scholarly journals Molecular detection of Enterocytozoon bieneusi in farm-raised pigs in Hainan Province, China: infection rates, genotype distributions, and zoonotic potential

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 12 ◽  
Author(s):  
Huan-Huan Zhou ◽  
Xin-Li Zheng ◽  
Tian-Ming Ma ◽  
Meng Qi ◽  
Jing-Guo Zhou ◽  
...  
Keyword(s):  
Its Region ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Human Infection ◽  
Hainan Province ◽  
Nested Polymerase Chain Reaction ◽  
High Prevalence ◽  
Stool Samples ◽  
Group 10 ◽  
High Degree

Enterocytozoon bieneusi is a zoonotic fungal pathogen with a high degree of host diversity that can parasitize many animals, including humans. Pigs may play an important role in the epidemiology of E. bieneusi as reservoir hosts. Nevertheless, the genotypes of E. bieneusi in pigs in China remain poorly understood. The aim of this study was to determine the prevalence of E. bieneusi infection amongst pigs raised on farms from four cities of Hainan Province, using nested polymerase chain reaction (PCR) of the partial small subunit of the ribosomal RNA gene, and to identify genotypes of E. bieneusi isolates based on sequence analysis of the ribosomal internal transcribed spacer (ITS) region. Among 188 stool samples, E. bieneusi was detected in 46.8% (88/188). Eight genotypes including four known (EbpA, CS-4, MJ14, and CHG19) and four novel (HNP-I – HNP-IV) genotypes were identified. Using phylogenetic analysis, genotypes EbpA, CS4, CHG19, HNP-III, and HNP-IV were clustered into zoonotic Group 1, while the remaining three genotypes (MJ14, HNP-I, and HNP-II) clustered into Group 10. The high prevalence of zoonotic genotypes of E. bieneusi among pigs suggests that pig farming is a potential source of human infection. Additionally, this is the first identification of genotypes in Group 10 in pigs indicating unique epidemic features of E. bieneusi in pigs in Hainan Province, the southernmost part of China.

scholarly journals Genotype identification and phylogenetic analysis of Enterocytozoon bieneusi in farmed black goats (Capra hircus) from China’s Hainan Province

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 62 ◽  
Author(s):  
Huan-Huan Zhou ◽  
Xin-Li Zheng ◽  
Tian-Ming Ma ◽  
Meng Qi ◽  
Zong-Xi Cao ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Close Contact ◽  
Its Region ◽  
Enterocytozoon Bieneusi ◽  
Capra Hircus ◽  
Hainan Province ◽  
Minimal Risk ◽  
High Prevalence ◽  
Its Gene ◽  
Group 2

Enterocytozoon bieneusi is an important pathogen commonly found in humans and animals. Farmed animals with close contact to humans are important hosts of E. bieneusi. The role of goats in the transmission of E. bieneusi, however, remains unclear. In this study, 341 fresh fecal samples of black goats were collected from five locations in Hainan Province, China. Enterocytozoon bieneusi was identified and genotyped by sequences of the internal transcribed spacer (ITS) region. Phylogenetic analysis was performed by constructing a neighbor-joining tree of the ITS gene sequences. The average prevalence of E. bieneusi in black goats was 24.0% (82/341) with rates ranging from 6.3% (4/63) to 37.2% (32/86) across the locations (χ2 = 17.252, p < 0.01). Eight genotypes of E. bieneusi were identified, including six known genotypes: CHG5 (n = 47); CHG3 (n = 23); CHG2 (n = 4); CM21 (n = 3); D (n = 2); and AHG1 (n = 1), and two novel genotypes termed HNG-I (n = 1) and HNG-II (n = 1). In the phylogenetic tree, genotype D was clustered into Group 1 and the other identified genotypes were included in Group 2. This represents the first report identifying E. bieneusi in black goats from Hainan Province, with a high prevalence and wide occurrence demonstrated. The two new genotypes identified provide additional insights into the genotypic variations in E. bieneusi. Due to the small percentage of zoonotic genotypes in these animals, there is minimal risk of zoonotic transmission of E. bieneusi.

