scholarly journals Prospects of fluorescence methods application for monitoring of cyanobacterial cultures in biotechnology

2020 ◽  
Vol 23 ◽  
pp. 02007
Author(s):  
Natalia Yu. Grigoryeva ◽  
Anna A. Liss

Cyanobacteria are photosynthetic microorganisms that possess a high potential for innovative applications in agriculture, food production, cosmeticals, wastewater remediation, biofuels, antioxidative enzymes production, etc. During the industrial cultivation of cyanobacteria several parameters as growth rate, physiological state and algological purity of the culture should be controled permanently. One of the methods that can provide on-line monitoring of cyanobacterial cultures and further process automatization is a fluorescence spectroscopy. In this work several fluorescence techniques are analysed and their possible adaptation for biotechnological applications is suggested.

Author(s):  
N. Yu. Grigoryeva ◽  
A. A. Liss

In the last few decades cyanbacteria became a valuable object of biotechnology. During their industrial cultivation growth rate, physiological state and algological purity of the culture should be controled permanently. One of the methods that can provide on-line monitoring of cyanobacterial cultures is a fluorescence spectroscopy.


2011 ◽  
Vol 26 (S1) ◽  
pp. s96-s97
Author(s):  
T.W. Graham

Liberia's 14 year civil war destroyed domestic agricultural production, veterinary and agricultural education, extension services and domestic food security. These losses severely limited domestic food production, and basic hygiene and sanitation: potable water, abattoirs, cold chain and food storage were greatly diminished. The average Liberian life expectancy fell from 45.8 in 1990 to 41.8 years presently. The population birth and death rate are two of the highest globally with a resulting population growth rate, of 2.7% per annum; this growth rate requires an immediate and concerted focus on domestic food production to alleviate nutritional inadequacy and hunger, trade imbalances and loss of foreign exchange credits. Food supply nationally is presumed adequate because of importation, though domestic production is inadequate. Unequal distribution precludes food security for all Liberians. Value chain augmentation, enhancing food availability across all sectors of Liberian society and ensuring distribution of a safe food supply needs critical development. Infant mortality remains one of the highest in the world (approximately 160/1000 births), much of which is attributed to food insecurity, food contamination and lack of uniformly available potable water. Recreation of Liberia's public health and food security requires redevelopment of disease monitoring and laboratory diagnostic capability to re-establish safe food production and handling practices across all sectors. This will allow determination of endemic disease burden for the principal livestock species: poultry, sheep, goats, cattle and swine. Creation of a national disease surveillance/monitoring system allows for targeted disease intervention, ensuring vaccination for correct serotypes and most critically prevalent diseases. Creation of community level training and support will target intervention of local diseases, but also allow for national prioritization of diseases. Targeting which are most prevalent or most likely to cause production limiting effects will require periodic surveillance, targeted vaccination, and chemotherapeutic intervention and evaluation of therapeutic success.


Author(s):  
J. M. Scott

The physiological rates of a normally omnivorous marine rotifer, Encentrum linnhei, were measured under the steady-state chemostat conditions in which the physiological state of the food-algae was kept constant whilst the rotifer growth rate was changed to preset levels. The specific clearance rate ranged between 50 and 100 μl/μg rotifer C/day (1.5–3.0 μ/rot/day) and varied hyperbolically with growth rate, a similar curve was obtained with the specific ingestion rate which varied between 1–2 μg C/μg rot C/day. A mean respiration rate of 0.45 μg C/μg rot C/day was obtained from oxygen consumption measurements. About 60‰ of ingested energy was found to be egested as paniculate matter and 9–4 °0 dissipated as heat, the latter comparing with a theoretical figure of 4–5‰.From rates, transfer efficiencies were obtained giving a mean net growth efficiency (K2) of 38‰ and a mean overall growth efficiency (K1 of 15‰. A curvilinear increase of Kl with growth rate contrasts with linear and hyperbolic responses found with brachionid rotifers.


