Anti-cancer thymoquinone from Nigella sativa

Background: Cancer is one of the most fatal diseases across the world and it was reported that 90% of cancer fatality depends on its angiogenesis potential. Black seed or Nigella sativa L. is a medicinal plant native to southwest Asia. N. sativa has been used for medicinal purposes for centuries and predominantly has bioactive components like Thymoquinone, which is used as a candidate for anti-cancer and anti-angiogenesis drugs. Methods: Callus was induced from leaf tissue, after that alcoholic extracts were prepared from three-month-old calluses. Thymoquinone content was measured by HPLC methods. AGS cell line was cultured and treated with standard Thymoquinone and extracts from callus. Then, cell proliferation, expression of angiogenic factor (VEGF-A gene), and apoptosis test were done by MTT assay, real-time PCR and Annexin-v kit, respectively. Results: HPLC found the maximum amount of Thymoquinone in the extract of leaf calluses, which were grown in the dark. MTT assay revealed that particular doses of extracts reduced cell proliferation. Real-time and Fluorescence- Activated Cell Sorting (FACS) results demonstrated that standard Thymoquinone and callus extracts down-regulated the VEGF-A gene expression, and all three induced apoptosis in the AGS cell line. Conclusion: It has been shown that TQ has pro-apoptotic and anti-metastatic effects on stomach cancer cell line, and these properties can introduce it as an anti-cancer drug in the near future.

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Thymoquinone, an Active Compound of Nigella sativa: Role in Prevention and Treatment of Cancer

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200416092743 ◽

2020 ◽

Vol 21 (11) ◽

pp. 1028-1041 ◽

Cited By ~ 1

Author(s):

Saleh A. Almatroodi ◽

Ahmad Almatroudi ◽

Mohammed A. Alsahli ◽

Amjad A. Khan ◽

Arshad H. Rahmani

Keyword(s):

Natural Products ◽

Side Effects ◽

Cell Signaling ◽

Signaling Pathways ◽

Active Compound ◽

Nigella Sativa ◽

Cancer Drugs ◽

Normal Cells ◽

Anti Cancer ◽

Cell Signaling Pathways

Background: Cancer is the leading cause of death worldwide and the current mode of cancer treatment causes side effects on normal cells and are still the key challenges in its’ treatment. However, natural products or active compounds of medicinal plants have shown to be safe, affordable, and effective in diseases cure. Methods: In this context, scientific studies evidence the health-promoting effects of natural products, which work through its anti-oxidant, anti-inflammatory, and anti-cancer activity. Thymoquinone (TM), a predominant active compound of Nigella sativa, has confirmed anti-neoplastic activity through its ability to regulate various genetic pathways. In addition, thymoquinone has established anti-cancerous effects through killing of various cancerous cells,and inhibiting the initiation, migration, invasion, and progression of the cancer. The anti-cancer effects of TM are chiefly mediated via regulating various cell signaling pathways such as VEGF, bcl2/bax ratio, p53, NF-kB, and oncogenes. Results: The anti-cancer drugs have limitations in efficacy and also causes adverse side effects on normal cells. The combination of anti-cancer drugs and thymoquinone improves the efficacy of drugs which is evident by decrease resistance to drugs and regulation of various cell signaling pathways. Moreover, combination of anti-cancer drugs as well as thymoquinone shows synergistic effect on killing of cancer cells and cells viability. Thus, TM, in combination with anti-cancer drugs, can be a good strategy in the management of various types of cancer. Conclusion: In this review article, we deliver an outline of thymoquinone role in cancer inhibition and prevention of cancer-based on in vivo and in vitro studies. Further studies on thymoquinone based on clinical trials are highly required to explore the benefits of thymoquinone in cancer management.

