scholarly journals Penjantanan ikan gapi, Poecilia reticulata Peters, 1859 dengan pemberian ekstrak jeroan teripang pasir (Holothuria scabra)

2019 ◽  
Vol 18 (2) ◽  
pp. 127
Author(s):  
Anugerah Saputra ◽  
Astri Wulandari ◽  
. Ernawati ◽  
Muhammad Amri Yusuf ◽  
Irvan Eriswandy ◽  
...  

Guppy fish Poecilia reticulata is one of the most popular freshwater ornamental fish species because it is easy to main-tain, and has beautiful variations of color, especially males. One method to produce male guppy is masculinization me-thod. This study aimed to determine the dose of hormone treatment from visceral organ of sea cucumber to the process masculinization of fish guppy. This study used a completely randomized design with the immersion treatment of parent guppy which has been bred into the extract solution of sea cucumber innards for 24 hours with different doses i.e. 0 mg L-1, 10 mg L-1, 20 mg L-1, 30 mg L-1 and 22 mg L-1 (metiltestosterone as positive control). The parameter measured was the percentage of male fish produced. The results showed that although the effect of the treatment was not significantly different, but it can be said to be significantly different to the treatment of 0 mg L-1 (control) to obtain the best result for masculinization of guppy at 30 mg L-1 immersion and the best survival rate on treatment of 0 mg L-1 (control). The re-sults of this study suggest that the masculinization technology on the parent stock can be done using extracts of sea cu-cumber innards which has been considered as waste.AbstrakIkan gapi Poecilia reticulata merupakan salah satu jenis ikan hias air tawar yang banyak digemari karena mudah dipe-lihara, dan memiliki variasi wama yang indah, terutama jantannya. Salah satu metode untuk menghasilkan ikan gapi jantan adalah dengan metode pembalikan kelamin melalui produksi penjantanan ikan gapi. Penelitian ini bertujuan un-tuk menentukan dosis hormon perlakuan ekstrak jeroan teripang pasir terhadap proses penjantanan ikan gapi. Penelitian  ini menggunakan rancangan acak lengkap dengan perlakuan perendaman induk ikan gapi yang telah dipijahkan ke dalam larutan ekstrak jeroan teripang pasir selama 24 jam dengan dosis berbeda, yaitu 0 mg L-1, 10 mg L-1, 20 mg L-1, 30 mg L-1 dan 22 mg L-1 (metiltestosteron sebagai kontrol positif). Parameter yang diukur adalah persentase ikan jantan yang dihasilkan. Hasil penelitian menunjukkan bahwa walaupun pengaruh antarperlakuan yang diberikan tidak berbeda nyata, tetapi dapat dikatakan berbeda nyata terhadap perlakuan 0 mg L-1 (kontrol) sehingga diperoleh hasil terbaik untuk penjantanan ikan gapi pada dosis perendaman 30 mg L-1  dan sintasan terbaik pada perlakuan 0 mg L-1 (kontrol). Hasil penelitian ini memberikan informasi bahwa teknologi penjantanan pada induk ikan gapi dapat dilakukan dengan me-manfaatkan ekstrak jeroan teripang pasir yang selama ini dianggap sebagai limbah.

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 135
Author(s):  
Muhammad Safir ◽  
Desiana Trisnawati Tobigo ◽  
Seftina F Mangitung ◽  
Madinawati Madinawati ◽  
Zainab Zainab

Banggai Cardinalfish (Pterapogon kauderni) is one of the endemic fish from the waters of the Banggai Islands, Central Sulawesi Province. One factor challenging the production of P. kauderni larvae is the male fish will nurture the eggs and larvae for 30 days, hindering next spawning cycle. One effort to overcome this problem an increase of male fish number should be performed, for example through sex reversal. This study aimed to determine the effectiveness of the hormone 17α-methyltestosterone (17α-MT) through Artemia sp. to increase male sex percentage P. kauderni. This study used a completely randomized design by testing four treatments namely feeding Artemia sp. which has been soaked with 2.0 mg of the 17α-MT hormone for two- hours given to P. kauderni larvae for 10 days (A); 20 days (B); 30 days (C), and without hormone treatment 17α-MT (control). Each treatment included control was performed in triplicates. The percentage of male sex was 93.33 % at 30 days treatment and was higher (P<0.05) compared to controls (53.3 %). Additionally, the results showed that daily growth rate, biomass gain and survival rate of all treatments were not significantly different (P>0.05) with those of control. In conclusion, Artemia sp. contained 17α-methyltestosterone enhanced male percentage of P. kaurderni. Keywords: growth, male percentage, Artemia sp., survival rate, Pterapogon kauderni