Author(s):  
G. A. Nozdrin ◽  
N. A. Gotovchikov ◽  
M. S. Yakovleva ◽  
N. S. Yakovleva ◽  
M. B. Lazareva

The combined effect of homobiotics, probiotics based on recombinant strains of g. Bacillus, & enrofloxacin on the dynamics of body weight gain in geese was studied. According to the principle of steam-analogs, one control and five experimental groups of 10 goslings in each group were formed. Gosyatam experimental groups used homobiotics vetom 15.1; vetom 13.1 incombination with enrofloxacin followed by replacement with a probiotic preparation Vetom 1.2. The absolute body weight and average daily gain in geese under the influence of the studied drugs increases. The severity of the effect depended on the pharmacological composition of the drugs used. The maximum absolute weight gain was observed when using the drugs according to the scheme: vetom 13.1 at doses of 25–50 mg/kg for 16 days, with further use of a 10% enrofloxacin solution at a dose of 0.5 ml/kg for 5 days and in The experiment period was 1.2 at a dose of 50 mg/kg for 16 days. After the cessation of the use of drugs, the growth rate increased for 30 days when prescribing the drugs according to the scheme: veto 13.1 at a dose of 50 mg/kg for 16 days and with further introduction of homobiotic veto 1.2 into the bird in a dose of 50 mg/kg for 16 days. The maximum average daily gain in live weight of geese was observed in the period from the 16th to the 32nd day with the administration scheme: homobiotic vetom 13.1 at a dose of 25 mg/kg of body weight 1 time per day for 5 consecutive days, then every other day for 16 days, then daily 10 % solution of enrofloxacin at a dose of 0.5 ml/kg of body weight for 5 days, then vetom 1.2 at a dose of 25 mg/kg once a day for 16 days in a row. And the maximum average daily gain in the post using period of the drug was observed on the 32nd day of the study according to the scheme: homobiotic veto 15.1 at a dose of 50 mg/kg of body weight 1 time per day for 16 days, then daily with vetom 1.2 at the same dose 1 once a day for 16 days. The studied drugs in the applied doses did not have a toxic effect on the physiological state of geese.


GeoTextos ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Juliana Luiza Barbosa Dias ◽  
Juscelino Eudâmidas Bezerra

<p>O setor agrícola foi severamente afetado pela pandemia de Covid-19, gerando incertezas sobre a produção e a distribuição de alimentos, o que afetou diretamente o trabalho de milhões de agricultores. Este artigo visa a analisar o impacto da pandemia na produção de alimentos em Brasília-DF, especialmente entre os agricultores assentados/acampados da reforma agrária. Para a análise, foram utilizados dados primários obtidos através de um questionário on-line destinado ao público da reforma agrária em áreas rurais das regiões administrativas da capital federal, além de dados secundários, disponibilizados por instituições públicas. Os resultados mostraram que o impacto da pandemia foi intenso entre os agricultores, deixando-os extremamente vulneráveis, principalmente nos meses iniciais da pandemia. Como forma de enfretamento dos efeitos da pandemia, os trabalhadores adotaram quatro medidas específicas: a utilização do serviço de entrega delivery; a venda de cestas agroecológicas; a participação em redes solidárias e a venda direta em feira de reforma agrária. Essas novas dinâmicas de comercialização têm transformado as formas de interação campo-cidade ao demandar estratégias diferenciadas para alcançar os consumidores finais, contribuindo para amenizar os efeitos disruptivos da pandemia nos sistemas alimentares locais.</p><p><span>Abstract</span></p><p>IMPACTS OF COVID-19 ON FOOD PRODUCTION AND COMMERCIALIZATION IN BRASILIA-DF: CHALLENGES FOR AGRARIAN REFORM SETTLERS/CAMPED</p><p>The agricultural sector was severely affected by the pandemic of Covid-19, genera- ting uncertainties about food production and distribution, which directly affected the work of millions of farmers. This article aims to analyze the impact of the pandemic on food production in Brasília-DF, especially among farmers settled in agrarian reform settlements. The analysis used primary data obtained through an on-line questionnaire addressed to the public of agrarian reform in rural areas of the administrative regions of the federal capital, as well as secondary data made available by public institutions. The results showed that the impact of the pandemic was intense among farmers, leaving them extremely vulnerable, especially in the initial months of the pandemic. As a way of countering the effects of the pandemic, workers have adopted four specific measures: the use of delivery services; the salof agro-ecological baskets; participation in solidarity networks; and direct sales at agrarian reform fairs. These new commercialization dynamics have transformed the forms of countryside-city interaction by demanding different strategies to reach final consumers, contributing to mitigate the pandemic’s disruptive effects on local food systems.</p>


