Hypoglycemic effects of phenolic compound-rich aqueous extract from water dropwort (Oenanthe javanica DC.) on streptozotocin-induced diabetic mice

2020 ◽  
Vol 44 (14) ◽  
pp. 5190-5200
Author(s):  
Shudong He ◽  
Mingming Tang ◽  
Zuoyong Zhang ◽  
Haiyan Liu ◽  
Mingfeng Luo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Aqueous Extract ◽  
Akt Pathway ◽  
Diabetic Mice ◽  
Glut4 Translocation ◽  
Hypoglycemic Action ◽  
Oenanthe Javanica ◽  
Water Dropwort

Phenolic compounds in water dropwort aqueous extract were identified, and the IRS-2/PI3K-AKT pathway and GLUT4 translocation were regulated for hypoglycemic action.

scholarly journals Effects of the Aqueous Extract of Cnestis ferruginea Leaves on the Male Reproductive System in Alloxan-induced Diabetic Mice

2021 ◽  
pp. 23-33
Author(s):  
Kouassi Emile Bégbin ◽  
N’Guessan Ernest Zougrou ◽  
Séverin Koffi ◽  
Edwige Alida Odoh ◽  
Koffi Kouakou
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Reproductive System ◽  
Histological Study ◽  
Male Reproductive System ◽  
Seminiferous Tubules ◽  
Diabetic Mice ◽  
Sperm Density

Diabetes mellitus is the most common endocrine disease and one of the most common chronic disorders. Diabetes mellitus has been associated with impaired reproductive health, mainly in men. The present study aimed to evaluate effects of the aqueous extract of Cnestis ferruginea leaves on the male reproductive system in alloxan-induced diabetic mice. The determination of phenolic compounds content in the aqueous extract was performed by conventional methods. Diabetes was induced in adult male mice by intraperitoneal injection with a single dose of 220 mg/Kg body weight of alloxan. Animal’s treatment with 100 and 200 mg/Kg of body weight of the aqueous extract was started 34 days after induction of diabetes. Sperm density, morphology and motility were assessed by standard methods. Serum levels of testosterone, FSH, and LH were measured. In addition, the testes were removed for histological study. Proportions of total polyphenols, flavonoids and tannins in the aqueous extract of Cnestis ferruginea were 5260.32 +/- 26 mg EAG /100g, 1384.43 +/- 4 mg EQ /100g and 7380.95 +/- 121 mg EC /100g respectively. 100 and 200 mg/Kg of body weight doses induced highly significant (P ˂ .001) reductions of 68% and 71% in blood glucose levels respectively, significant (P ˂ .05) increases in testes weight of 9% and 10% respectively, highly significant (P ˂ .001) increases in testosterone, pituitary gonadotropins and sperm parameter levels. In addition, regeneration of seminiferous tubules and interstitial cells was observed. Cnestis ferruginea leaves are rich in phenolic compounds. These compounds have anti-diabetic and fertilizing activities on the male reproductive system of diabetic mice.

scholarly journals Antioxidant Evaluation of the Aqueous Extract of Hulls of Campsiandra laurifolia in Colitis Induced by Acetic Acid in Wistar Rats

2021 ◽  
Vol 41 (02) ◽  
pp. 138-144
Author(s):  
Lucas Petitemberte de Souza ◽  
Henrique Sarubbi Fillmann ◽  
Sandielly Rebeca Benitez da Fonseca ◽  
Marilda da Silva Brasil ◽  
Norma Anair Possa Marroni ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Antioxidant Activities ◽  
Experimental Colitis ◽  
Anal Pressure ◽  
Antioxidant Power ◽  
Significance Level ◽  
Sphincter Anal

AbstractDue to the ethnopharmacological use of Campsiandra laurifolia (Fabaceae), popularly known as Acapurana, to treat wounds and ulcers, associated with the lack of alternative treatments for intestinal inflammations such as ulcerative colitis (UC), the present work sought to characterize its phytochemical and antioxidant activities, and to evaluate remedial action in experimental colitis with acetic acid. Phytochemical analyzes were performed through qualitative and quantitative colorimetric tests of the main secondary metabolites. In the colitis model, 24 male Wistar rats aged ± 60 days old were used, divided into 4 groups: Control (CO) control + aqueous extract of C. laurifolia 50 mg/kg (CO + A50); Colitis (CL); and Colitis + aqueous extract of C. laurifolia 50 mg/kg (CL + A50). Measurement of sphincter anal pressure and histological tests of the large intestine, lipoperoxidation (LPO), enzyme activity of superoxide dismutase (SOD), and levels of glutathione (GSH) were performed. For statistical analysis, the oxidative stress (OS) results were expressed as means ± standard error, adopting a significance level of p < 0.05. The screening indicated the presence of flavonoids, saponins and tannins in the extract, with high levels of phenolic compounds and tannins, and was related to high antioxidant capacity. In the histological analysis, the CL group presented loss of the crypts, edema and inflammatory infiltrate. The use of C. laurifolia extract restructured the crypts, decreased edema and increased sphincter anal pressure, with a decrease in LPO, SOD, and an increase in GSH. It is suggested that the use of C. laurifolia extract reduces OS due to its antioxidant power conferred by the phenolic compounds present in the extract.

