The nature of the perennial response in Mediterranean grasses. II. Senescence, summer dormancy, and survival in Phalaris

1968 ◽  
Vol 19 (3) ◽  
pp. 397 ◽  
Author(s):  
JR McWilliam

Adaptation to summer drought has been one of the major factors influencing the life cycle of the Mediterranean grasses. The nature of this adaptation has been studied by comparing the behaviour of a typical perennial, Phalaris tuberosa, with a closely related annual species P. minor, under simulated summer drought conditions. One of the most important factors controlling the plant survival of these two species is the difference in the rate and pattern of senescence after flowering under the influence of moisture stress. In the annual, senescence is rapid and complete, but in the perennial it is retarded, and function is retained in certain organs, including the lower stem and root system, throughout the summer stress. This permits survival of dormant buds which contain the meristems vital for regrowth in the autumn. Another feature which may be important in the perennial is the limited carbon assimilation by the dormant culms during the summer which contributes a source of energy for the maintenance of root activity, and for the regeneration of dormant buds. The evidence suggests that these factors may be of general significance, and could well form the basis of the perennial response in other Mediterranean grass species.


2016 ◽  
Vol 38 (5) ◽  
pp. 501 ◽  
Author(s):  
M. L. Mitchell ◽  
J. M. Virgona ◽  
J. L. Jacobs ◽  
D. R. Kemp

Microlaena stipoides (microlaena) is an important perennial grass in over 7 million hectares of native pastures in southern Australia and can survive and persist despite severe soil water deficits during summer. Many other pasture species survive similar conditions by relying on summer dormancy, which raises the possibility that microlaena may behave similarly. A field experiment using rainout shelters was conducted on an existing microlaena pasture in north-east Victoria. The experiment was a split-plot design with two watering treatments (‘summer storm’ or ‘summer dry’) as main plots and three defoliation treatments (nil, intense defoliation, strategic defoliation) as subplots. The ‘summer storm’ treatment resulted in the formation of new buds and tillers and increased basal cover from 1% in February to 18% in March. A glasshouse pot experiment examined the recovery of microlaena after different periods of drought and subsequent rewatering. In the pot experiment, microlaena withstood relatively short (up to 30 days) dry periods and then recovered when rewatered. Thus, it appears that microlaena is a persistent, perennial pasture plant that, although it survives very dry summers in Mediterranean areas, is not summer dormant. Microlaena does not exhibit summer dormancy in response to moisture stress and enter a quiescent stage, because normal growth is prevented by the lack of water, but it quickly recommences growth when soil water becomes available. The ability of microlaena to withstand summer soil water deficits and to recruit from seedlings make it a valuable pasture species across drought-prone environments, and this undoubtedly partly explains its very broad adaptation across eastern Australia.



2020 ◽  
Vol 26 (4) ◽  
pp. 397-406
Author(s):  
T. E. Chekanova

The presented study examines the problems of integration of the national banking systems of the member states of the Eurasian Economic Union (EAEU).Aim. The study aims to examine the major differences in various aspects of functioning of banking systems in the EAEU member states in terms of their impact on integration processes.Tasks. The author identifies the most prominent features of the banking systems of the EAEU states; reveals the depth of the existing differences through a comparative analysis of various indicators of national banking systems; outlines ways of overcoming integration problems associated with differences in the banking sectors of the Union states.Methods. This study is based on universal general scientific methods and elements of comparative, functional, and economic analysis within the framework of a systems approach. The author uses regulatory documents and banking reports of the EAEU states, statistical and analytical materials of the Eurasian Economic Commission (EEC), and data of Moody’s international rating agency.Results. The study identifies a number of aspects that contain the major differences in the functioning of banking systems in the EAEU member states; highlights the disproportions in the scale, level of development, financial stability, and risks of the banking spheres of the Union states; comparatively analyzes the proportion of banking and non-banking structures in the system and the share of the government and non-resident companies in the capital of banks; marks the difference in the pricing of banking services; determines differences in the existing approaches to banking regulation and the established standards; analyzes the major differences in the legislative acts of the central banks and governments of the EAEU member states and in the terms and definitions used. According to the results of the study, the major factors hindering the development of integration processes between the banking systems of the EAEU states are identified.Conclusions. The existing differences between the banking systems of the EAEU countries are diverse and multifaceted. The author states that the aspects addressed in this study have a significant negative impact on the further development of integration processes, describing the major directions and actions of the member states aimed at minimizing the exiting differences, which are required to facilitate the convergence of the states and the transition towards a common financial market.



