scholarly journals Two rice receptor-like kinases maintain male fertility under changing temperatures

2017 ◽  
Vol 114 (46) ◽  
pp. 12327-12332 ◽  
Author(s):  
Junping Yu ◽  
Jiaojiao Han ◽  
Yu-Jin Kim ◽  
Ming Song ◽  
Zhen Yang ◽  
...  
Keyword(s):  
Male Sterility ◽  
High Temperatures ◽  
Low Temperatures ◽  
Male Fertility ◽  
Molecular Mechanisms ◽  
Environmental Changes ◽  
Pollen Viability ◽  
Molecular Networks ◽  
Hybrid Seed Production ◽  
Environment Temperature

Plants employ dynamic molecular networks to control development in response to environmental changes, yet the underlying mechanisms are largely unknown. Here we report the identification of two rice leucine-rich repeat receptor-like kinases, Thermo-Sensitive Genic Male Sterile 10 (TMS10) and its close homolog TMS10-Like (TMS10L), which redundantly function in the maintenance of the tapetal cell layer and microspore/pollen viability under normal temperature conditions with TMS10 playing an essential role in higher temperatures (namely, 28 °C). tms10 displays male sterility under high temperatures but male fertility under low temperatures, and the tms10 tms10l double mutant shows complete male sterility under both high and low temperatures. Biochemical and genetic assays indicate that the kinase activity conferred by the intracellular domain of TMS10 is essential for tapetal degeneration and male fertility under high temperatures. Furthermore, indica or japonica rice varieties that contain mutations in TMS10, created by genetic crosses or genome editing, also exhibit thermo-sensitive genic male sterility. These findings demonstrate that TMS10 and TMS10L act as a key switch in postmeiotic tapetal development and pollen development by buffering environmental temperature changes, providing insights into the molecular mechanisms by which plants develop phenotypic plasticity via genotype–environment temperature interaction. TMS10 may be used as a genetic resource for the development of hybrid seed production systems in crops.

scholarly journals Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of ZmMs20 Gene Regulating Male Fertility in Maize

2019 ◽  
Vol 20 (6) ◽  
pp. 1411 ◽  
Author(s):  
Yanbo Wang ◽  
Dongcheng Liu ◽  
Youhui Tian ◽  
Suowei Wu ◽  
Xueli An ◽  
...  
Keyword(s):  
Male Sterility ◽  
Pollen Development ◽  
Male Fertility ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Hybrid Breeding ◽  
Functional Markers ◽  
Hybrid Seed Production ◽  
Functional Relationships ◽  
Zea May

Genic male sterility (GMS) mutant is a useful germplasm resource for both theory research and production practice. The identification and characterization of GMS genes, and assessment of male-sterility stability of GMS mutant under different genetic backgrounds in Zea may (maize) have (1) deepened our understanding of the molecular mechanisms controlling anther and pollen development, and (2) enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for hybrid breeding. Here, we reported a complete GMS mutant (ms20), which displays abnormal anther cuticle and pollen development. Its fertility restorer gene ZmMs20 was found to be a new allele of IPE1 encoding a glucose methanol choline (GMC) oxidoreductase involved in lipid metabolism in anther. Phylogenetic and microsynteny analyses showed that ZmMs20 was conserved among gramineous species, which provide clues for creating GMS materials in other crops. Additionally, among the 17 maize cloned GMS genes, ZmMs20 was found to be similar to the expression patterns of Ms7, Ms26, Ms6021, APV1, and IG1 genes, which will give some clues for deciphering their functional relationships in regulating male fertility. Finally, two functional markers of ZmMs20/ms20 were developed and tested for creating maize ms20 male-sterility lines in 353 genetic backgrounds, and then an artificial maintainer line of ms20 GMS mutation was created by using ZmMs20 gene, ms20 mutant, and BMS system. This work will promote our understanding of functional mechanisms of male fertility and facilitate molecular breeding of ms20 male-sterility lines for hybrid seed production in maize.

scholarly journals Poaceae-specific MS1 encodes a phospholipid-binding protein for male fertility in bread wheat

2017 ◽  
Vol 114 (47) ◽  
pp. 12614-12619 ◽  
Author(s):  
Zheng Wang ◽  
Jian Li ◽  
Shaoxia Chen ◽  
Yanfang Heng ◽  
Zhuo Chen ◽  
...  
Keyword(s):  
Male Sterility ◽  
Bread Wheat ◽  
Male Fertility ◽  
Binding Activity ◽  
Hybrid Seed Production ◽  
Hybrid Wheat ◽  
Phospholipid Binding ◽  
Allohexaploid Wheat ◽  
Molecular Nature

