Site variation in the deposition of linoleic acid in adipose tissue of cattle given formaldehyde-treated sunflower seed

1976 ◽  
Vol 27 (6) ◽  
pp. 895 ◽  
Author(s):  
RL Hood ◽  
RF Thornton

Fatty acid analyses were carried out on adipose tissues from several anatomical locations in steers raised either under grazing conditions or on a diet containing 30% of formaldehyde-treated sunflower seed. The level of linoleic acid deposited in adipose tissue of steers receiving the protected sunflower seed (PSS) varied from 9.2% in the brisket to 20.4% in the channel fat. A technique which permits the simultaneous calculation of both the extent of deposition of exogenous lipid at any anatomical location, and the degree of in vivo protection of the seed oil from ruminal hydrogenation, is described. By means of this technique, the PSS was calculated to be 71.6% protected from in vivo hydrogenation, and fat from the PSS was found to be preferentially incorporated into the internal depots and the tissues of the forequarter rather than those in the hindquarters of the steers. This technique has application in the determination of patterns of deposition of dietary fat at different stages of development and in different breeds of cattle and sheep.

2011 ◽  
Vol 347-353 ◽  
pp. 3337-3340
Author(s):  
Shu Ting Qi ◽  
Yu Xin Shi ◽  
Ming Dong ◽  
Hong Qing Wu ◽  
Di Mu

Methods currently used for the quantitative determination of total lipids and fatty acid composition in plant require solvent extraction. This study examined the solvent extraction of purple medic seed oil. Results varied with different extraction conditions. The effects of temperature, extraction time, sample size and solvent type on the extraction efficiency and oil quality were studied. The amount of extract collected was highest when ether absolute was used as a solvent for solvent extraction.


1938 ◽  
Vol 16b (5) ◽  
pp. 158-175 ◽  
Author(s):  
J. W. McCutcheon

Linoleic acid has been prepared from sunflower seed oil by a modification of Rollett's method. The melting point of the solid tetrabromostearic acid is placed at 115.2 °C. The boiling point, specific gravity, iodine value, and refractive index of the ethyl ester and the melting point of the free acid were determined. Linoleamide was prepared, and its melting point is placed at approximately 58 °C. Through rebromination of the prepared linoleic acid, the isomeric liquid tetrabromostearic acid was isolated, and is described. Its reduced acid is shown to be identical with that derived from the solid isomer. From these data and additional data from the literature, it was concluded that bromination and debromination cause no cis-trans isomerization, and that the two pairs of optically active enantiomorphous bromo derivatives exist, not because of their derivation from different acids, but rather because of the four asymmetric carbon atoms which they contain. Therefore, only one naturally occurring linoleic acid is at present known which is identical with that produced through reduction of the tetrabromide that melts at 115.2 °C. or the corresponding liquid derivative. No attempt is made to assign any particular cis-trans configuration to this acid, or to exclude the theoretical existence of three others.


1994 ◽  
Vol 72 (1) ◽  
pp. 127-145 ◽  
Author(s):  
Sandra R. Farwer ◽  
Bernardus C. J. Der Boer ◽  
Edward Haddeman ◽  
Gerardus A. A. Kivits ◽  
Antoon Wiersma ◽  
...  

Twelve groups of eight rats and two control groups of sixteen rats were given semisynthetic diets with 40% energy as fat for a period of 76 d. All diets contained a minimum of 3% energy as linoleic acid and comparable basal levels of D-α- and D-γ-tocopherol. The diets varied in fat composition and in the content of DL-α-tocopheryl acetate. The diets high in polyunsaturated fatty acids (PUFA) were either rich in fish oil (FO; groups 1–4; 10% energy as fish oil PUFA), linseed oil (LN; groups 1–4; 10% energy as α-linolenic acid) or sunflower seed oil (SF; groups 1–4; 10 + 3% energy as linoleic acid). The control groups were given a diet high in monounsaturated fatty acids (MUFA; CO 1; 10 + 13% energy as oleic acid) or a diet with an ‘average’ linoleic acid content (CO 2; 8.5% energy as linoleic acid). Of each high PUFA diet three groups were supplemented with graded levels of DL-α-tocopheryl acetate. Steatitis, a sensitive histopathological indicator of vitamin E deficiency in animals fed on diets rich in fatty acids with three or more double bonds, was observed only in the adipose tissue of the FO groups, even in the group with the highest DL-α-tocopheryl acetate supplementation. Liver and serum α- tocopherol levels were found to be positively correlated and liver and serum γ-tocopherol levels negatively correlated with dietary DL-α-tocopheryl acetate. The groups on the FO diets had significantly reduced liver and serum tocopherol levels in comparison with the groups on the other high-PUFA diets. With the supplementation scheme used for the FO groups the liver α-tocopherol levels of both control groups were reached but the serum control levels were not.