scholarly journals Prevalence of microsporidiosis in human and cattle

2017 ◽  
Vol 40 (2) ◽  
pp. 147-154
Author(s):  
Kadum N. E.
Keyword(s):  
Rural Areas ◽  
Common Species ◽  
Enterocytozoon Bieneusi ◽  
Human Infection ◽  
Urine Samples ◽  
Milk Samples ◽  
Encephalitozoon Intestinalis ◽  
Blue Stain ◽  
Stool Samples ◽  
Apparently Healthy

     In order to identify microsporidia and other fungi  in  stool and  urine samples of human, and in fecal and milk samples of cattle, 100 stool samples with or without diarrhea and 50 urine samples, human fecal and urine samples were obtained from certain Baghdad hospitals and certain rural areas surroundings Baghdad city, in addition to 50 fecal and 56  milk samples  of cattle apparently healthy were collected  from Alshula Slaughter House and  directly from  anal of the animal field  of College of Veterinary Medicine/ Baghdad University. All samples were collected during six months from 1/10/2014 to 1/4/2015. Thin films were formed and stained by Webers Modified Trichrom stain and Modified Trichrom-Ryan Blue stain. The results showed that (23%) 23 out of 100 stool samples of human were positive for Microsporidia spp. and (16%) 8 out of 50 urine samples of human were positive for this fungus. While the result revealed (18%) 9 out of 50 fecal samples and (7.14%) 4 out of 56 milk samples of cattle were positive for Microsporidia spp. The result also explained that (25.3%) 19 cases of patients suffering from diarrhea expressed   Microsporidia spp. after the examination of 75 stool samples, while (16%) 4 persons without diarrhea showed positive Microspordia, through the examination of 25 stool samples. The study explains that the  Enterocytozoon bieneusi is a common species associated with human infection and  Encephalitozoon intestinalis is  a common Microsporidia associated with  cattle infection  whereas Encephalitozoon cuniculi is rarely identified in human  but recorded in cattle.

scholarly journals Molecular-Based Detection of Enterocytozoon bieneusi in Farmed Masked Palm Civets (Paguma larvata) in Hainan, China: A High-Prevalence, Specificity, and Zoonotic Potential of ITS Genotypes

2021 ◽  
Vol 8 ◽  
Author(s):  
Wei Zhao ◽  
Guang-Xu Ren ◽  
Yu Qiang ◽  
Jiaqi Li ◽  
Jinkang Pu ◽  
...  
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Its Region ◽  
Hainan Island ◽  
Enterocytozoon Bieneusi ◽  
Zoonotic Potential ◽  
Infection Rates ◽  
Group 12 ◽  
High Prevalence ◽  
Group 1

Enterocytozoon bieneusi is a microsporidian and zoonotic species. This study investigated the prevalence and distribution of E. bieneusi genotypes in farmed masked palm civets using nested PCR, as well as assessed their zoonotic potential by phylogenetic analysis of the ITS region of the rRNA region. Here, we collected 251 fecal specimens from farmed masked palm civets (Paguma larvata) from the Hainan Island, China. In total, 128 of 251 samples were positive for E. bieneusi, with an average infection rate of 51.0%. Seventeen genotypes were identified including 12 known genotypes—HNR-VI (n = 56), SHR1 (n = 45), SHW7 (n = 6), KIN-1 (n = 3), D (n = 3), New1 (n = 3), EbpC (n = 2), CHC5 (n = 1), CHG19 (n = 1), CHN4 (n = 1), EbpA (n = 1), and Henan-III (n = 1)—and five novel genotypes (HNPL-I to HNPL-II; one each). Phylogenetic analysis categorized these genotypes into two groups. Thirteen of them were members of the zoonotic group 1, and the remaining four genotypes were in group 12. This study has shown that the infection rates of E. bieneusi in masked palm civets from Hainan were relatively high and provide baseline data to control and prevent microsporidiosis in farm-related communities. Therefore, infections in masked palm civets with zoonotic genotypes D, EbpC, CHN4, EbpA, KIN-1, and Henan-III should be considered potential threats to public health.

