scholarly journals Environmental Enrichment of Nonhuman Primates, Dogs and Rabbits Used in Toxicology Studies

2003 ◽  
Vol 31 (1_suppl) ◽  
pp. 132-137 ◽  
Author(s):  
Kathryn A.L. Bayne

The increasing emphasis on the provision of environmental enrichment to laboratory animals, vis-à-vis the USDA Animal Welfare Regulations, the Guide for the Care and Use of Laboratory Animals (NRC 1996), and a potential forthcoming policy from the USDA on the subject, can be difficult to accommodate in a toxicology research environment. A summary will be provided of current requirements and recommendations. Then, strategies for meeting regulatory requirements will be described for non-rodent animals used in toxicology research. These strategies will address methods of both social enrichment, such as pair or group housing, as well as non-social enrichment, such as cage furniture, food enrichments, and toys. In addition, the value of positive interactions with staff (e.g., through training paradigms or socialization programs) will also be discussed. Apparent in the discussion of these strategies will be an overarching recognition of the necessity to avoid introducing confounding variables into the research project and to avoid compromising animal health. The roles of the Institutional Animal Care and Use Committee (IACUC) and the attending veterinarian in helping scientists balance animal well-being, the scientific enterprise and the regulatory environment will be described.

2014 ◽  
Vol 5 (3) ◽  
Author(s):  
Casey Coke-Murphy ◽  
Matthew Buendia ◽  
Tommy Saborido ◽  
Gregg Stanwood

AbstractEnvironmental enrichment aims to improve the well-being of laboratory animals and provides an opportunity to improve experimental reliability and validity. Animals raised in more stimulating environments have improved learning and memory as well as more complex brain architecture. However, the effects of environmental enrichment on motor performance, anxiety and emotional development have been poorly studied. Moreover, most investigators studying the effects of enrichment provide extremely large and complex housing conditions to maximize the likelihood of finding effects. These situations are difficult to replicate across animal facilities and are not operationally practical. In this experiment, we investigated how simple, inexpensive disposable shelterstyle enrichment items alter behavior in C57Bl/6 and 129S6 mice. Breeding pairs were established in the presence of a Ketchum “Refuge”, Shepherd Shack “Dome”, or no enrichment. Offspring were assessed neurobehaviorally, either just after weaning (pre-adolescent, P22–P25), or as young adults (P60–P90). Major strain differences were observed in open field activity, elevated maze exploration, and Y-maze activity levels. The presence of the Refuge and/or Dome enrichment shelters significantly altered motor activity, coordination and some measures of anxiety. Mice housed in the presence of shelters were also less dominant than control mice in a tube test assay. Our experiments provide a detailed analysis of the effects of inexpensive and practical methods of housing enrichment on biobehavioral phenotypes in these two commonly used strains of laboratory mice, and suggest that the effects of these shelters on mouse neurobiology and behavior need to be rigorously analyzed before being adopted within vivariums.


1997 ◽  
Vol 31 (2) ◽  
pp. 133-143 ◽  
Author(s):  
H. A. Van de Weerd ◽  
P. L. P. Van Loo ◽  
L. F. M. Van Zutphen ◽  
J. M. Koolhaas ◽  
V. Baumans

Behavioural and psychological needs of laboratory animals generally cannot adequately be met in standard laboratory cages. Environmental enrichment, which provides a more structured environment can enhance the well-being of laboratory animals. They may perform more of their species-specific behaviour and may control their environment in a better way. An easily applicable form of enrichment for laboratory mice is nesting material. Six different types of nesting materials were evaluated in a preference test with male and female animals of two strains (C57BL/6J or BALB/c, n=48). No significant differences in preference were found between the strains or between the sexes. All mice showed a clear preference for cages with tissues or towels as compared to paper strips or no nesting material, and for cages with cotton string or wood-wool as compared to wood shavings or no nesting material. Paper-derived materials were preferred over wood-derived materials, although the results also suggest that the nature (paper or wood) of the nesting material is less important than its structure, which determines the nestability of the material. Nesting material may be a relatively simple method to contribute to the well-being of laboratory mice.


2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 161-161
Author(s):  
U. Viora ◽  
P. Ponzio ◽  
M. T. Mascarino ◽  
B. Picco ◽  
P. Guiso ◽  
...  

