Cytotoxic, Antioxidant, and Antidiabetic Activities versus UPLC-ESI-QTOF-MS Chemical-Profile Analysis of Ipomoea aquatica Fractions

Planta Medica ◽  
2021 ◽  
Author(s):  
Mahmoud Hefny Gad ◽  
Kristiaan Demeyer ◽  
Yvan Vander Heyden ◽  
Debby Mangelings
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Profile Analysis ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Bioactive Substances ◽  
Ipomoea Aquatica ◽  
Antioxidant Agent ◽  
Salicylic Acid Glucoside

Abstract Ipomoea aquatica is a common green leafy vegetable that has numerous uses in traditional medicine. This study focused on the determination of the cytotoxic, antiradical, and antidiabetic properties of various fractions of the I. aquatica methanolic extract, as well as on the tentative identification of some bioactive compounds in the same fractions. The cytotoxicity was determined by the brine shrimp lethal test. The antioxidant activities of the I. aquatica fractions were investigated through 3 assays. The antidiabetic activity (in vitro) was measured by α-glucosidase and α-amylase inhibition assays. Phytochemical qualitative analyses demonstrated the presence of alkaloids, terpenoids, phenols, and flavonoids in the ethyl acetate-methanol and methanol fractions. The total phenolic and total flavonoid contents were found to be highest in the ethyl acetate-MeOH fractions. The evaluation of the cytotoxicity showed that the hexane-dichloromethane fraction is the most toxic, while the others are moderately toxic. The antioxidant activity assays showed that the ethyl acetate-MeOH fractions are the most potent, while the α-glucosidase and α-amylase assays revealed that the hexane-dichloromethane fraction might contain a potent antidiabetic agent. Some bioactive substances in the MeOH fractions, such as salicylic acid glucoside, 1-O-sinapoyl-β-D-glucose derivative, and dihydroferulic acid derivative, were tentatively identified. To the best of our knowledge, this is the first report to detect and identify these compounds in this species. Based on the results of this study, it may be concluded that I. aquatica is a potent antioxidant agent and could be a good candidate as a natural antioxidant in food and therapeutics.

scholarly journals Phenolic content and antioxidant activity of Phymatopteris hastata

2011 ◽  
Vol 76 (11) ◽  
pp. 1485-1496 ◽  
Author(s):  
Wei Su ◽  
Peiyuan Li ◽  
Lini Huo ◽  
Caiying Wu ◽  
Nana Guo ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Alcohol Extract

Various solvent extracts of Phymatopteris hastata, a traditional Chinese medicinal material, were screened for their antioxidant activities. Four systems of in vitro testing were employed to investigate the antiradical and antioxidant effect, i.e., the 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) systems, the hydroxyl radical scavenging assay and the reducing power. In addition, butylated hydroxytoluene (BHT), a widely used synthetic antioxidant, was also studied for comparison. The results revealed that the ethyl acetate extract exhibited outstanding antioxidant activities, which was close or even superior to BHT. Furthermore, the total phenolic (TP) and total flavonoid (TF) contents of different extracts were measured, expressed as gallic acid and rutin equivalent, respectively. The antioxidant activities and the TP/TF content of different extracts followed the same order: ethyl acetate extract > butyl alcohol extract > petroleum ether extract, showing a good correlation between the antioxidant activities and the TP/TF content. The results showed that these extracts, especially the ethyl acetate extract, were rich in phenolics and flavonoids and could be considered as natural antioxidants.

scholarly journals Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica

2011 ◽  
Vol 76 (5) ◽  
pp. 709-717 ◽  
Author(s):  
Peiyuan Li ◽  
Lini Huo ◽  
Wei Su ◽  
Rumei Lu ◽  
Chaocheng Deng ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soxhlet Extraction ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
In Vitro Test ◽  
Ethyl Acetate Extracts

Pouzolzia zeylanica was extracted with different solvents (acetone, ethyl acetate and petroleum ether), using different protocols (cold-extraction and Soxhlet extraction). To evaluate the antiradical and antioxidant abilities of the extracts, four in vitro test systems were employed, i.e., DPPH, ABTS and hydroxyl radical scavenging assays and a reducing power assay. All extracts exhibited outstanding antioxidant activities that were superior to that of butylated hydroxytoluene. The ethyl acetate extracts exhibited the most significant antioxidant activities, and cold-extraction under stirring seemed to be the more efficacious method for acquiring the predominant antioxidants. Furthermore, the antioxidant activities and total phenolic (TP) content of different extracts followed the same order, i.e., there is a good correlation between antioxidant activities and TP content. The results showed that these extracts, especially the ethyl acetate extracts, could be considered as natural antioxidants and may be useful for curing diseases arising from oxidative deterioration.