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In Silico Docking Analysis and Admet Prediction of Thymoquinone Derivatives Against Ovarian Cancer

Oriental Journal Of Chemistry ◽

10.13005/ojc/370130 ◽

2021 ◽

Vol 37 (1) ◽

pp. 221-228

Author(s):

Mavara Azmi ◽

Imam Pasha Syed ◽

Anupama Koneru

Keyword(s):

Nigella Sativa ◽

Biological Activities ◽

Docking Study ◽

Pharmacokinetic Parameters ◽

Binding Pattern ◽

Active Constituent ◽

Docking Analysis ◽

In Silico Docking ◽

Anti Cancer ◽

Cancer Activity

Thymoquinone, the active constituent of Nigella sativa has been reported to have various biological activities. Due to its significance, various analogues of it have been synthesized and reported for anti-cancer activity. In the present research, we have taken the analogs of thymoquinone and performed docking study with an objective to find the binding pattern of all the molecules. Apart from this, pharmacokinetic parameters were predicted along with their toxicological parameters. From the results, the molecule Thy09 was found to have the optimized structure and further modification on this could lead to more potent compounds.

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Medicinal Herbs Used in Traditional Management of Breast Cancer: Mechanisms of Action

Medicines ◽

10.3390/medicines7080047 ◽

2020 ◽

Vol 7 (8) ◽

pp. 47

Author(s):

Donovan A. McGrowder ◽

Fabian G. Miller ◽

Chukwuemeka R. Nwokocha ◽

Melisa S. Anderson ◽

Cameil Wilson-Clarke ◽

...

Keyword(s):

Breast Cancer ◽

Nigella Sativa ◽

Curcuma Longa ◽

Mechanisms Of Action ◽

Biologically Active ◽

Black Cohosh ◽

Cochrane Library ◽

Active Components ◽

Chemotherapy Drugs ◽

Anti Cancer

Background: Breast cancer is one of the principal causes of death among women and there is a pressing need to develop novel and effective anti-cancer agents. Natural plant products have shown promising results as anti-cancer agents. Their effectiveness is reported as decreased toxicity in usage, along with safety and less recurrent resistances compared with hormonal targeting anti-cancer agents. Methods: A literature search was conducted for all English-language literature published prior to June 2020. The search was conducted using electronic databases, including PubMed, Embase, Web of Science, and Cochrane Library. The search strategy included keywords such as breast cancer, herbs, anti-cancer biologically active components, clinical research, chemotherapy drugs amongst others. Results: The literature provides documented evidence of the chemo-preventative and chemotherapeutic properties of Ginseng, garlic (Allium sativum), Black cohosh (Actaea racemose), Tumeric (Curcuma longa), Camellia sinenis (green tea), Echinacea, Arctium (burdock), Flaxseed (Linum usitatissimum) and Black Cumin (Nigella sativa). Conclusions: The nine herbs displayed anti-cancer properties and their outcomes and mechanisms of action include inhibition of cell proliferation, angiogenesis and apoptosis as well as modulation of key intracellular pathways. However, more clinical trials and cohort human studies should be conducted to provide key evidence of their medical benefits.

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Antitumoral Effects of Curcumin (Curcuma longa L.) and Thymoquinone (Nigella sativa L.) on Neuroblastoma Cell Lines

Complementary Medicine Research ◽

10.1159/000509765 ◽

2020 ◽

pp. 1-5

Author(s):

Johanna Maria Korff ◽

Katrin Menke ◽

Melanie Schwermer ◽

Katharina Falke ◽

Alexander Schramm ◽

...

Keyword(s):

Cell Lines ◽

Nigella Sativa ◽

Curcuma Longa ◽

Neuroblastoma Cell ◽

Primary Human Fibroblast ◽

Cytotoxic Effects ◽

Anti Cancer ◽

Ic50 Values ◽

Neuroblastoma Cell Lines

Introduction: Overall survival of high-risk neuroblastoma patients is still poor, emphasizing the need for novel therapeutic options. There is evidence for anti-cancer properties of the herbal substances thymoquinone and curcumin. These substances are isolated from Nigella sativa L. and Curcuma longa L., respectively, which are used in traditional medicine. Objective: We investigated cytotoxic effects of thymoquinone and curcumin on neuroblastoma cell lines NLF, NB69, and SK-N-BE(2), in vitro. Methods: Cytotoxicity of compounds was investigated by MTT cell viability assays. For analyzing effects on cell proliferation BrdU assays were employed and induction of apoptosis was detected by Cell Death ELISA assays. Results: Both substances showed cytotoxic effects in all three neuroblastoma cell lines, whereby primary human fibroblast cells reacted less sensitively. Overall, lower IC50 values could be calculated for curcumin (3.75–7.42 µM) than for thymoquinone (5.16–16.3 µM). Decreased proliferation and increased apoptosis rates were observed under treatment. Conclusions: Both substances showed anti-tumoral properties on neuroblastoma cell lines and should be further investigated as therapeutic agents.

