scholarly journals The Production of Bioethanol from Rimau Gerga Lebong (Rgl) Orange Waste as an Alternative Energy

2018 ◽  
Vol 248 ◽  
pp. 04004
Author(s):  
Vike Darliyasi ◽  
Kurnia Herlina Dewi ◽  
Budiyanto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alternative Energy ◽  
Block Design ◽  
Trichoderma Viride ◽  
Total Dissolved Solids ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Ethanol Content ◽  
Significant Difference ◽  
Orange Waste

Bioethanol from Rimau Gerga Lebong (RGL) orange waste is one of the solution to overcome fuel oil problem. The aim of this research is to get the type of microorganisms and fermentation time that produce the highest ethanol from RGL orange waste. The research method used was Randomized Block Design (RBD) of two factors, namely type of microorganisms (Trichoderma viride, Saccharomyces cerevisiae, and Trichoderma viride + Saccharomyces cerevisiae) and fermentation time (3 days, 5 days, and 7 days. Within the three type of microorganisms with variations of fermentation time showed that the pH was able to carry out the fermentation process smoothly. The highest total dissolved solids were in the type of Trichoderma viride 3 days and 5 days, and the type of mix of microorganisms on the 3rd day. The highest ethanol content is in the type of Sachharomyces cerevisiae for 7 days. ANOVA result showed that the interaction between two treatments on the total dissolved solids experienced significant differences, so it continue with the DMRT test at a significant level of 0.5%. However, it is different from the results of ANOVA on ethanol content which showed that there were significant differences between ethanol content and types of microorganisms, but there was no significant difference on fermentation time

scholarly journals Effect of Saccharomyces cerevisiae ATCC 9763 concentration and fermentation time on bioethanol content from corn stover crude cellulose substrate

2021 ◽  
Vol 913 (1) ◽  
pp. 012026
Author(s):  
A M M Napitupulu ◽  
L Suhendra ◽  
I B W Gunam
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Corn Stover ◽  
Ph Value ◽  
Block Design ◽  
Sugar Content ◽  
Total Dissolved Solids ◽  
Reducing Sugar ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Ethanol Content

Abstract Corn stover is a waste from the corn plant that dried in the fields after the corn cobs were harvested. From many corn wastes produced, there is very little utilization to corn stover. Delignified corn stover contains 65.46% cellulose, 14.58% hemicellulose, and 8.66% lignin. Lignocellulosic biomass is very difficult to biotransform, therefore it must be delignified to break the bonds between cellulose, hemicellulose, and lignin. Then the cellulose is converted into sugars by saccharification using crude cellulose enzymes so it can be converted into bioethanol through a fermentation process using simultaneous saccharification and fermentation (SSF) method. This study aims to determine the concentration of Saccharomyces cerevisiae ATCC 9763 and the optimum fermentation time in order to obtain high content of bioethanol from corn stover. Bioethanol production at different concentrations of S. cerevisiae and fermentation time uses a factorial randomized block design (RBD) consisting of two factors. The first factor was the concentration of S. cerevisiae which consisted of 3 levels, namely 3%, 5%, and 7% (v/v). The second factor was the fermentation time which consists of 4 levels, namely 24 hours, 48 hours, 72 hours, and 96 hours. Observed variables included pH value, total dissolved solids, reducing sugar content, and ethanol content. The data obtained were analyzed for its diversity using analysis of variants (ANOVA) and continued with the HSD Tukey. The results showed that the concentration of S. cerevisiae 7% (v/v) and a fermentation time of 96 hours was the best treatment to obtain a maximum ethanol content of 7.53 ± 0.330 g/L, with a final pH value of 4.25 ± 0.07, total dissolved solids 2.9 ± 0.14 °Bx and reducing sugar content of 0.334 ± 0.03 g/L. Increasing the concentration of S. cerevisiae and fermentation time can increase the ethanol content.

scholarly journals Pengaruh Penambahan Ragi Tape dan Waktu Fermentasi Hasil Samping Cairan Pulpa terhadap Karakteristik Mutu Cuka Kakao (Theobroma cacao L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 177
Author(s):  
Ida Bagus Gede Awidyanata ◽  
G.P Ganda Putra ◽  
Luh Putu Wrasiati
Keyword(s):  
Acetic Acid ◽  
Block Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Alcohol Content ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Natural Fermentation ◽  
Acid Ph ◽  
Cocoa Bean Fermentation

