scholarly journals Accumulation of PDGF B and cell-binding forms of PDGF A in the extracellular matrix.

1993 ◽  
Vol 121 (5) ◽  
pp. 1153-1163 ◽  
Author(s):  
J L Kelly ◽  
A Sánchez ◽  
G S Brown ◽  
C N Chesterman ◽  
M J Sleigh
Keyword(s):  
Matrix Material ◽  
Cell Types ◽  
Cell Binding ◽  
Laser Confocal Microscopy ◽  
Primary Transcript ◽  
Matrix Deposition ◽  
Cell Extracts ◽  
Binding Forms ◽  
Attachment Sites ◽  
The Matrix

PDGF is a powerful mitogen initially identified within platelets, but also shown to be produced by a wide variety of cell types. PDGF is encoded on two separate genes. These give rise to three polypeptides, PDGF B and two forms of PDGF A (SA and LA), resulting from alternative splicing of the PDGF A gene primary transcript. We report that in CHO cells transfected with PDGF gene constructs and producing moderate levels of PDGF homodimers, much of the PDGF LA and B produced, but little if any SA, is found in the matrix laid down beneath the cells. Immunoreactive PDGF in cells, and in matrix below expressing cells, was visualized by laser confocal microscopy. Western blotting of protein in matrix extracts, cell extracts, and secreted into the growth medium was used to demonstrate that the range of PDGF A polypeptides seen in the matrix was overlapping with those reported previously to be cell associated in cell types such as NIH3T3 and COS 7. This suggests that attachment to matrix or cell surface may be alternative fates for these polypeptides, with fate dependent on the characteristics of the producing cells. Immunoreactive PDGF A and B could be partially released by incubation of matrix material with heparin but not with other glycosaminoglycans. Digestion of matrix with chondroitin ABC lyase but not heparitinase or collagenase displaced some PDGF from its attachment sites. The results indicate attachment of PDGF to matrix proteoglycans, at least partly through the glycosaminoglycan moieties, and perhaps to additional components. The significance of matrix deposition for PDGF action is discussed.

The influence of different geometries of matrix/scaffold on the remodeling process of a bone and bioresorbable material mixture with voids

2015 ◽  
Vol 22 (5) ◽  
pp. 969-987 ◽  
Author(s):  
Ivan Giorgio ◽  
Ugo Andreaus ◽  
Tomasz Lekszycki ◽  
Alessandro Della Corte
Keyword(s):  
Bone Growth ◽  
Matrix Material ◽  
Porous Solids ◽  
Artificial Material ◽  
Matrix Deposition ◽  
Aspect Ratios ◽  
Adopted Model ◽  
The Matrix ◽  
Bioresorbable Material

Since internal architecture greatly influences crucial factors for tissue regeneration, such as nutrient diffusion, cell adhesion and matrix deposition, scaffolds have to be carefully designed, keeping in mind case-specific mechanical, mass transport and biological requirements. However, customizing scaffold architecture to better suit conflicting requirements, such as biological and mechanical ones, remains a challenging issue. Recent advances in printing technologies, together with the synthesis of novel composite biomaterials, have enabled the fabrication of various scaffolds with defined shape and controlled in vitro behavior. Thus, the influence of different geometries of the assemblage of the matrix and scaffold on the remodeling processes of living bone and artificial material should be investigated. To this end, two implant shapes are considered in this paper, namely a circular inclusion and a rectangular groove of different aspect ratios. A model of a mixture of bone tissue and bioresorbable material with voids was used to numerically analyze the physiological balance between the processes of bone growth and resorption and artificial material resorption in a plate-like sample. The adopted model was derived from a theory for the behavior of porous solids in which the matrix material is elastic and the interstices are void of material.

scholarly journals A Force Based Model of Individual Cell Migration With Discrete Attachment Sites and Random Switching Terms