Background:Animal-Assisted Interventions (AAI) is the new way to indicate what was previously known as “Pet Therapy”, as activities can be done either with the conventional “pets” (dogs, cats and rabbits) or with horses and donkeys.Children with JIA have several problems in terms of adherence both due to the atavistic fear of the needle and due to nausea and vomiting - the most important side effects of Methotrexate – often since the 2-3 days before the assumption to immediately after it.Sure that animals can help children to forget this fear and to avoid the psycological conditions which enhance nausea, for the first time in Italy (and probably in Europe) it was designed a specific AAI program for these children.Objectives:To promote a general state of psycho-physical well-being in children and families about:manage of therapy; reduce discomfort and anxiety caused by entering hospital; improve self-esteem and the response to the stress generated by the execution of therapy and disease management; strengthen communication and socialization; stimulate the affective area through the activities of animal care.Methods:Dogs and cats are part of the recreational activities once a week in an equipped area in the OIRM Hospital (no alternative gateway was needed).Paediatric Rheumatologists selected two different groups of children: the first one (5 children in the pilot study) every 15 days; the second one (5 children) every month; the selection was made looking at the therapeutic scheme.Every session, one hour, has 3 clearly distinct stages:Welcome and organization: children say hello to dogs and cats, open the toolkits specifically designed for the intervention, express their state of mind and are encouraged to tell their own stories.Therapy:parents prepare and inject the drug to their children under medical or Health Professional control without discontinuation of the activities with animals.Play and socializing:children are involved in petting and other activities with animals; they are also involved in manipulative activities (design, puppets shows, modelling clay, animal care, ball retriving, etc). This step has the aim to relieve stress and discomfort due to medical procedures.Visual Analogic Scales (VAS) were part of the toolkit, to let the researchers evaluate the effects of the activity directly from the children experience.For the first time, we will control also the animal health status and wellness condition monitoring behavioural parameters and salivary cortisol level during each session.Results:The pilot project started in October 2019 and nowaday we closed 12 meetings, 4 on October, 4 on November, 3 on December and 2 on January, with the participation of 2 dogs (Golden and Labrador Retriever) and 1 cat (Devon Rex) in each one.All children love to play with animals, seek their closeness at the time of therapy and enjoy playing all together with the dogs; no one cry or refuse therapy and, since the third session, no one has nausea before, during or after the injection. Parents have reached a certain level of confidence: they stay quietly in the waiting room or go away to have a drink or to run an errand (it becomes a moment of relaxing for them too).Animals remain in healthy and wellness conditions during the activity.Conclusion:These preliminary data seem that AAI to be useful in helping patients in JIA to overcome some problems related to their pathology.Disclosure of Interests:UGO VIORA: None declared, Patrizia Ponzio: None declared, Maria Teresa Mascarino: None declared, Barbara Picco: None declared, Paolo Guiso: None declared, Eleonora Battista: None declared, Silvana Martino: None declared, Davide Montin Speakers bureau: Not relevant for the topic, Marta Dellepiane: None declared, Germana Rosso: None declared


2021 ◽  
pp. 026119292110168
Author(s):  
Mohamed Hosney ◽  
Abeer M. Badr ◽  
Sohair R. Fahmy ◽  
Ahmed Afifi ◽  
Vera Baumans ◽  
...  

Cairo University was the first academic institution in Egypt to establish an Institutional Animal Care and Use Committee (IACUC), as mandated by the World Organisation for Animal Health (OIE). Animal-based research should be performed in accordance with international regulations to monitor the humane care and use of the laboratory animals. Until 2018, the formal training of researchers in the appropriate and correct methods of animal handling during sampling and administration, as well as their husbandry demands, was an uncommon practice in Egypt. In 2018, the Egyptian Association for Animal Research Advancement (EAARA) organised the first international course in laboratory animal science (LAS), in collaboration with Utrecht University (The Netherlands) and the Faculty of Science, Cairo University, to raise researchers’ awareness and increase their knowledge of the principles that govern the humane use and care of laboratory animals. A total of 26 researchers from a number of fields (veterinary medicine, dentistry, science, medicine, pharmacy and agriculture) enrolled in the course. In the responses to the post-course questionnaire, 24 (92.3%) participants stated that the principles of animal welfare (Three Rs) were well explained. In addition, 18 (69%) participants found that the course improved their skills in animal sampling and handling. Of the 26 participants, 22 (84.6%) became aware of their responsibility towards their experimental animals and agreed that the different methods of euthanasia were well explained. In conclusion, the general assessment of the course revealed a positive outcome regarding the culture of animal care; the course was repeated a year later, and several participants were enlisted as trainers in this second course.