Male sterility is an essential trait in hybrid seed production for monoclinous crops, including rice and wheat. However, compared with the high percentage of hybrid rice planted in the world, little commercial hybrid wheat is planted globally as a result of the lack of a suitable system for male sterility. Therefore, understanding the molecular nature of male fertility in wheat is critical for commercially viable hybrid wheat. Here, we report the cloning and characterization of Male Sterility 1 (Ms1) in bread wheat by using a combination of advanced genomic approaches. MS1 is a newly evolved gene in the Poaceae that is specifically expressed in microsporocytes, and is essential for microgametogenesis. Orthologs of Ms1 are expressed in diploid and allotetraploid ancestral species. Orthologs of Ms1 are epigenetically silenced in the A and D subgenomes of allohexaploid wheat; only Ms1 from the B subgenome is expressed. The encoded protein, Ms1, is localized to plastid and mitochondrial membranes, where it exhibits phospholipid-binding activity. These findings provide a foundation for the development of commercially viable hybrid wheat.

scholarly journals Kavunda ABA Uygulamalarının Bitki Büyümesi, Çiçek Cinsiyeti ve Çiçek Tozu Kalitesine Etkileri

2018 ◽  
Vol 6 (9) ◽  
pp. 1224
Author(s):  
İlknur Solmaz ◽  
Esma Kartal ◽  
Nebahat Sarı
Keyword(s):  
Plant Growth ◽  
Male Sterility ◽  
Pollen Viability ◽  
Male Flower ◽  
Female Parent ◽  
Parent Material ◽  
Control Treatment ◽  
Flower Formation ◽  
Hybrid Seed Production ◽  
Male Flowers

It is important to use male sterility or chemical hybridization agents (CHA) to cause male sterility in the andromonoic female parent material to avoid time, labor and yield loss in hybrid seed production in melon. In this study, the effects of ABA which is a phytohormone and used as CHA in some plant species, on plant growth, male flower formation and pollen quality in Galia melon variety were investigated. In this research, two doses of 250 and 500 ppm of ABA were applied at seedling (F), at the first flowering period of hanged plants in greenhouse (İÇ) and at seedling + first flowering periods (F + İÇ). The control treatment was only sprayed with water. According to research findings, F + İÇ and F applications of 500 ppm dose of ABA were slightly suppressed the plant growth at the beginning, however this effect was lifted with the increase of temperature. While ABA applications did not prevent opening of male flowers, the number of male flowers were decreased by 62% compared to control at 500 ppm F + İÇ application. There is no quality enhancing effect of ABA applications on pollen viability and germination which both were found above 95%.

scholarly journals Male Fertility Genes in Bread Wheat (Triticum aestivum L.) and Their Utilization for Hybrid Seed Production

2021 ◽  
Vol 22 (15) ◽  
pp. 8157
Author(s):  
Manjit Singh ◽  
Marc C. Albertsen ◽  
A. Mark Cigan
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Male Fertility ◽  
Production Systems ◽  
Triticum Aestivum L ◽  
Hybrid Seed ◽  
Addition Line ◽  
Hybrid Seed Production ◽  
Wheat Varieties ◽  
The Cost

Hybrid varieties can provide the boost needed to increase stagnant wheat yields through heterosis. The lack of an efficient hybridization system, which can lower the cost of goods of hybrid seed production, has been a major impediment to commercialization of hybrid wheat varieties. In this review, we discuss the progress made in characterization of nuclear genetic male sterility (NGMS) in wheat and its advantages over two widely referenced hybridization systems, i.e., chemical hybridizing agents (CHAs) and cytoplasmic male sterility (CMS). We have characterized four wheat genes, i.e., Ms1, Ms5, TaMs26 and TaMs45, that sporophytically contribute to male fertility and yield recessive male sterility when mutated. While Ms1 and Ms5 are Triticeae specific genes, analysis of TaMs26 and TaMs45 demonstrated conservation of function across plant species. The main features of each of these genes is discussed with respect to the functional contribution of three sub-genomes and requirements for complementation of their respective mutants. Three seed production systems based on three genes, MS1, TaMS26 and TaMS45, were developed and a proof of concept was demonstrated for each system. The Tams26 and ms1 mutants were maintained through a TDNA cassette in a Seed Production Technology-like system, whereas Tams45 male sterility was maintained through creation of a telosome addition line. These genes represent different options for hybridization systems utilizing NGMS in wheat, which can potentially be utilized for commercial-scale hybrid seed production.

scholarly journals iTRAQ-Based Proteomic Analysis of Ogura-CMS Cabbage and Its Maintainer Line

2018 ◽  
Vol 19 (10) ◽  
pp. 3180 ◽  
Author(s):  
Fengqing Han ◽  
Xiaoli Zhang ◽  
Limei Yang ◽  
Mu Zhuang ◽  
Yangyong Zhang ◽  
...  
Keyword(s):  
Oxidative Phosphorylation ◽  
Male Sterility ◽  
Molecular Mechanisms ◽  
Transcriptome Profiling ◽  
Pcr Analysis ◽  
Hybrid Seed Production ◽  
Protein Species ◽  
Absolute Quantitation ◽  
Maintainer Line ◽  
Cms Line

