scholarly journals Correction of cellular immunity with biferon-C in piglets in case of specific prevention of circovirosis and mycoplasmosis

2020 ◽  
Vol 222 ◽  
pp. 02056
Author(s):  
Aleksey Shakhov ◽  
Sergey Shabunin ◽  
Mariya Zheynes
Keyword(s):  
T Cells ◽  
B Lymphocytes ◽  
Adaptive Immunity ◽  
Metabolic Activity ◽  
Cellular Immunity ◽  
Absolute Number ◽  
T Suppressors ◽  
Corrective Effect ◽  
Helper Activity ◽  
T Helpers

The results of studying the effect of biferon-C containing recombinant porcine interferons -alpha and -gamma on cellular nonspecific and adaptive immunity in piglets in case of specific prevention of circovirosis and mycoplasmosis are presented. The corrective effect of the drug on cellular immunity was stated, manifested by an increase in the absorption and functional and metabolic activity of phagocytes, the relative and absolute number of T- and B-lymphocytes, T-cells with helper activity and a lower formation of the level of T-suppressors, an increase in the T-helpers/T-suppressors ratio.

scholarly journals Cellular immunity of rabbits incase of parasite association (Treponema cuniculi and Eimeria sp.)

2019 ◽  
Vol 21 (96) ◽  
pp. 8-13
Author(s):  
Y. V. Duda
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
B Lymphocyte ◽  
Absolute Number ◽  
Blood Parameters ◽  
Control Group ◽  
The Absolute ◽  
T Helpers ◽  
Experimental Group

Despite a huge number of studies, the uniqueness of antiparasitic immunity is so great that there is still insufficient knowledge of the factors contributing to the manifestation of the characteristics of immunity in mixed parasitic diseases of rabbits. Therefore, the question of the influence of the association of pathogens Treponema cuniculi and Eimeria sp. on indicators of cellular immunity of rabbits is relevant. The study was conducted on 59 male rabbits age 3–5 months of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). Intensity of invasion was determined by the method of the Mac-Master. It has been established that the level of damage of rabbits by spirochetosis and eimeriosis was, on average, 1155.17 ± 184.87 and 6668.97 ± 284.16 pathogens in 1 g of feces. The count of T- and B-lymphocytes was determined by the method of spontaneous rosette-formation with sheep erythrocytes. Parasitizing the association of pathogens Treponema cuniculi and Eimeria sp. was revealed a high number of leukocytes (1.22 times, P < 0.001), which increased mainly due to lymphocytes, which were 1.45 times higher (P < 0.001), as well as neutrophilic metamyelocytes – 1.48 times (P < 0.05), eosinophils – 1.68 times (P < 0.001) and basophils – 1.57 times (P < 0.001) compared with similar blood parameters of healthy animals. In the blood of sick rabbits, the absolute number of T-lymphocytes (1.56 times, P < 0.001) and B-lymphocytes (3.02 times, P < 0.001) was significantly higher in comparison with a low number of O-lymphocytes (3.46 times, P < 0.001) compared with the control. This indicates the redistribution of lymphocytes to cells that carry T and B lymphocyte receptors on the plasma membrane. The absolute number of T-lymphocytes became high due to T-helpers, which in these animals were higher both in absolute (1.87 times, P < 0.001) and percentage (by 9.18%, P < 0.001) compared to control. Moreover, the percentage of T-suppressors in the blood of rabbits of the experimental group was significantly lower on 5.46% (P < 0.05) compared with the same blood count of healthy animals. Such a redistribution of the T-cell population in the peripheral blood of this group of rabbits led to an increase in the immunoregulatory index by 1.64 times (P < 0.01) than in healthy ones. High IRI and the number of T-active lymphocytes (by 28.23%, P < 0.05) in the blood of rabbits with parasitism of the association of pathogens Treponema cuniculi and Eimeria sp. indicate increased immune system tension.

