Resistance in annual Medicago species to Phoma medicaginis under controlled environment and field conditions

1990 ◽  
Vol 30 (2) ◽  
pp. 209 ◽  
Author(s):  
MJ Barbetti
Keyword(s):  
Temperature Regime ◽  
Environmental Conditions ◽  
Field Data ◽  
Plant Age ◽  
Controlled Environment ◽  
Temperature Regimes ◽  
Phoma Medicaginis ◽  
Annual Medicago ◽  
Medicago Species ◽  
Line Resistance

Seven Medicago species, comprising 11 cultivars and 9 seed lines, were screened for resistance to Phoma black stem disease (caused by Phoma medicaginis) at 3 temperature regimes (day/night) of 15/10, 17.5/12.5 and 20/15�C and at 3 plant ages under controlled environment conditions. The same cultivars and lines were screened in the field over 2 seasons. There were large differences among species, and between cultivars and lines of any 1 species, in the severity of Phoma black stem disease on plants 1,4 and 10 weeks of age and at the 3 temperature regimes under controlled environment conditions, and in field screenings. Under controlled environmental conditions the cultivar and line resistance rankings were influenced by plant age, in particular, and also by the temperature regime. Cultivar and line resistance to P. medicaginis on 10-week-old plants under controlled environment conditions was correlated with some field results but resistance to this disease on 1- and 4-week-old plants was not correlated with field data.

Phoma medicaginis Isolate Differences Determine Disease Severity and Phytoestrogen Production in Annual Medicago spp.

Plant Disease ◽  
2021 ◽  
Author(s):  
Mahtab Omidvari ◽  
Gavin Flematti ◽  
Ming Pei You ◽  
Payman Payman Abbaszadeh-Dahaji ◽  
Martin John BARBETTI
Keyword(s):  
Disease Severity ◽  
Disease Incidence ◽  
Severe Disease ◽  
Fold Increase ◽  
Leaf Spot Disease ◽  
Leaf Disease ◽  
Phoma Medicaginis ◽  
Annual Medicago ◽  
Relationship Of ◽  
Medicago Species

Phoma black stem and leaf spot disease of annual Medicago spp., caused by Phoma medicaginis, not only can devastate forage and seed yield, but also reduces herbage quality by inducing production of phytoestrogens, particularly coumestrol and 4'-O-methylcoumestrol, that can reduce ovulation rates of animals grazing infected forage. We determined the consequent phytoestrogen levels on three different annual Medicago species/cultivars (M. truncatula cv. Cyprus, M. polymorpha var. brevispina cv. Serena and M. murex cv. Zodiac), following inoculation with 35 isolates of P. medicaginis. Across the isolate x cultivar combinations, leaf disease incidence (%LDI), petiole disease incidence (%PDI), leaf disease severity (%LDS), petiole disease severity (%PDS), and leaf yellowing severity (%LYS) ranged up to 100, 89.4, 100, 58.1 and 61.2%, respectively. Cultivars Cyprus and Serena were most susceptible and cv. Zodiac the most resistant to P. medicaginis. Isolates WAC3653, WAC3658 and WAC4252 produced the most severe disease. Levels of phytoestrogens in stems ranged from 25 to 1995 mg/kg for coumestrol and from 0 to 418 mg/kg for 4'-O-methylcoumestrol. There was a significant positive relationship of disease incidence and severity parameters with both coumestrol and 4'-O-methylcoumestrol contents, as noted across individual cultivars, and as across the three cultivars overall where r=0.39 and 0.37 for coumestrol and 4'-O-methylcoumestrol, respectively (P<0.05). Although, cv. Serena was most susceptible to P. medicaginis and produced the highest levels of phytoestrogens in the presence of P. medicaginis, cv. Zodiac contained the highest levels of phytoestrogens in comparison with other cultivars in the absence of P. medicaginis. There was a 15-fold increase in coumestrol in cv. Serena but only a 7-fold increase in cv. Zodiac from infection of P. medicaginis. The study highlights that the intrinsic ability of a particular cultivar to produce phytoestrogens in the absence of the pathogen, and its comparative ability to produce phytoestrogens in the presence of the P. medicaginis, are both important and highly relevant to developing new annual Medicago spp. cultivars that offer improved disease resistance and better animal reproductive outcomes.

