Increasing content of lipid in tropical microalgae Chlorella sorokiniana and Closterium sp. with variation of nitrogen content and extraction temperature

This research was aimed to obtain algae biofuels as an alternative energy which comes from tropical microalgae biomass Chlorella sorokiniana and Closterium sp. The cultivation was performed at a controlled room in batch culture photobioreactor, temperature 27°C, pH 6, aeration with air flow rate of 150 mL/sec, and light intensity at 2400 lux. These nutrient sources used artificial PHM with KNO3 variation as a nitrogen source of 0 grams; 0.25 grams; 0.5 grams; 1 gram. The research involved microalgae lipid extraction with Blight & Dyer method and also carried out support of alcohol, chloroform and distilled water (1:1:1) with optimum temperature 30oC. Oil yields that obtained were analyzed with Gas Chromatography Mass Spectrometry (GC-MS) method, dry weight with a gravimetric method and cell density with spectrophotometry. The result showed that the reduction of KNO3 materials (0,25 gr) can produce biomass and highest total lipid content at 0,41 g/L and 20,31 %/(w/w). The results showed that KNO3 decreased the amount of biomass which was not significant but the total lipid content of microalgae was increased. Lipid content and fatty acids extracted from Blight & Dyer showed oil content that could potentially be the raw material of biodiesel.

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Adipophilin and Perilipin 3 Correlate with Total Lipid Content in Human Breast Milk

10.20944/preprints201810.0008.v1 ◽

2018 ◽

Author(s):

Tereza Pavlova ◽

Zdenek Spacil ◽

Veronika Vidova ◽

Filip Zlamal ◽

Eliska Cechova ◽

...

Keyword(s):

Mass Spectrometry ◽

Breast Milk ◽

Total Lipid ◽

Lipid Content ◽

Gravimetric Method ◽

Total Lipid Content ◽

Milk Lipid ◽

Human Breast Milk ◽

Human Breast

Objective: Lipids are secreted into milk as bilayer-coated structures: milk lipid globules (MLGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MLGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The aim of the study was to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations and dynamics during lactation. Research Methods & Procedures: Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1–3, 12–14, 29–30, 88–90 and 178–180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. Results: We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first 6 months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. Moreover, we derived an equation for estimating the mean lipid content of milk based on ADRP concentration in human breast milk. Conclusions: A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.

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Increasing lipid content from biomass of microalgae to produce biofuels with optimization of nitrogen source

MATEC Web of Conferences ◽

10.1051/matecconf/201819713011 ◽

2018 ◽

Vol 197 ◽

pp. 13011

Author(s):

Istikhomah Putri Ayuwaningsih ◽

Melati Ferianita Fachrul ◽

Astri Rinanti

Keyword(s):

Nitrogen Source ◽

Lipid Content ◽

Scenedesmus Obliquus ◽

Gravimetric Method ◽

Total Lipid Content ◽

Dry Weight ◽

Gas Chromatography Mass Spectrometry ◽

Green Microalgae ◽

Urea Fertilizer ◽

Operational Conditions

The aim of this research is to analyze the effect of urea fertilizer as a nitrogen source to lipid productivity of mixed culture of green microalgae consisting of Monoraphidium sp., Chlorella sorokiniana, and Scenedesmus obliquus as lipid sources. In research, cultures were cultivated in a 1.5 L glass photobioreactor with batch culture system. The operational conditions of this research were carried out at pH 6.0, 27 °C, aeration with air flow 150 mL/sec, and 2400 lux with vary amount of urea as a source of nitrogen as much (grams) 0.0; 0.5; 1.0. The Bligh and Dyer extraction is performed to produce biofuels after harvesting process and to analyze lipid content. Analysis of fatty acids using Gas Chromatography Mass Spectrometry (GCMS) Method, analysis of dry weight using Gravimetric Method, and analysis of cell density using Spectrofotometry Method. This research concludes that with 0.5 grams of urea fertilizer can produce dry weight and total lipid content optimally that were 0.26% (w/w) and 36,35% (w/w). This research concludes that increasing amount of nitrogen source could be increasing green microalgae biomass but is not for increased lipid content. The high lipid content can be produced by decreasing 50% nitrogen source.