scholarly journals The state of cellular immunity in children with recurrent respiratory diseases

2020 ◽  
pp. 64-74
Author(s):  
O Povorova
Keyword(s):  
T Cells ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Cd8 T Cells ◽  
Respiratory Diseases ◽  
B Lymphocyte ◽  
Correlation Coefficients ◽  
Cytotoxic Lymphocytes ◽  
T Helper ◽  
T Helpers

In children with recurrent respiratory diseases, deviations from the norm of various parameters of the immunity system were determined in more than 85% of cases. The most part of children with indicators outside the range of reference values of the subpopulations of lymphocytes is determined at the age of 3-6 years. In children, a decrease in B lymphocytes (CD19+), as well as T-cytotoxic lymphocytes (СD3+СD8+), is accompanied by an increase in T cells (CD3+) and T helper cells (CD3+CD4+). The most frequent variants of combined disorders of the parameters of the cellular link of immunity were determined: a decrease in B-lymphocytes and an increase in T-cells and T-helpers - in 40.2% (206 out of 512 children); a decrease in B-lymphocytes and a decrease in T-cytotoxic ones - in 42% (215 of 512). Similar correlations between cellular immunity indicators in frequently ill children of Mogilev and Minsk regions were determined: positive Spearman correlation coefficients between CD3/CD4 and CD3/CD8 T- cells, negative-between CD4/CD8 T- cells. The proportion of children with indices of T- and B-lymphocyte subpopulations within the normal range is higher in children of the Minsk region compared with children in the Mogilev region for all the studied subpopulations of lymphocytes (P<0.05). There were no differences in the leukocyte-T-lymphocyte index between the two groups of children.

scholarly journals PSVI-22 Immunity indicators of calves obtained from cows with ketosis during the treatment process

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 199-199
Author(s):  
Sergey Kovalev ◽  
Anastasia Nikitina ◽  
Polina Anipchenko ◽  
Anatoly Stekolnikov ◽  
Pavel Kiselenko ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Treatment Process ◽  
Absolute Number ◽  
Relative Content ◽  
Newborn Calves ◽  
Cellular And Humoral Immunity

Abstract The purpose of these studies was to determine the indicators of cellular and humoral immunity of calves. Compared to calves obtained from untreated cows, the number of T-lymphocytes in young calves born from cows treated with Timolin increased the level of T-cells to 32.9%, while using Timosplenin 45%. As a result of the use of immunomodulators in treating cows with ketosis in mothers, the relative content of T-lymphocytes in the blood of calves born from them increased when using the drug Leucogen by 1.7 times, Timosplenin by 2.3 times, Timalin in 2.2 times. The use of Leucogen for cows with ketosis contributed to an increase in the percentage of B cells in the blood of calves born by 23%, Timosplenin 83%, and Timalin 42%. At the same time, the absolute number of B-lymphocytes in the treatment of “Leucogen” increased 1.9 times and reached the level of healthy calves, in the treatment with the preparations “Timosplenin” and “Timalin” - the increase was, respectively, 3.0 and 2.3 times and was higher this indicator in young animals born from healthy calves. Thus, the inclusion of dry immunomodulators in the treatment regimen of patients with ketosis leads not only to normalization of metabolism, but is also accompanied by an increase in the level of cellular immunity factors in calves born from them. Under the influence of “Leucogen,” the percentage of lymphocytes was normalized, whereas with the introduction of “Timalin” and “Timosplenin,” the content of this type of cells in newborn calves even exceeded those in healthy young.