scholarly journals Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3303
Author(s):  
Amina M. G. Zedan ◽  
Mohamed I. Sakran ◽  
Omar Bahattab ◽  
Yousef M. Hawsawi ◽  
Osama Al-Amer ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Individual Treatment ◽  
Alcoholic Extract ◽  
Anticancer Effect ◽  
Mcf7 Cells ◽  
Vespa Orientalis ◽  
Anti Inflammatory

The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2; (2) suppression of intracellular reactive oxygen species; (3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1; (4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1; and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.

scholarly journals Compared Phenolic Compound Contents of 22 Commercial Fruit and Vegetable Juices: Relationship to Ex-Vivo Vascular Reactivity and Potential In Vivo Projection

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 92 ◽  
Author(s):  
Alexis Matute ◽  
Jessica Tabart ◽  
Jean-Paul Cheramy-Bien ◽  
Bernard Pirotte ◽  
Claire Kevers ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Vascular Reactivity ◽  
Ex Vivo ◽  
Clinical Investigation ◽  
Epigallocatechin Gallate ◽  
Fruit Juices ◽  
Organ Bath ◽  
Total Polyphenol ◽  
Total Anthocyanin

The real impact of polyphenol-rich vegetable and fruit juice intake on cardiovascular health remains a matter of controversy. In the present study, rat aorta segments immersed in an organ bath (OB) were used to explore whether the total polyphenol content and/or individual phenolic compound contents of 22 commercial vegetable (n = 3) and fruit juices [(citrus (n = 5), berries (n = 10), apple (n = 2), pineapple (n = 2)] might be associated with vascular tone. Red juices (particularly blackcurrant) and lemon juice caused the most marked vasorelaxation, its amplitude being endothelium dependent or not according to the volume ratio of juice to initial OB solution Vjuice/VOBS). At volume ratios 5% and 10%, both the juice and OB total polyphenol for all juices and total anthocyanin contents for berry juices significantly correlated with aorta vasorelaxation intensity. This was not the case for total or individual flavonols (except kaempferol) or for total or individual flavanols (except epigallocatechin gallate). If one relates our measured concentrations of individual phenolic compounds in OB to what is known about their physiological concentrations, and given our evidenced correlations between compound concentrations and vasorelaxation intensity, kaempferol, epigallocatechin gallate and peonidin-3-O-glucoside seem to emerge as the interesting phenolic compounds likely to be responsible for the potent vasorelaxation observed with fruit juices, and more particularly blackcurrant ones. Clinical investigation is required, however, to confirm our observations.

scholarly journals Persicarin isolated from Oenanthe javanica protects against diabetes-induced oxidative stress and inflammation in the liver of streptozotocin-induced type 1 diabetic mice

2017 ◽  
Vol 13 (4) ◽  
pp. 1194-1202 ◽  
Author(s):  
Joo Young Lee ◽  
Min Yeong Kim ◽  
Sung Ho Shin ◽  
Mi-Rae Shin ◽  
O Jun Kwon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Mice ◽  
Oenanthe Javanica

scholarly journals A Phenolic Fraction from Catharanthus roseus L. Stems Decreases Glycemia and Stimulates Insulin Secretion

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Janet Alejandra Espejel-Nava ◽  
Elisa Vega-Avila ◽  
Francisco Alarcon-Aguilar ◽  
Alejandra Contreras-Ramos ◽  
Guadalupe Díaz-Rosas ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Catharanthus Roseus ◽  
Chromatographic Analysis ◽  
Hypoglycemic Effect ◽  
Diabetic Mice ◽  
Rinm5f Cells ◽  
Phenolic Fraction