scholarly journals Antioxidant activities of methanolic extract and its fractions of Baccaurea racemosa and Macaranga subpeltata leaves

Food Research ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 127-134 ◽  
Author(s):  
H. Widodo ◽  
Sismindari ◽  
W. Asmara ◽  
Abdul Rohman
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Food Supplement ◽  
Positive Control ◽  
The Body ◽  
Total Phenolic ◽  
Vitro Assay ◽  
Water Fraction

Oxidative stress, the excessive presence of reactive oxygen species (ROS), is suggested as a basal cause of aging as well as various degenerative and chronic diseases in human. Antioxidants are believed to play a very vital role in the body defense system against ROS. Plant-based antioxidants with their prominence have gained tremendous worldwide interest nowadays. Baccaurea racemosa and Macaraanga subpeltata are among ethnomedical used plants for liver diseases medication which have potential source as natural antioxidants. The aim of the study was to evaluate the antioxidant activities of the methanolic crude extract (CE) and their fractions of the plant’s leaves. Maceration was performed to obtain CE, which then subjected to fractionation using n-hexane, dichloromethane, chloroform, ethyl acetate, and ethanol to obtain fractions of hexane fraction (HF), dichloromethane (DF), chloroform (CF), ethyl acetate (EAF), and ethanol fractions (EF), respectively. The CE and all fractions included water fraction (WF) and residue (R) were examined for its total phenolic contents, total flavonoid contents, and antioxidant activities using various in vitro assay. In general, EAF demonstrated as the best solvent for the extracting phenolic compounds with higher antioxidant activity. The CE and its fractions of M. subpeltata contained higher of TPC and TFC, also demonstrated higher antioxidant capacity, than that B. racemosa. The phenolics compounds were responsible for the antiradical properties. The EAF of M. subpeltata was scavenging those radicals better than that of L-(+)ascorbic acid as a positive control. The high antioxidant activities and phenolics contents make both the plant extracts to be developed as a food supplement.

scholarly journals In-Vitro Screenings for Biological and Antioxidant Activities of Water Extract from Theobroma cacao L. Pod Husk: Potential Utilization in Foods

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6915
Author(s):  
Mustanir Yahya ◽  
Binawati Ginting ◽  
Nurdin Saidi
Keyword(s):  
Ethyl Acetate ◽  
Theobroma Cacao ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Artemia Salina ◽  
Total Phenolic ◽  
Cocoa Pod Husk ◽  
Gas Chromatography Mass Spectroscopy ◽  
Theobroma Cacao L

Increasing production of cocoa (Theobroma cacao L.) leads to a higher environmental burden due to its solid waste generation. Cocoa pod husk, one of the major solid wastes of cocoa production, contains rich bioactive compounds unveiling its valorization potential. With that in mind, our research aimed to explore the biological and antioxidant activities of aqueous extracts from cocoa pod husks. In this present work, cocoa pod husk was extracted using water and subsequentially partitioned using n-hexane, ethyl acetate, and methanol. The antimicrobial investigation revealed that the ethyl acetate solubles were active against the Staphylococcus aureus, Escherichia coli, and Candida albicans, where at a 20% w/v concentration, the inhibition diameters were 6.62 ± 0.10, 6.52 ± 0.02, and 11.72 ± 0.36 mm, respectively. The extracts were found non-toxic proven by brine shrimp lethality tests against Artemia salina with LC50 scores ranging from 74.1 to 19,054.6 μg/mL. The total phenolic content and total flavonoid content were obtained in the range of 47.44 to 570.44 mg/g GAE and 1.96 to 4.34 mg/g QE, respectively. Antioxidant activities of the obtained extracts were revealed by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay with EC50 reached as low as 9.61 μg/mL by the ethyl acetate soluble. Phytochemical screening based on gas chromatography—mass spectroscopy analysis on the sample with the highest antioxidant activities revealed the dominant presence of three phytosterols, namely gamma-sitosterol, stigmasterol, and campesterol.