scholarly journals “Enterocytozoon bieneusi and Cryptosporidium: a cross-sectional study conducted throughout Thailand”

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Rapeepun Prasertbun ◽  
Hirotake Mori ◽  
Yaowalark Sukthana ◽  
Supaluk Popruk ◽  
Teera Kusolsuk ◽  
...  
Keyword(s):  
Rural Areas ◽  
Its Region ◽  
Epidemiological Data ◽  
Cross Sectional Study ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Intestinal Protozoa ◽  
Cross Sectional ◽  
Epidemiological Characteristics

Abstract Background Enterocytozoon bieneusi and Cryptosporidium spp. are prevalent zoonotic parasites associated with a high burden among children. To date only limited molecular epidemiological data on E. bieneusi and Cryptosporidium spp. in humans living in Thailand has been published. Methods PCR-based tools were used to detect and characterize E. bieneusi and Cryptosporidium spp. The internal transcribed spacer (ITS) region of the rRNA gene was used to investigate E. bieneusi, and the small subunit (SSU) rRNA gene was used to investigate Cryptosporidium spp., and 697 fecal samples from villagers and school children in rural areas in Thailand were analyzed. Results The infection rates were 2.15% (15/697) for E. bieneusi and 0.14% (1/697) for Cryptosporidium spp. The prevalence of E. bieneusi was significantly high in Loei province. Sequence analysis indicated that the Cryptosporidium isolate was C. parvum. Nine E. bieneusi genotypes were identified, EbpC, Peru12, TMH6, TMH3, TMH7, H, D, and two novel genotypes TMLH1 and TMLH2. E. bieneusi prevalence was significantly higher in male participants than in female participants, and in children aged 3–15 years than in participants aged > 15 years. Conclusions The prevalence, genotypes, and zoonotic potential of E. bieneusi were found to vary significantly high even in one country. Transmission routes and key animal carriers of E. bieneusi may be associated with differences in hygiene, sanitation, and cultural behaviors. Further molecular studies including longitudinal studies will be required to unveil epidemiological characteristics of these opportunistic intestinal protozoa in all over the countries.

scholarly journals Molecular diversity of Giardia duodenalis, Cryptosporidium spp. and Blastocystis sp. in asymptomatic school children in Leganés, Madrid (Spain)

2019 ◽  
Author(s):  
Aly Salimo Muadica ◽  
Pamela C Köster ◽  
Alejandro Dashti ◽  
Begoña Bailo ◽  
Marta Heernández de Mingo ◽  
...  
Keyword(s):  
Molecular Diversity ◽  
Parasite Species ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Published Data ◽  
Cryptosporidium Hominis ◽  
Additional Sequence ◽  
Stool Samples

