The use of hydroxyproline analyses to predict the nutritional value of the protein in different animal tissues

1972 ◽  
Vol 27 (3) ◽  
pp. 475-481 ◽  
Author(s):  
Z. Dvořák
Keyword(s):  
Amino Acids ◽  
Nutritional Value ◽  
Amino Acid Content ◽  
Close Correlation ◽  
Regression Equations ◽  
Animal Tissues ◽  
Chemical Score ◽  
Limiting Amino Acids ◽  
Net Protein ◽  
Total Amino Acids

1. The amounts of available cystine and tyrosine in the protein of different animal tissues showed a close correlation with the level of hydroxyproline, and may be estimated from hydroxyproline values by regression equations.2. Estimates of ‘chemical score’ have been calculated from the content of hydroxyproline determined in a series of samples for which net protein utilization (NPU) for rats had also been determined. Chemical scores calculated as percentages of the total ‘essential+semiessential’ amino acid content of each material correlated closely with NPU, whereas scores calculated as percentages of total amino acids did not. ‘Methionine+cystine’ were calculated to be first limiting amino acids in every sample.

Blood amino acid studies: Prediction of limiting amino acids in mixtures of dietary proteins

1968 ◽  
Vol 46 (5) ◽  
pp. 707-711 ◽  
Author(s):  
S. Venkat Rao ◽  
F. J. Noel ◽  
J. M. McLaughlan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Single Amino Acid ◽  
Protein Supplements ◽  
Chick Pea ◽  
Methionine Deficiency ◽  
Chemical Score ◽  
Plasma Methods ◽  
Limiting Amino Acids

Diets containing mixtures of cereals and protein supplements were fed ad libitum to male weanling rats 18 h daily for 5 days. The cereals consisted of rice, wheat, and corn, and the supplements included peanut, soy, cottonseed, and chick pea flours. Levels of plasma lysine, methionine, threonine, trytophan, leucine, and isoleucine were determined. Two methods designated as plasma amino acid (PAA) score and PAA ratio were tested for predicting the limiting amino acids in the diets utilizing the PAA data. Chemical score values were calculated from the amino acid content of the mixtures. Growth tests were also carried out with the diets, with or without a supplement of the amino acids indicated to be limiting by PAA score and PAA ratio methods. PAA ratios, PAA scores, and protein efficiency ratio data of rats indicated that lysine and threonine were equally limiting in rice–peanut, rice–soy, rice–cottonseed, rice – chick pea, wheat–peanut, and wheat–cottonseed diets. Plasma methods were also correct in showing that lysine was the limiting amino acid in corn–peanut, corn–soy, and corn–cottonseed mixtures. Plasma methods did not indicate a methionine deficiency when this amino acid was co-limiting with lysine and threonine. Chemical score failed to detect the threonine deficiency present in 8 of 12 diets. Limiting amino acids were not established for the corn – chick pea diet. It was interesting that the effective amino acid supplement for 8 of 12 diets required threonine in addition to lysine. It was concluded that the PAA methods are effective for predicting a deficiency of a single amino acid or of two co-limiting amino acids, but less satisfactory for predicting multiple amino acid deficiencies.

Protein utilization in rats receiving a low-protein diet with various limiting amino acids

1978 ◽  
Vol 39 (1) ◽  
pp. 13-18 ◽  
Author(s):  
H. Rafalski ◽  
E. JabŁOŃski ◽  
Teresa Switoniak
Keyword(s):  
Amino Acids ◽  
Protein Utilization ◽  
Low Protein ◽  
Chemical Score ◽  
Amino Acid Score ◽  
Maize Protein ◽  
Low Levels ◽  
Limiting Amino Acids ◽  
Protein Diets ◽  
Net Protein

1. A study was made of protein utilization in rats given a variety of low-protein diets containing (g/kg) egg albumin 18, casein 49, gluten 50, or mixtures of either maize protein and gelatine 58, or casein and gelatin 37, each with supplemental methionine. The diets were limiting in leucine, tryptophan or lysine, or in both tryptophan and the sulphur-containing amino acids.2. Values obtained for net protein utilization (npu) at these low levels of nitrogen intake were markedly higher than the amino acid score calculated for the different test proteins, except with casein for which the two values were similar. The npu values agreed more closely with chemical score values based on the content of S-amino acids or isoleucine in the dietary protein.

