Morphological and anatomical features of narrow-leaved lavender plants with prolonged conservation under in vitro genebank

Optimization of plants long-term conservation under in vitro conditions and identification of their structural and functional features during preservation is the basis for the creation of a genebank of cultured and wild plant species in vitro. The aim of our work was to study morphological and anatomical features of plants in lavender cultivar ‘Sineva’ after two years of in vitro storage at low positive temperatures. The plant material was evaluated after 24 months of conservation. A significant decrease in the linear parameters of leaves in the preserved plants was noted. Leaf shape altered from narrowly linear to oblong. With prolonged storage, the thickness of the leaf blade slightly increased. The integumentary tissues were thinner, compared with the plants cultured under standard in vitro conditions, the cuticle was not clear. The number of stomata decreased. The number of small vascular bundles decreased. The obtained data demonstrate viability of in vitro preserved explants, despite significant differences in structure, preservation of chlorophyll-containing tissues, a decrease in transpiration, and an increase of trichomes number. The stability of the vegetative organs functioning was revealed.

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Performance of Polyester-Based Electrospun Scaffolds under In Vitro Hydrolytic Conditions: From Short-Term to Long-Term Applications

Nanomaterials ◽

10.3390/nano9050786 ◽

2019 ◽

Vol 9 (5) ◽

pp. 786 ◽

Cited By ~ 3

Author(s):

Oscar Gil-Castell ◽

José David Badia ◽

Jordi Bou ◽

Amparo Ribes-Greus

Keyword(s):

Molar Mass ◽

Pure Water ◽

Phosphate Buffer Solution ◽

Short Term ◽

Buffer Solution ◽

Electrospun Scaffolds ◽

The Stability ◽

Short Time

The evaluation of the performance of polyesters under in vitro physiologic conditions is essential to design scaffolds with an adequate lifespan for a given application. In this line, the degradation-durability patterns of poly(lactide-co-glycolide) (PLGA), polydioxanone (PDO), polycaprolactone (PCL) and polyhydroxybutyrate (PHB) scaffolds were monitored and compared giving, as a result, a basis for the specific design of scaffolds from short-term to long-term applications. For this purpose, they were immersed in ultra-pure water and phosphate buffer solution (PBS) at 37 °C. The scaffolds for short-time applications were PLGA and PDO, in which the molar mass diminished down to 20% in a 20–30 days lifespan. While PDO developed crystallinity that prevented the geometry of the fibres, those of PLGA coalesced and collapsed. The scaffolds for long-term applications were PCL and PHB, in which the molar mass followed a progressive decrease, reaching values of 10% for PCL and almost 50% for PHB after 650 days of immersion. This resistant pattern was mainly ascribed to the stability of the crystalline domains of the fibres, in which the diameters remained almost unaffected. From the perspective of an adequate balance between the durability and degradation, this study may serve technologists as a reference point to design polyester-based scaffolds for biomedical applications.

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The Bordetella Bps Polysaccharide Is Critical for Biofilm Development in the Mouse Respiratory Tract

Journal of Bacteriology ◽

10.1128/jb.00785-07 ◽

2007 ◽

Vol 189 (22) ◽

pp. 8270-8276 ◽

Cited By ~ 79

Author(s):

Gina Parise Sloan ◽

Cheraton F. Love ◽

Neelima Sukumar ◽

Meenu Mishra ◽

Rajendar Deora

Keyword(s):

Whooping Cough ◽

Three Dimensional ◽

Respiratory Pathogens ◽

Adolescents And Adults ◽

The Stability ◽

Inert Surfaces ◽

Biofilm Development

ABSTRACT Bordetellae are respiratory pathogens that infect both humans and animals. Bordetella bronchiseptica establishes asymptomatic and long-term to life-long infections of animal nasopharynges. While the human pathogen Bordetella pertussis is the etiological agent of the acute disease whooping cough in infants and young children, it is now being increasingly isolated from the nasopharynges of vaccinated adolescents and adults who sometimes show milder symptoms, such as prolonged cough illness. Although it has been shown that Bordetella can form biofilms in vitro, nothing is known about its biofilm mode of existence in mammalian hosts. Using indirect immunofluorescence and scanning electron microscopy, we examined nasal tissues from mice infected with B. bronchiseptica. Our results demonstrate that a wild-type strain formed robust biofilms that were adherent to the nasal epithelium and displayed architectural attributes characteristic of a number of bacterial biofilms formed on inert surfaces. We have previously shown that the Bordetella Bps polysaccharide encoded by the bpsABCD locus is critical for the stability and maintenance of three-dimensional structures of biofilms. We show here that Bps is essential for the formation of efficient nasal biofilms and is required for the colonization of the nose. Our results document a biofilm lifestyle for Bordetella in mammalian respiratory tracts and highlight the essential role of the Bps polysaccharide in this process and in persistence of the nares.