The aim of this study was to determine the effect of adding ragi tape and the time of natural fermentation of the pulp byproducts of the fermented cocoa beans on the characteristics of the quality of cocoa vinegar and determining the addition of tape yeast and the natural fermentation time of the best pulp fluid for cocoa vinegar production. The experiments in this study used a randomized block design with two factors. The first factor is the addition of yeast tape consisting of 5 levels, namely without the addition of yeast tape (control), the addition of yeast tape 0.05% (w / v), 0.10% (w / v), 0.15% (w / v), 0.20% (w / v), the second factor is the fermentation time consisting of 6 levels, namely 5, 10, 15, 20, 25, 30 days. Treatment The addition of tape yeast has a very significant effect on acetic acid, pH, total dissolved solids, total sugar, has no significant effect on alcohol content in the fermented vinegar from the pulp fluid which is the by-product of cocoa bean fermentation. The length of fermentation treatment has a very significant effect on acetic acid, pH, total dissolved solids, total sugar, alcohol. The interaction between the two treatments had no significant effect on acetic acid, pH, total dissolved solids, alcohol content, and significant effect on total sugar in fermented cocoa vinegar products. The best treatment was obtained from the addition of 0.15% (w / v) yeast tape and 30 days fermentation time which produced cacao vinegar with acetic acid content of 2.40%, pH 3.37, total dissolved solids 4.25 obrix, total sugar 0.03%, and 0.00% alcohol. Keywords  : vinegar fermentasion, ragi tape, time of fermentation

scholarly journals Production of bioethanol from wild cassava crude starch (Manihot glaziovii Muell. Arg) using different microbial types and fermentation times

2021 ◽  
Vol 913 (1) ◽  
pp. 012032
Author(s):  
S V Mellicha ◽  
I B W Gunam ◽  
N S Antara ◽  
I W Arnata
Keyword(s):  
Ph Value ◽  
Block Design ◽  
Sugar Content ◽  
Dry Weight ◽  
Total Dissolved Solids ◽  
Reducing Sugar ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Cassava Plant ◽  
Manihot Glaziovii

Abstract Wild cassava (Manihot glaziovii Muell. Arg) is one of the varieties of cassava that contains toxic compounds cyanogenic glucosides, which cause unmarketable food products more suitable to be processed into bioethanol. This wild cassava plant produces tubers four times in weight compared to that of ordinary cassava, and the flesh contains about 40–70% starch by dry weight. This study aimed to determine the effect of the microbial types and fermentation times on bioethanol production from M. glaziovii Muell. Arg crude starch and to determine the type of microbes and fermentation time that can produce the highest bioethanol product from M. glaziovii Muell. Arg crude starch. This study consists of two factors using a factorial randomized block design (RBD). The first factor was the type of microbe which consists of 2 different microbes, namely R5I3 isolates and Saccharomyces cerevisiae ATCC 9763. The second factor was the fermentation time which consists of 4 levels, namely 3, 4, 5 and 6 days. The observed parameters were total dissolved solids value, pH value, reducing sugar content, and ethanol content. The data obtained were analyzed using analysis of variance (ANOVA) and continued with Tukey’s HSD post hoc tests. The results showed that fermentation using R5I3 isolates with a fermentation time of 5 days was the best treatment to obtain a maximum ethanol of 21.64±3.03 g/L, with a final pH value of 4.70±0.14, total dissolved solids 4.10±0.14°Brix and reducing sugar content of 0.91±0.02 g/L. Therefore, the new isolate R513 was highly potential for producing bioethanol from wild cassava crude starch.

PENGARUH KONSENTRASI RAGI Saccharomyces cerevisiae DAN LAMA FERMENTASI TERHADAP KUALITAS CUKA TOMI-TOMI (Flacourtia inermis)

2015 ◽  
Vol 4 (2) ◽  
pp. 50-55
Author(s):  
Sandra J Nendissa ◽  
Rachel Breemer ◽  
Nikholaus Melamas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Dissolved Solids ◽  
Randomized Experimental Design ◽  
Two Factors ◽  
Four Levels