Screening of amino acid constituents from date palm fruits

2016 ◽  
Vol 5 (10) ◽  
pp. 4972
Author(s):  
Lata Birlangi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
United Arab Emirates ◽  
Nutritional Value ◽  
Date Palm ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Cultivated Plants ◽  
Palm Fruits ◽  
Social Part

The date palm (Phoenix dactylifera L.) is one of mankind’s oldest cultivated plants. The fruit of the date palm is an important crop of the hot arid and semi-arid regions of the world. It has always played a genuine economic and social part in the lives of the people of these areas. The present objective in examining the amino acid content of different varieties of date palm fruits from Middle-East region; is to determine whether its protein could effectively supplement the nutritional value and it is also aimed in finding which variety is rich in number of amino acids. The phytochemical screening revealed the presence of eight essential amino acids and five non-essential amino acids in the date fruits. Among all the date fruit varieties taken as samples for the study, Dabbas cultivar of United Arab Emirates found to exhibit eight types of amino acids which includes five as non-essential ones. Total of thirteen amino acids were detected in the seven date cultivars. Determination of amino acid can serve as a guide to the possible nutritional value.

scholarly journals Chemical composition and nutritional quality of short-ripened rennet cheeses produced by traditional methods

2018 ◽  
Vol 74 (10) ◽  
pp. 5971-2018 ◽  
Author(s):  
PRZEMYSŁAW KNYSZ ◽  
MICHAŁ GONDEK ◽  
RENATA PYZ-ŁUKASIK ◽  
MONIKA ZIOMEK ◽  
ŁUKASZ DROZD ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Chemical Composition ◽  
G Protein ◽  
Nutritive Value ◽  
Salt Content ◽  
Amino Acid Content ◽  
Traditional Methods ◽  
Dairy Starter Cultures ◽  
Limiting Amino Acids

The aim of the study was to determine the variability in the chemical composition and nutritive value parameters of smoked and unsmoked short-ripened rennet cheeses and unsmoked long-ripened rennet cheeses produced by traditional methods. The raw material for the production of short-ripened cheeses was pasteurized cows’ milk obtained from a dairy, whereas the long-ripened cheeses were manufactured from raw cows’ milk obtained from the producer’s farm. All three varieties of cheese examined were produced with commercial dairy starter cultures. The material for the study was collected in winter, directly at the producers’ retail outlets in southern and eastern Poland. The basic chemical composition was determined according to the Polish Standards, whereas the amino acid profiles of proteins from the cheeses were determined by ion-exchange chromatography. The result analysis revealed significant differences between the different varieties of cheese in terms of their water content, ranging from 32.2% to 42.1%, as well as protein content, which varied from 25.6% to 31.6%. Fat levels ranged between 22.2% and 24%, whereas total ash content amounted to 5.1-5.8%. The significantly highest salt content was found in unsmoked short-ripened cheeses. In all three cheese varieties, the total exogenous amino acid content was comparable, ranging from 46.17 g to 47.36 g/100 g protein, and that of endogenous amino acids varied from 52 g to 53 g/100 g protein. The biological value of proteins was determined by calculating to the chemical score (CS), as described by Mitchell and Block, and the essential amino acid index (EAAI), as described by Oser. A comparison of the results with the standard chicken egg white proteins showed that the limiting amino acids for all varieties of cheese were methionine and cysteine. On the other hand, a comparison with the FAO/WHO-suggested pattern of amino acid requirements (1991) for all age groups over 1 year of age showed that the limiting amino acids were methionine and cysteine in smoked and unsmoked short-ripened cheeses, and treonine in long-ripened cheeses. Considering the chemical indices, such as CS and EAAI, it may be concluded that the traditional rennet cheeses produced in southern and eastern Poland have a favourable amino acid composition of proteins and a high nutritive value..