Ogura cytoplasmic male sterility (CMS) contributes considerably to hybrid seed production in Brassica crops. To detect the key protein species and pathways involved in Ogura-CMS, we analysed the proteome of the cabbage Ogura-CMS line CMS01-20 and its corresponding maintainer line F01-20 using the isobaric tags for the relative and absolute quantitation (iTRAQ) approach. In total, 162 differential abundance protein species (DAPs) were identified between the two lines, of which 92 were down-accumulated and 70 were up-accumulated in CMS01-20. For energy metabolism in the mitochondrion, eight DAPs involved in oxidative phosphorylation were down-accumulated in CMS01-20, whereas in the tricarboxylic acid (TCA) cycle, five DAPs were up-accumulated, which may compensate for the decreased respiration capacity and may be associated with the elevated O2 consumption rate in Ogura-CMS plants. Other key protein species and pathways involved in pollen wall assembly and programmed cell death (PCD) were also identified as being male-sterility related. Transcriptome profiling revealed 3247 differentially expressed genes between the CMS line and the fertile line. In a conjoint analysis of the proteome and transcriptome data, 30 and 9 protein species/genes showed the same and opposite accumulation patterns, respectively. Nine noteworthy genes involved in sporopollenin synthesis, callose wall degeneration, and oxidative phosphorylation were presumably associated with the processes leading to male sterility, and their expression levels were validated by qRT-PCR analysis. This study will improve our understanding of the protein species involved in pollen development and the molecular mechanisms underlying Ogura-CMS.

scholarly journals Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ruimin Zhang ◽  
Jingjing Chang ◽  
Jiayue Li ◽  
Guangpu Lan ◽  
Changqing Xuan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Male Sterility ◽  
Molecular Mechanisms ◽  
Citrullus Lanatus ◽  
Anther Development ◽  
Dose Effect ◽  
Luciferase Reporter ◽  
Bhlh Transcription Factor ◽  
Transcriptional Level ◽  
Hybrid Seed Production

AbstractAlthough male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production, its underlying molecular mechanisms in Cucurbitaceae species are still largely unclear. Here, a spontaneous male-sterile watermelon mutant, Se18, was reported to have abnormal tapetum development, which resulted in completely aborted pollen grains. Map-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1 (ClATM1) encodes a basic helix-loop-helix (bHLH) transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional (BIF) domain in Se18 plants. qRT–PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6–8a of anther development. The genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated mutagenesis. Moreover, ClATM1 was significantly downregulated in the Se18 mutant, displaying a clear dose effect at the transcriptional level. Subsequent dual-luciferase reporter, β-glucuronidase (GUS) activity, and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter binding. Collectively, ClATM1 is the first male sterility gene cloned from watermelon, and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants.

Transcriptome analysis implicates involvement of long noncoding RNAs in cytoplasmic male sterility and fertility restoration in cotton

2019 ◽  
Author(s):  
Bingbing Zhang ◽  
Xuexian Zhang ◽  
Meng Zhang ◽  
Liping Guo ◽  
Tingxiang Qi ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Molecular Mechanisms ◽  
Fertility Restoration ◽  
Noncoding Rnas ◽  
Anther Development ◽  
Long Noncoding Rnas ◽  
Pcr Analysis ◽  
Hybrid Seed Production ◽  
Restorer Of Fertility

Abstract Background Hybrid cotton has greatly contributed to global increase in cotton productivity. The cytoplasmic male sterility (CMS)/restorer-of-fertility system is an important tool to exploit heterosis because it is convenient for commercial hybrid seed production. The importance of long noncoding RNAs (lncRNAs) in plant development is recognized, few analyses of lncRNAs during anther development of three-line hybrid cotton (CMS-D2 line A, maintainer line B, restorer-of-fertility line R) have been reported. Results Here, we performed transcriptome sequencing during anther development in Upland cotton carrying cytoplasmic male sterile Gossypium harknessii (D2) cytoplasm. Totally 80,695 lncRNAs were identified, in which 43,347 and 44,739 lncRNAs were differentially expressed in A–B and A–R comparisons, respectively. These lncRNAs represent functional candidates involved in CMS and fertility restoration. Gene ontology enrichment analysis indicated that cellular hormone metabolic processes and oxidation–reduction reaction processes might be involved in CMS, and cellular component morphogenesis and small molecular biosynthetic processes might participate in fertility restoration. Analysis of the putative relationship between lncRNAs and miRNAs revealed that 63 lncRNAs were identified as putative precursors of 35 miRNAs, and qRT-PCR analysis showed a similar expression pattern to that of RNA-sequencing data. Furthermore, construction of lncRNA regulatory networks indicated that several miRNA–lncRNA–mRNA networks might be involved in CMS and fertility restoration. Conclusion Our findings provide systematic identification of lncRNAs during anther development and lays a solid foundation for future investigation of the regulatory molecular mechanisms and utilization in ­­breeding of hybrid cotton.

