scholarly journals Use of CRISPR/Cas9 technology efficiently targetted goat myostatin through zygotes microinjection resulting in double-muscled phenotype in goats

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Zhengyi He ◽  
Ting Zhang ◽  
Lei Jiang ◽  
Minya Zhou ◽  
Daijin Wu ◽  
...  
Keyword(s):  
Protein Metabolism ◽  
Muscle Hypertrophy ◽  
Adipocyte Differentiation ◽  
Body Weight Gain ◽  
Muscle Growth ◽  
Pcr Analysis ◽  
Wild Type ◽  
Myostatin Gene ◽  
Average Body ◽  
Average Body Weight

Myostatin gene (MSTN) can inhibit the proliferation of myoblast, which in turn promotes muscle growth and inhibits adipocyte differentiation in livestock. MSTN mutation may lead to muscle hypertrophy or double-muscled (DM) phenotype. MSTN mutation animal, such as sheep, dog, and rabbit have been generated through CRISPR/Cas9 technology. However, goats with promising MSTN mutation have not been generated. We designed two sgRNAs loci targetting exon3 of MSTN gene to destroy the MSTN cysteines knots. We got seven goats from seven recipients, in which six were MSTN knocked-out (KO) goats, with a mutation rate of 85.7%. Destroyed cysteine knots caused MSTN structure inactivation. The average body weight gain (BWG) per day of MSTN KO goats was significantly higher than that of wild-type (WT) goats. MSTN KO goats showed abnormal sugar, fat, and protein metabolism compared with wild-type controls (MSTN+/+). Inheritance of mutations was observed in offspring of MSTN KO goats by PCR analysis.

scholarly journals Myostatin Gene Expression and Its Application on Goat Breeding Programme

2018 ◽  
Vol 27 (2) ◽  
pp. 89 ◽  
Author(s):  
Aron Batubara
Keyword(s):  
Muscle Hypertrophy ◽  
Body Weight Gain ◽  
Muscle Growth ◽  
Single Strand Conformation Polymorphism ◽  
Molecular Techniques ◽  
Chain Reaction ◽  
Myostatin Gene ◽  
Production Parameters ◽  
Polymerase Chain ◽  
Conformation Polymorphism

Characteristics of double muscled growth in animals are influenced by myostatin gene (MSTN). Myostatin gene is known as a member of the growth gene's superfamily (TGF-β) which works to suppress the muscle growth. However, the presence of six mutations on MSTN cause the gene inactive, and trigger the occurrence of muscle hypertrophy. Identification of myostatin gene was conducted by molecular techniques, and the most common method is polymerase chain reaction followed by single-strand conformation polymorphism (PCR-SSCP). Research on sheep and goat in several countries showed that there had been several variations occurred in myostatin gene but further studies are required to correlate these variations to body weight gain and other important production parameters. For goat production in Indonesia, myostatin mutations cause double muscling that can be utilised for genetic improvement in goat breeding plan to produce a new breed with high quality meat.

THE EFFECT OF (Morinda citrifolia L.) MEAL IN DIET ON PERFORMANCE OF BROILER

2012 ◽  
Vol 2 (1) ◽  
pp. 21-27
Author(s):  
Yosi Fenita
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Morinda Citrifolia ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Randomized Design ◽  
Different Levels ◽  
Effect Of Feeding ◽  
Average Body ◽  
Average Body Weight

The objective of the research was to evaluate to effect of feeding mengkudu on performances of broilers. The research design used was completely randomized design. One hundred broilers were distributed into five treatments. The treatments were different levels of mengkudu meal (0, 0.75%, 1.5%, 2.25 % and 3%). The observed measured were feed consumption, average body weight (gain) and feed conversion. Results showed that feeding mengkudu (Morinda Citrifolia L.)  no effect significant (P>0.05) on feed consumption, average body weight and feed conversion.  In conclusion, feeding mengkudu meal up to 3% (in diet) does not negatively affect feed consumption, average body weight, and feed conversion.

scholarly journals The Effects of Feed Color on Broiler Performance between Day 1 and 21

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1511
Author(s):  
Joseph P. Gulizia ◽  
Kevin M. Downs
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Treatment Effects ◽  
Body Weight Gain ◽  
Experimental Period ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Broiler Performance ◽  
Average Body ◽  
Average Body Weight