Catharanthus roseus (L.) G. (C. roseus) is a medicinal plant used traditionally for diabetes mellitus control. Several compounds of an alkaloidal nature have been proposed as hypoglycemic principles. However, little attention has been paid to other compounds in this plant that could also participate in this hypoglycemic activity. This study aimed to analyze the hypoglycemic effect of a polyphenolic fraction from C. roseus, as well as its action on insulin secretion and expression in RINm5F cells. Methods. An alkaloid-free aqueous extract was obtained from C. roseus stems. The hypoglycemic effect of different doses of this extract was evaluated in normal and streptozotocin-induced diabetic mice. This extract was fractionated by bipartition, and the resultant fractions were assessed by their hypoglycemic effects. Subsequently, the fraction with the greater hypoglycemic activity was added to the RINm5F cells, and the expression and secretion of insulin were analyzed. The antioxidant activity was determined by the DPPH method and through chromatographic analysis of the most active fraction by HPLC, using an Econosphere C18 column. Results. The aqueous alkaloid-free extract of C. roseus stems significantly reduced blood glucose in normal and diabetic mice. The fractionation of this extract provided three fractions, one of which (a precipitate) showed significant reductions in glycemia at 6 h (48.1 and 64.5% in normal and diabetic mice, respectively). This precipitate contained phenolic compounds and saponins. Its chromatographic analysis showed that it is formed by several phenolic compounds; gallic acid (0.053%) and chlorogenic acid (0.216%) were identified and quantified. Conclusion. The phenolic fraction of C. roseus containing gallic acid and chlorogenic acid had a hypoglycemic effect that may be explained by an increase in insulin secretion.

scholarly journals Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Anita Agnieszka Śliwińska ◽  
Katarzyna Sykłowska-Baranek ◽  
Anita Kośmider ◽  
Sebastian Granica ◽  
Karolina Miszczak ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Cytotoxic Activity ◽  
Phenolic Compound ◽  
Plant Extracts ◽  
Detrimental Effect ◽  
A549 Cells ◽  
Ferulic Acids

<p>In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of <em>Polyscias filicifolia</em> was developed. The phenolic compound content in <em>P. filicifolia</em> has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of <em>P. filicifolia</em> extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.</p><p>The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.</p>

scholarly journals Phenolic compound, nutritional and antioxidant profile of pollen collected by the genus melipona in North Eastern Brazil

2019 ◽  
Vol 22 ◽  
Author(s):  
Rosane Gomes de Oliveira ◽  
Sona Jain ◽  
Lisiane dos Santos Freitas ◽  
Edilson Divino de Araújo
Keyword(s):  
Amino Acids ◽  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Cinnamic Acid ◽  
Antioxidant Property ◽  
Food Supplement ◽  
Nutritional Composition ◽  
Northeastern Brazil ◽  
Total Phenolic ◽  
Eastern Brazil

Abstract The pollen collected by eusocial bees is often reported as being healthy food due to its important nutritional and therapeutic properties. However, studies reporting such properties are rare, especially for pollen collected by the genus Melipona in northeastern Brazil, which is the focus of this research. Pollen from seven species of stingless bees was analysed for its nutritional composition (sugar, lipid, protein and amino acids). The phenolic compound profile was described based on fourteen phenolic compounds (apigenin, kaempferol, luteolin, naringin, rutin, gallic acid, ferulic acid, caffeic acid, p-coumaric acid, chlorogenic acid, abscisic acid, protocatechuic acid, vanillic acid and trans-cinnamic acid). The antioxidant property was analysed by quantifying of total phenolic compounds, total flavonoids and DPPH. Chromatographic methods were used to identify and quantify the phenolic compounds and amino acids. The pollen samples from the bees under study showed good concentrations of proteins and amino acids and good antioxidant potential. The phenolic compounds luteolin, trans-cinnamic acid and apigenin were identified and described in pollen for the first time. Of the amino acids analysed, asparigine, glutamic acid, leucine and proline showed the highest concentrations. The research related to the theme showed that this is one of the first studies to identify and quantify the phenolic compounds and amino acids in stingless bee pollen, reflecting its importance in therapeutic use and as a food supplement.

scholarly journals The effect of a fungal preparation on the tissue cultures of Holarrhena anfidysenterica (Roxb.) Wall.

2014 ◽  
Vol 62 (1-2) ◽  
pp. 43-45 ◽  
Author(s):  
Barbara Dohnal ◽  
Wanda Kisiel
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Culture Medium ◽  
Tissue Cultures ◽  
Unknown Compound ◽  
Holarrhena Antidysenterica ◽  
Phenolic Constituent ◽  
Methyl Ferulate

The addition of an aqueous extract from fruitbodies of <i>Tylopilus felleus</i> to tissue cultures of <i>Holarrhena antidysenterica</i> (<i>Apocynaceae</i>) caused the accumulation of an unknown compound in the culture medium. The compound was isolated and identified as 5-hydroxymethyl-2-furancarboxaldehyde (1). Moreover, biosynthesis of phenolic compounds was stimulated in response to the stress agents of the fungal preparation. Methyl ferulate (2) was the major phenolic constituent.

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