This objectives of this research were both to study and determine the best level of concentration of yeast Saccharomyces cereviseae and period of fermentation on the quality of tomi-tomi vinegar (Flacourtia inermis). A completely randomized experimental design with two factors of treatment was applied in this research. The first factor was concentration of yeast S. cereviseae having four levels of tretament, i.e.: without the addition of yeast 0.5, 1 and 1.5 g yeast. The second factor was period fermentation with 1, 2, 3, 4, and 5 weeks. The result indicated that the concentration of yeast S. cereviseae 1.5 g and period fermentation 5 week produced a good tomi-tomi vinegar with total acids 51.22%, total dissolved solids 8.35, total sugar 8.07% and pH 5.40.

scholarly journals CHARACTERISTIC OF CARBONATED DRINK WITH DIFFERENT TYPE OF RHIZOMES

2020 ◽  
Keyword(s):  
Sensory Analysis ◽  
Descriptive Analysis ◽  
Total Dissolved Solids ◽  
Color Analysis ◽  
Color Intensity ◽  
Dissolved Solids ◽  
Randomized Design ◽  
Significant Difference ◽  
Different Types ◽  
Carbonated Drink

This research aimed to study the characteristics of carbonated bir pletok with different types of rhizome composition. The research design used factorial randomized design with 1 factor, it’s the type of rhizome which comprised of javanese turmeric, cutcherry, and fingerroot. Carbonated bir pletok was analysed of total dissolved solids, color and sensory analysis. The total dissolved solids of Carbonated rhizome drinks range from 5.76 to 8.13 ˚brix. Color analysis showed no significant difference (p <0.05) on the level of brightness (L *) and the level of redness (a *). While the yellowish level (b *) showed a significant difference (p> 0.05) between rhizome drinks samples (javanese turmeric, cutcherry, and finger root) to the control. Brightness level (L *) ranges from 42,236-38,182. The redness level (a *) ranges from 55,212-59,135. The yellowish level (b *) ranges from 7,6323-21,586. The result of hedonic analysis showed fingerroot bir pletok rather liked. Hedonic characteristics of fingerroot bir pletok for color intensity, ginger aroma, bitter flavor, pungent flavor, carbonation and overall of 3.80, 3.93, 3.10, 3.16. dan 3.70 respectively. The results of descriptive analysis showed the treatment of javanese turmeric, cutcherry, and fingerroot were not significantly different from the control (p <0.05) on the carbonation parameters. While the parameters of color intensity, ginger aroma, bitter flavor, pungent flavor showed significant differences (p> 0.05).

scholarly journals Potensi Susu Kedelai Asam (Soygurt) Kaya Bioaktif Peptida Sebagai Antimikroba

2017 ◽  
Vol 14 (3) ◽  
Author(s):  
Dwi Eva Nirmagustina ◽  
Chandra Utami Wirawati
Keyword(s):  
Bioactive Compounds ◽  
Block Design ◽  
Total Dissolved Solids ◽  
Total Acid ◽  
Fermentation Time ◽  
E Coli ◽  
Error Estimators ◽  
Inhibition Effect ◽  
Randomized Block Design ◽  
Number Of Cells

The research objective was to get the starter type and the fermentation time to produce soygurt that have a high peptide bioactive that functions as an antimicrobial. The study was conducted in a complete randomized block design with two replicates. The first factor is the starter types, which consists of 3 levels, 2 market starter (cimory and king yogurt) and 1 mixed starter. The second factor is the fermentation times, which consists of 3 levels, 9 hours, 12 hours, and 15 hours. The data obtained were analyzed by analysis of variance to get various error estimators and tests of significance by Duncan's test further to determine whether there is a difference between treatments. Two market starter (cimory and king yogurt) and 1 mixed starter did not produce peptide bioactive compounds . It is suspected microbes contained in the market starter cimory (S.thermophilus and L.delbrueckii ssp . Bulgaricus ), the market starter king yogurt (L.bulgaricus) and mixed starter (S.thermophilus and L.bulgaricus) did not produce peptide bioactive compounds. The inhibition effect of the test bacteria (E. coli and S. aureus) occurs due to the production of lactic acid and hydrogen peroxide during the fermentation process . The greatest inhibition shown by soygurt of the market starter cimory with a reduced number of cells in both types of test bacteria (E. coli and S. aureus) at 6 and 5 log cycles . Mixed starter has the lowest pH and the highest total acid than the marker starter cimory and king yoghurt. The starter types and the fermentation times were no significant effect on total dissolved solids of soygurt. The levels protein of soygurt, respectively 1.38 %, 1.48 %, and 1.68 (cimory, king yogurt, and mixed starter), while the fat content of soygurt 1.04%, 1.28 %, and 1.58% (cimory, mixed starter, king yoghurt) Keyword: soygurt, peptide bioactive, antimicrobe

scholarly journals PENGARUH SUHU DAN JENIS ASAM PADA HIDROLISIS PATI UBI TALAS (Colocasia esculenta L. Schott) TERHADAP KARAKTERISTIK GLUKOSA