1972 ◽  
Vol 27 (1) ◽  
pp. 19-26 ◽  
Author(s):  
J. Glavind

1. The colorimetric micro-adaption of the iodometric method and the colorimetric thiocyanate method for the determination of lipoperoxides were compared. Similar results were obtained when methyl linoleate hydroperoxide was tested, but when lipid from rat liver, muscle, kindney and testes was examined, substantial amounts were found by the iodometric, but almost nothing by the thiocyanate method.2. The main reason for the discrepancy between the methods seems to be that the iodometric micromethod also estimates substances other than true lipoperoxides. The presence of ubiquinone and vitamin A in the organ extracts was shown to interfere in this way in the method.3. The yellow colour which develops when retinol and its esters are tested by the iodometric micromethod is due not to liberated iodine but to conversion products of retinol.4. It is concluded that the occurrence of substantial amounts of lipoperoxides in vivo has so far been demonstarted only in the adipose tissue, and not in the parenchymatous organs of the rat.


2011 ◽  
Vol 233-235 ◽  
pp. 2629-2632 ◽  
Author(s):  
Xiao Nian Xiao ◽  
Hai Bin Yi ◽  
Xing Yi ◽  
Hua Xiong

A simple and convenient method for simultaneous determination of two major effective components (oleic acid and linoleic acid) in Coix seed oil has been developed. The sample was analyzed in a RP-C18 column with acetonitrile: tetrahydrofuran: water (containing 0.4% HAC) (77:3:20, v/v/v) as a mobile phase, and the differential refractive index detector was used in HPLC. The column temperature was fixed at 30°C and the flow rate was 1.0 ml/min. The average recovery rate of oleic acid and linoleic acid are 102.83% and 99.33%, the RSD (n=6) are 1.24% and 2.27%, respectively. The standard curves of oleic acid and linoleic acid presented the favorable linear correlation over the range of 0.098-10.05 mg/ml and 0.101-10.38 mg/ml(R2 >0.999). The method reported in this paper is reliable and relatively simple with a good reproducibility for the determination of oleic acid and linoleic acid in Coix seed oil comparing with the existing methods.


2016 ◽  
Vol 94 (7) ◽  
pp. 2821-2834 ◽  
Author(s):  
C. Raschka ◽  
L. Ruda ◽  
P. Wenning ◽  
C.-I. von Stemm ◽  
C. Pfarrer ◽  
...  

2016 ◽  
Vol 38 (4) ◽  
Author(s):  
YANUZI MARA VARGAS CAMILO ◽  
ELI REGINA BARBOZA DE SOUZA ◽  
RONALDO VELOSO NAVES ◽  
ROSÂNGELA VERA ◽  
MUZA DO CARMO VIEIRA

ABSTRACT The present research aimed to determine the fatty acid profile in the seed oil of cagaita fruits cultivated in Goiânia, state of Goiás, in 2013 and 2014 harvests. The study was carried out with fruits produced by cagaita trees cultivated in the field of the School of Agronomy, Federal University of Goiás (EA/UFG), Goiânia, GO, where, in 1998, 440 cagaita seedlings from different municipalities in Goiás were planted. Fruits were collected from plants with 16 years of age in full production stage. Cagaita seeds show in their fatty acid profile mostly linoleic acid, oleic acid and palmitic acid. There was no variation in fatty acids content present in seeds between years 2013 and 2014, assuming that there are no external influences such as weather, foliar nutrients or soil, in the production of these oils


1985 ◽  
Vol 63 (11) ◽  
pp. 1411-1417 ◽  
Author(s):  
P. L. McLennan ◽  
M. Y. Abeywardena ◽  
J. S. Charnock

Coronary artery ligation (CAL) was used to produce an in vivo model of cardiac arrhythmias and myocardial infarction using anaesthetized male Hooded Wistar rats which had been fed for 6–7 or 18–20 months on either a standard reference diet alone or supplemented (12% w/w) with sunflower seed oil (linoleic acid rich) or sheep kidney fat (linoleic acid poor). The number of ventricular extra beats and duration of tachycardia or fibrillation in the 30-min postligation was increased in sheep kidney fat-fed rats. Infarct size 4 h postCAL was reduced in sunflower seed oil-fed rats. Arrhythmias, infarct size, and dietary-induced differences were increased with age. The diets employed produce changes in myocardial membrane phospholipids which could result in altered prostaglandin production. These results show that in the rat (as in man), age and dietary saturated fat are risk factors for sudden cardiac death and myocardial infarction and suggest that the rat is a useful model for the investigation of dietary interventions in heart disease.


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