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

scholarly journals Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum

2021 ◽  
Vol 72 (2) ◽  
pp. 38-49
Author(s):  
Bilal Aslam ◽  
Asif Hussain
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Nigella Sativa ◽  
Piper Nigrum ◽  
Total Phenolic ◽  
Gymnema Sylvestre ◽  
Plant Materials ◽  
Methanol Extracts

This study was aimed to phytochemically characterize and assess the antioxidant activities of 70% methanol extract and subsequent fractions of Cassia absus (L.) seeds, Gymnema sylvestre (L.) whole plant, Nigella sativa (L.) seeds, and Piper nigrum (L.) seeds. Powdered plant materials were extracted with 70% methanol and further fractionated with ethyl-acetate, n-butanol and the residual aqueous fraction. Phytochemical analysis was performed to detect different phytocompounds. Mineral compositions were quantified, and total phenolic and flavonoid contents were determined. The antioxidant potential of methanol extracts and fractions was assessed in vitro through estimating DPPH radical and superoxide anion scavenging activities and reducing power assay. Extraction yields ranging highest of N. sativa methanol extract (30.42�1.49%) and lowest of P. nigrum ethyl-acetate fraction (4.58�0.61%) were obtained. Results revealed that methanol extracts and fractions of selected plants contain phytocompounds such as alkaloids, flavonoids, phenols, glycosides, tannins, terpenoids, saponins, carbohydrates, fats and fixed oils. The mineral analysis showed considerable quantities of calcium (C. absus methanol extract: 372.454�3.633 mg/100g), magnesium (G. sylvestre methanol extract: 131.045�1.346 mg/100g), and zinc (N. sativa methanol extract: 36.019�0.284 mg/100 g) in all fractions while minor quantities of manganese, copper and cobalt were also found. Methanol extracts showed considerably higher total phenolic (N. sativa methanol extract: 179.71�2.14 mg GAE/g) and flavonoid (N. sativa methanol extract: 189.18�3.17 mg CE/g) contents compared to other fractions, and subsequently exhibited pronounced scavenging activities on DPPH* (N. sativa methanol extract: 23.8 �g/mL) and superoxide radicals (N. sativa methanol extract: 24.9 �g/mL) and had potent reductive abilities (N. sativa methanol extract: 1.123�0.038 O.D.). Conclusively, C. absus, G. sylvestre, N. sativa and P. nigrum possess significant nutritive properties and could be used as natural antioxidant sources to prevent oxidative stress-associated diseases.

scholarly journals Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Imtiaz Mustafa ◽  
Muhammad Naeem Faisal ◽  
Ghulam Hussain ◽  
Humaira Muzaffar ◽  
Muhammad Imran ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Close Association ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Components ◽  
Euphorbia Helioscopia

Abstract Background Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities. Methods The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH● assay, FRAP and ABTS assays. The antidiabetic potential of the extracts was evaluated by measuring the inhibition ability of the activity of enzymes α amylase and α glucosidase. Results After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH● (IC50 value = 0.06 ± 0.02 mg/ml), FRAP (758.9 ± 25.1 μMFe+ 2/g), and ABTS (689 ± 25.94 μMTEq/g) due to the presence of high TPC (24.77 ± 0.35 mgGAEq/g) and TFC (17.95 ± 0.32 mgQEq/g) values. Antidiabetic activity in terms of inhibition potential of α amylase and α glucosidase activity was also observed maximum in methanolic extract having lowest IC50 value (0.4 ± 0.01 mg/ml and 0.45 ± 0.01 mg/ml respectively) and minimum in the aqueous extract (IC50 value = 0.57 ± 0.02 mg/ml and 0.76 ± 0.1 mg/ml respectively). Conclusion The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes.

scholarly journals Pengukuran Total Fenolik, Flavonoid, Aktivitas Antioksidan dan Antidiabetes Ekstrak Etil Asetat Daun Katemas (Euphorbia heterophylla, L.) Secara In Vitro dan In Silico Melalui Inhibisi Enzim α-Glukosidase

2020 ◽  
Vol 16 (2) ◽  
pp. 240
Author(s):  
Rahmiwati Hilma ◽  
Netti Gustina ◽  
Jufrizal Syahri
Keyword(s):  
Antioxidant Activity ◽  
Molecular Docking ◽  
Ethyl Acetate ◽  
In Silico ◽  
Ethyl Acetate Extract ◽  
Dry Weight ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Discovery Studio