2018 ◽  
Vol 6 (4) ◽  
pp. 307
Author(s):  
Ni Kadek Ariani Dewi ◽  
Amna Hartiati ◽  
Bambang Admadi Harsojuwono
Keyword(s):  
Block Design ◽  
Sugar Content ◽  
Total Dissolved Solids ◽  
Temperature Treatment ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Effect Of Temperature ◽  
Dissolved Solids ◽  
Randomized Block Design ◽  
Acid Type

The purpose of this reseach was 1) to determine the effect of temperature and acid type on hydrolysis of taro yam starch on the characteristics of glucose, 2) to determine the exact temperature and type of acid in taro starch hydrolysis which produced the best glucose characteristics. This reseach uses Randomized Block Design of factorial pattern.. The first factor is the hydrolysis temperature of taro yam starch at temperatures of 80°C, 90°C and 100°C. The second factor is the acids type of HNO3, H2SO4 and HCl, each with 7% acid concentration. Each treatment is grouped into 2 based on the implementation time. The variables observed were reduction in sugar content, total sugar, dekstrose equivalent (DE), total dissolved solids and clarity. The results showed that the temperature treatment and acid type had a very significant effect (P<0,01) on reducing sugar content, total sugar and hydrolyzed dectrose equivalent (DE) values. Temperature treatment has a significant effect on total dissolved solids and does not significantly affect clarity. The treatment of acid types has significant effect on total dissolved solids and clarity. Temperature treatment and type of acid interact with reducing sugar content, total sugar, and DE values, do not interact with total dissolved solids and clarity. The highest yield was obtained by reducing sugar content of 3,06%, total sugar 5,64% and DE value of 54,24% in HCl at 100°C. The total dissolved solids in H2SO4 and HCl at 100°C were 5,15°Brix and the clarity of HCl at 100°C was 0,02. The best glucose characteristics at HCl was 90°C which was not significantly different from HCl at 100°C. Keywords: sugar, hydrolysis, taro yam, starch, acid, glucose

scholarly journals Investigation on Suitable Spat Collectiors and Culture Sites for Edible Oyster in the Coast of Bay of Bengal, Bangladesh

2021 ◽  
Vol 49 (2) ◽  
pp. 257-265
Author(s):  
Jakia Hasan ◽  
Md Mozzammel Hoque ◽  
Ahmed Fazley Rabbi ◽  
Shafiqur Rahman ◽  
Md Zulfikar Ali
Keyword(s):  
Bay Of Bengal ◽  
Suspended Solids ◽  
Total Suspended Solids ◽  
Oyster Shell ◽  
Total Dissolved Solids ◽  
Dissolved Solids ◽  
Oyster Culture ◽  
Significant Difference ◽  
Edible Oyster ◽  
Suitable Location

Potential spat collectors and suitable culture sites were investigated for the development of edible oyster culture from June to December 2019 at three study sites (Moheshkhali, the Jeti of Nuniarchora, and Sonadia) of Cox’s Bazar coast of Bangladesh. Four types of collectors such as oyster shell, earthen pot, kortal (windowpane oyster shell) and tiles were investigated. On site sampling of water quality parameters and number of spats on each collector were done at 15-days interval following standard methods. Results indicated insignificant difference (P > 0.05) in temperature, salinity, dissolved oxygen and pH, while significant difference (P < 0.05) in total dissolved solids, total suspended solids and Chl-a content of the water among the study locations. However, these parameters were in suitable range for growth and spawning of oyster. Results also indicated spat recruitment was significantly influenced by the types of collectors and study locations with significantly (P < 0.05) higher spat were recruited in Tiles. Tiles were also found to facilitate with an advantage of recycling of this collector. The location in interaction with polluting materials such as total dissolved solids and total suspended solids had a significant effect on spat recruitment rate and therefore, Sonadia was the most suitable location for spat recruitment. This study site can also be used as a suitable location for the development of oyster culture in the cost of Bay of Bengal, Bangladesh based on its environmental characteristics. Bangladesh J. Zool. 49 (2): 257-265, 2021

scholarly journals KARAKTERISTIK SARI BUAH SALAK VARIETAS NANGKA (Salacca zalacca Var. ambonesnsis) PADA PENAMBAHAN JENIS DAN KONSENTRASI PENSTABIL