Resistance in annual Medicago species to Phoma medicaginis and Leptosphaerulina trifolii under field conditions

1995 ◽  
Vol 35 (2) ◽  
pp. 209 ◽  
Author(s):  
MJ Barbetti
Keyword(s):  
Disease Resistance ◽  
Field Conditions ◽  
Breeding Lines ◽  
Disease Progress Curve ◽  
Leaf Disease ◽  
Progress Curve ◽  
Phoma Medicaginis ◽  
Annual Medicago ◽  
Medicago Species ◽  
New Cultivars

Advanced breeding lines and potential new cultivars of annual Medicago species were screened for resistance to phoma leaf and stem disease (caused by Phoma medicaginis) and pepper spot (caused by Leptosphaerulina trifolii) in the field, and their performances were compared with appropriate control cultivars. Medicago murex had the best overall disease resistance of the Medicago species tested. There were large differences between varieties in the incidence of both diseases. Based on end-of-season scores, the most resistant to phoma leaf disease included M. murex cv. Zodiac and SAR 3490, M. tornata SA10012, and M. truncatula cv. Parabinga, with scores 52.8 on a 0-10 scale (0, nil disease; 10, >90% leaves affected). On the basis of area under disease progress curve (AUDPC), the most resistant to phoma leaf disease, with AUDPC values of <157, included all M. murex varieties except 87FB2.30, and M. truncatula cv. Caliph. Based on end-of-season scores, the most resistant to phoma stem disease, all with scores c4, included M. murex cv. Zodiac, SAR 3490, and 87F01.48; M. sphaerocarpos cv. Orion (SEP 29.1), SEP 29.2, and SEP 30.3; and M. tornata cv. Tornafield and SA10012. Resistance to phoma stem disease in these lines was confirmed by AUDPC values, especially for M. murex cv. Zodiac, SAR3490, and 87FO1.48, and for M. tornata SA10012, with values <154. Based on end-of-season scores, the most resistant to pepper spot leaf disease included M. polymorpha cvv. Serena and Circle Valley, with scores 52.8. On the basis of AUDPC, all M. murex varieties except SAR3490 and 87FO1.5 could be included among those most resistant to pepper spot leaf disease, with AUDPC values <275. Coumestrol levels in stems ranged from 117 to 1420 mg/kg DW, with lowest levels (5330 mg/kg) in M. murex cv. Zodiac, 87FO 1.48, and GRC 87; M. sphaerocarpos cv. Orion (SEP 29.1), SEP 29.2, and SEP 26.2.1; M. tornata cv. Tornafield; and M. truncatula Z601. There was positive correlation between the severity of phoma disease on stems and end-of-season (September-November) coumestrol concentrations.

scholarly journals Properties of Extruded Snacks Prepared from Corn and Carrot Powder with Ascorbic Acid Addition

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1367
Author(s):  
Valentina Obradović ◽  
Jurislav Babić ◽  
Verica Dragović-Uzelac ◽  
Antun Jozinović ◽  
Đurđica Ačkar ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Temperature Regime ◽  
External Source ◽  
Single Screw Extruder ◽  
Functional Value ◽  
Temperature Regimes ◽  
Starting Point ◽  
Extruded Snacks ◽  
New Type ◽  
Corn Snacks

The objective of this research was to investigate the potentiality of carrot powder (CP) utilization at levels 4, 6, or 8% as ingredient of corn snacks and evaluation of the extrusion influence on functionally important ingredients such as carotenoids (color), polyphenols, fiber, fat, and antioxidant activity. The influence of ascorbic acid (AA) as an external source at levels 0.5 and 1% on this particular extrusion was also investigated. A single-screw extruder at two temperature regimes (135/170/170 °C (E1) and 100/150/150 °C (E2)) carried out extrusion. The E1 temperature regime acted favorably on total polyphenol content and crude fiber, but fat preferred the E2 regime. Extrusion, especially the E1 temperature regime, increased the extractability of carotenoids. Ascorbic acid degraded during extrusion, but it still provided protection to carotenoids and color attributes of extrudates. Snacks with increased nutritional and functional value due to carrot powder addition were successfully produced, which is a starting point for production of a new type of extruded snacks.