2013 ◽  
Vol 135 (7) ◽  
Author(s):  
J. C. Dallon ◽  
Matthew Scott ◽  
W. V. Smith
Keyword(s):  
Cell Migration ◽  
Individual Cell ◽  
Cell Types ◽  
Cell Binding ◽  
Stochastic Nature ◽  
Attachment Sites ◽  
Random Switching ◽  
Cell Motion ◽  
Cellular Forces ◽  
Insight Into

A force based model of cell migration is presented which gives new insight into the importance of the dynamics of cell binding to the substrate. The main features of the model are the focus on discrete attachment dynamics, the treatment of the cellular forces as springs, and an incorporation of the stochastic nature of the attachment sites. One goal of the model is to capture the effect of the random binding and unbinding of cell attachments on global cell motion. Simulations reveal one of the most important factor influencing cell speed is the duration of the attachment to the substrate. The model captures the correct velocity and force relationships for several cell types.

scholarly journals The kinesin KIF9 and reggie/flotillin proteins regulate matrix degradation by macrophage podosomes

2011 ◽  
Vol 22 (2) ◽  
pp. 202-215 ◽  
Author(s):  
Susanne Cornfine ◽  
Mirko Himmel ◽  
Petra Kopp ◽  
Karim el Azzouzi ◽  
Christiane Wiesner ◽  
...  
Keyword(s):  
Matrix Material ◽  
Cell Types ◽  
Transport Processes ◽  
Cell Periphery ◽  
Matrix Degradation ◽  
Mediated Effects ◽  
C Terminus ◽  
The Matrix ◽  
Dependent Transport ◽  
Interfering Rna

Podosomes are actin-based matrix contacts in a variety of cell types, most notably monocytic cells, and are characterized by their ability to lyse extracellular matrix material. Besides their dependence on actin regulation, podosomes are also influenced by microtubules and microtubule-dependent transport processes. Here we describe a novel role for KIF9, a previously little-characterized member of the kinesin motor family, in the regulation of podosomes in primary human macrophages. We find that small interfering RNA (siRNA)/short-hairpin RNA–induced knockdown of KIF9 significantly affects both numbers and matrix degradation of podosomes. Overexpression and microinjection experiments reveal that the unique C-terminal region of KIF9 is crucial for these effects, presumably through binding of specific interactors. Indeed, we further identify reggie-1/flotillin-2, a signaling mediator between intracellular vesicles and the cell periphery, as an interactor of the KIF9 C-terminus. Reggie-1 dynamically colocalizes with KIF9 in living cells, and, consistent with KIF9-mediated effects, siRNA-induced knockdown of reggies/flotillins significantly impairs matrix degradation by podosomes. In sum, we identify the kinesin KIF9 and reggie/flotillin proteins as novel regulators of macrophage podosomes and show that their interaction is critical for the matrix-degrading ability of these structures.

scholarly journals An instructive role for the interstitial matrix in tissue patterning: tissue segregation and intercellular invasion.

1990 ◽  
Vol 110 (4) ◽  
pp. 1439-1455 ◽  
Author(s):  
P B Armstrong ◽  
M T Armstrong
Keyword(s):  
Organ Culture ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Cell Types ◽  
Mesenchymal Cells ◽  
Myocardial Cells ◽  
Matrix Deposition ◽  
The Matrix ◽  
Cardiac Mesenchyme ◽  
Heart Wall