Two trials were conducted to determine feed color effects on broiler performance. A completely randomized design was used. Trial 1 included four treatments: control (complete broiler starter diet), red, green, and blue; and Trial 2 included four treatments: control, orange, yellow, and purple. Each trial had 4 treatments with 4 replicates (60 birds/treatment) fed to 240 male Cobb 500 broilers during a 21 d grow out. Data were analyzed using the GLM procedure. In Trial 1, there were no treatment effects on average body weight, body weight gain, and feed consumption (p > 0.05). Adjusted feed conversion for control (1.23) was less than red (1.27; p = 0.001) and green (1.26; p = 0.009), with blue (1.25; p = 0.056) tending to be different during the experimental period. In Trial 2, there were no treatment effects on average body weight, feed consumption, and adjusted feed conversion during this study (p > 0.05). Body weight gain between d 1 to 14 for purple (490.78 g/bird) was more than orange (467 g/bird; p = 0.013) and yellow (461 g/bird; p= 0.004), with control (474 g/bird; p = 0.052) tending to be different. Results indicate that these feed colors had some, albeit limited, influence on broiler performance parameters.

scholarly journals AMPKγ3 is dispensable for skeletal muscle hypertrophy induced by functional overload

2016 ◽  
Vol 310 (6) ◽  
pp. E461-E472 ◽  
Author(s):  
Isabelle Riedl ◽  
Megan E. Osler ◽  
Marie Björnholm ◽  
Brendan Egan ◽  
Gustavo A. Nader ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Signaling Pathways ◽  
Muscle Hypertrophy ◽  
Muscle Growth ◽  
Mammalian Target Of Rapamycin ◽  
Growth Control ◽  
Mass Gain ◽  
Mtor Signaling ◽  
Wild Type ◽  
Skeletal Muscle Hypertrophy

Mechanisms regulating skeletal muscle growth involve a balance between the activity of serine/threonine protein kinases, including the mammalian target of rapamycin (mTOR) and 5′-AMP-activated protein kinase (AMPK). The contribution of different AMPK subunits to the regulation of cell growth size remains inadequately characterized. Using AMPKγ3 mutant-overexpressing transgenic Tg-Prkag3 225Q and AMPKγ3-knockout ( Prkag3−/−) mice, we investigated the requirement for the AMPKγ3 isoform in functional overload-induced muscle hypertrophy. Although the genetic disruption of the γ3 isoform did not impair muscle growth, control sham-operated AMPKγ3-transgenic mice displayed heavier plantaris muscles in response to overload hypertrophy and underwent smaller mass gain and lower Igf1 expression compared with wild-type littermates. The mTOR signaling pathway was upregulated with functional overload but unchanged between genetically modified animals and wild-type littermates. Differences in AMPK-related signaling pathways between transgenic, knockout, and wild-type mice did not impact muscle hypertrophy. Glycogen content was increased following overload in wild-type mice. In conclusion, our functional, transcriptional, and signaling data provide evidence against the involvement of the AMPKγ3 isoform in the regulation of skeletal muscle hypertrophy. Thus, the AMPKγ3 isoform is dispensable for functional overload-induced muscle growth. Mechanical loading can override signaling pathways that act as negative effectors of mTOR signaling and consequently promote skeletal muscle hypertrophy.

scholarly journals Effect of calf starter on the growth performance of dairy calves

2020 ◽  
Vol 5 (2) ◽  
pp. 60-64
Author(s):  
Rezaul Mahmud Nahid ◽  
Md Anwar Hossain ◽  
Mst Latifa Yesmin Camy ◽  
Md Rakibul Hasan ◽  
Shuvendu Sarkar ◽  
...  
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Welfare States ◽  
Dairy Calves ◽  
Mustard Oil ◽  
Common Salt ◽  
Holstein Friesian ◽  
Height Gain ◽  
Average Body ◽  
Average Body Weight