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Impact of Seasonal Changes on Composition of Total Lipid Content in Muscle, Liver and Testes of Freshwater Male Fish Mystus Cavasius (Ham) From Bhadra Reservoir, Shivamogga, Karnataka, India

International Journal of Scientific Research ◽

10.15373/22778179/feb2014/181 ◽

2012 ◽

Vol 3 (2) ◽

pp. 540-541

Author(s):

Ashashree H M Ashashree H M ◽

◽

B.Venkateshwarlu B B.Venkateshwarlu B ◽

Sayeswara HA Sayeswara HA

Keyword(s):

Total Lipid ◽

Lipid Content ◽

Seasonal Changes ◽

Total Lipid Content ◽

Male Fish

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Aspergillus flavusgrowth and aflatoxin production as influenced by total lipid content during growth and development of cottonseed

Journal of Crop Improvement ◽

10.1080/15427528.2016.1263811 ◽

2017 ◽

Vol 31 (1) ◽

pp. 91-99 ◽

Cited By ~ 2

Author(s):

Kanniah Rajasekaran ◽

Greg Ford ◽

Kandan Sethumadhavan ◽

Carol Carter-Wientjes ◽

John Bland ◽

...

Keyword(s):

Total Lipid ◽

Lipid Content ◽

Growth And Development ◽

Total Lipid Content ◽

Aflatoxin Production

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Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-013-0920-8 ◽

2013 ◽

Vol 37 (2) ◽

pp. 99-106 ◽

Cited By ~ 45

Author(s):

Geun Ho Gim ◽

Jung Kon Kim ◽

Hyeon Seok Kim ◽

Mathur Nadarajan Kathiravan ◽

Hetong Yang ◽

...

Keyword(s):

Biomass Production ◽

Total Lipid ◽

Lipid Content ◽

Total Lipid Content ◽

Culture Conditions ◽

Green Microalgae

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The Use of Urea and Kelp Waste Extract is A Promising Strategy for Maximizing the Biomass Productivity and Lipid Content in Chlorella sorokiniana

Plants ◽

10.3390/plants9040463 ◽

2020 ◽

Vol 9 (4) ◽

pp. 463 ◽

Cited By ~ 1

Author(s):

Ali Nawaz Kumbhar ◽

Meilin He ◽

Abdul Razzaque Rajper ◽

Khalil Ahmed Memon ◽

Muhammad Rizwan ◽

...

Keyword(s):

Lipid Content ◽

Fossil Fuels ◽

Fossil Fuel ◽

Lipid Production ◽

Total Lipid Content ◽

Biomass Productivity ◽

Chlorella Sorokiniana ◽

Biofuel Production ◽

Promising Strategy ◽

Waste Extract

The decline in fossil fuel reserves has forced researchers to seek out alternatives to fossil fuels. Microalgae are considered to be a promising feedstock for sustainable biofuel production. Previous studies have shown that urea is an important nitrogen source for cell growth and the lipid production of microalgae. The present study investigated the effect of different concentrations of urea combined with kelp waste extract on the biomass and lipid content of Chlorella sorokiniana. The results revealed that the highest cell density, 20.36 × 107 cells−1, and maximal dry biomass, 1.70 g/L, were achieved in the presence of 0.5 g/L of urea combined with 8% kelp waste extract. Similarly, the maximum chlorophyll a, b and beta carotenoid were 10.36 mg/L, 7.05, and 3.01 mg/L, respectively. The highest quantity of carbohydrate content, 290.51 µg/mL, was achieved in the presence of 0.2 g/L of urea and 8% kelp waste extract. The highest fluorescence intensity, 40.05 × 107 cells−1, and maximum total lipid content (30%) were achieved in the presence of 0.1 g/L of urea and 8% kelp waste extract. The current study suggests that the combination of urea and kelp waste extract is the best strategy to enhance the biomass and lipid content in Chlorella sorokiniana.

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