scholarly journals Development of an in-line magnetometer for flow chemistry and its demonstration for magnetic nanoparticle synthesis

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Maximilian O. Besenhard ◽  
Dai Jiang ◽  
Quentin A. Pankhurst ◽  
Paul Southern ◽  
Spyridon Damilos ◽  
...  
Keyword(s):  
Quality Control ◽  
Magnetic Nanoparticles ◽  
High Throughput ◽  
High Throughput Screening ◽  
Magnetic Nanoparticle ◽  
Nanoparticle Synthesis ◽  
Flow Chemistry ◽  
Continuous Quality ◽  
Highly Sensitive

A highly sensitive magnetometer for flow chemistry to characterise magnetic nanoparticles in solution, in situ and in real-time is presented. This facilitates continuous quality control and high-throughput screening of magnetic nanoparticle syntheses.

scholarly journals Integration of an In Situ MALDI-Based High-Throughput Screening Process: A Case Study with Receptor Tyrosine Kinase c-MET

2017 ◽  
Vol 22 (10) ◽  
pp. 1203-1210 ◽  
Author(s):  
Katrin Beeman ◽  
Jens Baumgärtner ◽  
Manuel Laubenheimer ◽  
Karlheinz Hergesell ◽  
Martin Hoffmann ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Maldi Ms ◽  
Kinase Assay ◽  
Label Free ◽  
Screening Process ◽  
Label Free Detection ◽  
Ic50 Values

Mass spectrometry (MS) is known for its label-free detection of substrates and products from a variety of enzyme reactions. Recent hardware improvements have increased interest in the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for high-throughput drug discovery. Despite interest in this technology, several challenges remain and must be overcome before MALDI-MS can be integrated as an automated “in-line reader” for high-throughput drug discovery. Two such hurdles include in situ sample processing and deposition, as well as integration of MALDI-MS for enzymatic screening assays that usually contain high levels of MS-incompatible components. Here we adapt our c-MET kinase assay to optimize for MALDI-MS compatibility and test its feasibility for compound screening. The pros and cons of the Echo (Labcyte) as a transfer system for in situ MALDI-MS sample preparation are discussed. We demonstrate that this method generates robust data in a 1536-grid format. We use the MALDI-MS to directly measure the ratio of c-MET substrate and phosphorylated product to acquire IC50 curves and demonstrate that the pharmacology is unaffected. The resulting IC50 values correlate well between the common label-based capillary electrophoresis and the label-free MALDI-MS detection method. We predict that label-free MALDI-MS-based high-throughput screening will become increasingly important and more widely used for drug discovery.

scholarly journals Novel Benchtop Magnetic Particle Spectrometer for Process Monitoring of Magnetic Nanoparticle Synthesis

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2277
Author(s):  
Norbert Löwa ◽  
Dirk Gutkelch ◽  
Ernst-Albrecht Welge ◽  
Roland Welz ◽  
Florian Meier ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Magnetic Nanoparticles ◽  
Process Monitoring ◽  
Magnetic Nanoparticle ◽  
Magnetic Particle ◽  
Nanoparticle Synthesis ◽  
Separation Processes ◽  
Magnetic Detector ◽  
Application Fields

Magnetic nanoparticles combine unique magnetic properties that can be used in a variety of biomedical applications for therapy and diagnostics. These applications place high demands on the magnetic properties of nanoparticles. Thus, research, development, and quality assurance of magnetic nanoparticles requires powerful analytical methods that are capable of detecting relevant structural and, above all, magnetic parameters. By directly coupling nanoparticle synthesis with magnetic detectors, relevant nanoparticle properties can be obtained and evaluated, and adjustments can be made to the manufacturing process in real time. This work presents a sensitive and fast magnetic detector for online characterization of magnetic nanoparticles during their continuous micromixer synthesis. The detector is based on the measurement of the nonlinear dynamic magnetic response of magnetic nanoparticles exposed to an oscillating excitation at a frequency of 25 kHz, a technique also known as magnetic particle spectroscopy. Our results underline the excellent suitability of the developed magnetic online detection for coupling with magnetic nanoparticle synthesis based on the micromixer approach. The proven practicability and reliability of the detector for process monitoring forms the basis for further application fields, e.g., as a monitoring tool for chromatographic separation processes.

Purifying the Masses:  Integrating Prepurification Quality Control, High-Throughput LC/MS Purification, and Compound Plating To Feed High-Throughput Screening

2005 ◽  
Vol 7 (2) ◽  
pp. 210-217 ◽  
Author(s):  
John J. Isbell ◽  
Yingyao Zhou ◽  
Christina Guintu ◽  
Matthew Rynd ◽  
Shumei Jiang ◽  
...  
Keyword(s):  
Quality Control ◽  
High Throughput ◽  
High Throughput Screening ◽  
The Masses

scholarly journals A simple setup miniaturisation with multiple benefits for Green Chemistry in nanoparticle synthesis

2021 ◽  
Author(s):  
Jette Mathiesen ◽  
Susan Cooper ◽  
Andy Anker ◽  
Tiffany Kinnibrugh ◽  
Kirsten Jensen ◽  
...  
Keyword(s):  
Green Chemistry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Nanoparticle Synthesis ◽  
Nmr Analysis ◽  
Alternative Approach ◽  
Glass Tubes ◽  
Wet Chemical

The development of nanomaterials often relies on wet-chemical syntheses performed in reflux setups using round-bottom-flasks. An alternative approach to synthesise nanomaterials is presented that uses glass tubes designed for NMR analysis as reactors. It uses less solvent, generates less waste, provides safer conditions, is less prone to contamination and is compatible with high throughput screening.

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