Abstract Background: The protozoa Giardia duodenalis, Cryptosporidium spp., the stramenopile Blastocystis sp. and the microsporidia Enterocytozoon bieneusi are among the most frequent diarrheal pathogens affecting humans globally. This molecular epidemiological study assesses the frequency and molecular diversity of these intestinal parasites in schoolchildren in central Spain, complementing previously published data on risk and protective factors associated with parasite infection (Reh et al., Euro Surveill. 2019;24).Methods: Stool samples were collected from voluntary asymptomatic schoolchildren (4‒14 years) and their siblings (1‒16 years) attending 12 primary and secondary schools in Leganés (Madrid). Initial detection of pathogens was conducted by PCR-based methods targeting the small subunit (ssu) ribosomal RNA or the internal transcribed spacer (ITS) genes of these parasite species. Genotyping of G. duodenalis-positive samples was carried out by PCR and Sanger sequencing of appropriate markers including the glutamate dehydrogenase (gdh), the ß-giardin (bg), and triose phosphate isomerase (tpi) loci. For C. hominis/C. parvum-positive samples the 60-kDa glycoprotein (gp60) locus was used.Results: A total of 1,512 stool samples were analysed. Giardia duodenalis was the most prevalent pathogen (17.4%, 95% CI: 15.5‒19.4%), followed by Blastocystis sp. (13.0%, 95% CI: 11.4‒14.8%), and Cryptosporidium spp. (0.9%, 95% CI: 0.5%‒1.5%). Enterocytozoon bieneusi was not detected. Sequence analyses of the 24 G. duodenalis isolates genotyped at the gdh, bg, and/or tpi loci revealed the presence of sub-assemblages AII (16.6%, 4/24) and BIV (79.2%, 19/24). An additional sequence (4.2%, 1/24) represented an ambiguous BIII/BIV result. Analyses of the 14 Cryptosporidium sequences generated at the ssu rRNA allowed the identification of C. hominis (71.4%; 10/14) and C. parvum (21.4%; 3/14). An additional sequence (7.2%, 1/14) was only identified at the genus level. A total of 162 Blastocystis sp. isolates were successfully genotyped, revealing the presence of five subtypes including ST1 (22.8%; 37/162), ST2 (36.4%; 59/162), ST3 (21.6%; 35/162), ST4 (18.6%; 30/162), and ST8 (0.6%; 1/162).Conclusions: Giardia duodenalis sub-assemblage BIV, Cryptosporidium hominis and Blastocystis ST2 were the genetic variants of these parasite species more prevalent in the asymptomatic schoolchildren population investigated. These findings are very similar to those previously reported in clinical, symptomatic populations in Spain. Enterocytozoon bieneusi was absent in apparently healthy schoolchildren.

scholarly journals Title Prevalence of Bartonella Species in Shelter Cats and their Ectoparasites in Southeastern Brazil

2021 ◽  
Author(s):  
Juliana M. Raimundo ◽  
Andresa Guimarães ◽  
Gleice M. Amaro ◽  
Aline T. da Silva ◽  
Caio Junior B. C. Rodrigues ◽  
...  
Keyword(s):  
Its Region ◽  
Human Infection ◽  
Ctenocephalides Felis ◽  
Southeastern Brazil ◽  
Human Pathogens ◽  
Bartonella Species ◽  
Blood Samples ◽  
High Prevalence ◽  
Cat Fleas

Abstract Background: Feline Bartonella can be transmitted to humans through cat scratches or bites, and between cats by Ctenocephalides felis flea. Methods: The occurrence of Bartonella DNA was assessed in ectoparasites and their cat hosts living in shelters based on the ITS region and gltA gene. Results: Bartonella DNA was detected in 47.8% of cat blood samples, in 18.3% of C. felis fleas,13.3% of flea eggs pools and 12.5% of lice pools. B.henselae and B. clarridgeiae DNA were detected in cat fleas, while B. henselae, B. clarridgeiae, and B. koehlerae in blood samples from bacteremic cats. Cats infested by positive ectoparasites showed approximately twice the chance of being infected. Conclusions: Our results indicate shelter cats have a high prevalence of Bartonella species known to be human pathogens and highlight the importance of controlling their infestation by ectoparasites to avoid cat and human infection.

scholarly journals High Prevalence of Enterocytozoon bieneusi in Asymptomatic Pigs and Assessment of Zoonotic Risk at the Genotype Level

2014 ◽  
Vol 80 (12) ◽  
pp. 3699-3707 ◽  
Author(s):  
Wei Zhao ◽  
Weizhe Zhang ◽  
Fengkun Yang ◽  
Jianping Cao ◽  
Hua Liu ◽  
...  
Keyword(s):  
Opportunistic Pathogen ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Content Type ◽  
Zoonotic Risk ◽  
High Prevalence ◽  
Life Threatening ◽  
Clinically Significant

