Cytotoxicity Evaluation of Self Adhesive Composite Resin Cements by Dentin Barrier Test on 3D Pulp Cellss

ABSTRACTObjectives: The aim of this study was to evaluate the effects of five self-etch dental composite resin cements on the cell viability of bovine dental papilla-derived cells.Methods: The cytotoxicity of composite resin cements (Rely X Unicem Clicker, 3M ESPE; MaxCem; KERR, PanaviaF 2.0; Kuraray, BisCem; Bisco and Bistite II DC; Tokuyama) was analyzed in a dentin barrier test device using three-dimensional (3D) pulp cell cultures. A commercially available cell culture perfusion chamber was separated into two compartments by 500 im dentin disc. The three dimensional cultures placed on a dentin disk held in place by a special biocompatible stainless-steel holder. Test materials were introduced into the upper compartment in direct contact with the cavity side of the dentin disks according to the manufacturer’s instructions. Subsequently, the pulpal part of the perfusion chamber containing the cell cultures was perfused with medium (2 ml/h). After an exposure period of 24 h, the cell survival was determined by the MTT assay. Statistical analyses were performed using the Mann–Whitney U-test.Results: In dentin barrier test, cell survival was similar with Maxcem and negative control group (P>.05), and all other tested materials were cytotoxic for the three dimensional cell cultures (P>.05).Conclusions: The significance of composite resin cements is being more important in dentistry. The cytotoxic potencies demonstrated by these materials might be of clinical relevance. Some composite resin cements include biologically active ingredients and may modify pulp cell metabolism when the materials are used in deep cavities or directly contact pulp tissue. (Eur J Dent 2009;3:120-126)

Download Full-text

Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells

BioMed Research International ◽

10.1155/2013/910459 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Hayriye Esra Ülker ◽

Mustafa Ülker ◽

Hasan Önder Gümüş ◽

Muhammet Yalçın ◽

Abdulkadir Şengün

Keyword(s):

Cell Survival ◽

Dental Pulp ◽

Three Dimensional ◽

T Antigen ◽

Biologically Active ◽

Luting Cements ◽

Cytotoxicity Testing ◽

Barrier Test ◽

Cytotoxicity Studies ◽

Dentin Barrier Test

This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P<0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P>0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.

Download Full-text

Scanning Electron Microscopy and Electron Microprobe Analysis of Malignant Cell Cultures

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100561 ◽

1968 ◽

Vol 26 ◽

pp. 164-165

Author(s):

R. I. Johnsson-Hegyeli ◽

A. F. Hegyeli ◽

D. K. Landstrom ◽

W. C. Lane

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Cell Cultures ◽

Electron Microprobe ◽

Microprobe Analysis ◽

Electron Microprobe Analysis ◽

Three Dimensional ◽

Malignant Cell ◽

Interference Microscopy ◽

Scanning Electron

Last year we reported on the use of reflected light interference microscopy (RLIM) for the direct color photography of the surfaces of living normal and malignant cell cultures without the use of replicas, fixatives, or stains. The surface topography of living cells was found to follow underlying cellular structures such as nuceloli, nuclear membranes, and cytoplasmic organelles, making possible the study of their three-dimensional relationships in time. The technique makes possible the direct examination of cells grown on opaque as well as transparent surfaces. The successful in situ electron microprobe analysis of the elemental composition and distribution within single tissue culture cells was also reported.This paper deals with the parallel and combined use of scanning electron microscopy (SEM) and the two previous techniques in a study of living and fixed cancer cells. All three studies can be carried out consecutively on the same experimental specimens without disturbing the cells or their structural relationships to each other and the surface on which they are grown. KB carcinoma cells were grown on glass coverslips in closed Leighto tubes as previously described. The cultures were photographed alive by means of RLIM, then fixed with a fixative modified from Sabatini, et al (1963).

Download Full-text

Characterization of photosystem II with the atomic-force microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130365 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1146-1147

Author(s):

Kathleen M. Marr ◽

Mary K. Lyon

Keyword(s):

Photosystem Ii ◽

Atomic Force Microscope ◽

Three Dimensional ◽

Biologically Active ◽

Atomic Force ◽

Freeze Etching ◽

Image Averaging ◽

Oxygen Evolving ◽

Averaging Techniques

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.

Download Full-text

Modifiers of the metabolism of biologically active substances of the cell cultures of Silybum marianum L. of Belarus and Hungarian breeding

Fiziologia rastenij i genetika ◽

10.15407/frg2019.06.493 ◽

2019 ◽

Vol 51 (6) ◽

pp. 493-507

Author(s):

O.V. Kovzunova ◽

◽

V.N. Reshetnikov ◽

Keyword(s):

Cell Cultures ◽

Biologically Active Substances ◽

Biologically Active ◽

Silybum Marianum ◽

Active Substances

Download Full-text

An Update on Recent Advances in Cubosome: A Novel Drug Delivery System

Current Drug Metabolism ◽

10.2174/1389200221666210105121532 ◽

2021 ◽

Vol 21 ◽

Author(s):

Madhukar Garg ◽

Anju Goyal ◽

Sapna Kumari

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Lipid Bilayers ◽

Delivery System ◽

Liquid Crystalline ◽

Three Dimensional ◽

Biologically Active ◽

Potential Applications ◽

Health Related ◽

Novel Drug

: Cubosomes are highly stable nanostructured liquid crystalline dosage delivery form derived from amphiphilic lipids and polymer-based stabilizers converting it in a form of effective biocompatible carrier for the drug delivery. The delivery form comprised of bicontinuous lipid bilayers arranged in three dimensional honeycombs like structure provided with two internal aqueous channels for incorporation of number of biologically active ingredients. In contrast liposomes they provide large surface area for incorporation of different types of ingredients. Due to the distinct advantages of biocompatibility and thermodynamic stability, cubosomes have remained the first preference as method of choice in the sustained release, controlled release and targeted release dosage forms as new drug delivery system for the better release of the drugs. As lot of advancement in the new form of dosage form has bring the novel avenues in drug delivery mechanisms so it was matter of worth to compile the latest updates on the various aspects of mentioned therapeutic delivery system including its structure, routes of applications along with the potential applications to encapsulate variety drugs to serve health related benefits.

Download Full-text

Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽

10.1515/med-2020-0031 ◽

2020 ◽

Vol 15 (1) ◽

pp. 249-253

Author(s):

Marta Bochynska-Czyz ◽

Patrycja Redkiewicz ◽

Hanna Kozlowska ◽

Joanna Matalinska ◽

Marek Konop ◽

...

Keyword(s):

Cell Cultures ◽

Chemical Activation ◽

Three Dimensional ◽

Enzymatic Digestion ◽

Pepsin Digestion ◽

Cell Culturing ◽

3D Cell Cultures ◽

Associated Proteins ◽

Positive Effect ◽

Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

Download Full-text