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Wild Plants for the Phytostabilization of Phosphate Mine Waste in Semi-Arid Environments: A Field Experiment

Minerals ◽

10.3390/min11010042 ◽

2020 ◽

Vol 11 (1) ◽

pp. 42

Author(s):

Hamza Zine ◽

Sara Elgadi ◽

Rachid Hakkou ◽

Eleni G. Papazoglou ◽

Laila Midhat ◽

...

Keyword(s):

Trace Elements ◽

Field Experiment ◽

Plant Species ◽

Mine Waste ◽

Wild Plant ◽

Arid Environments ◽

Wild Plant Species ◽

Phosphate Mine ◽

Semi Arid

The management of mine waste has become an urgent issue, especially in semi-arid environments. In this context, and with an aim to inhibit the oxidation of the sulfide tailings of the abandoned mine of Kettara in Morocco, a store-and-release (SR) cover made of phosphate mine waste (PW) was implemented. In order to guarantee its long-term performance, phytostabilization by local wild plant species is currently the most effective and sustainable solution. This study aimed to assess the growth performance and phytostabilization efficiency of five local wild plant species to grow on the SR cover made of PW. A field experiment was conducted for two growing seasons (2018 and 2019), without amendments and with the minimum of human care. PW and the aboveground and belowground parts of the studied plant species were collected and analyzed for As, Cd, Cu, Ni, and Zn. The bioconcentration factor (BCF) and translocation factor (TF) were also calculated. Despite the hostile conditions of the mining environment, the five plant species showed promising growth performances as follows: Atriplex semibaccata > Vicia sativa > Launaea arborescens > Peganum harmala > Asparagus horridus. The five plants showed high accumulation capacity of the trace elements, with the highest concentrations in belowground tissue. Principal component analysis distinguished A. semibaccata as having a high concentration of Cu and As, while Asparagus horridus had higher concentrations of Cd and Zn. In contrast, P. harmala, V. sativa, and L. arborescens demonstrated affinity regarding Ni. According to the BCF (<1) and TF (<1), these plant species could be used as effective phytostabilizers of the studied trace elements. The present study showed that local wild plant species have a great potential for the phytostabilization of PW, and could ensure the long-term efficiency of SR cover.

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Leaf morpho-anatomical diversity in Echeveria gigantea (Crassulaceae)

Botanical Sciences ◽

10.17129/botsci.2028 ◽

2019 ◽

Vol 97 (2) ◽

pp. 218

Author(s):

Estela Sandoval-Zapotitla ◽

Daniel M. Martínez-Quezada ◽

Jerónimo Reyes-Santiago ◽

María de los Ángeles Islas-Luna ◽

Ulises Rosas

Keyword(s):

Comparison Group ◽

Leaf Shape ◽

Morphological Variability ◽

Taxonomic Group ◽

Vascular Bundles ◽

Anatomical Features ◽

Morphometric Analyses ◽

Specific Hybridization ◽

Definition Of ◽

The Relationship

Background. Distinguishing species and populations in Crassulaceae is challenging because of the morphological variability and inter-specific hybridization. Currently our understanding of the morphological and anatomical features in Echeveria is poor, and therefore it is difficult to delimit species, and morphotypes within the species. Question. Our objective was to describe the foliar anatomy and the shape of accessions in E. gigantea. We used E. gibbiflora, another species in the series as a comparison group. Comparing the two species allowed us to evaluate the relationship between anatomy and morphology in E. gigantea.Methods. We performed a survey of anatomical features in seven accessions of E. gigantea, and one accession of E. gibbiflora. We obtained epidermal prints, leaf sections, performed geometric and morphometric analyses.Results. We found that 65 % of the anatomical traits are heterogeneous among the taxonomic units. Our analysis showed that E. gibbiflora and some E. gigantea accessions share extensive anatomical similarities. There was variation within the E. gigantea, suggesting that at least one of the accessions is an independent taxonomic group. The traits with the largest contribution to variation between the groups were related to the epidermis, the hypodermis, the type of vascular bundles and the collenchyma associated to the vascular bundles. In addition we quantified the variation in leaf shape. Interestingly, we found correlations between the organ shape and anatomical traits.Conclusion. These analyses provide information about traits towards the morpho-anatomical definition of E. gigantea variation and suggest developmental correlation yet to be explained.

