scholarly journals Prediction of zinc deficiency in navy beans (Phaseolus vulgaris) by soil and plant analyses

1990 ◽  
Vol 30 (4) ◽  
pp. 557 ◽  
Author(s):  
JD Armour ◽  
AD Robson ◽  
GSP Ritchie

Navy beans (Phaseolus vulgaris cv. Gallaroy) were grown with 7 rates of zinc (Zn) in a Zn-deficient gravelly sandy loam in a glasshouse experiment. The plant shoots were harvested 31 days after sowing and the Zn concentration in each of 4 plant parts (YL, young leaf; YOL, young open leaf; YFEL, youngest fully expanded leaf; and whole shoots) was related to the fresh weight of the shoots. The critical Zn concentrations (mgtkg) in the plant parts determined by the 2 intersecting straight lines model were 21.1 for YL (r2 = 0.66), 17.1 for YOL (r2 = 0.83), 10.6 for YFEL (r2 = 0.91) and 12.5 for the whole tops (r2 = 0.88). The YFEL was selected as an appropriate diagnostic tissue because it is readily identifiable in the field and had the highest 1.2 with fresh weight. In a second glasshouse experiment, the critical Zn concentration in the YFEL and 5 soil tests were evaluated for their ability to predict the Zn status of navy beans. There were 13 soils from sands to clays with a wide range of chemical properties. The soil tests were 0.1 mol/L HCl, DTPA, EDTA, dilute CaCl2 and soil solution Zn. The concentration of Zn in the YFEL correctly predicted Zn deficiency or adequacy in about 77% of samples. The results from both experiments showed that a critical Zn concentration of 10-11 mg/kg in the YFEL can be used to diagnose the Zn status of Gallaroy navy beans. It was not possible to recommend a single soil test for prediction of the relative yield of navy beans. A combination of quantity (HCl, EDTA, DTPA) and intensity (soil solution, 0.002 mol/L CaCl2, 0.01 mol/L CaCl2) parameters were able to explain most of the variation in the Zn concentration of the YFEL, a more sensitive measure of nutrient availability than relative yield. EDTA-Zn in combination with 0.01 mol/L CaCl2-Zn explained 90% of the variation in the Zn concentration in the YFEL, while HCl- or DTPA-Zn and 0.01 mol/L CaCl2 explained about 80% of the variation. As soil solution Zn was significantly correlated with 0.002 and 0.01 mol/L CaCl2-Zn (r = 0.75, P<0.01; r = 0.62, P<0.05, respectively), CaCl2-Zn may be used as a more convenient measure of Zn intensity than soil solution Zn.

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 975
Author(s):  
Ye-Eun Park ◽  
Chang-Ha Park ◽  
Hyeon-Ji Yeo ◽  
Yong-Suk Chung ◽  
Sang-Un Park

Peanut (Arachis hypogaea) is a crop that can produce resveratrol, a compound with various biological properties, such as those that exert antioxidant, anticancer, and anti-inflammatory effects. In this study, trans-resveratrol was detected in the roots, leaves, and stems of tan and purple seed coat peanuts (Arachis hypogaea) cultivated in a growth chamber. Both cultivars showed higher levels of resveratrol in the roots than the other plant parts. Thus, both cultivars were inoculated with Agrobacterium rhizogenes, in vitro, to promote hairy root development, thereby producing enhanced levels of t-resveratrol. After 1 month of culture, hairy roots from the two cultivars showed higher levels of fresh weight than those of seedling roots. Furthermore, both cultivars contained higher t-resveratrol levels than those of their seedling roots (6.88 ± 0.21 mg/g and 28.07 ± 0.46 mg/g, respectively); however, purple seed coat peanut hairy roots contained higher t-resveratrol levels than those of tan seed coat peanut hairy roots, ranging from 70.16 to 166.76 mg/g and from 46.61 to 54.31 mg/g, respectively. The findings of this study indicate that peanut hairy roots could be a good source for t-resveratrol production due to their rapid growth, high biomass, and substantial amount of resveratrol.