Intercellular invasion is the intrusion of the cells of one tissue into space occupied by a second tissue. The alternative situation to invasion, one characteristic of most coherent tissues, is segregation, with identifiable boundaries existing between contiguous tissues. The interfaces between mesenchymal and myocardial tissues in the developing avian heart show a profoundly different character in different regions of the heart: the interface between epicardial mesenchyme and heart wall myocardium is planar, without intermingling of the two cell types, whereas the interface between endocardial cushion mesenchyme and myocardium is diffuse, with extensive invasion of both tissue types across the border to produce intermingling of the two tissues. Thus, invasion and tissue segregation coexist in different regions of the mesenchyme-myocardium contact zone. Investigation of the involvement of the interstitial matrix in invasion and segregation has been conducted by maintaining the two tissues in mutual contact in organ culture. Investigation of the mechanisms by which the two cell types sort out in randomized chimeric tissue reaggregates has provided insight into the conditions for tissue segregation. We have modeled invasion in organ culture by fusing aggregates of myocardial cells with aggregates of cardiac mesenchymal cells. Cells of both tissues invaded the partner aggregate during a period of 1-3 d of coculture. Both invasion and segregation in the aggregates appear to depend on the presence or absence of a fibronectin-rich interstitial matrix elaborated by the cardiac mesenchyme. During sorting, the matrix appears selectively in regions occupied by the mesenchyme. Under conditions of culture that are nonpermissive for matrix deposition, sorting fails to occur. Stimulation of matrix deposition by addition of serum, transforming growth factor beta, or isolated matrix itself is accompanied by sorting out of the two tissues. Sorting out is blocked reversibly by inclusion of the fibronectin adhesion site peptide, GRGDSP. Invasion of fused aggregates is preceded by a redistribution of the fibronectin-containing matrix of the mesenchymal aggregate such that matrix-poor regions come to occupy the interface with the myocardial partner aggregate. The invasion that ensues involves mesenchymal cells emigrating from, and myocardial cells intruding into, matrix-poor regions of the mesenchymal aggregate.(ABSTRACT TRUNCATED AT 400 WORDS)

Polishing process effect on the image of dispersed precipitates

1984 ◽  
Vol 42 ◽  
pp. 534-535
Author(s):  
C.T. Hu ◽  
C.W. Allen
Keyword(s):  
Matrix Material ◽  
Specimen Preparation ◽  
Second Phase ◽  
Precipitate Particle ◽  
Polishing Process ◽  
Second Phase Particles ◽  
The Matrix ◽  
Surface Profiles ◽  
Thinning Process

One important problem in determination of precipitate particle size is the effect of preferential thinning during TEM specimen preparation. Figure 1a schematically represents the original polydispersed Ni3Al precipitates in the Ni rich matrix. The three possible type surface profiles of TEM specimens, which result after electrolytic thinning process are illustrated in Figure 1b. c. & d. These various surface profiles could be produced by using different polishing electrolytes and conditions (i.e. temperature and electric current). The matrix-preferential-etching process causes the matrix material to be attacked much more rapidly than the second phase particles. Figure 1b indicated the result. The nonpreferential and precipitate-preferential-etching results are shown in Figures 1c and 1d respectively.

Application of cross correlation to HREM images of surfaces

1987 ◽  
Vol 45 ◽  
pp. 754-755
Author(s):  
D. E. Luzzi ◽  
L. D. Marks ◽  
M. I. Buckett
Keyword(s):  
Cross Correlation ◽  
A Priori ◽  
Matrix Material ◽  
Correlation Method ◽  
Accurate Knowledge ◽  
Complex Image ◽  
Fourier Filtering ◽  
The Matrix ◽  
Low Pass ◽  
Data Reduction Technique

As the HREM becomes increasingly used for the study of dynamic localized phenomena, the development of techniques to recover the desired information from a real image is important. Often, the important features are not strongly scattering in comparison to the matrix material in addition to being masked by statistical and amorphous noise. The desired information will usually involve the accurate knowledge of the position and intensity of the contrast. In order to decipher the desired information from a complex image, cross-correlation (xcf) techniques can be utilized. Unlike other image processing methods which rely on data massaging (e.g. high/low pass filtering or Fourier filtering), the cross-correlation method is a rigorous data reduction technique with no a priori assumptions.We have examined basic cross-correlation procedures using images of discrete gaussian peaks and have developed an iterative procedure to greatly enhance the capabilities of these techniques when the contrast from the peaks overlap.