Author(s):  
Filali R ◽  
◽  
Tian H ◽  
Micheils E ◽  
Taidi B ◽  
...  

Microalgae are photosynthetic microorganisms with many potential applications in the food, cosmetics, pharmaceutical and environmental industries. Currently, commercial microalgae production remains limited. Therefore, improving the growth and the culture density of the microalgae cultivation is one of the key enablers to open the way to mass production and commercialisation of these microorganisms. The effect of culture pH on the photoautotrophic growth of C. vulgaris over a large range of values has been investigated in pH-regulated cultures. For each microalgae culture, the specific growth rate, the cell density, the chlorophyll content, the intracellular carbon content and the nitrogen source consumption were monitored. Optimal growth and carbon incorporation have been observed at pH of 7.0. The fastest growth rate and highest biomass production of C. vulgaris were 0.074 h-1 and 0.896 g/L respectively. Under these conditions, a maximum carbon content of cells was 49 % (w/w).


Fuel ◽  
2015 ◽  
Vol 145 ◽  
pp. 109-115 ◽  
Author(s):  
T. Izida ◽  
L. Bussler ◽  
J.R. Silva ◽  
L.H.C. Andrade ◽  
E. Simionatto ◽  
...  

2020 ◽  
Vol 117 (10) ◽  
pp. 5525-5531 ◽  
Author(s):  
Taiki Miki ◽  
Tsugumichi Shinohara ◽  
Silvia Chafino ◽  
Sumihare Noji ◽  
Kenji Tomioka

Insects living in the temperate zone enter a physiological state of arrested or slowed development to overcome an adverse season, such as winter. Developmental arrest, called diapause, occurs at a species-specific developmental stage, and embryonic and pupal diapauses have been extensively studied in mostly holometabolous insects. Some other insects overwinter in the nymphal stage with slow growth for which the mechanism is poorly understood. Here, we show that this nymphal period of slow growth is regulated by temperature and photoperiod through separate pathways in the cricket Modicogryllus siamensis. The former regulates the growth rate, at least in part, through the insulin / target of rapamycin (TOR) signaling pathway. Lower temperature down-regulates the expression of insulin-like peptide (Ms’Ilp) and Target of rapamycin (Ms’Tor) genes to slow down the growth rate without affecting the number of molts. The latter regulates the number of molts independent of temperature. Short days increase the number of molts through activation of the juvenile hormone (JH) pathway and down-regulation of myoglianin (Ms’myo), a member of the TGFβ family, which induces adult metamorphosis. In contrast, long days regulate Ms’myo expression to increase during the fifth to sixth instar to initiate adult metamorphosis. When Ms’myo expression is suppressed, juvenile hormone O-methyl transferase (Ms’jhamt) was up-regulated and increased molts to prolong the nymphal period even under long-day conditions. The present findings suggested that the photoperiod regulated Ms’myo, and the JH signaling pathway and the temperature-controlled insulin/TOR pathway cooperated to regulate nymphal development for overwintering to achieve seasonal adaptation of the life cycle in M. siamensis.


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