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In vitro activation of HIV RNA expression in peripheral blood lymphocytes as a marker to predict the stability of non-progressive status in long-term survivors

AIDS ◽

10.1097/00002030-199601000-00003 ◽

1996 ◽

Vol 10 (1) ◽

pp. 17-22 ◽

Cited By ~ 5

Author(s):

Anna R. Garbuglia ◽

Roberto Salvi ◽

Antonino Di Caro ◽

Giuseppina Cappiello ◽

Francesco Montella ◽

...

Keyword(s):

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Rna Expression ◽

Blood Lymphocytes ◽

Hiv Rna ◽

In Vitro Activation ◽

The Stability ◽

Long Term Survivors

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Experimental study of the liposomal form of fenoterol after improving the method of obtaining it

Pharmacy Formulas ◽

10.17816/phf34272 ◽

2020 ◽

Vol 2 (2) ◽

pp. 30-37

Author(s):

Tatiana M. Ustinova ◽

Nikolai Vengerovich ◽

Mikhail A. Judin

Keyword(s):

Average Diameter ◽

Free Form ◽

Quantitative Composition ◽

Active Principle ◽

Liposomal Form ◽

Duration Of Action ◽

Second Stage ◽

The Stability

The effect of different concentrations of cryoprotector (sucrose) on the efficiency of fenoterol inclusion in the lipid matrix during lyophilization has been studied. It has been shown that the liposomal form with the content of cryoprotector in the internal environment of liposomes 2.5 % and in the external environment equal to 2 % provides long-term preservation of the drug in the liposome cavity. Under these conditions, it is possible to achieve a monodisperse distribution of particles with an average diameter of 4.281.62 m. The assumed quantitative composition of the cryoprotector ensures the manufacturability of the liposome production process, increases the stability of the lyophilizate structure and prevents the particles from sticking together, ensuring their uniformity. The profile of two-stage release of fenoterol from the liposomal form has been shown in vitro. The first stage of rapid release was characterized by a transition to free form within 15 minutes to 42 % of the encapsulated fenoterol. At the second stage, the active principle was released more slowly for 480 minutes. The model of bronchospasm induced by 1% histamine has shown the advantage of the liposomal form of fenoterol in comparison with its free form in the form of an aqueous solution. Intra-tracheal administration of the liposomal form of fenoterol at a dose of 17 ukg/kg provided for 360 minutes the preservation of external respiratory function at the level of initial values, despite histamine inhalation, while the duration of action of fenoterol did not exceed 120 minutes.

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The stability in vitro of bioactive and immunoreactive LH in human blood and plasma

Journal of Endocrinology ◽

10.1677/joe.0.1170139 ◽

1988 ◽

Vol 117 (1) ◽

pp. 139-145 ◽

Cited By ~ 7

Author(s):

A. Tsatsoulis ◽

K. Mavroudis ◽

J. Frost ◽

A. Lambert ◽

S. M. Shalet ◽

...

Keyword(s):

Human Blood ◽

Prolonged Storage ◽

Human Blood Plasma ◽

Immunological Activity ◽

Human Pituitary ◽

Reference Preparation ◽

Degree Of Stability ◽

The Stability ◽

Mouse Leydig Cell

ABSTRACT The degree of stability in vitro of bioactive and immunoreactive LH in human blood, plasma and serum was examined. Bioactivity and immunoreactivity of LH were assayed by the dispersed mouse Leydig cell assay and by standard radioimmunoassay respectively, using the same reference preparation (first international reference preparation for human pituitary LH 68/40 for immunoassay). Bioactive and immunoreactive LH were stable in blood and plasma at 22 °C for up to 4 and 24 h respectively, and in blood at 4 °C for up to 24 h. There was no loss of biological or immunological LH activity in plasma which had been either snap-frozen and stored at −70 °C, allowed to freeze at −20 °C and stored at that temperature or kept at 4 °C for 24 h and then stored at − 70 °C. Likewise, the levels of LH in plasma and serum which had been stored at either − 20 or − 70 °C and then thawed and refrozen up to four times remained unchanged. In addition, the biological and immunological activity of LH was not affected after vortexing samples of plasma or serum for up to 60 s. Bioactive LH was also stable in plasma samples after prolonged storage (up to 9 months) at either −70 or −20 °C. We conclude that LH bioactivity and immunoreactivity are stable in blood and plasma following a variety of treatments commonly experienced during normal handling of a blood sample after venepuncture. J. Endocr. (1988) 117, 139–145

