Direct Measurement of the Hepatointestinal Extraction of Zinc in Cirrhosis and Hepatitis

1981 ◽  
Vol 61 (4) ◽  
pp. 441-444 ◽  
Author(s):  
P. W. N. Keeling ◽  
W. Ruse ◽  
J. Bull ◽  
B. Hannigan ◽  
R. P. H. Thompson
Keyword(s):  
Chronic Hepatitis ◽  
Statistical Significance ◽  
Venous Blood ◽  
Zinc Content ◽  
Atomic Absorption Spectrophotometry ◽  
Zinc Extraction ◽  
Blood Samples ◽  
Peripheral Venous Blood ◽  
Absorption Spectrophotometry ◽  
Biopsy Specimens

1. 65Zn was injected intravenously during transjugular liver biopsy and, from simultaneous hepatic and peripheral venous blood samples, hepatointestinal 65Zn extraction was calculated. Hepatic zinc content was measured in biopsy specimens. 2. On the same occasion samples of liver tissue were taken and their zinc content was measured by atomic absorption spectrophotometry. 3. Seven patients with cirrhosis had significantly lower hepatic zinc content and hepatointestinal zinc extraction than six control patients with mild liver disease. Six patients with chronic hepatitis had a mean hepatointestinal zinc extraction higher than control patients, whereas their mean hepatic zinc content was lower, although the former difference did not achieve statistical significance. 4. These results demonstrate that hepatointestinal extraction of zinc is impaired in cirrhosis, but not in chronic hepatitis.

Plasma Cadmium and Zinc in Human Hypertension

1976 ◽  
Vol 51 (5) ◽  
pp. 483-486 ◽  
Author(s):  
G. S. Thind ◽  
Grace M. Fischer
Keyword(s):  
Significant Positive Correlation ◽  
Venous Blood ◽  
Normal Subjects ◽  
Atomic Absorption Spectrophotometry ◽  
Hypertensive Patients ◽  
Peripheral Venous Blood ◽  
Absorption Spectrophotometry ◽  
Arterial Blood ◽  
The Mean ◽  
Cadmium Zinc

1. Plasma cadmium and zinc were determined by atomic absorption spectrophotometry in inferior venal caval or peripheral venous blood in thirty hypertensive patients and fifteen normal subjects. 2. The mean plasma cadmium in hypertensive patients was significantly higher than in normal control subjects. 3. The plasma cadmium/zinc ratio was significantly greater in hypertensive patients. 4. There was a significant positive correlation between the plasma cadmium/zinc ratio and the mean arterial blood pressure.

Interlaboratory Study of Blood Selenium Determinations

1987 ◽  
Vol 70 (4) ◽  
pp. 664-667
Author(s):  
Tee-Siaw Koh
Keyword(s):  
Hydride Generation ◽  
Atomic Absorption Spectrophotometry ◽  
Bovine Blood ◽  
Blood Samples ◽  
X Ray ◽  
Absorption Spectrophotometry ◽  
Coefficients Of Variation ◽  
The Mean ◽  
Se Deficiency

Abstract Fifty-one laboratories from 14 countries participated in a survey on the determination of selenium (Se) in 8 bovine blood samples with Se concentrations ranging from 0.2 μmol/L (0.016 μg/mL) to 14 μmol/L (1.1 μg/mL). The methods used (and the percentage of participants using each method) were fiuorometry (61), hydride-generation atomic absorption spectrophotometry (AAS) (23), graphitefurnace AAS (6), gas chromatography (4), neutron activation analysis (4), and X-ray fiuorometry (2). There was little difference in the mean Se results obtained by fiuorometry or hydride-generation AAS (P > 0.05). Mean intralaboratory coefficients of variation (CVs) from known replicates ranged from 4 to 14% for all samples. Interlaboratory CVs were related to blood Se concentration and increased to 55% at Se levels below 0.4 μmol/L (0.032 μg/mL). Laboratories that used quality control (QC) schemes had lower interlaboratory CVs than those that did not, but the advantage began to diminish at blood Se concentration below 0.4 μmol/L (0.032 μg/mL). The high interlaboratory CVs, coupled with the false assurance from the low intralaboratory CVs and the ineffectiveness of the QC schemes at blood Se concentrations below 0.4 μmol/L (0.032 μg/mL), are of concern in diagnosis of marginal Se deficiency in livestock where the concentrations of interest are in the range 0.15-0.5 μmol/L (0.012-0.039 μg/mL).