Dairy calves must be fed appropriately to meet their nutritional needs, supporting optimal growth and development to achieve the recommended target age at first calving (AFC) of 24 months. Traditional restricted milk feeding practices suppress growth, contribute to negative welfare states and may result in malnutrition and immunosuppression. In this study a total of eight high yielding calves (four Holstein Friesian and four Sahiwal breed) whose average body weight was 44.8 kg and 58 kg respectively. The supplied calf starter was composed of maize crushed, rice polish, wheat bran, gram broken, mustard oil cake, vitamin mineral premix, molasses and common salt. Calf starter was supplied initially 0.25 kg/day/calf in equal halves to the experimental calves twice a day i.e. at 7.00 AM and 12.30 PM and gradually increase upto 1 kg/d/calf. Average body weight gain, wither height gain, body length gain, barrel height gain, and hip height gain in Sahiwal and Holstein Friesian calves were 65.97±1.04 Kg, 31.04±1.13 inch, 32.34±1.46 inch, 12.80±0.71 inch, 35.05±1.27 inch, and 53.61±2.41 Kg, 29.78±1.17 inch, 30.93±1.66 inch 11.78±0.66 inch, 33.44±1.41 inch respectively. Our study concluded to establish a consensus on calf feeding standards which support physiological function, facilitate weaning, support growth targets and ensure calf health and welfare is protected. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (2), 60-64

scholarly journals Performance of EPMp broiler ducks feed with various levels of dietary lysine up to 10 weeks of age

2018 ◽  
Vol 22 (1) ◽  
pp. 1 ◽  
Author(s):  
Maijon Purba ◽  
Arnold P. Sinurat
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Feed Consumption ◽  
Optimum Level ◽  
Feed Conversion ◽  
Poor Growth ◽  
Randomized Design ◽  
Average Body ◽  
Average Body Weight

Determining the optimum level of lysine in the ration will be a significant effort to avoid poor growth in duck husbandry. The purpose of this study was to determine the optimum dietary lysine requirement for EPMp broiler ducks, raised up to 10 weeks of age. The study was designed in a completely randomized design (CRD) with 4 dietary treatments, and 4 replicates. Each replicate consisted of 10 ducks. The 4 treatments were diets, containing lysine: 0.60% (T1); 0.70% (T2); 0.80% (T3) and 0.90% (T4). Variables measured included: feed consumption, body weight gain, feed conversion ratio (FCR), carcass and carcass cuts percentages. The results showed that the performance of 10 weeks of age EPMp broiler duck was significantly affected by the level of dietary lysine (P<0.05). The average body weight gain and the FCR of the duck were significantly affected (P<0.05) by dietary lysine level, but not for feed consumption and percentage of carcass (P>0.05). The average body weight gain of EPMp duck with T4 treatment (0.90% lysine) was significantly higher than that T1 (0.60% lysine) but did not significantly difference (P>0.05) with T2 and T3. The average FCR of EPMp duck under T3 and T4 were significantly (P<0.05) higher than that of EPMp duck under T1. It was concluded that the optimum dietary lysine to produce maximum body weight gain of EPMp duck raised up to 10 weeks of age was 0.70%, while for minimum FCR were at the level of 0.80% and 0.90%.

scholarly journals Experiments confirm a dispersive phenotype associated with a natural gene drive system

2021 ◽  
Vol 8 (5) ◽  
Author(s):  
Jan-Niklas Runge ◽  
Anna K. Lindholm
Keyword(s):  
Sperm Competition ◽  
Local Density ◽  
House Mice ◽  
Gene Drive ◽  
Wild Type ◽  
Fitness Costs ◽  
Oestrus Cycle ◽  
Drive Systems ◽  
Average Body ◽  
Average Body Weight

Meiotic drivers are genetic entities that increase their own probability of being transmitted to offspring, usually to the detriment of the rest of the organism, thus ‘selfishly’ increasing their fitness. In many meiotic drive systems, driver-carrying males are less successful in sperm competition, which occurs when females mate with multiple males in one oestrus cycle (polyandry). How do drivers respond to this selection? An observational study found that house mice carrying the t haplotype, a meiotic driver, are more likely to disperse from dense populations. This could help the t avoid detrimental sperm competition, because density is associated with the frequency of polyandry. However, no controlled experiments have been conducted to test these findings. Here, we confirm that carriers of the t haplotype are more dispersive, but we do not find this to depend on the local density. t -carriers with above-average body weight were particularly more likely to disperse than wild-type mice. t -carrying mice were also more explorative but not more active than wild-type mice. These results add experimental support to the previous observational finding that the t haplotype affects the dispersal phenotype in house mice, which supports the hypothesis that dispersal reduces the fitness costs of the t .