Inheritance of maintenance and restoration of the Diplotaxis muralis (mur) cytoplasmic male sterility system in summer rape

2003 ◽  
Vol 83 (3) ◽  
pp. 515-518 ◽  
Author(s):  
T. C. Riungu ◽  
P. B. E. McVetty
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Male Fertility ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Brassica Napus L ◽  
Restorer Genes ◽  
Ready Availability ◽  
System Maintenance

The inheritance of Diplotaxis muralis (L) DC. mur cytoplasmic male sterile (CMS) system maintenance and restoration in summer rape (Brassica napus L.) was studied by crossing eight summer rape cultivars of diverse origin to a winter habit mur CMS A-line. The F1 progenies from all eight crosses were male fertile and were selfed to produce the F2 generation. The F2 generation was grown in the field and data on segregation for male fertility and sterility were recorded and analyzed. The F2 segregation data showed that mur CMS restoration in summer rape is controlled by dominant alleles at one to three restorer genes. The number of genes for the restoration of male fertility in mur CMS varied both among and, occasionally, within cultivars. Maintainer lines for mur CMS must carry recessive alleles in homozygous condition at all three restorer genes. The ready availability of summer rape restorers suggests that the mur CMS system has good potential for hybrid cultivar development and hybrid seed production in summer rape; however, maintainers in summer rape must first be discovered or developed. Key words: Cytoplasmic male sterility, canola, Brassica napus, male sterility

High Altitude hypoxia

2020 ◽  
Vol 17 ◽  
Author(s):  
Asma Babar ◽  
Kifayatullah Mengal ◽  
Abdul Hanan Babar ◽  
Shixin Wu ◽  
Mujahid Ali Shah ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
High Altitude ◽  
Molecular Mechanisms ◽  
Strong Association ◽  
Haemoglobin Concentration ◽  
Metabolic Reprogramming ◽  
Molecular Networks ◽  
Whole Genome ◽  
Gene Expressions ◽  
Transcriptional Responses

: The world highest and largest altitude area is called the Qinghai-Tibetan plateau (QTB), which harbors unique animal and plant species. Mammals that inhabit the higher altitude regions have adapted well to the hypoxic conditions. One of the main stressors at high altitude is hypoxia. Metabolic responses to hypoxia play important roles in cell survival strategies and some diseases. However, the homeostatic alterations that equilibrate variations in the demand and supply of energy to maintain organismal function in a prolonged low O2 environment persist partly understood, making it problematic to differentiate adaptive from maladaptive responses in hypoxia. Tibetans and yaks are two perfect examples innate to the plateau for high altitude adaptation. By the scan of the whole-genome, EPAS1 and EGLN1 were identified as key genes associated with sustained haemoglobin concentration in high altitude mammals for adaptation. The yak is a much more ancient mammal which has existed on QTB longer than humans, it is, therefore, possible that natural selection represented a diverse group of genes/pathways in yaks. Physiological characteristics are extremely informative in revealing molecular networks associated with inherited adaptation, in addition to the whole-genome adaptive changes at the DNA sequence level. Gene-expression can be changed by a variety of signals originating from the environment, and hypoxia is the main factor amongst them. The hypoxia-inducible factors (HIF-1α and EPAS1/HIF-2α) are the main regulators of oxygen in homeostasis which play a role as maestro regulators of adaptation in hypoxic reaction of molecular mechanisms. (Vague) The basis of this review is to present recent information regarding the molecular mechanism involved in hypoxia that regulates candidate genes and proteins. Many transcriptional responses toward hypoxia are facilitated by HIFs that change the number of gene expressions and help in angiogenesis, erythropoiesis, metabolic reprogramming and metastasis. HIFs also activate several signals highlighting a strong association between hypoxia, the misfolded proteins’ accumulation in the endoplasmic reticulum in stress and activation of unfolded protein response (UPR). It was observed that at high-altitude, pregnancies yield a low birth weight ∼100 g per1000 m of the climb. (Vague) It may involve variation in the events of energy-demanding, like protein synthesis. Prolonged hypobaric hypoxia causes placental ER stress, which in turn, moderates protein synthesis and reduces proliferation. Further, Cardiac hypertrophy by cytosolic Ca2+ raises and Ca2+/calmodulin, calcineurin stimulation, NF-AT3 pathway might be caused by an imbalance in Sarcoplasmic reticulum ER Ca2, might be adaptive in beginning but severe later.

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