Impact Strength, Flexural Modulus and Wear Rate of PMMA Composites Reinforced by Eggshell Powders

2020 ◽  
Vol 38 (7A) ◽  
pp. 960-966
Author(s):  
Aseel M. Abdullah ◽  
Hussein Jaber ◽  
Hanaa A. Al-Kaisy
Keyword(s):  
Impact Strength ◽  
Wear Rate ◽  
Flexural Modulus ◽  
Matrix Material ◽  
Weight Fraction ◽  
Pure Pmma ◽  
Calcination Process ◽  
The Matrix ◽  
The Impact ◽  
Interface Bond

In the present study, the impact strength, flexural modulus, and wear rate of poly methyl methacrylate (PMMA) with eggshell powder (ESP) composites have been investigated. The PMMA used as a matrix material reinforced with ESP at two different states (including untreated eggshell powder (UTESP) and treated eggshell powder (TESP)). Both UTESP and TESP were mixed with PMMA at different weight fractions ranged from (1-5) wt.%. The results revealed that the mechanical properties of the PMMA/ESP composites were enhanced steadily with increasing eggshell contents. The samples with 5 wt.% of UTESP and TESP additions give the maximum values of impact strength, about twice the value of the pure PMMA sample. The calcination process of eggshells powders gives better properties of the PMMA samples compared with the UTESP at the same weight fraction due to improvements in the interface bond between the matrix and particles. The wear characteristics of the PMMA composites decrease by about 57% with increases the weight fraction of TESP up to 5 wt.%. The flexural modulus values are slightly enhanced by increasing of the ESP contents in the PMMA composites.

scholarly journals Self-Reinforced Nylon 6 Composite for Smart Vibration Damping

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1235
Author(s):  
Bidita Salahuddin ◽  
Rahim Mutlu ◽  
Tajwar A. Baigh ◽  
Mohammed N. Alghamdi ◽  
Shazed Aziz
Keyword(s):  
Matrix Material ◽  
Nylon 6 ◽  
Vibration Damping ◽  
Vibration Energy ◽  
Damping Factor ◽  
Passive Vibration Control ◽  
Reinforced Composite ◽  
Coiled Structure ◽  
The Matrix ◽  
3D Printed

Passive vibration control using polymer composites has been extensively investigated by the engineering community. In this paper, a new kind of vibration dampening polymer composite was developed where oriented nylon 6 fibres were used as the reinforcement, and 3D printed unoriented nylon 6 was used as the matrix material. The shape of the reinforcing fibres was modified to a coiled structure which transformed the fibres into a smart thermoresponsive actuator. This novel self-reinforced composite was of high mechanical robustness and its efficacy was demonstrated as an active dampening system for oscillatory vibration of a heated vibrating system. The blocking force generated within the reinforcing coiled actuator was responsible for dissipating vibration energy and increase the magnitude of the damping factor compared to samples made of non-reinforced nylon 6. Further study shows that the appropriate annealing of coiled actuators provides an enhanced dampening capability to the composite structure. The extent of crystallinity of the reinforcing actuators is found to directly influence the vibration dampening capacity.

Investigation on the Influence of Pressure Terms in a Volume-Averaged Energy Balance in the Modelling of Liquid Composite Moulding Processes

2019 ◽  
Vol 809 ◽  
pp. 480-486
Author(s):  
Rohit George Sebastian ◽  
Christof Obertscheider ◽  
Ewald Fauster ◽  
Ralf Schledjewski
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Energy Balance ◽  
Matrix Material ◽  
Energy Balance Equation ◽  
Manufacturing Processes ◽  
Composite Manufacturing ◽  
Liquid Composite Moulding ◽  
The Matrix ◽  
Computational Fluid Dynamics Cfd

The growing use of composite materials has generated interest in improving and optimising composite manufacturing processes such as Liquid Composite Moulding (LCM). In LCM, dry preforms are placed in a mould and impregnated with the matrix material. The efficiency of filling the moulds can be improved by using Computational Fluid Dynamics (CFD) filling simulations during the design of the mould. As part of an on-going effort to develop a CFD tool for the simulation of LCM processes, a volume averaged energy balance equation has been derived and implemented in a custom OpenFOAM solver. The energy balance is implemented in a custom OpenFOAM solver with and without the pressure terms for comparison with results from RTM experiments. It is found that the pressure terms do not significantly influence the results for LCM processes.

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