2016 ◽  
Vol 3 (2) ◽  
pp. 75
Author(s):  
Nurlina Nurlina ◽  
Zulfikar Zulfikar

Penelitian ini bertujuan untuk mendapatkan waktu perendaman yang terbaik bagi induk ikan guppy (Poecilia reticulata. Penelitian ini dilaksanakan pada bulan November sampai dengan Januari 2015 yang bertempat di Laboratorium Hatchery dan Teknologi Budidaya Fakultas Pertanian, Universitas Malikussaleh. Menggunakan metode eksperimental dan Rancangan penelitian yang digunakan Rancangan Acak Lengkap (RAL) non faktorial dengan 4 perlakuan dan 3 ulangan. Hasil penelitian menunjukkan bahwa Pengaruh lama perendaman yang Berbeda menunjukkan hasil yang berbeda nyata terhadap keberhasilan jenis kelamin jantan ikan guppy. Dengan hasil perlakuan yang terbaik terdapat pada perlakuan C dengan perendaman 15 jam dengan jumlah rata-rata 89.93%. Sedangkan yang terendah terdapat pada perlakuan (A) perendaman 9 jam yaitu (72.32%). Saran Perlu adanya penelitian lanjutan mengenai jantanisasi dengan menggunakan madu dilakukan dengan dosis yang lebih tinggi dan waktu pemaparan singkat, serta perlu di lakukan penelitian mengenai kemampuan ikan guppy untuk menyerap madu yang di berikan.This study aimed to get the best immersion time for the guppy brood fish (Poecilia reticulate). The research was conducted in November 2014 until January 2015 which was held at the Laboratory of Aquaculture Hatchery and Technology, Faculty of Agriculture, University of Malikussaleh. The method of this study wos experimental menthod and analysis used completely randomized design (CRD) with 4 treatments and 3 replications. The results showed that the soaking time influenced significantly to the success of the male sex of guppy fish. The best treatment of soaking time was a 15- hours immersion with the male ratio 89.93%. While the lowest one was obtaimed in treatment of 9 hours immersion which was of (72.32%) of male. Range Value of water quality parameters during the study, namely the temperature was 26.4 - 28. 0 ° C, pH between 6.5 -8.0. There fore Suggestions for further research by using is to implement the higher doses and shorter exposure time, as well as further rescarch is also needed to do on to ability of guppy fish in absor bigy honey.


2019 ◽  
Vol 7 (1) ◽  
pp. 13-24
Author(s):  
Tomi Malik ◽  
Mochamad Syaifudin ◽  
Mohamad Amin

Masculinization is one method of sex reversal technique that can direct the sex of fish. The aim of this research is to know the effect of different concentration ofcoconut waterto percentage of male guppy fish through immersion method.This research was conducted in August - October 2018 at Fisheries Basic Laboratory, Program Study of Aquaculture, Sriwijaya University. This research used completely randomized design (CRD) with four treatments and three replications i.e without coconut water (P0), coconut water 20%(P1), coconut water 30% (P2) and coconut water 40% (P3). The observed parameters were the percentage of male guppy, survival rate and water quality (temperature, pH, ammonia and dissolved oxigen). The result showed the percentage of male guppy was 46.66 % (P0), 70.00 % (P1), 80.00 % (P2) and 83.33 % (P3). Water  quality during maintenance were temperature 20.1 – 28.9 0C, pH 6.5 – 7.2, DO 3 – 6.8 mg/L, and ammonia 0.01 – 0.09 mg/L.  Masculinization is one method of sex reversal technique that can direct the sex of fish. The aim of this research is to know the effect of different concentration ofcoconut waterto percentage of male guppy fish through immersion method.This research was conducted in August - October 2018 at Fisheries Basic Laboratory, Program Study of Aquaculture, Sriwijaya University. This research used completely randomized design (CRD) with four treatments and three replications i.e without coconut water (P0), coconut water 20%(P1), coconut water 30% (P2) and coconut water 40% (P3). The observed parameters were the percentage of male guppy, survival rate and water quality (temperature, pH, ammonia and dissolved oxigen). The result showed the percentage of male guppy was 46.66 % (P0), 70.00 % (P1), 80.00 % (P2) and 83.33 % (P3). Water  quality during maintenance were temperature 20.1 – 28.9 0C, pH 6.5 – 7.2, DO 3 – 6.8 mg/L, and ammonia 0.01 – 0.09 mg/L. 