ABSTRACTEnterocytozoon bieneusiis an emerging and clinically significant enteric parasite infecting humans and animals and can cause life-threatening diarrhea in immunocompromised people. Pigs are considered to be one of the main reservoir hosts ofE. bieneusibased on their high prevalence rates and zoonotic genotypes in pigs. As an opportunistic pathogen,E. bieneusiinfection of pigs can be inapparent, which leads to neglect in detecting this parasite in pigs and assessing the epidemiological role of pigs in the transmission of human microsporidiosis. In the present study, 95 healthy pigs aged 2 or 3 months were randomly selected from three areas in Heilongjiang Province, China.E. bieneusiisolates were identified and genotyped based on the small-subunit (SSU) rRNA and internal transcribed spacer (ITS) regions of the rRNA gene by PCR and sequencing. A high prevalence ofE. bieneusiwas observed, 83.2% (79/95) at the SSU rRNA locus versus 89.5% (85/95) at the ITS locus. Ten ITS genotypes were obtained, comprising six known genotypes—EbpA (n= 30), D (n= 19), H (n= 18), O (n= 11), CS-1 (n= 1), and LW1 (n= 1)—and four novel genotypes named HLJ-I to HLJ-IV; 70.6% (60/85) ofE. bieneusigenotypes were zoonotic (genotypes EbpA, D, and O). The findings of a high prevalence ofE. bieneusiin pigs and a large percentage of zoonotic genotypes indicate that pigs may play a role in the transmission ofE. bieneusito humans and may become an important source of water contamination in our investigated areas.

scholarly journals Molecular epidemiology and genotype/subtype distribution of Blastocystis sp., Enterocytozoon bieneusi, and Encephalitozoon spp. in livestock: concern for emerging zoonotic infections

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hanieh Mohammad Rahimi ◽  
Hamed Mirjalali ◽  
Mohammad Reza Zali
Keyword(s):  
Enterocytozoon Bieneusi ◽  
Parasitic Infections ◽  
Zoonotic Potential ◽  
Transmission Dynamic ◽  
Intestinal Protozoa ◽  
Intestinal Parasitic Infections ◽  
Zoonotic Infections ◽  
High Prevalence ◽  
Blastocystis Sp ◽  
Stool Samples

AbstractIntestinal parasitic infections have high prevalence rate in many regions especially in developing countries. The aim of this study was to determine the presence and genotype/subtype of some intestinal protozoa in livestock in Iran. Stool samples were collected from cattle, sheep, chickens, and horses. The presence of targeted parasites was evaluated using real-time PCR. Genotyping/subtyping of positive samples was characterized using sequencing of the ITS and barcoding region, respectively. Blastocystis sp., 27.7% (48/173) and Enterocytozoon bieneusi 26.0% (45/173) were the most frequent protozoa followed by Encephalitozoon spp., 0.57% (1/173). Cryptosporidium spp. were not detected among samples. Encephalitozoon spp., was detected only in chickens 2.2% (1/45). A statistically correlation was seen between animals and the prevalence of targeted protozoa. E. bieneusi genotypes I (9/38; 23.68%), BEB6 (22/38; 57.89%), D (6/38; 15.79%), and horse1 (1/38; 2.63%) were detected among samples. A statistically significant correlation was seen between the genotypes and animals (P ≤ 0.05). Blastocystis sp., ST1 (1/45; 2.22%), ST5 3/45; 6.66%), ST7 (1/45; 2.22%), ST10 (24/45; 53.33%), and ST14 (16/45; 35.55%) were characterized among samples. There was no significant correlation between certain subtypes and animals (P = 0.173). The presence of zoonotic potential genotypes of E. bieneusi in animals and zoonotic potential subtypes ST1 and ST7 among our samples provide a clue about the transmission dynamic of E. bieneusi and Blastocystis sp. between animals–animals and humans–animals.