2019 ◽  
Vol 20 (11) ◽  
pp. 1046-1051 ◽  
Author(s):  
Przemysław Gajda-Morszewski ◽  
Klaudyna Śpiewak-Wojtyła ◽  
Maria Oszajca ◽  
Małgorzata Brindell

Lactoferrin was isolated and purified for the first time over 50-years ago. Since then, extensive studies on the structure and function of this protein have been performed and the research is still being continued. In this mini-review we focus on presenting recent scientific efforts towards the elucidation of the role and therapeutic potential of lactoferrin saturated with iron(III) or manganese(III) ions. The difference in biological activity of metal-saturated lactoferrin vs. the unmetalated one is emphasized. The strategies for oral delivery of lactoferrin, are also reviewed, with particular attention to the metalated protein.



2021 ◽  
Vol 29 (1) ◽  
pp. 230949902199606
Author(s):  
Takeshi Mochizuki ◽  
Koichiro Yano ◽  
Katsunori Ikari ◽  
Ken Okazaki

Purpose: This study investigated the clinical effects of different patellar components without being affected by the femoral component design in total knee arthritis (TKA) for patients with knee osteoarthritis (OA). Methods: In total, 48 patients with OA who met the criteria of the American College of Rheumatology for OA were enrolled and randomly assigned in a 1:1 ratio to two groups according to the usage of patellar component design for TKA (medialized dome type [dome group] or medialized anatomic type [anatomic group]). To evaluate the clinical outcomes for TKA, knee range of motion (ROM), pain intensity of 0–100 mm visual analog scale (pain VAS), and the Japanese Knee Osteoarthritis Measure (JKOM) score were obtained at baseline and year 1. Results: The difference in knee ROM, pain VAS, or total JKOM score at year 1 was not significant between the dome and anatomic groups ( p = 0.398, 0.733 and 0.536, respectively). Moreover, similar results were obtained for changes in knee ROM, pain VAS, or total JKOM scores from baseline. In both groups, the pain VAS and total JKOM scores were significantly improved at year 1. Conclusion: Both dome and anatomic groups in TKA are significantly effective for pain and function using the JKOM score. However, their efficacy did not differ, according to the JKOM score. Results of this study are rare information focusing on the patellar component design and provide one of the insights into the TKA clinical management.



2021 ◽  
Vol 11 (9) ◽  
pp. 4048
Author(s):  
Javier A. Linares-Pastén ◽  
Lilja Björk Jonsdottir ◽  
Gudmundur O. Hreggvidsson ◽  
Olafur H. Fridjonsson ◽  
Hildegard Watzlawick ◽  
...  

The structures of glycoside hydrolase family 17 (GH17) catalytic modules from modular proteins in the ndvB loci in Pseudomonas aeruginosa (Glt1), P. putida (Glt3) and Bradyrhizobium diazoefficiens (previously B. japonicum) (Glt20) were modeled to shed light on reported differences between these homologous transglycosylases concerning substrate size, preferred cleavage site (from reducing end (Glt20: DP2 product) or non-reducing end (Glt1, Glt3: DP4 products)), branching (Glt20) and linkage formed (1,3-linkage in Glt1, Glt3 and 1,6-linkage in Glt20). Hybrid models were built and stability of the resulting TIM-barrel structures was supported by molecular dynamics simulations. Catalytic amino acids were identified by superimposition of GH17 structures, and function was verified by mutagenesis using Glt20 as template (i.e., E120 and E209). Ligand docking revealed six putative subsites (−4, −3, −2, −1, +1 and +2), and the conserved interacting residues suggest substrate binding in the same orientation in all three transglycosylases, despite release of the donor oligosaccharide product from either the reducing (Glt20) or non-reducing end (Glt1, Gl3). Subsites +1 and +2 are most conserved and the difference in release is likely due to changes in loop structures, leading to loss of hydrogen bonds in Glt20. Substrate docking in Glt20 indicate that presence of covalently bound donor in glycone subsites −4 to −1 creates space to accommodate acceptor oligosaccharide in alternative subsites in the catalytic cleft, promoting a branching point and formation of a 1,6-linkage. The minimum donor size of DP5, can be explained assuming preferred binding of DP4 substrates in subsite −4 to −1, preventing catalysis.