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Uji Potensi Daya Hambat Ekstrak Metanol Biji Jintan Hitam (Nigella Sativa L.) Terhadap Bakteri Streptococcus

Jurnal Surya Medika ◽

10.33084/jsm.v6i1.1615 ◽

2020 ◽

Vol 6 (1) ◽

pp. 18-21

Author(s):

Guntur Satrio Pratomo ◽

Nurul Chusna ◽

Muhammad Priyadi

Keyword(s):

Seed Oil ◽

Methanol Extract ◽

Nigella Sativa ◽

High Concentration ◽

Black Cumin ◽

Black Seed ◽

Anti Cancer ◽

Black Cumin Seed ◽

Streptococcus Mutants ◽

Cumin Seed

One of the plants that are often used as medicinal plants is Black Seed, Black Seed is a medicinal plant known in Indonesia by the name of black cumin. Black cumin seeds and essential oils (Nigella sativa L.) have been widely used in traditional medicine. Many studies have proven the effects of Nigella sativa extract. In various studies, black cumin seed oil shows efficacy as an anti-cancer, anti-free radical and immunomodulator, analgesic, antimicrobial, anti-inflammatory, a spasmolytic, bronchodilator, hepatoprotective, and anti-hypertensive Advanced chemical analysis found that Black Seed contains carotene that is converted into vitamin A by heart. The purpose of this study was to determine the potential inhibition of methanol extract of black cumin seeds (Nigella Sativa L.) against Streptococcus. This research uses a quantitative approach with an experimental method. This research was conducted in the Pharmacognition and Microbiology Laboratory of the Faculty of Health Sciences, Muhammadiyah University, Palangkaraya. The sample used was a sample of plant material, namely Black Cumin Seed (Nigella sativa L) which had been dried and pollinated and then extracted and then tested against bacteria. The results of testing the antibacterial activity showed that from low concentration to high concentration the diameter of the resulting inhibition showed that the activity could inhibit the bacteria streptococcus mutants. The highest inhibition at a concentration of 6% is 0.83 mm and the lowest concentration at a concentration of 3% against Streptococcus mutants.

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Therapeutic Potential of Natural Products in Treatment of Cervical Cancer: A Review

Nutrients ◽

10.3390/nu13010154 ◽

2021 ◽

Vol 13 (1) ◽

pp. 154

Author(s):

Seung-Hyeok Park ◽

Minsun Kim ◽

Somi Lee ◽

Woojin Jung ◽

Bonglee Kim

Keyword(s):

Cervical Cancer ◽

Natural Products ◽

Therapeutic Potential ◽

Nigella Sativa ◽

Cassia Fistula ◽

Common Cancer ◽

Apoptosis Inhibition ◽

Anti Cancer ◽

Inhibition Of Angiogenesis ◽

Ethanol Extracts

Cervical cancer is the fourth most common cancer among women worldwide. Though several natural products have been reported regarding their efficacies against cervical cancer, there has been no review article that categorized them according to their anti-cancer mechanisms. In this study, anti-cancerous natural products against cervical cancer were collected using Pubmed (including Medline) and google scholar, published within three years. Their mechanisms were categorized as induction of apoptosis, inhibition of angiogenesis, inhibition of metastasis, reduction of resistance, and regulation of miRNAs. A total of 64 natural products suppressed cervical cancer. Among them, Penicillium sclerotiorum extracts from Cassia fistula L., ethanol extracts from Bauhinia variegate candida, thymoquinone obtained from Nigella sativa, lipid-soluble extracts of Pinellia pedatisecta Schott., and 1′S-1′-acetoxychavicol extracted from Alpinia conchigera have been shown to have multi-effects against cervical cancer. In conclusion, natural products could be attractive candidates for novel anti-cancer drugs.

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