High prevalence of Enterocytozoon bieneusi in swine with four genotypes that differ from those identified in humans

Parasitology ◽  
1999 ◽  
Vol 118 (5) ◽  
pp. 447-453 ◽  
Author(s):  
A. C. BREITENMOSER ◽  
A. MATHIS ◽  
E. BÜRGI ◽  
R. WEBER ◽  
P. DEPLAZES
Keyword(s):  
Internal Transcribed Spacer ◽  
Enterocytozoon Bieneusi ◽  
Chronic Diarrhoea ◽  
Its Sequence ◽  
Red Foxes ◽  
Faecal Samples ◽  
High Prevalence ◽  
Stool Samples ◽  
Common Parasite

The microsporidial species Enterocytozoon bieneusi is found among immunocompromised, particularly HIV-infected, patients with chronic diarrhoea, and rarely also among immunocompetent persons with self-limited diarrhoea. Only recently, E. bieneusi was detected in 4 pigs in Switzerland raising the question of a potential zoonotic nature of this parasite. We examined faecal samples of 109 pigs, 24 cows, horses and red foxes each for the presence of E. bieneusi by PCR and compared these isolates with isolates obtained from stool samples of 13 HIV-infected patients living in Switzerland. In animals, E. bieneusi was only identified in pigs with a prevalence of 35%. Analysis of the rDNA internal transcribed spacer (ITS) sequence allowed the classification of E. bieneusi from 28 pigs into 4 distinct genotypes which grouped very closely (identity 96·3–98·8%) together with 2 of the 3 human-derived E. bieneusi genotypes. Hence, E. bieneusi seems to be a common parasite in swine, but no genotypes were identified that were found in humans. Nevertheless, swine might serve as a new animal model for enterocytozoonosis.

ANTICYTOKINE ACTIVITY OF THE PROTOZOA BLASTOCYSTIS SPP

2020 ◽  
pp. 44-48
Author(s):  
Bugero N.V. ◽  
Ilyina N.A. ◽  
Aleksandrova S.M.
Keyword(s):  
Gastrointestinal Tract ◽  
Gastrointestinal Diseases ◽  
Control Group ◽  
Ulcer Disease ◽  
Cytokine Balance ◽  
High Prevalence ◽  
Blastocystis Spp ◽  
High Level ◽  
Eukaryotic Organisms ◽  
High Degree

In addition to the classical pathogens, which are well understood and well identified, new pathogens with the potential to spread epidemiologically are being identified. Some of these little-known organisms are the simplest Blastocystis spp. blastocystostosis. The clinical significance of Blastocystis spp. and its pathogenicity are still under discussion. This parasite belongs to a group of single-celled eukaryotic organisms living in the colon of the human intestine. Blastocystis spp. is known to be found both in people with reduced immune status and in individuals without any clinical manifestation. It has been established that a sufficiently high degree of invasiveness is observed in persons with gastrointestinal tract diseases, dermatosis, allergic reactions, in patients with carriers of the human immunodeficiency virus, etc. Possessing persistence factors, protozoa blastocysts contribute to the inactivation of host defensive mechanisms, providing a stable anthogonistic effect. In recent years, many works have been devoted to the characteristics of the persistent properties of Blastocystis spr., however, individual properties of blastocysts, in particular, anticytokine activity (ACA), have not yet been studied. In this regard, the work studied the anticytokine activity of microorganisms isolated from healthy subjects and patients with gastrointestinal tract diseases. A high prevalence of the studied characteristic in the subjects was shown. The expression of anticytokine activity in the obtained isolates of blastocysts was the highest in the group of persons with gastric ulcer disease, which decreased in the order of duodenal ulcer, chronic cholecystitis, chronic gastritis, etc. The data obtained in this work on the high level of ACA expression in blastocyst isolates obtained from individuals with gastrointestinal diseases as compared with the control group enables to conclude that their exometabolites may influence the local cytokine balance [1], which supports the inflammatory process.

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