Measurement of 5-Androsten-3β, 17β-diol in Spermatic and Peripheral Venous Blood Samples from the Same Human Subjects by a Radioimmunoassay Method

1978 ◽  
Vol 9 (4) ◽  
pp. 194-200 ◽  
Author(s):  
G. Forti ◽  
E. Calabresi ◽  
P. Giannotti ◽  
D. Borrelli ◽  
P. Gonnelli ◽  
...  
Keyword(s):  
Human Subjects ◽  
Venous Blood ◽  
Blood Samples ◽  
Peripheral Venous Blood ◽  
Radioimmunoassay Method

scholarly journals Diversity of Iron and Zinc Content in Bananas from East and Central Africa

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 320-324
Author(s):  
Michael Pillay ◽  
Robert Fungo
Keyword(s):  
Central Africa ◽  
Germplasm Collection ◽  
Zinc Content ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Fiber Protein ◽  
Fe Content ◽  
Zn Content ◽  
Wide Variability ◽  
Iron And Zinc

Bananas and plantains (Musa sp.) are major staple foods in many developing countries of the world. Although bananas are rich in carbohydrate, fiber, protein, fat, and vitamins A, C, and B6 they are largely deficient of iron (Fe), iodine, and zinc (Zn). A small increase in the micronutrient content of bananas could play a major role in combating disorders that are due to deficiency of mineral micronutrients such as Fe and Zn. The objective of this study was to determine the Fe and Zn content of 47 banana genotypes from a germplasm collection in Uganda using atomic absorption spectrophotometry. The Fe and Zn content showed wide variability and highly significant differences (P < 0.001) within and among the different banana categories selected for this study. The highest average Fe content (1.42 mg/100 g) was found in ‘Saba’ (ABB) while the least Fe content (0.06 mg/100 g) was found in ‘Kikundi’ (AAA). The highest average Zn content (1.21 mg/100 g) among the analyzed accessions was found in ‘Kivuvu’ (ABB) while Zn was not detectable in both ‘Kabucuragye’ (AAA) and ‘Grand Naine’ (AAA). Considering these figures, there is a greater than 20-fold variation in the Fe and Zn levels of the banana genotypes used this study suggesting that genetic improvement of genotypes for enhanced micronutrient levels may be achieved by breeding.

ZINC AND MANGANESE CONTENTS OF MICRO-DISSECTED PANCREATIC ISLETS OF SOME RODENTS

1977 ◽  
Vol 86 (3) ◽  
pp. 570-577 ◽  
Author(s):  
Niilo Havu ◽  
Gillis Lundgren ◽  
Sture Falkmer
Keyword(s):  
Heavy Metals ◽  
Guinea Pig ◽  
Pancreatic Islets ◽  
Guinea Pigs ◽  
Graphite Furnace ◽  
Chinese Hamster ◽  
Zinc Content ◽  
Wistar Rat ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry

ABSTRACT By a flameless atomic absorption spectrophotometry procedure, using a graphite furnace, it was possible to assay the contents of zinc and manganese in micro-dissected pancreatic islets of several rodents. Interest was focused upon the islets of guinea-pigs, due to the fact that guinea-pig insulin lacks a histidine residue in the B10 position of the molecule which normally binds zinc (or other heavy metals) in the hexamer formation, probably involved in the storage of insulin. Both the zinc and manganese contents were too low in the guinea-pig islet parenchyma to be reasonably involved in the storage of insulin in the β-granules. Instead, it was suggested that guinea-pig insulin, like hagfish insulin, might crystallize without access to zinc or other heavy metals. Low zinc and manganese contents were also observed in newborn and diabetic guinea-pigs. The islet zinc content was high in the Wistar rat, the Chinese hamster, and the spiny mouse. No significant amounts of manganese were found in any of these kinds of islet parenchyma.

scholarly journals Dihydro-orotase from Clostridium oroticum. Purification and reversible removal of essential zinc