1968 ◽  
Vol 46 (7) ◽  
pp. 921-928 ◽  
Author(s):  
D. J. Durzan

In late August during the onset of dormancy in spruce, seasonal levels of soluble nitrogen, rich in arginine, were high. On a fresh weight basis, diurnal levels of total soluble nitrogen and most component amino acids in roots, buds, and leaves showed maxima, one at sunrise and another in the afternoon or near sunset.Arginine and glutamine in the different plant parts contributed 44 to 83% to the alcohol-soluble nitrogen. In buds and leaves, percentage of arginine remained high and decreased slightly at midday, whereas in roots a continual drop occurred. In all organs examined, changes in glutamine reflected the double maxima of total soluble nitrogen and were greatest in roots.On a fresh weight basis, most amino acids accumulated at sunrise and near sunset; however a few especially in leaves, increased at midday, e.g. glutamic and aspartic acid, lysine, γ-aminobutyric acid, and serine.Comparison of levels of free guanidino compounds in different organs showed remarkable out-of-phase patterns. Levels of these compounds are known from 14C-arginine studies to be closely related to the metabolism of arginine.


2016 ◽  
Vol 141 (2) ◽  
pp. 196-208 ◽  
Author(s):  
Celina Gómez ◽  
Cary A. Mitchell

The relative coolness-to-touch of light-emitting diodes (LEDs) has enabled commercial implementation of intracanopy lighting (ICL) in the greenhouse. Intracanopy lighting, which refers to the strategy of lighting along the side or from within the foliar canopy, can increase canopy photosynthetic activity, but physiological and productivity responses of high-wire greenhouse tomato (Solanum lycopersicum) to intracanopy supplemental lighting (SL) still are not yet fully understood. Two consecutive production experiments were conducted across seasons in a glass-glazed greenhouse located in a midnorthern, continental climate [lat. 40°N (West Lafayette, IN)]. Plants were grown from winter-to-summer [increasing solar daily light integral (DLI)] and from summer-to-winter (decreasing solar DLI) to compare three SL strategies for high-wire tomato production across changing solar DLIs: top lighting with high-pressure sodium lamps (HPS) vs. intracanopy LED vertical towers vs. hybrid SL (HPS + horizontal ICL-LEDs). A control treatment also was included for which no SL was provided. Supplemental DLI for each experimental period was adjusted monthly, to complement seasonal changes in sunlight, aiming to approach a target total DLI of 25 mol·m‒2·d‒1 during fruit set. Harvest parameters (total fruit fresh weight, number of fruit harvested, and average cluster fresh weight), tissue temperature, chlorophyll fluorescence, and stomatal conductance (gS) were unaffected by SL treatment in both experiments. Among the physiological parameters evaluated, CO2 assimilation measured under light-saturating conditions, light-limited quantum-use efficiency, and maximum gross CO2 assimilation (Amax) proved to be good indicators of how ICL reduces the top-to-bottom decline in leaf photosynthetic activity otherwise measured with top lighting only (HPS-SL or solar). Although SL generally increased fruit yield relative to control, lack of SL treatment differences among harvest parameters indicates that higher crop photosynthetic activity did not increase fruit yield. Compared with control, intracanopy SL increased yield to the same extent as top SL, but the remaining photoassimilate from ICL most likely was partitioned to maintain nonharvested, vegetative plant parts as well.


Blood ◽  
1949 ◽  
Vol 4 (5) ◽  
pp. 670-675 ◽  
Author(s):  
JONAH G. LI ◽  
EDWIN E. OSGOOD

Abstract A method is described for rapid and efficient separation of leukocytes and nucleated erythrocytes from blood or marrow. It is based on the rediscovery of a nontoxic hemagglutinin, isolated from common red or navy beans, which agglutinates all human erythrocytes and those of the animals which have been tested with it. The time from drawing the blood to complete separation of the cells is less than ten minutes. The cells remain in suspension in their own plasma and are countable. Negligible amounts of foreign material are introduced, a great advantage in chemical studies. The cells so isolated are living and suitable for culture studies. Any volume of blood from less than 1 ml. to over 500 ml. may be processed. In marrow nucleated erythrocytes are separated with the leukocytes and in their original porportions. The volume, motility, morphology and life span of the cells in cultures are not altered by addition of the bean extract.