SELECTIVE CONTROL OF ANNUAL BLUEGRASS IN KENTUCKY BLUEGRASS TURF WITH LINURON

1979 ◽  
Vol 59 (2) ◽  
pp. 469-473 ◽  
Author(s):  
R. G. INGRATTA ◽  
G. R. STEPHENSON ◽  
C. M. SWITZER
Keyword(s):  
Temperature Regime ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Controlled Environment ◽  
Night Temperature ◽  
Annual Bluegrass ◽  
Selective Control ◽  
Excellent Control

Optimum top growth of annual bluegrass (Pao annua L.) and Kentucky bluegrass (Poa pratensis L.) was obtained at 24/12 °C day/night temperature regime in controlled environment studies. The tolerance of seedling Kentucky bluegrass to linuron [3-(3,4-dichlorophenyl)-1)methylurea] appeared to be greatest at this temperature regime when photoperiods were 16 h in length. A granular formulation of linuron gave excellent control of annual bluegrass in Kentucky bluegrass turf at 6.7 kg/ha when applied postemergence. At this rate, all culitivars of Kentucky bluegrass tested, with the exception of Fylking, were tolerant to linuron as a granular formulation. After application of linuron at 3.4–6.7 kg/ha, phytotoxic residues remained in the soil at sufficient levels to injure seedling Kentucky bluegrass for up to 3 mo.

scholarly journals Fungal and Bacterial Diversity Patterns of Two Diversity Levels Retrieved From a Late Decaying Fagus sylvatica Under Two Temperature Regimes

2021 ◽  
Vol 11 ◽  
Author(s):  
Sarah Muszynski ◽  
Florian Maurer ◽  
Sina Henjes ◽  
Marcus A. Horn ◽  
Matthias Noll
Keyword(s):  
Species Richness ◽  
Bacterial Community ◽  
Mass Loss ◽  
Community Composition ◽  
Fagus Sylvatica ◽  
Temperature Regime ◽  
Community Diversity ◽  
Interaction Patterns ◽  
Temperature Regimes ◽  
Natural Diversity

Environmental fluctuations are a common occurrence in an ecosystem, which have an impact on organismic diversity and associated ecosystem services. The aim of this study was to investigate how a natural and a species richness-reduced wood decaying community diversity were capable of decomposing Fagus sylvatica dead wood under a constant and a fluctuating temperature regime. Therefore, microcosms with both diversity levels (natural and species richness-reduced) were prepared and incubated for 8 weeks under both temperature regimes. Relative wood mass loss, wood pH, carbon dioxide, and methane emissions, as well as fungal and bacterial community compositions in terms of Simpson‘s diversity, richness and evenness were investigated. Community interaction patterns and co-occurrence networks were calculated. Community composition was affected by temperature regime and natural diversity caused significantly higher mass loss than richness-reduced diversity. In contrast, richness-reduced diversity increased wood pH. The bacterial community composition was less affected by richness reduction and temperature regimes than the fungal community composition. Microbial interaction patterns showed more mutual exclusions in richness-reduced compared to natural diversity as the reduction mainly reduced abundant fungal species and disintegrated previous interaction patterns. Microbial communities reassembled in richness-reduced diversity with a focus on nitrate reducing and dinitrogen-fixing bacteria as connectors in the network, indicating their high relevance to reestablish ecosystem functions. Therefore, a stochastic richness reduction was followed by functional trait based reassembly to recover previous ecosystem productivity.

scholarly journals Thermal stability analysis of particles incorporated in cirrus crystals and of non-activated particles in between the cirrus crystals: Comparing clean and polluted air masses

2003 ◽  
Vol 3 (4) ◽  
pp. 3659-3679 ◽  
Author(s):  
M. Seifert ◽  
J. Ström ◽  
R. Krejci ◽  
A. Minikin ◽  
A. Petzold ◽  
...  
Keyword(s):  
Temperature Regime ◽  
Water Soluble ◽  
Cloud Formation ◽  
Residual Fraction ◽  
Volatile Fraction ◽  
Air Masses ◽  
Temperature Regimes ◽  
Soluble Sulfate ◽  
Indirect Information ◽  
Cold Case