2018 ◽  
Vol 7 (1) ◽  
pp. 775
Author(s):  
Dimas Rizki Pratama ◽  
Henni Wijayanti ◽  
Herman Yulianto

Guppy is one of the most popular ornamental fish. In some ornamental fish, colour of aquarium can enhance the fish colour intensity. The research aims to determine the effect of different aquarium colour on Guppy colour intensity which maintenance on semi outdoor. The research was used completely Randomized Design (CRD) with 4 treatments and 3 replications (maintenance guppy in colourless aquarium, black coloured aquarium, yellow coloured aquarium, silver coloured aquarium). The Guppy fish used is 60 days old that kept on aquarium 30 cm x 20 cm x 30 cm and the water volume is 4 liters/aquarium. Research was conducted for 40 days, fish amount is 8 fish/aquarium. Colour intensity data were analyzed by using ANOVA and the significantly different results followed by a further test of Least Significant Difference (LSD). Observed parameters were the colour intensity, pH, and temperature. The results showed that maintenance Guppy fish under different colour of aquarium gave the effect on Guppy colour intensity, the best effecton colour intensity Guppy fish show at the colourless aquarium.


2018 ◽  
Vol 6 (2) ◽  
pp. 117-122
Author(s):  
Sefti Heza Dwinanti ◽  
M. Hanggara Putra ◽  
Ade Dwi Sasanti

ABSTRACT               Masculinization is a method of  sex reversal technique that can direct sexuality from female to male. The purpose of this study was to determine the effects of coconut water on masculinization in guppy by immersion administration. The study used a completely randomized design (CRD) with three treatments and three replications. After 12 days post spawning, female guppies were immersed for 8 hours.The concentrations were : 0%(P0), 20% (P1), and 40% (P2). The parameters were observed including percentage of male, survival rate and water quality (thermal, pH and kalium concentration). Baby guppies were reared until sex can be determined which were 42 days of culture. The result showed that the percentage of male guppy fish was 54.44% (P0), 77.78% (P1) and 90.00% (P2). The survival rate of  larval during maintenance was 100% for all treatments. Water quality during maintenance was 27.0-28.40C for temperature and 6.38-7.20 for pH. To sum up, this experiment proved that coconut water can be used for masculinization by immersed pregnant female broadstock and it produced 90% male when immersed with 40% of coconut water. Keywords : Coconut water, guppy (Poecilia reticulata), masculinization


2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.


PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus


2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Muhammad Masyarul Rusdani, Sadikin Amir Saptono Waspodo dan Zaenal Abidin

ABSTRAKPenelitian ini bertujuan untuk menguji kinerja bakteri probiotik Bacillus spp. dan prebiotik molase dengan dosis berbeda terhadap tingkat kelangsungan hidup dan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Penelitian ini menggunakan motede eksperimen dengan rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan, yaitu kontrol (K), Bacillus spp. 10 ml/kg pakan (A); Bacillus spp. 10 ml/Kg pakan + molase 10 v/v (B); Bacillus spp. 20 ml/Kg pakan (C); dan Bacillus spp. 20 ml/Kg pakan + molase 10 v/v(D). Hasil yang diperoleh menunjukkan variasi untuk total bakteri, yaitu dari 2,24x108 hingga 3,78x108 CFU/ml. Demikian juga dengan total Bacillus spp. dari 1,07x106 hingga 2,02x107 CFU/ml. Adapun untuk pengamatan tingkat kelangsungan hidup dan laju pertumbuhan tidak menunjukkan hasil yang signifikan. Meski demikian, perlakuan D memiliki hasil yang relatif lebih baik dengan nilai SR dan LPH berturut-turut 96,67% dan 1,01 %/hari. Kata kunci : probiotik Bacillus spp., prebiotik molase,  total bakteri dan Bacillus spp., tingkat kelangsungan hidup, laju pertumbuhan harian  ABSTRACTThis study was aimed to determine the good combination of probiotic bacillus spp. and prebiotic molasses with different doses to survival rate and growth performance of tilapia (Oreochromis niloticus). this study has used experimental design with completely randomized design (CRD) and five treatment, i.e. Control (K), without probiotic and prebiotic; Bacillus spp. 10 ml/kg feed (A); Bacillus spp. 10 ml/Kg feed + molasses 10 v/v (B); Bacillus spp. 20 ml/Kg feed (C); dan Bacillus spp. 20 ml/Kg feed + molasses 10 v/v (D). The results showed variation for total bacteria and total Bacillus spp. The average of total bacteria obtained in this study ranged from 2,24x108 to 3.78x108 CFU/ml. Likewise, the total Bacillus spp. were obtained from 1.07x106 to 2.02x107 CFU/ml. While for survival rate (SR) and the growth rate (DGR) did not showed significant results. However, treatment D has a relatively better then treatment else with SR and DGR respectively 96.67% and 1.01%.day-1. Keywords : probiotic Bacillus spp., prebiotic molasses, total bacteria and Bacillus spp., survival rate, specific growth rate.