Physiotherapy Complex for the Treatment of Children with Somatoform Dysfunction, Often Suffering from Recurrent Infection

2020 ◽  
Vol 98 (4) ◽  
pp. 140-148
Author(s):  
N.G. Kulikova ◽  
I.V. Volkova ◽  
A.S. Tkachenko
Keyword(s):  
B Lymphocytes ◽  
Blood Cells ◽  
White Blood Cells ◽  
Recurrent Infection ◽  
Recurrent Infections ◽  
T Suppressors ◽  
Children With Adhd ◽  
Therapeutic Measures ◽  
T Helpers ◽  
Head Area

The aim. to optimize the treatment of children with ADHD who often suffer from recurrent infections by justifying the effectiveness of a therapeutic and physiotherapy complex that includes a BOS-bioacoustic effect on the head area and NILI on the projection of the cubital area and thymus. Method of research. In children (140 people) with a verified diagnosis of ADHD, often suffering from recurrent infection, humoral-cellular immunity indicators (immunoglobulins: IgA; IgG; IgM); T-and B-lymphocytes; cytokines) were studied in the dynamics of the use of therapeutic measures. Depending on the applied physiotherapy method of treatment, the children were divided into 3 groups: the 1st group-received NILI on the projection of the cubital area and thymus, the 2nd – received BOS-bioacoustic effect on the head area, the 3rd – developed physiotherapy complex, including BOS-bioacoustic effect on the head area and NILI on the projection of the cubital area and thymus. Conclusion. the developed physiotherapy complex, including BOS-bioacoustic effect on the head area and NILI on the projection of the cubital area and thymus, can be considered as a therapeutic and preventive model of respiratory viral attacks in children with SSDVNS, since it reduces the number of recurrent viral attacks by 3.7 times, correcting the activity of circulating cytokines, T-lymphocytes (CD3+), T-helpers (CD4+) and T-suppressors (CD8+) in the population white blood cells.

scholarly journals CLINICAL EFFECTS OF HUMAN IMMUNOGLOBULIN ADMINISTRATION IN WOMEN WITH RHESUS SENSITIZATION AT THE PRE-GRAVID STAGE

2020 ◽  
pp. 126-132
Author(s):  
S. G. Chernievskaya ◽  
N. M. Rozhkovska ◽  
V. G. Marichereda ◽  
T. O. Yermolenko
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Roc Analysis ◽  
Human Immunoglobulin ◽  
Control Group ◽  
Clinical Group ◽  
Clinical Effects ◽  
T Helper ◽  
T Suppressors

The aim of the study – to evaluate changes in cellular immunity in rhesus-sensitized women in response to IVIG administration and the prognostic effectiveness of a method for the prevention of isoimmunization in the next pregnancy. Materials and Methods. The study was performed on the basis of City Maternity Hospital No. 7" (Odesa) in 2014–2019. 37 rhesus-sensitized women were randomly splited in two clinical groups: main clinical group (n=19) where patients received human immunoglobulin for intravenous administration, and control group (n=18) where patients did not receive IVIG. Results and Discussion. The state of cellular immunity in rhesus-sensitized women is characterized by a moderate decrease in the absolute and relative indices of T-lymphocytes while increasing the number of B-lymphocytes. The NK cell population did not differ from the control group. When analyzing subpopulations of T-lymphocytes, it can be concluded that the number of T-helper cells is increased and the number of T-suppressors is proportionally reduced. These changes explain the increase in the number of B-lymphocytes as a result of increasing antigenic load on cell receptors. In the group of women who received IVIG therapy, the ratio of chances of normalization of cellular immunity was 18.41 (95 % CI 2.62–166.74), T-helper – 14.93 (95 % CI) 2.45–107.8), T-suppressors – 14.57 (95 % CI 2.13 –127.57) and B-lymphocytes – 31.87 (95 % CI 4.1–333.41). According to the ROC analysis, the quality of the statistical model of IVIG application corresponds to AUC = 0.843 (95 % CI 0.689–0.941) According to the ROC analysis, the level of β-lymphocytes in the compared AUC groups = 0.58 (95 % CI 0.405–0.742).