2017 ◽  
Vol 60 (3) ◽  
pp. 222
Author(s):  
N. KOSTOMITSOPOULOS (Ν. ΚΩΣΤΟΜΗΤΣΟΠΟΥΛΟΣ)

During the last decades there has been an increased scientific interest in the improvement of housing conditions for laboratory animals by providing them with opportunities to perform more species - specific behavioural repertoires through enriching their environment. Environmental enrichment is, by definition, any modification in the environment of the captive animals that seeks to enhance their physical and physiological wellbeing by providing stimuli that meet the animals' species-specific needs. An enrichment scheme can be focused on the social and the physical environment. The social environment of animals can be enriched by housing them together with conspecifics in pairs or in groups. Procedures to achieve group formation need careful introduction of individuals which are compatible, a factor which is strongly dependent upon age, sex and hierarchical rank. Social housing will be beneficial only if the pairs or groups are harmonious and stable. The close contact with humans could be also considered as social improvement. Strategies to improve physical environment of laboratory animals should include provision of stimuli (materials or devices) that are biologically meaningful to them, with which they can choose to interact or not and which are not harmful to them. In practice, any enrichment scheme should be well designed before its implementation. In depth knowledge of the behavioural needs of the animals is prerequisite. Close collaboration between the scientific and the technical personnel is also necessary. Environmental changes need to be carefully evaluated in order to establish whether the improvement of animal welfare has been really achieved and to determine the impact on the obtained experimental results. The assessment of improved well-being as a result of environmental changes is based on a complex of behavioural and physiological parameters.


2017 ◽  
Vol 59 (2) ◽  
pp. 168
Author(s):  
E. PARONIS (Ε. ΠΑΡΩΝΗΣ) ◽  
P. ALEXAKOS (Π. ΑΛΕΞΑΚΟΣ) ◽  
C. DIMITRIOU (Κ. ΔΗΜΗΤΡΙΟΥ) ◽  
E. BALAFAS (Ε. ΜΠΑΛΑΦΑΣ) ◽  
N. KOSTOMITSOPOULOS (Ν. ΚΩΣΤΟΜΗΤΣΟΠΟΥΛΟΣ)

One of the possible ways to improve the housing conditions of laboratory animals is to give animals opportunities to perform more species-specific behavioural repertoires through providing enrichment of their environment. Environmental enrichment is, by definition, any modification in the environment of the captive animals that seeks to enhance their physical and physiological well being by providing stimuli meeting the animals' species-specific needs. Food intake is of high concern as this should be promoting the expression of physical feeding behaviour and improves the welfare of the captive animals. The aim of the present study was to evaluate the preference of mice to receive their food from a feeding cup located inside the cage or from the classical food hopper located on the stainless steel wire lid of the cage. Twenty four male C57BL/6 mice, at the age of 5-7 weeks, originated from the breeding colony of the animal facility of the Foundation, were randomly divided into two groups. In group A (n=12) the food was supplied through the food hopper. In group Β (n=12) food was supplied through the feeding cup located in the front side of the cage, as well as through the classical food hopper on the wire lid of the cage. A statistically significant preference of the mice to use the feeding cup instead of the food hopper was noticed (p<0.05). A preference of animals to empty the feeding cup from the food pellets, eat them directly from the bedding and use the feeding cup as a shelter was also observed in all the cages of group B. Based on the above preliminary observations it is concluded that the placement of a feeding cup within the cage could improve the welfare of the animals housed in individual ventilated cages and trigger the expression of a more species-specific feeding behaviour.


Author(s):  
Fatih Yildirim ◽  
Betül A. Yildirim ◽  
Ahmet Yildiz ◽  
Kübra A. Kapakin Terim ◽  
Seyda Cengiz ◽  
...  

Bedding material, which is a significant part of rodent housing, affects the health and well-being of laboratory animals. The aim of this study was to evaluate perlite as a bedding material for rodents and to compare it with wood shavings, expanded perlite and corncobs. The animals used in this experiment were 48 male and 48 female Sprague-Dawley rats. The bedding materials collected from experimental groups were analysed microbiologically. Blood samples from rats were subjected to biochemical analysis for catalase, glutathione, glutathione peroxidase, malondialdehyde, superoxide and dismutase, and foot pad skins of rats were subjected to histopathological examination. Body weight was determined at the end of the 30-day period. Perlite as the only bedding material had no effect on body weight, and it resulted in less microbial activity compared with the wood shavings, expanded perlite and corncobs. However, using perlite alone had negative effects on the skin, the moisture percentage of bedding and stress parameters. A wood shavingsperlite combination gave better results than perlite alone and appropriate perlite and other bedding material mixtures may result in bedding materials conducive to animal health and welfare. The frequency of changing the bedding material should be limited to once weekly.