EVALUATION OF PROTEIN IN FOODS VIII. INFLUENCE OF QUALITY AND QUANTITY OF DIETARY PROTEIN ON NET PROTEIN UTILIZATION

1963 ◽  
Vol 41 (2) ◽  
pp. 275-281 ◽  
Author(s):  
A. B. Morrison ◽  
Z. I. Sabry ◽  
N. T. Gridgeman ◽  
J. A. Campbell
Keyword(s):  
Linear Model ◽  
Dietary Protein ◽  
Nutritional Value ◽  
Prediction Equation ◽  
Protein Utilization ◽  
New Model ◽  
Chemical Score ◽  
Net Protein ◽  
Maximum Utilization ◽  
General Prediction

The relation between the percentage of dietary Calories derived from protein (P) and the corresponding net protein utilization (N.P.U. or U) was studied with various proteins of different nutritional value. At P > 40 the experimental results (from rats) did not fit the Miller–Payne linear model. A semilogarithmic plot, however, accommodated all the data, ranging from P = 10 to P = 80. The new model yields, by extrapolation, values of maximum utilization that agree well with the protein (chemical) scores. A general prediction equation is given that enables N.P.U. to be calculated from a knowledge of P and of the chemical score of the protein or protein mixture at issue.

scholarly journals Пищевая ценность телятины как важнейший критерий оценки мясного сырья для детского питания

2018 ◽  
Vol 8 (1) ◽  
pp. 637-643
Author(s):  
T.L. Holubenko
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Value ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Baby Food ◽  
Black And White ◽  
Balanced Diet

<p><span lang="EN-US">The aim of the research is to assess the amino acid composition of veal from different genotype bull-calves concerning the usage in baby food production. A comparative analysis of the essential amino acids content in meat of calves of <span>black-and-white motley breed,</span> Aberdeen Angus breed and black and white crossbreeds grown according to the traditional technology of dairy cattle breeding hasn’t showed any significant differences. However, some differences were observed for each separate amino acid. Although the difference in amino acids was 2. 9% in favor of <span>black-and-white motley breed</span>. According to the amino acid composition, the calves meat of Charolais breed is biologically more complete than the Aberdeen Angus calves meat of the first generation in the valine content by 7.4%, isoleucine by 45.3% (P &lt;0.001), leucine by 15.2% (P &lt;0.001), lysine by 7.8%, threonine and phenylalanine + tyrosine by 6.5% (P &lt;0.05) and 7.5% (P &lt;0.01), respectively. Amino acid content was limited by the sum of amino acids phenylalanine + tyrosine (80.2%) in purebreds and methionine + cystine (83.4%) in <span>crossbreeds</span>. The other amino acids content was more than 100%; it indicates a high biological and nutritional value of veal. In the first experiment, the degree of compliance with the norms of a balanced diet is 37.4-38.2%. The human body's need for such essential amino acids as valine, isoleucine, phenylalanine + tyrosine is satisfied for more than 20%; the human body's need for leucine, lysine, and threoni9ne is satisfied for more than 30%. Veal of the Charolaise young is distinguished by higher indicators. The degree of its compliance with the norms of a balanced diet is 43.8% against 37.6% in hybrid calves. It is proved that veal obtained from young animals of different breeds in ecologically clean zones has a high biological and nutritional value, it corresponds to the indices for meat raw materials for baby food in accordance with Sanitary Norms 11-63 RB98.</span></p>

scholarly journals RESULTS OF SCREENING ASSESSMENT OF NUTRITIONAL VALUE AND AMINO ACIDS CONTENT IN THE INDUSTRIAL WORKERS DIET

2019 ◽  
pp. 40-43
Author(s):  
T.V. Mazhaeva ◽  
S.E. Dubenko
Keyword(s):  
Amino Acids ◽  
Nutritional Value ◽  
Protein Quality ◽  
Raw Materials ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Toxic Substances ◽  
Industrial Workers ◽  
Regulatory Requirements ◽  
Laboratory Methods

To decrease the toxic effects of xenobiotics on healthy workers in healthful and dietary meals, it’s important to consider not only the proper diet intake of macro- and micronutrients, but protein quality in terms of essential amino acids. A study was conducted to evaluate nutritional value and amino acids content in the industrial workers diets of two metallurgical enterprises by calculation and laboratory methods. We found that set of healthful and dietary meals at the calculation stage do not comply with regulatory requirements for fats and carbohydrates by 34 and 16 percent, respectively. According to laboratory tests, from 57 to 68 percent of sets of healthful and dietary meals do not comply with regulatory requirements for proteins, fats and carbohydrates. The discrepancy in the data on the amount of determined nutrients between the calculated and laboratory methods may be due to calculation errors, shortage and quality of raw materials, as well as a limitation of methods for determining fat and carbohydrates. With a set of healthful and dietary meals workers can satisfy the almost daily requirement for the corresponding energy expenditures for some amino acids, however, the required amount of these amino acids in workers in contact with heavy metals has not been established. It has also been found that in diets of healthful and dietary meals, the ratio of tryptophan / lysine / methionine + cystine is violated towards an excess of tryptophan, whose deamination products are highly toxic. It is necessary to continue further investigation of ready-made diets of healthful and dietary meals for nutritional value and amino acid content in order to identify factors affecting the discrepancy within research results between calculation and laboratory methods, as well as establish adequate norms for amino acids intake in workers exposed to toxic substances.