Immunological Status of Children Infected with Helicobacter pylori

2017 ◽  
Vol 33 ◽  
pp. 95-102
Author(s):  
Y.G. Romanenko
Keyword(s):  
T Cells ◽  
Helicobacter Pylori ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Immune Complexes ◽  
Peripheral Blood ◽  
Immunological Status ◽  
Highly Active ◽  
Phagocyte Activity ◽  
Cd4 Lymphocytes

Chronic gastritis and duodenitis at the children, associated with Helicobacter pylori infection, carried out by violations in a cellular link of immunity: lymphocytosis, imbalance of subpopulations T-cells with reduction of absolute CD3+, CD4+ - lymphocytes, increasing of absolute measures CD8+ - and CD16+ - lymphocytes. Increasing content of B - lymphocytes, maintenance of IgG and IgM in the peripheral venues blood demonstrates activation of a humoral link on the background of oppression of T - cellular immunity. In the peripheral blood had been shown increase of phagocyte activity of neutrophils, amount of highly active cells, level of the circulating immune complexes.

scholarly journals Sex differences of subpopulation composition of lymphocytes in the peripheral blood in experimental acute and chronic ulcerative colitis

2020 ◽  
Vol 22 (1) ◽  
pp. 157-164
Author(s):  
O. V. Makarova ◽  
E. A. Postovalova ◽  
Yu. Gao ◽  
M. T. Dobrynina
Keyword(s):  
Sex Differences ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Relative Number ◽  
Absolute Number ◽  
Control Group ◽  
Chronic Colitis ◽  
Acute Colitis ◽  
The Absolute ◽  
T Helpers

We studied sex differences lymphocytes subpopulations of peripheral blood in adult C57Bl/6 mice during acute and chronic colitis, induced with 1% DSS. We measured subpopulations of lymphocytes with flow cytometry. We showed that in the control group the female mice had statistically significantly higher values of the relative number of regulatory and cytotoxic T lymphocytes comparing to the males. During acute colitis the females showed an increase in the relative number of Thelpers and a decrease of cytotoxic Tlymphocytes, which reflects the activation of immune response. The males had a decrease in the absolute number of leukocytes, lymphocytes and cytotoxic and regulatory T lymphocytes, probably because of an increase in migration of these cells to the inflammation locus and local lymph nodes. In chronic colitis the females had a decrease in the absolute number of leukocytes, lymphocytes, T helpers, cytotoxic T lymphocytes and B lymphocytes when comparing with acute colitis. During chronic colitis the males had a decrease in the absolute number of T helpers and B lymphocytes but an increase of regulatory T cells in comparison with the control group; in comparison with acute colitis the males with chronic colitis had higher relative and absolute number of regulatory T cells. The increase of T regulatory lymphocytes is due to an increase in their proliferation rate in the thymus and increase of their migration to the inflammatory locus – the colon. Future clinical studies may be based on these results, which show that the treatment of colitis, especially with immunotropic agents, must take sex differences into account.

scholarly journals Dynamics of Cellular Factors of the Immune System of Arctic Foxes (Vulpes lagopus) on the Background of Mixtinvasion

2019 ◽  
Author(s):  
Yuri Kuznetsov ◽  
Nadezhda Kuznetsova
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Natural Resistance ◽  
The Arctic ◽  
Control Group ◽  
Vulpes Lagopus ◽  
T Suppressors ◽  
Arctic Foxes ◽  
Cellular Factors ◽  
T Helpers