Muscle growth after postdevelopmental myostatin gene knockout

2007 ◽  
Vol 292 (4) ◽  
pp. E985-E991 ◽  
Author(s):  
Stephen Welle ◽  
Kirti Bhatt ◽  
Carl A. Pinkert ◽  
Rabi Tawil ◽  
Charles A. Thornton
Keyword(s):  
Muscle Mass ◽  
Gene Knockout ◽  
Muscle Growth ◽  
Myosin Heavy Chain Isoforms ◽  
Tamoxifen Treatment ◽  
Wild Type ◽  
Pretreatment Level ◽  
Myostatin Gene ◽  
Expression Of Genes ◽  
Genes Encoding

Constitutive myostatin gene knockout in mice causes excessive muscle growth during development. To examine the effect of knocking out the myostatin gene after muscle has matured, we generated mice in which myostatin exon 3 was flanked by loxP sequences (Mstn[f/f]) and crossed them with mice bearing a tamoxifen-inducible, ubiquitously expressed Cre recombinase transgene. At 4 mo of age, Mstn[f/f]/Cre+ mice that had not received tamoxifen had a 50–90% reduction in myostatin expression due to basal Cre activity but were not hypermuscular relative to Mstn[w/w]/Cre+ mice (homozygous for wild-type myostatin gene). Three months after tamoxifen treatment (initiated at 4 mo of age), muscle mass had not changed from the pretreatment level in Mstn[w/w]/Cre+ control mice. Tamoxifen administration to 4-mo-old Mstn[f/f]/Cre+ mice reduced myostatin mRNA expression to less than 1% of normal, which increased muscle mass ∼25% over the following 3 mo in both male and female mice ( P < 0.005 vs. control). Fiber hypertrophy appeared to be sufficient to explain the increase in muscle mass. The pattern of expression of genes encoding the various myosin heavy-chain isoforms was unaffected by postdevelopmental myostatin knockout. We conclude that, even after developmental muscle growth has ceased, knockout of the myostatin gene induces a significant increase in muscle mass.

scholarly journals Effects of varying dietary protein levels on the performance of laying Japanese quail(Coturnix coturnix japonica) in a semi-arid environment

2021 ◽  
Vol 33 (1) ◽  
pp. 45-52
Author(s):  
S. Babangida ◽  
C. O. Ubosi
Keyword(s):  
Body Weight ◽  
Japanese Quail ◽  
Dietary Protein ◽  
Egg Production ◽  
Body Weight Gain ◽  
Arid Environment ◽  
Protein Levels ◽  
Average Body ◽  
Semi Arid ◽  
Average Body Weight

 Four dietary treatments differing in protein levels were compared to ascertain the protein requirement of laying Japanese quail in a semi-arid environment. One hundred and twenty quails were randomly assigned to four treatments (TI — 22%CR T2 — 20%CP T3 — 18%CP and T4 - I6%CP) in a completely randomized block design Each treatment was replicated thrice with ten quails each. Feed intake, body weight gain, hen day egg production, feed conversion ratio FCR), egg quality and hematological constituents were the response criteria. The study lasted seven weeks. Feed and water were provided ad libitum throughout the experimental period No significant (P> 0.05) effect of dietary protein was observed for feed intake, average body weight gain, hen-day egg production, F CR, dressing percentage and hematological parameters. Average body weight was significantly (P<0,05) enhanced by high dietary protein. Egg weight and Haugh unit were significantly (P<0. 05) better for the groups fed the higher protein levels (20 and 22% CP). Hen-day egg production, albumen index, specific gravity, shell weight and percent shell were not affected (P>0.05) by dietary protein levels. The costs of producing l kg egg in the treatment groups were ₦25926, ₦21007, ₦252.44 and ₦1263./10 for T1, T2, T3 and T4, respectively. It was concluded that about 20%CP is required in the diets of laying Japanese quail under a semi-arid environment of Nigeria.

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