scholarly journals Chemical Composition, Nutritional Functions, and Antioxidant Activities of Honeys in Africa

2019 ◽  
Vol 63 (2) ◽  
pp. 179-200
Author(s):  
Haroon Elrasheid Tahir ◽  
Abdalbasit A. Mariod ◽  
Gusta Mahunu ◽  
Zou Xiaobo
Keyword(s):  
Amino Acids ◽  
Rural Communities ◽  
Nutritional Value ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Economic Sustainability ◽  
Polyphenol Compounds ◽  
Positive Effects ◽  
Honey Production ◽  
Total Amino Acids

AbstractHoney production in Africa is considered an integral part of folk medicine and provides an opportunity for poor rural communities to increase their economic sustainability. In this review, we provide and discuss the data available on the positive nutritional value and health properties of honey harvested from Africa. Besides sugar, honey contains protein and amino acids, with proline which is about 50% of the total amino acids, vitamins, enzymes, some minerals (Na, K, Mg, Ca, Zn, Cu, Mn, Fe, P, S), trace elements (Mn, Ni, Li, F, I, Cl, Sr, Co) and polyphenol compounds. The paper reviewed studies on the use of African honey with anti-inflammatory, antioxidant, antibacterial, antifungal, anti-breast cancer, antidiabetic and antimycotic properties and also its positive effects on infertility (asthenozoospermia), febrile neutropenia and diabetic foot ulcers.

scholarly journals Total Amino Acids by UHPLC–UV in Infant Formulas and Adult Nutritionals, First Action 2018.06

2019 ◽  
Vol 102 (5) ◽  
pp. 1574-1588 ◽  
Author(s):  
Greg Jaudzems ◽  
Joseph Guthrie ◽  
Sabine Lahrichi ◽  
Christophe Fuerer
Keyword(s):  
Amino Acids ◽  
Infant Formula ◽  
Amino Acid Content ◽  
Method Performance ◽  
Expert Review ◽  
Performance Requirements ◽  
Expert Review Panel ◽  
Single Laboratory ◽  
Total Amino Acids

Abstract Background: An acid hydrolysis ultrahigh-performance LC–UV method was evaluated for the determination of total amino acids in infant formula and adult/pediatric nutritional formula. Objective: It was assessed for compliance against AOAC INTERNATIONAL Standard Method Performance Requirements (SMPR®) established by the Stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN). Methods: A single-laboratory validation (SLV) study was conducted as a first step in the process to validate the method. In this SLV, 17 SPIFAN matrices representing a range of infant formula and adult nutritional products were evaluated for their amino acid content. Results: The analytical range was found to be within the needs for all products; some may require a dilution. Evaluation of trueness performed on Standard Reference Material 1849a (Infant/Adult Nutritional Formula) showed all compounds met the SMPR theoretical value, with exceptions for threonine and tyrosine. These may have a bias for the National Institute of Standards and Technology (NIST) data, depending on hydrolysis used in the determination of the NIST certificate of analysis. Conclusions: Based on the results of this SLV, this method met the SMPR and was approved as a First Action method by the AOAC Expert Review Panel on Nutrient Methods on August 28, 2018.

REGRESSION ESTIMATES OF LYSINE AND OTHER AMINO ACIDS IN MANITOBA BARLEY

1990 ◽  
Vol 70 (1) ◽  
pp. 333-336 ◽  
Author(s):  
K. M. DUPCHAK ◽  
D. HICKLING
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Good Predictor ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Prediction Equation ◽  
Regression Equations ◽  
Key Words Barley ◽  
Amino Acid Levels

Manitoba barley samples from the 1986 and 1987 crop years were analyzed for lysine and crude protein. The 1987 samples were also analyzed for other amino acids and for proximate components. Regression equations were developed to predict amino acid content. Crude protein was found to be a good predictor of amino acid levels. There was no effect of crop year on the prediction equation for lysine. Key words: Barley, lysine, prediction

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