Atmospheric pressure plasma liquid assisted deposition of polydopamine/acrylate copolymer on zirconia (Y-TZP) ceramics: a biocompatible and adherent nanofilm

The first two authors contributed equally to this study. Bioactivity and cell adhesion properties are major factors for fabricating medical devices such as coronary stents. The aim of this study was to evaluate the advantages of atmospheric-pressure plasma jet in enhancing the biocompatibility and endothelial cell-favorites. The experimental objects were divided into before and after atmospheric-pressure plasma jet treatment with the ratio of nitrogen:argon = 3:1, which is similar to air. The treated surfaces were basically characterized by means of a contact angle analyzer for the activation property on their surfaces. The effect of atmospheric-pressure plasma jet on cellular response was examined by endothelial cell adhesion and XTT analysis. It was difficult to detect any changeable morphology after atmospheric-pressure plasma jet treatment on the surface. The roughness was increased after atmospheric-pressure plasma jet treatment compared to nonatmospheric-pressure plasma jet treatment (86.781 and 7.964 nm, respectively). The X-ray photoelectron spectroscopy results showed that the surface concentration of the C–O groups increased slightly from 6% to 8% after plasma activation. The contact angle dramatically decreased in the atmospheric-pressure plasma jet treated group (22.6 ± 15.26°) compared to the nonatmospheric-pressure plasma jet treated group (72.4 ± 15.26°) ( n = 10, p < 0.05). The effect of the increment in hydrophilicity due to the atmospheric-pressure plasma jet on endothelial cell migration and proliferation was 85.2% ± 12.01% and 34.2% ± 2.68%, respectively, at 7 days, compared to the nonatmospheric-pressure plasma jet treated group (58.2% ± 11.44% in migration, n = 10, p < 0.05). Taken together, the stent surface could easily obtain a hydrophilic property by the atmospheric-pressure plasma jet method. Moreover, the atmospheric-pressure plasma jet might affect re-endothelialization after stenting.

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An atmospheric-pressure plasma-treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal-derived osteoblastic cells

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.33114 ◽

2014 ◽

Vol 102 (6) ◽

pp. 1289-1296 ◽

Cited By ~ 11

Author(s):

Tomoyuki Kawase ◽

Takaaki Tanaka ◽

Hiromi Minbu ◽

Mana Kamiya ◽

Masafumi Oda ◽

...

Keyword(s):

Cell Adhesion ◽

Atmospheric Pressure ◽

Titanium Surface ◽

Atmospheric Pressure Plasma ◽

Osteoblastic Cells ◽

Initial Cell ◽

Pressure Plasma ◽

Initial Cell Adhesion

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Atmospheric pressure plasma induced grafting of poly(ethylene glycol) onto silicone elastomers for controlling biological response

Journal of Colloid and Interface Science ◽

10.1016/j.jcis.2012.02.052 ◽

2012 ◽

Vol 375 (1) ◽

pp. 193-202 ◽

Cited By ~ 18

Author(s):

Raechelle A. D’Sa ◽

Jog Raj ◽

M. Ann S. McMahon ◽

David A. McDowell ◽

George A. Burke ◽

...

Keyword(s):

Ethylene Glycol ◽

Atmospheric Pressure ◽

Biological Response ◽

Atmospheric Pressure Plasma ◽

Poly Ethylene Glycol ◽

Silicone Elastomers ◽

Pressure Plasma ◽

Poly Ethylene

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Acquisition of cell-adhesion capability of the surface of crosslinked albumin films irradiated with atmospheric-pressure plasma jets

Japanese Journal of Applied Physics ◽

10.7567/jjap.55.07lg03 ◽

2016 ◽

Vol 55 (7S2) ◽

pp. 07LG03 ◽

Cited By ~ 3

Author(s):

Tatsuru Shirafuji ◽

Mami Iwamura ◽

Ryosuke Taga ◽

Yukiyasu Kashiwagi ◽

Kota Nakajima ◽

...

Keyword(s):

Cell Adhesion ◽

Atmospheric Pressure ◽

Atmospheric Pressure Plasma ◽

Plasma Jets ◽

Pressure Plasma

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Effect of Plasma Treatment of Titanium Surface on Biocompatibility

Applied Sciences ◽

10.3390/app9112257 ◽

2019 ◽

Vol 9 (11) ◽

pp. 2257 ◽

Cited By ~ 6

Author(s):

Daiga Ujino ◽

Hiroshi Nishizaki ◽

Shizuo Higuchi ◽

Satoshi Komasa ◽

Joji Okazaki

Keyword(s):

Bone Marrow ◽

Cell Adhesion ◽

Atmospheric Pressure ◽

Titanium Surface ◽

Pure Titanium ◽

Atmospheric Pressure Plasma ◽

Bone Differentiation ◽

Pressure Plasma ◽

Initial Adhesion ◽

Rat Bone

It was recently reported that implant osseointegration is affected by surface wettability. The relationship between hydrophilicity and cell adhesion was corroborated by numerous in vivo studies. Concentrated alkali improves the biocompatibility of pure titanium. Research was conducted on the mechanism by which this treatment increases hydrophilicity. In the present study, we used atmospheric pressure plasma processing to enhance the hydrophilicity of the material surface. The aim was to assess its influences on the initial adhesion of the material to rat bone marrow and subsequent differentiation into hard tissue. Superhydrophilicity was induced on a pure titanium surface with a piezobrush, a simple, compact alternative to the conventional atmospheric pressure plasma device. No structural change was confirmed by Scanning electron microscope (SEM) or scanning probe microscopy (SPM) observation. X-ray photoelectron spectroscopy (XPS) analysis presented with hydroxide formation and a reduction in the C peak. A decrease in contact angle was also observed. The treated samples had higher values for in vitro bovine serum albumin (BSA) adsorption, rat bone marrow (RBM) cell initial adhesion, alkaline phosphatase activity (ALP) activity tests, and factors related to bone differentiation than the untreated control. The present study indicated that the induction of superhydrophilicity in titanium via atmospheric pressure plasma treatment with a piezobrush affects RBM cell adhesion and bone differentiation without altering surface properties.

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Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo

Applied Sciences ◽

10.3390/app11156836 ◽

2021 ◽

Vol 11 (15) ◽

pp. 6836

Author(s):

Yeon-Jee Yoo ◽

Min-Ji Kang ◽

Hiran Perinpanayagam ◽

Joo-Cheol Park ◽

Seung-Ho Baek ◽

...

Keyword(s):

Cell Adhesion ◽

Cell Viability ◽

Real Time ◽

Atmospheric Pressure ◽

Dental Pulp ◽

Atmospheric Pressure Plasma ◽

Pressure Plasma ◽

Group I ◽

Human Dental Pulp ◽

Root Dentin

This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (n = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (p < 0.05), without interactions with EDTA (p > 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.

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