  
Catabolic Activity ◽  
High Protein Diets ◽  
Protein Diets

In pigs, high protein diets have been related to post-weaning diarrhoea, which may be due to the production of protein fermentation metabolites that were shown to have harmful effects on the intestinal epithelium in vitro. In this review, we discussed in vivo effects of protein fermentation on the microbial composition and their protein catabolic activity as well as gut and overall health. The reviewed studies applied different dietary protein levels, which was assumed to result in contrasting fermentable protein levels. A general shift to N-utilisation microbial community including potential pathogens was observed, although microbial richness and diversity were not altered in the majority of the studies. Increasing dietary protein levels resulted in higher protein catabolic activity as evidenced by increased concentration of several protein fermentation metabolites like biogenic amines in the digesta of pigs. Moreover, changes in intestinal morphology, permeability and pro-inflammatory cytokine concentrations were observed and diarrhoea incidence was increased. Nevertheless, higher body weight and average daily gain were observed upon increasing dietary protein level. In conclusion, increasing dietary protein resulted in higher proteolytic fermentation, altered microbial community and intestinal physiology. Supplementing diets with fermentable carbohydrates could be a promising strategy to counteract these effects and should be further investigated.

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