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Surface and Structural Properties of Medical Acrylonitrile Butadiene Styrene Modified with Silver Nanoparticles

Polymers ◽

10.3390/polym12010197 ◽

2020 ◽

Vol 12 (1) ◽

pp. 197 ◽

Cited By ~ 1

Author(s):

Magdalena Ziąbka ◽

Michał Dziadek ◽

Kinga Pielichowska

Keyword(s):

Silver Nanoparticles ◽

Water Solution ◽

Young Modulus ◽

Acrylonitrile Butadiene Styrene ◽

Spectroscopic Studies ◽

Homogeneous Distribution ◽

The Stability ◽

Butadiene Styrene

Acrylonitrile butadiene styrene/silver nanoparticles (ABS/AgNPs) composites were manufactured through the plastic processing method. Three different matrices were used to obtain polymer and composite samples containing 0.5 wt % and 1.0 wt % of silver nanoparticles, respectively. The aim of this study was to examine physicochemical properties and stability of the materials in the in vitro conditions for two years. The results showed that composites made from amorphous matrices had comparable mechanical properties after incorporation of AgNPs. The values of Young modulus and tensile strength increased after the first and second year of investigation. Silver nanoparticles did not alter the surface parameters—e.g., roughness and contact angle also retained stable values after the in vitro incubation in water solution. The scanning electron observation revealed homogeneous distribution of silver modifier in all the matrices. The 24-month incubation of materials proved the stability of the composites microstructure. The DSC analysis revealed that addition of AgNPs may decrease glass transition temperature of the composite materials which was also reduced after 12 and 24 months of incubation. The attenuated total reflectance–Fourier transform infrared (ATR-FTIR) spectroscopic studies did not indicate significant changes in the ABS matrices either upon their modification with AgNPs or after the long-term testing. The conducted studies proved that all the composites are stable and may be used for a long-term working period.

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INVESTIGATION OF THE IN VITRO REGENERATION OF SOME MEDICAL AND AROMATIC WILD PLANT SPECIES

Applied Ecology and Environmental Research ◽

10.15666/aeer/1504_905914 ◽

2017 ◽

Vol 15 (4) ◽

pp. 905-914 ◽

Cited By ~ 2

Author(s):

B. TUNCER

Keyword(s):

Plant Species ◽

In Vitro Regeneration ◽

Wild Plant ◽

Wild Plant Species

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Tracking long-term functional connectivity maps in human stem-cell-derived neuronal networks by holographic-optogenetic stimulation

10.1101/2021.05.11.443589 ◽

2021 ◽

Author(s):

Felix Schmieder ◽

Rouhollah Habibey ◽

Johannes Striebel ◽

Lars Buettner ◽

Juergen Czarske ◽

...

Keyword(s):

Functional Connectivity ◽

Neuronal Networks ◽

Neurological Diseases ◽

Electrode Arrays ◽

Optogenetic Stimulation ◽

Functional Dynamics ◽

Functional Features ◽

Induced Pluripotent

Neuronal networks derived from human induced pluripotent stem cells (hiPSCs) have been exploited widely for modelling neuronal circuits, neurological diseases and drug screening. As these networks require extended culturing periods to functionally mature in vitro, most studies are based on immature networks. To obtain insights on long-term functional features of human networks, we improved a long-term glia-co-culture culturing protocol directly on multi-electrode arrays (MEA), facilitating long-term assessment of electrical features at weekly intervals. We applied optogenetic stimulation to induce neuronal activity, which resulted in accelerated neuronal responses during network development. Using holographic stimulation with single-cell-resolution, propagating evoked activities of 400 individually stimulated neurons per MEA were traceable, and precise network functional connectivity motifs were revealed. Our integrated holographic optogenetic stimulation platform on MEAs facilitates studying long-term functional dynamics of human neuronal networks in vitro. This is an important step towards establishing hiPSC-derived neurons as profound functional testbeds for basic and biomedical research.

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