<p class="Default">Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antioksidan dan antidiabetes ekstrak etil asetat daun katemas (Euphorbia heterophylla L.) secara in vitro dan in silico melalui inhibisi terhadap enzim α-glukosidase. Pada penelitian ini ekstraksi sampel dilakukan menggunakan maserasi bertingkat, dimulai dengan n-heksana, selanjutnya dengan etil asetat. Ekstrak etil asetat yang didapatkan dilakukan pengujian kuantitiatif total fenolik dan flavonoid. Uji aktivitas antioksidan terhadap ekstrak dilakukan menggunakan metode DPPH. Uji aktivitas antidiabetes terhadap ekstrak dilakukan secara in vitro dan in silico melalui inhibisi terhadap enzim α-glukosidase menggunakan akarbose sebagai standar. Uji aktivitas antidiabetes terhadap kandungan senyawa bioaktif ekstrak secara in silico atau molecular docking menggunakan software Discovery Studio 4.1. Hasil penelitian menunjukkan bahwa nilai total fenolik dari ekstrak adalah 4,24 mg GAE/g berat kering dan nilai flavonoid total adalah: 3,22 mg KE/g berat kering. Hasil uji aktivitas antioksidan ekstrak didapatkan nilai IC50 sebesar 37,56 µg/mL, digolongkan sebagai aktivitas antioksidan yang sangat kuat. Hasil uji aktivitas antidiabetes secara in vitro didapatkan nilai IC50 sebesar 138,63 µg/mL. Hasil molecular docking memperlihatkan bahwa senyawasenyawa aktif yang terdapat didalam ekstrak mampu membentuk ikatan hidrogen antara ligan dengan reseptor, tapi lebih sedikit jika dibandingkan dengan akarbose.</p><p class="Default"><strong>Measurement of Total Phenolic, Flavonoids, Antioxidant and Antidiabetic Activity of Catemas Leaf Ethyl Acetate Extract (Euphorbia heterophylla L.) by In Vitro and In Silico through Enzim α-Glucosidase Inhibition.</strong> This study aims to determine the antidiabetic activity of katemas (Euphorbia heterophylla L.) ethyl acetate extract in vitro and in silico through inhibition of the αglucosidase enzyme. In this study, the sample extraction was carried out by multilevel maceration, starting with n-hexane, then with ethyl acetate. The ethyl acetate extract obtained was quantitatively tested by total phenolic and flavonoids. The antioxidant activity of the extract was tested using the DPPH method. The antidiabetic activity of the extract was examined through inhibiting the enzyme αglucosidase in vitro using a microplate reader and in silico (molecular docking) using Discovery Studio 4.1 software. The results showed that the total phenolic value of the extract was 4.24 mg GAE/g of dry weight, and the total flavonoid value was 3.22 mg KE/g of dry weight. Antioxidant activity test obtained IC50 of 37,56 µg/mL, classified as verry strong antioxidant. The in vitro antidiabetic test examined that IC50 is 138.63 µg/mL. The results of molecular docking showed that the active compounds in the extracts are able to form hydrogen bonds between ligand and receptor; however, the amount was less than the hydrogen bonds formed by acarbose.</p>

scholarly journals Liberica Coffee (Coffea liberica L.) from Three Different Regions: In Vitro Antioxidant Activities

2021 ◽  
Vol 11 (5) ◽  
pp. 13031-13041
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Unstable Molecules ◽  
Coffea Liberica

Free radicals are unstable molecules with unpaired electrons in their outer orbitals. An antioxidant is a compound that can be scavenged free radicals. Coffee is one of the natural antioxidants. This research aimed to study the antioxidant activity of medium roasted beans of liberica coffee (Coffea liberica) from three different regions by DPPH and CUPRAC methods. To determine total phenolic content (TPC) and total flavonoid content (TFC), analyze the correlation between TPC and TFC with AAI DPPH and CUPRAC and the correlation between two methods in sample extracts. The sample was extracted by reflux using n-hexane, ethyl acetate, and ethanol. AAI DPPH in the range of 0.397- 18.536, while CUPRAC 0.532-4.674. The highest TPC in ethanol extract of liberica coffee from Aceh (22.585 ± 1.610 g GAE/100 g) and the highest TFC in ethyl acetate extract of liberica coffee from Aceh (4.927 ± 0.355 g QE/100 g). TPC of all samples had a positive and significant correlation with AAI DPPH and CUPRAC. AAI DPPH and CUPRAC value gave a significant and positive correlation. TPC of liberica coffee from three different regions contributed to antioxidant activity by DPPH dan CUPRAC methods. The two methods showed linear results in antioxidant activities of liberica coffee from three regions.

Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

2020 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Nadezhda Petkova ◽  
Manol Ognyanov ◽  
Blaga Inyutin ◽  
Petar Zhelev ◽  
Panteley Denev
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Frap Assay ◽  
Phytochemical Composition ◽  
Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

Sign in / Sign up

Export Citation Format

Share Document