2001 ◽  
Vol 281 (5) ◽  
pp. H1938-H1945 ◽  
Author(s):  
Chari Y. T. Hart ◽  
John C. Burnett ◽  
Margaret M. Redfield

Anesthetic regimens commonly administered during studies that assess cardiac structure and function in mice are xylazine-ketamine (XK) and avertin (AV). While it is known that XK anesthesia produces more bradycardia in the mouse, the effects of XK and AV on cardiac function have not been compared. We anesthetized normal adult male Swiss Webster mice with XK or AV. Transthoracic echocardiography and closed-chest cardiac catheterization were performed to assess heart rate (HR), left ventricular (LV) dimensions at end diastole and end systole (LVDd and LVDs, respectively), fractional shortening (FS), LV end-diastolic pressure (LVEDP), the time constant of isovolumic relaxation (τ), and the first derivatives of LV pressure rise and fall (dP/d t max and dP/d t min, respectively). During echocardiography, HR was lower in XK than AV mice (250 ± 14 beats/min in XK vs. 453 ± 24 beats/min in AV, P < 0.05). Preload was increased in XK mice (LVDd: 4.1 ± 0.08 mm in XK vs. 3.8 ± 0.09 mm in AV, P < 0.05). FS, a load-dependent index of systolic function, was increased in XK mice (45 ± 1.2% in XK vs. 40 ± 0.8% in AV, P < 0.05). At LV catheterization, the difference in HR with AV (453 ± 24 beats/min) and XK (342 ± 30 beats/min, P < 0.05) anesthesia was more variable, and no significant differences in systolic or diastolic function were seen in the group as a whole. However, in XK mice with HR <300 beats/min, LVEDP was increased (28 ± 5 vs. 6.2 ± 2 mmHg in mice with HR >300 beats/min, P < 0.05), whereas systolic (LV dP/d t max: 4,402 ± 798 vs. 8,250 ± 415 mmHg/s in mice with HR >300 beats/min, P < 0.05) and diastolic (τ: 23 ± 2 vs. 14 ± 1 ms in mice with HR >300 beats/min, P < 0.05) function were impaired. Compared with AV, XK produces profound bradycardia with effects on loading conditions and ventricular function. The disparate findings at echocardiography and LV catheterization underscore the importance of comprehensive assessment of LV function in the mouse.



1996 ◽  
Vol 271 (2) ◽  
pp. G377-G385 ◽  
Author(s):  
D. M. Christie ◽  
P. A. Dawson ◽  
S. Thevananther ◽  
B. L. Shneider

An apical sodium-dependent bile acid transporter (ASBT) has recently been cloned and characterized in the rat ileum. Northern and Western blotting revealed both the ASBT mRNA and protein in rat kidney. The coding sequence of the kidney transcript was found to be identical to the previously cloned ileal ASBT. Indirect immunofluorescence studies localized the ASBT protein to the apical membrane of the renal proximal convoluted tubule. Kinetic analysis of sodium-dependent taurocholate uptake using membrane vesicles revealed a similar Michaelis-Menten constant value for taurocholate in the kidney and intestine. ASBT protein and function were present in the kidney but not the ileum from 7-day-old rats. On postnatal day 7, there was a sevenfold increase in ASBT steady-state mRNA levels in the kidney relative to the ileum, yet nuclear run-on assays revealed that the nascent transcription rates at this age were virtually the same. This suggests that the difference in the neonatal expression of the ASBT gene in the kidney and ileum may be in part due to differences in mRNA stability.