Weed Science ◽  
1977 ◽  
Vol 25 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Omosuyi Fadayomi ◽  
G.F. Warren

The site of uptake of nitrofen (2,4-dichlorophenyl-p-nitrophenyl ether) and oxyfluorfen [2-chloro-1-(3-ethoxy-4-nitrophenoxy)-4-(trifluoromethyl)benzene] was studied using a double pot technique. Sorghum [Sorghum bicolor(L.) Moench ‘RS610′] and pea (Pisum sativumL. ‘Alaska’) were the test plants. Herbicidal activity measured by the reduction in fresh weight of the roots and shoots of treated plants showed that exposure of the shoot zone to the herbicides caused much more injury to the plants than root exposure. Translocation of both compounds from root applications to tops of pea and sorghum was studied using14C-labeled herbicides. There was very little movement of the compounds from the roots of both species. Translocation of the compounds from foliage application was studied using greenbean (Phaseolus vulgarisL. ‘Spartan Arrow’) and soybean [Glycine max(L.) Merr ‘Wayne’]. Almost all of the applied14C-herbicides remained at the point of application.


Soil Research ◽  
1989 ◽  
Vol 27 (4) ◽  
pp. 699 ◽  
Author(s):  
JD Armour ◽  
GSP Ritchie ◽  
AD Robson

The effect of the incubation of zinc (Zn) applied to the soil on Zn uptake and the Zn concentrations in chemical extractants was studied. In a glasshouse experiment using a Zn-deficient gravelly sandy loam, the effect of recently applied Zn was compared with that of Zn incubated with the soil for 15 days at 40�C on growth and Zn uptake by navy beans (Phaseolus vulgaris cv. Gallaroy). At the second harvest (33 days after sowing), the dry weight of shoots of recently applied Zn was consistently higher than that of incubated Zn, except at the highest rate of 1 �g Zn g-1 soil, where yields were similar. Comparisons of the slope of the linear regressions of Zn uptake as a function of rate of application showed that incubated Zn was approximately 80% as effective as recently applied Zn. A laboratory experiment measured the decrease in Zn concentration in HCl, EDTA, DTPA, and dilute CaCl2 with incubation for up to 8 days at 40�C in four contrasting soils from Western Australia and Queensland. An addition of 2.5 �g Zn g-1 soil increased the concentration of Zn in all extractants at all times of incubation compared with the untreated soil. The recovery of the added Zn was generally highest with HCl and lowest with 0.002 M CaCl2 and decreased exponentially in all extractants with increasing time of incubation in all soils. The order of the rate of decrease in Zn concentration for all extractants was krasnozem > gravelly sandy loam > sand > sandy clay loam. The model, Y = CtB, where C and B are constants, was used to describe the relationship between the recovery of added Zn and time of incubation.


2015 ◽  
Vol 95 (4) ◽  
pp. 703-710 ◽  
Author(s):  
J. G. Boersma ◽  
A. Hou ◽  
C. L. Gillard ◽  
K. B. McRae ◽  
R. L. Conner

Boersma, J. G., Hou, A., Gillard, C. L., McRae, K. B. and Conner, R. L. 2015. Impact of common bacterial blight on the yield, seed weight and seed discoloration of different market classes of dry beans (Phaseolus vulgaris L.). Can. J. Plant Sci. 95: 703–710. Common bacterial blight (CBB) is a seed-borne disease of dry bean (Phaseolus vulgaris L.), causing significant economic loss to growers due to reductions in seed yield and quality and the need to annually purchase disease-free seed. Over the past decade a number of breeding lines and cultivars with resistance to CBB have been developed in several bean market classes including navy, black and cranberry beans. A comparison of three susceptible navy bean cultivars and seven resistant navy, three black and one cranberry bean entries in Manitoba revealed that most resistant navy and black bean lines had significantly reduced the incidence of leaf symptoms and their mean yield losses were reduced to less than 17%, while those of the susceptible lines were as high as 36% under severe disease pressure. Only the weakly resistant navy bean cultivar HR67 and the cranberry bean line F4GR1 failed to substantially reduce CBB symptoms or show a yield advantage. The Simple Sequence Repeat (SSR) marker PVctt001 in combination with Sequence Characterized Amplified Region (SCAR) marker SU91 was associated with a low incidence of CBB symptoms and a reduced yield loss in five navy bean lines, but not in the cranberry bean line F4GR1. Disease symptoms on the pods in the resistant black and navy beans and seed discoloration of navy beans caused by CBB were also significantly reduced by resistance. Seed weights were reduced by 2.1–4.7% in the susceptible cultivars, but there was only a slight or no decrease or no effect on the seed weight of the CBB-resistant lines and cultivars. Generally the magnitude of the reductions in yield was much greater than the impact on seed weight, which suggests that yield losses were caused by a combination of reduced seed weight and the number of seeds per plant.


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