1985 ◽  
Vol 230 (1) ◽  
pp. 101-108 ◽  
Author(s):  
D W Pettigrew ◽  
R R Bidigare ◽  
B J Mehta ◽  
M I Williams ◽  
E G Sander
Keyword(s):  
Enzyme Activity ◽  
Amino Acid ◽  
Phosphate Buffer ◽  
Specific Activity ◽  
Zinc Content ◽  
Atomic Absorption Spectrophotometry ◽  
Kinetic Properties ◽  
Purification Procedure ◽  
Normal Complement ◽  
Absorption Spectrophotometry

A new purification procedure involving five column-chromatography steps is described for dihydro-orotase (L-5,6-dihydro-orotate amidohydrolase, EC 3.5.2.3) from Clostridium oroticum (A.T.C.C. 25750). The native purified enzyme is a dimer of Mr 102 000 and contains 4.0 +/- 0.3 g-atoms of zinc/mol of dimer. These observations agree with those reported previously [Taylor, Taylor, Balch & Gilchrist (1976) J. Bacteriol. 127, 863-873]. It is conclusively demonstrated that dihydro-orotase is a zinc metalloenzyme. Zinc is reversibly removed by treatment with chelators in phosphate buffer at pH 6.5, as demonstrated by atomic absorption spectrophotometry and decrease of enzyme activity. The specific activity is linearly dependent on zinc content. Addition of ZnSO4 to the chelator-treated enzyme results in regain of the normal complement of zinc and enzyme activity. Kinetic properties of the reconstituted enzyme are indistinguishable from those of the native enzyme. The amino acid composition of the homogeneous enzyme suggests that the zinc atoms occupy different environments.

scholarly journals THE EFFECT OF WET CUPPING THERAPY (AL-HIJAMAH) ON SOME BLOOD COMPONENTS: A COMPARATIVE STUDY

2021 ◽  
Vol 2 (3) ◽  
pp. 124-130
Author(s):  
Adel A. M. Saeed ◽  
Wafa F. S. Badulla ◽  
Galal AK. A. Sheikh
Keyword(s):  
Uric Acid ◽  
Metabolic Diseases ◽  
Statistical Significance ◽  
Venous Blood ◽  
Cupping Therapy ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Healthy Male ◽  
Blood Samples ◽  
Ancient Times

Cupping therapy (CT), also known as (Al-Hijamah) is one of the therapeutic techniques that were practiced in many countries of the world in ancient times and it is still used now. The current study was aimed to evaluate some components of the venous blood and comparing the results with that in the blood withdrawn during cupping therapy (CT). The method was carried out by taking blood samples from the scarified area that were made during CT on the skin and venous blood samples. Eighteen mostly healthy male participants were selected randomly between ages 25-61 years. About 5 ml of blood samples were collected from the vein and cupping site for each participant and biochemical parameters that are blood glucose, uric acid, and cholesterol were analyzed. All studied parameters were high in the cupping blood in comparison with the venous blood where it was noted that cholesterol, triglycerides (TG), as well as low-density lipoproteins (LDL), were statistically significant at (p<0.001) while high-density lipoproteins (HDL), blood sugar and uric acid were statistically significant at (p=0.01), and urea was statistically significant at (p=0.05), while creatinine did not give statistical significance at (p>0.05). The current study is consistent with other studies. CT helps in reducing some biological parameters that may be related to some metabolic diseases, so it could maintaining human health.

Levels of hippocampal calcium and zinc following kindling-induced epilepsy

1985 ◽  
Vol 63 (2) ◽  
pp. 159-161 ◽  
Author(s):  
I. Mody ◽  
J. J. Miller
Keyword(s):  
Dentate Gyrus ◽  
Atomic Absorption ◽  
Granule Cells ◽  
Zinc Content ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry

Hippocampal calcium and zinc content was determined using atomic absorption spectrophotometry in control and commissural-kindled rats. In animals exhibiting 5–10 consecutive motor seizures hippocampal calcium was slightly elevated (356.7 parts per million (ppm), dry weight) but not significantly different from controls (329.8 ppm), whereas the amount of zinc was significantly higher (101.6 ppm) than in nonstimulated animals (88.3 ppm). These results are indicative of certain pathophysiological changes in kindled hippocampi, most likely localized to the granule cells of the dentate gyrus where the bulk of hippocampal zinc is confined.

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