Abstract. A thermal volatility technique is used to provide indirect information about the chemical composition of the aerosol involved in cirrus cloud formation. The fraction of particles that disappears after being heated to 125°C is termed volatile and the fraction that disappears between 125 and 250°C is termed semi-volatile. Particles that still remain after being heated to 250°C make up the non-volatile fraction. The thermal composition of residual particles remaining from evaporated cirrus crystals is presented and compared to interstitial aerosol particles (non-activated particles in between the cirrus crystals) for two temperature regimes (cold: T<235 K, warm: 235<T<250 K), based on in-situ observations. The observations were conducted in cirrus clouds in the Southern Hemisphere (SH) and Northern Hemisphere (NH) midlatitudes during the INCA project. In the cold temperature regime, the non-volatile fraction of the residual particles was typically in the range 10 to 30% in the NH and 30 to 40% in the SH. In the warm temperature regime, the non-volatile residual fraction was typically 10 to 30% (NH) and 20 to 40% (SH). At high crystal number densities the non-volatile fraction in both temperature regimes was even higher: in the range of 30 to 40% (NH) and 40 to 50% (SH). The semi-volatile fraction was typically less than 10% in both hemispheres, causing the volatile fraction to essentially be a complement to the non-volatile fraction. In terms of the fractioning into the three types of particles, the SH cold case is clearly different compared to the other three cases (the two warm cases and the cold NH case), which share many features. In the NH data the distribution of different particle types does not seem to be temperature dependent. In all the cases, the non-volatile fraction is enriched in the residual particles compared to the fractions observed for the interstitial particles. This enrichment corresponds to about 15 (NH) and 30 (SH) percent units in the two cold cases and to 15–25 (NH) and 25–35 (SH) percent units in the two warm cases. In the NH cold case, there is a clear relation between the fractions observed in the interstitial particles and what is observed in the residual particles. The observed large fractions of non-volatile particles show that particles forming ice crystals are not entirely made up of water-soluble sulfate particles.

RESPONSE OF PEAS TO ENVIRONMENT: II. EFFECTS OF TEMPERATURE IN CONTROLLED-ENVIRONMENT CABINETS

1966 ◽  
Vol 46 (2) ◽  
pp. 195-203 ◽  
Author(s):  
B. Stanfield ◽  
D. P. Ormrod ◽  
H. F. Fletcher
Keyword(s):  
Plant Development ◽  
Dry Matter ◽  
Accumulation Rate ◽  
Stem Elongation ◽  
Controlled Environment ◽  
Dry Matter Accumulation ◽  
Average Temperature ◽  
Pisum Sativum L ◽  
Temperature Regimes ◽  
Effects Of Temperature

Effects of day/night temperature regimes from 7/4 to 32/24 °C on growth and development of Pisum sativum L. var. Dark Skin Perfection were studied in controlled-environment cabinets. Light intensity was about 1500 foot-candles and the photoperiod was 16 hours. Rate of plant development, in terms of nodes produced per day, increased steadily as the average temperature increased. Rate of stem elongation, however, was most rapid at 21/13 °C; and plant height was greatest at 16/10 °C. On a dry matter accumulation rate basis, vine growth decreased above and below a temperature optimum which shifted from 21/16 to 16/10 °C in the course of plant development. The combination of high day and high night temperatures caused an increase in the number of nodes to the first flower. Tillering was most prolific at the lower temperatures and was absent at 32 °C day temperatures. Pea yield decreased as temperature increased above 16/10 °C, due mainly to a reduction in the number of pods per plant.

INTERACTION OF TEMPERATURE AND CO2 ENRICHMENT ON SOYBEAN: GROWTH AND DRY MATTER PARTITIONING

1987 ◽  
Vol 67 (1) ◽  
pp. 59-67 ◽  
Author(s):  
NASSER SIONIT ◽  
B. R. STRAIN ◽  
E. P. FLINT
Keyword(s):  
Low Temperature ◽  
Environmental Control ◽  
Weight Ratio ◽  
Controlled Environment ◽  
Plant Responses ◽  
Growth Responses ◽  
Dry Matter Partitioning ◽  
Leaf Weight ◽  
Temperature Regimes ◽  
Increasing Temperature