Anthrozoology ◽  
2018 ◽  
pp. 59-80
Author(s):  
Kristine Coleman ◽  
Allison Heagerty

Animal care in biomedical facilities has undergone major changes in the past few decades, including having dedicated and highly trained caretakers to oversee the physiological and psychological well-being of the animals in their charge. An important outcome of this high quality animal care is the close relationship that can develop between the caretaker and the animal. Once discouraged and considered a potential threat to scientific objectivity, such positive interactions are now encouraged by many facilities. This chapter summarises the current use of animals in scientific research, and the types of human–animal interactions that are typically found in the research environment. It then examines effects of such interactions and relationships on both the animal and the caretaker. Lastly, suggestions for facilitating positive interactions while reducing the potential costs, as well as directions for future research are provided.


2015 ◽  
Vol 13 (1) ◽  
pp. 1-3
Author(s):  
M Ariful Islam

Animal welfare is the well-being of animals.  The standards of "good" animal welfare vary considerably between different contexts. These standards are under constant review and are debated, created and revised by animal welfare groups, legislators and academics worldwide (Hewson, 2003). It is strongly linked to animal health, which similarly depends on good animal husbandry. Animal welfare is a core mandate of the veterinary community generally and veterinarians individually. This concept is articulated by many veterinary organizations in their Code of Practice, Veterinary Oath or other statements of commitment. Such statements centre on the common theme “a veterinarian should be dedicated to the benefit of society, the conservation of animal resources and the relief of suffering of animals and to promote animal wellbeing”. As scientific knowledge in the field of animal welfare expands and the expectations of society change in regard to animal care, so the commonly accepted definitions of animal welfare have evolved. Knowledge of animal welfare, particularly those in production is becoming essential to veterinary professionals across the globe.DOI: http://dx.doi.org/10.3329/bjvm.v13i1.23702Bangl. J. Vet. Med. (2015). 13 (1): 1-3


2002 ◽  
Vol 36 (4) ◽  
pp. 411-419 ◽  
Author(s):  
P.-P. Tsai ◽  
U. Pachowsky ◽  
H. D. Stelzer ◽  
H. Hackbarth

Currently, environmental enrichment is a very common means of improving animal well-being, especially for laboratory animals. Although environmental enrichment seems to be a possible way for improving the well-being of animals, the consideration of housing laboratory animals should not only focus solely on animal well-being, manpower and economics but also on the precision and accuracy of the experimental results. The purpose of the present study was to evaluate the effects of enriched cages (nest box, nesting material, climbing bar) on body weight, haematological data and final organ weights. BALB/c, C57BL/6 and A/J mice, originated from Harlan Winkelmann, were used for the experiments - 16 animals of each strain. Animals at 3 weeks of age were marked and separated randomly to enriched or non-enriched cages, in groups of four, half for each housing condition. Both cages were type III Makrolon cages, only the enriched cages contained a nest box, a wood bar for climbing and nesting material. Animals were kept in a clean animal room under specific pathogen free (SPF) conditions. Body weights were recorded every week. Blood samples were collected at 14 weeks of age (white blood cells (WBC), red blood cells (RBC), haemoglobin (HGB), and haematocrit (HCT) were analysed). At 15 weeks of age, the animals were euthanized by CO2 in their home cages, and final body weight and organ weights (heart, liver, kidney, adrenal, spleen and uterus) were recorded immediately. Although nearly all the test variables were not affected by environmental enrichment in their mean values, the enriched group showed higher coefficients of variation in many variables, and strain differences of both housing conditions were not consistent. The influences of enrichment were shown to be strain- and test-dependent. Such effects may lead to an increase in the number of animals which is necessary or may change the experimental results, especially when a study, using enriched housing conditions, focuses on strain differences. Since the same enrichment design can result in different influences, a positive or a negative or no adverse effect, due to the strain and the variables studied, researchers need to collect more information before enrichment designs are introduced into experimental plans.


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