2016 ◽  
Vol 16 (2) ◽  
pp. 76
Author(s):  
Wasir Ibrahim ◽  
Rita Mutia ◽  
Nurhayati Nurhayati ◽  
Nelwida Nelwida ◽  
Berliana Berliana

ABSTRAK. Penelitian ini bertujuan untuk mengetahui penggunaan kulit nanas fermentasi ke dalam ransum yang mengandung gulma berkhasiat obat terhadap konsumsi nutrient ayam broiler. Penelitian ini menggunakan DOC jantan strain cob umur 2 hari sebanyak 200 ekor yang dipelihara selama 42 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. P0 = 0% tepung kulit nanas fermentasi (TKNF) dalam ransum mengandung 0% gulma obat(GO), (kontrol positif), P1 = 0% TKNF dalam ransum mengandung 2 % gulma obat (GO),(kontrol negatif), P2 = 7.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P3 = 15% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P4 = 22.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat. Parameter yang diamati kandungan gizi kulit nanas sebelum dan sesudah fermentasi dan konsumsi nutrient. Pengaruh yang nyata terhadap parameter yang diamati dilanjutkan dengan Uji Jarak Berganda Duncan, Data yang diperoleh dari setiap parameter dianalisis ragam (ANOVA) menggunakan bantuan software SPSS 16.0. Dari hasil penelitian disimpulkan bahwa penggunaan kulit nanas yang fermentasi dengan yoghurt dalam ransum yang mengandung gulma berkhasiat obat pada taraf 15% memberikan hasil terbaik pada konsumsi nutrient ayam broiler. (Fermented pineapple peel supplementation with addition of medicinal weeds on nutrient intake consumption of broiler chicken) ABSTRACT. The aim of this study was to determine the effect of using fermented pineapple peel in the ration containing medicinal weeds on nutrient consumption and performance of broiler . Two hundred 2 days male broiler chicken cob strain were used in this study and kept for 42 days. Completely randomized design (CRD)was applied with 5 treatments and 5 replicates. Treatments consisted of P0 = 0% of fermented pineapple peels meal (FPPM) in ration containing 0% medicinal weed (MW), positive control). P1 = 0% of fermented pineapple peels meal (FPPM) in the ration containing 2 % medicinal weed (MW), negative control). P2 = 7.5% of FPPM in the ration containing 2 % medicinal weed (MW). P3 = 15% of FPPM in the ration containing 2 % medicinal weed (MW). P4 = 22,5% of FPPM in the ration containing 2 % medicinal weed (MW). The parameters measured was nutrien intake before and after fermented peel and consumption. The observed data were analyzed by statistical product and service solution (SPSS 16.0). Results showed that use of fermented pineapple peel meal up to 15% with addition medicinal weeds was the best on nutrient consumption of broiler chicken.


2018 ◽  
Vol 1 (1) ◽  
pp. 51
Author(s):  
Sri Yuningsih Noor ◽  
Rano Pakaya

The purpose of this study was to determine the effect of the addition of probiotic EM4 (Evective Microorganism-4) in feed to growth and gouramy ointment (Osprhronemus gouramy) at the Freshwater Fish Seed Development Center in Gorontalo Province. The method of this research is experimental method and data processing using completely Randomized Design with 4 treatments 3 replicates. The treatment is done with different doses which in treatment A (Without Probiotic), B ( probiotic 1 cc), C (probiotic 2 cc), D (probiotic 3 cc). The parameters observed were growth rate, Feed Conversion (KP). Feed Efficiency (EP), and Absolute Length (PM). The data were tested continued using the LSD test (Least Significance Different). The results showed that the effect of giving EM4 probiotic with different dose to the growth rate of gouramy fish (Osprhronemus gouramy) gave a very real effect, and from the four research results it was found that treatment D with 3 cc dose was the highest value, followed by C 2 treatment cc, treatment B with a dose of 1 cc and the lowest is in treatment A (Control). Keywords : Probiotic EM-4, gouramy (Osprhronemus gouramy), Growth.    


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