The article presents data on the dynamics of cellular immune system factors of the arctic foxes (Vulpes lagopus) while being affected by mixtinvasion of protozoa (Isospora vulpina) and helminth (Toxascaris leonina). The changes in the blood cell composition, in particular those responsible for the body’s immunity and immune status, reflect the pathological effect of the endoparasites on the animal’s body. The development of the invasive process during mixtinvasions causes and is accompanied by a decrease in the level of nonspecific resistance of the host organism. In addition, a significant process of inhibition of the lysozyme complementary activity in the blood serum and the development of secondary immunodeficiencies is observed. The aim of our work was to study the development of the pathological process in arctic foxes, caused by previously detected mixtinvasion by eimeriidoses and helminthiases, as well as to identify dynamical patterns of the immune system cellular factors. Intravital diagnostics of parasitoses was carried out by means of coproscopic studies with the help of conventional flotation methods. 127 arctic foxes participated in the experiment. Among them individuals with double invasion, an invasion with both protozoa (placeI. vulpina) and helminth (T. leonina), were selected for the experiment. Healthy animals served as the control group. Arctic foxes which took part in the experiment were divided into three groups of 10 animals each. Groups were formed by the method of balanced analogous groups. Evaluation of the T-system of immunity was carried out by the method of spontaneous rosetting according to Jondal (1972). Isolation of lymphocytes by the method of A. Boyum (1968), B-lymphocytes were determined by the method of E. Mendes (1973), theophylline-resistant and theophylline-sensitive T-lymphocytes were determined using the method of S. Limatibul et.al. (1978). The immunoregulation index was calculated by the ratio of T-helpers to T-suppressors. During the experiment, it was found that in infected animals the total number of lymphocytes was significantly 10.2 % higher than in animals in the control group, however, there was a tendency to a sharp decrease in the total number of lymphocytes in patients with I. vulpina + T. leonina. The T-helper dynamics in infected foxes was 18.9 ± 0.9 (P≤0.05), which is 21.9 % less than in the control – 24.2 ± 0.6 (P≤0, 05). The dynamics of T-suppressors turned out to be directly opposite to the dynamics of the T-helpers. The dynamics of B-lymphocytes in the 2nd group turned out to be 1.76 times higher than in animals from the control. In the 3rd group same indicator was comparable with the control values of 2.9 ± 0.3, against 2.5 ± 0.4 in the control group. The study showed that mixtinvasion with placeI. vulpina + T. leonina leads to immunosuppression in the arctic foxes. Interestingly the specific therapy did not straighten the immune state of the animals, but on the contrary it became more pronounced, which suggests that antiparasitic drugs have an immunosuppressive nature on the body of arctic foxes as evidenced by violations of the parameters of the body’s natural resistance, the content of T-E-ROCK lymphocytes.

scholarly journals Продуктивність та неспецифічна резистентність курчат-бройлерів за дії β-каротину

2017 ◽  
Vol 7 (3) ◽  
pp. 90-95 ◽  
Author(s):  
L.V. Shevchenko ◽  
O.S. Yaremchuk ◽  
V.M. Mykhalska
Keyword(s):  
Vitamin A ◽  
B Lymphocytes ◽  
T Helper Cells ◽  
Phagocytic Activity ◽  
Broiler Chickens ◽  
Absolute Number ◽  
T Helper ◽  
T Suppressors ◽  
Helper Cells ◽  
Β Carotene