2019 ◽  
Vol 7 (1) ◽  
pp. 98
Author(s):  
Yohannes Eko Putra Simanullang ◽  
Ida Bagus Wayan Gunam ◽  
Ni Made Wartini
Keyword(s):  
Block Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Total Acid ◽  
Dissolved Solids ◽  
Arabic Gum ◽  
Main Research ◽  
Randomized Block Design ◽  
Stabilizer Concentration ◽  
Two Factors

The purpose of this study is to determine the type and concentration of stabilizers used in the functional drinks of salak juice and determine the type and concentration of stabilizers to produce the best characteristic salak juice. The main research aims to determine the type and concentration of stabilizers that product of salak juice. Primary research carried out by using a randomized block design (RBD) consisting of two factors The stabilizers used in this reseach that CMC, arabic gum, and gelatin. Concentration of the stabilizers used was 0.10%, 0.15%, 0.20% and 0.25%. The results showed that the type of stabilizers, concentration of stabilizers and interaction affected on the characteristics of functional fruit salak juice. The type and stabilizer concentration had an effect on total dissolved solids, viscosity, total sugar, stability, color scoring test, taste scoring test, aroma scoring test, and overall acceptance, but did not affect pH and total acid. The CMC stabilizer with a concentration of 0.25% is the best treatment for producing salak juice with characteristics of pH 4.12, total dissolved solids 13.60oBrix, total acid 0.58%, viscosity 32.0cP, total sugar 2.45%, stability with absorbance 0.580A, the color scoring rate was 3.85 (transparan yellow-pale yellow), the scoring rate was 3.80 (normal-specific), the level of aroma was 3.70 (normal-specific) and the level of overall accepted was 3.70 (normal – like). Keywords : Salak  juice, Stabilizers, Concentration , CMC, gelatin, gum arab

scholarly journals Overcoming the failure of fruit-set and fruit drop of siam orange on off-season period through application of mycorrhizal inoculants and ZNSO4 micro fertilizer dosage

2019 ◽  
Vol 3 (3) ◽  
pp. 16-24
Author(s):  
Ni Komang Alit Astiari ◽  
Ni Putu Anom Sulistiawati ◽  
Ida Bagus Komang Mahardika ◽  
I Nyoman Rai
Keyword(s):  
Fruit Set ◽  
Block Design ◽  
Total Dissolved Solids ◽  
Dissolved Solids ◽  
Fruit Drop ◽  
Randomized Block Design

This research was carried out at Kintamani District, Bangli Regency, Bali-Indonesia, from February to October 2019 with aimed to overcome the failure of fruit-set and fruit drop of Siam orange on off-season period through application of mycorrhizal inoculants and ZnSO4 micro fertilizer dosage. This experiment was arranged as a randomized block design consisted of 2 factors.  The first factor was mycorrhizal inoculant (0, 50, 100 and 150 g/tree), while the second factor was the dosage of ZnSO4 micro fertilizer (0, 5, 10 g/tree). All treatments were repeated 3 times. The results showed that the interaction between mycorrhizal inoculant treatment and ZnSO4 micro fertilizer dosage did not significantly affect all of the variables observed.  Mycorrhizal inoculant dose 150 g/tree could overcome the failure of fruit-set and fruit drop and improved the quantity and quality of fruit of Siam oranges on off-season, which was reflected by increased number of fruits harvest per tree (249,00 fruits), weight per fruit (106,10 g), weight of fruit harvested per tree (26,34 kg) and total dissolved solids (13.52% Brix), or increase 31,44%; 18,15%; 54,58%; and 35,74%, respectively, compared to without mycorrhizal inoculant i.e 189,44 fruit; 17,04 kg; 89,80 g and 9,96% Brix.

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