2017 ◽  
Vol 68 (8) ◽  
pp. 781
Author(s):  
R. A. Culvenor ◽  
M. R. Norton ◽  
J. De Faveri

Perennial grasses have production and environmental benefits in areas of southern Australia typified by the mixed farming zone of southern New South Wales (NSW). The perennial grass phalaris (Phalaris aquatica L.) is widely used in southern Australia; however, it would find more use in the mixed farming zone if its persistence in marginal rainfall areas (450–500 mm average annual rainfall) were improved. We evaluated a range of germplasm (n = 29) including wild accessions, lines bred from these, and existing cultivars for persistence and production at three sites in a summer-dry area of southern NSW with 430–460-mm average annual rainfall. Two sites were used over 4 years and the third site over 5 years. Summer dormancy, maturity time and seedling growth were also assessed. Analysis of genotype × environment interaction employing factor analytic models and accounting for spatial and temporal correlations indicated that changes in persistence occurred mainly over time rather than between sites. Ranking changes occurred in the dry establishment phase of the experiment and during a severe final summer drought, with few changes occurring in the intervening high-rainfall years. Lines that survived the establishment phase best had vigorous seedlings and earlier maturity, whereas those surviving the final summer best were earlier maturing and higher in summer dormancy with high winter-growth activity. Some later maturing lines within the higher summer dormancy group were less persistent. Some accessions from North Africa were the most persistent; also, populations bred from these and other more persistent accessions generally persisted and produced better than cultivars used presently. However, present cultivars were capable of high yield in the higher rainfall years. We suggest that persistence of higher summer dormancy cultivars over very dry years could be improved by selecting for earlier maturity time.



2018 ◽  
Vol 2 (126) ◽  
pp. 31-52
Author(s):  
Husein M. Alothman ◽  
Mohammed Al-Hourani

This study analyzes the post-divorce experiences of a sample of 50 Jordanian divorced participants. Qualitative and exploratory results are similar to the results of other studies that were conducted in certain western cultures. Similar issues included modern reasons for exiting a marriage, men's experiences of losing children and women's poor financial circumstances. Despite changes in the structure and function of the Jordanian family, post-divorce experiences continue to be influenced by the major factors of a traditional gender culture regarding the traditional reasons to exit marriage, with women retaining custody of children when they do not remarry and failure to establish cooperative custody. In addition, the results of this study reveal that progressive changes in women's choices to exit their marriages to better themselves. The study also sheds light on the perception of violence against married women as a reason for exiting marriage and family support to minimize the negative effects of divorce on women with a concurrent realization of the stigmatization of society toward divorced women. Nevertheless, additional research on this topic is needed to significantly add to our understanding of these phenomena



2020 ◽  
Vol 48 (4) ◽  
pp. 1419-1432 ◽  
Author(s):  
Edmund R.S. Kunji ◽  
Jonathan J. Ruprecht

For more than 40 years, the oligomeric state of members of the mitochondrial carrier family (SLC25) has been the subject of debate. Initially, the consensus was that they were dimeric, based on the application of a large number of different techniques. However, the structures of the mitochondrial ADP/ATP carrier, a member of the family, clearly demonstrated that its structural fold is monomeric, lacking a conserved dimerisation interface. A re-evaluation of previously published data, with the advantage of hindsight, concluded that technical errors were at the basis of the earlier dimer claims. Here, we revisit this topic, as new claims for the existence of dimers of the bovine ADP/ATP carrier have emerged using native mass spectrometry of mitochondrial membrane vesicles. However, the measured mass does not agree with previously published values, and a large number of post-translational modifications are proposed to account for the difference. Contrarily, these modifications are not observed in electron density maps of the bovine carrier. If they were present, they would interfere with the structure and function of the carrier, including inhibitor and substrate binding. Furthermore, the reported mass does not account for three tightly bound cardiolipin molecules, which are consistently observed in other studies and are important stabilising factors for the transport mechanism. The monomeric carrier has all of the required properties for a functional transporter and undergoes large conformational changes that are incompatible with a stable dimerisation interface. Thus, our view that the native mitochondrial ADP/ATP carrier exists and functions as a monomer remains unaltered.



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