<p>Use in feeding broiler chickens of Vitaton and Vitadeps with and without butylhydroxytoluene (BHT) improves feed intake and water, and has no effect on body weight. The feeding of broiler chickens with mix fodder of Vitaton and BHT in doses that meet and exceed the physiological need of β-carotene calculated for retinol and Vitaton with no OSH in a normal dose of β-carotene did not significantly alter the absolute number of lymphocytes blood and the ratio of their subpopulation responsible for cellular (T-lymphocytes) and humoral (B-lymphocytes) immunity. Phagocytic activity of neutrophils and phagocytic index were not significantly changed in the peripheral blood of broiler chickens at feeding Vitaton as a source of β-carotene with/without BHT. Use of Vitaton without BHT in feeding of broiler chickens at a dose of 0.7 g/kg caused a lymphocytopenia. In chickens of this group we noted a decrease in the total number of lymphocytes in the blood due to decrease of cellular and humoral immunity, namely all subpopulations of T-lymphocytes, including T-helper cells - by 58.3 %, T-suppressors - by 41.0, T-helper cells active - at 43.5, B-lymphocytes by 60.1 %, and 0-lymphocytes – by 40.0 %. This is consistent with the reduction in titer of natural antibodies in their serum by 35.3 %. This phagocytic activity of neutrophils and index of phagocytes in blood of broiler chickens unchanged compared with the control. Use of Vitadeps in feeding broiler chickens at a dose of 5.6 g/kg in mix fodder also caused lymphocytopenia. This was reflected in the decrease of absolute number of blood 0-lymphocytes by 43.4 %, T-suppressors - by 38.7 % and T-helper cells active - by 39.0 %, but their ratio in the blood was like control level. Thus, usage of Vitaton and Vitadeps with/without butylhydroxytoluene in doses that meet the bird demands of β-carotene calculated to vitamin A, ensure the normal functioning of immune organs. Use of Vitaton with BHT as a source of β-carotene for the feeding of broiler chickens at a dose exceeding the normal content of vitamin A by 7 times, does not cause the violations of immunological homeostasis of the poultry. The Vitaton without butylhydroxytoluene and Vitadeps in doses exceeding the normal content of bird β-carotene calculated to vitamin A by 7 times depressing the immunopoesis in broiler chickens.</p>

scholarly journals Adaptive differentiation of murine lymphocytes. IV. (Responder × Nonresponder) F(1) T cells can be taught to preferentially help nonresponder,rather than responder, B cells

1979 ◽  
Vol 150 (1) ◽  
pp. 20-30 ◽  
Author(s):  
DH Katz ◽  
LR Katz ◽  
CA Bogowitz ◽  
PH Maurer
Keyword(s):  
T Cells ◽  
B Cells ◽  
B Lymphocytes ◽  
Cell Interaction ◽  
Parental Cell ◽  
Helper T Cells ◽  
Parental Type ◽  
Helper Cells ◽  
Native Cell ◽  
Helper Activity

Responses to the synthetic terpolymer L-glutamic acid, L-lysine, L-tyrosine (GLT) in the mouse are controlled by H-2-1inked Ir-GLTgenes. (Responder × nonresponder) F(1) hybrid mice, themselves phenotypic responders, can be primed with GLT to develop specific helper cells capable of interacting with 2,4-dinitrophenyl hapten (DNP)-primed F(1) B cells in response to DNP-GLT. Unlike the indiscriminant ability of F(1) helper T cells for conventional antigens (i.e. not Ir gene-controlled), which can help B cells of either parental type (as well as F(1)) equally well, GLT-primed F(1) T cells can only provide help under normal circumstances for B lymphocytes of responder parent origin; they are unable to communicate effectively with nonresponder parental B cells (1, and the present studies). The present studies reveal, however, that the induction of a parental cell-induced allogeneic effect during priming of F(1) mice to GLT actually dictates the direction of cooperating preference that will be displayed by such F(1) helper cells for B cells of one parental type or the other. Thus, F(1) T cells, primed to GLT under the influence of an allogeneic effect induced by parental BALB/c cells, develop into effective helpers for nonresponder A/J B cells, but fail to develop effective helpers for responder BALB/c B cells, and vice-versa. In contrast, F(1) T cells, primed to GLT under the influence of an allogeneic effect induced by either parental type, display significantly enhanced levels of helper activity for B cells derived from F(1) donors. These results are interpreted to reflect the existence of two interdependent events provoked by the allogeneic effect: one event augments the differentiation of GLT-specific helper T cells belonging to the subset corresponding to the opposite parental type; this would explain the development of increased helper activity provided to partner B cells of opposite parental type (as well as of F(1) origin). The second event, we postulate, involves the production of responses against the receptors which normally self-recognize native cell interaction determinants; this form of anti-idiotype response is restricted against self- recognizing receptors of the same parental type used for induction of the allogeneic effect, hence explaining diminished helper activity of such F(1) cells for partner B lymphocytes of corresponding parental type.

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