Projected increases in atmospheric CO2 concentration will affect growth and productivity of many plant species under various environmental conditions. Since these increases in CO2 may also increase mean annual temperatures, it is important to determine how the soybean (Glycine max (L.) Merr.) will respond to changes in temperature regimes associated with atmospheric CO2 enrichment. Morphology and growth responses of the Ransom cultivar, which is adapted to a southern U.S.A. climate, to day/night temperature regimes of 18/12, 22/16, and 26/20 °C and atmospheric CO2 concentrations of 350, 675 and 1000 μL L−1 were studied in controlled environment chambers. Plant responses were determined at 20, 40, 67 and 115 (late senescence to maturity) days after planting. Plant height and number of branches increased slightly with CO2 enrichment and more significantly with increasing temperature. Root to shoot ratio remained unchanged at different CO2 concentrations but decreased as temperature increased. Leaf weight ratio and specific leaf weight decreased with increasing temperature. Low temperature reduced dry weight of all plant parts, but the reduction was ameliorated by increasing atmospheric CO2 concentration. The results show that increasing the atmospheric CO2 level causes soybean to grow more vigorously at low temperatures. Although controlled environment experiments have their drawbacks in regard to natural field conditions, the present data indicate that soybean will have enhanced growth even at moderately cool temperatures in the future global CO2 concentrations.Key words: Soybean growth, low temperature, CO2 × temperature interaction, environmental control

scholarly journals Assessing the role of two populations of Aedes japonicus japonicus for Zika virus transmission under a constant and a fluctuating temperature regime

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Uros Glavinic ◽  
Jasmin Varga ◽  
Anca Ioana Paslaru ◽  
Jeannine Hauri ◽  
Paul Torgerson ◽  
...  
Keyword(s):  
Constant Temperature ◽  
Temperature Regime ◽  
Zika Virus ◽  
Incubation Temperature ◽  
Transmission Rate ◽  
Control Measures ◽  
Fluctuating Temperature ◽  
Laboratory Conditions ◽  
Temperature Regimes ◽  
Two Populations

Abstract Background Since the huge epidemic of Zika virus (ZIKV) in Brazil in 2015, questions were raised to understand which mosquito species could transmit the virus. Aedes aegypti has been described as the main vector. However, other Aedes species (e.g. Ae. albopictus and Ae. japonicus) proven to be competent for other flaviviruses (e.g. West Nile, dengue and yellow fever), have been described as potential vectors for ZIKV under laboratory conditions. One of these, the Asian bush mosquito, Ae. japonicus, is widely distributed with high abundances in central-western Europe. In the present study, infection, dissemination and transmission rates of ZIKV (Dak84 strain) in two populations of Ae. japonicus from Switzerland (Zürich) and France (Steinbach, Haut-Rhin) were investigated under constant (27 °C) and fluctuating (14–27 °C, mean 23 °C) temperature regimes. Results The two populations were each able to transmit ZIKV under both temperature regimes. Infectious virus particles were detected in the saliva of females from both populations, regardless of the incubation temperature regime, from 7 days post-exposure to infectious rabbit blood. The highest amount of plaque forming units (PFU) (400/ml) were recorded 14 days post-oral infection in the Swiss population incubated at a constant temperature. No difference in terms of infection, dissemination and transmission rate were found between mosquito populations. Temperature had no effect on infection rate but the fluctuating temperature regime resulted in higher dissemination rates compared to constant temperature, regardless of the population. Finally, transmission efficiency ranged between 7–23% and 7–10% for the constant temperature and 0–10% and 3–27% under fluctuating temperatures for the Swiss and the French populations, respectively. Conclusions To the best of our knowledge, this is the first study confirming vector competence for ZIKV of Ae. japonicus originating from Switzerland and France at realistic summer temperatures under laboratory conditions. Considering the continuous spread of this species in the northern part of Europe and its adaptation at cooler temperatures, preventative control measures should be adopted to prevent possible ZIKV epidemics.

scholarly journals Differential expression of TLP, ERF1, and R2R3MYB in annual Medicago species under salinity conditions

2015 ◽  
Vol 14 (3) ◽  
pp. 10152-10164 ◽  
Author(s):  
F. Gharaghani ◽  
F. Rafiei ◽  
N. Mirakhorli ◽  
E. Ebrahimie
Keyword(s):  
Differential Expression ◽  
Annual Medicago ◽  
Medicago Species
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