scholarly journals ISOLATION OF PURIFIED COPPER PROTEIN FROM HORSE LIVER

1954 ◽  
Vol 37 (4) ◽  
pp. 433-439 ◽  
Author(s):  
Mostafa S. Mohamed ◽  
David M. Greenberg
Keyword(s):  
Copper Content ◽  
Catalytic Properties ◽  
Copper Acetate ◽  
The Body ◽  
Pure Form ◽  
Acetate Solution ◽  
Copper Protein ◽  
Green Color ◽  
Horse Liver

1. A procedure is described for isolating a copper protein from horse liver in pure form, in a state that appears to be crystalline. 2. This copper protein is colored blue-green, and its copper content varied between 0.3 and 0.4 per cent. No indication of any catalytic properties was obtained in the limited number of tests made. Because of its variable copper content, the protein may function for the storage of copper in the body. 3. The copper could be removed partly from the protein by dialysis against cyanide, and a colorless crystalline protein was obtained by lowering the pH to 5.0, followed by dialysis. The original blue-green color of the protein could be restored by dialysis against copper acetate solution.

scholarly journals Morphological characteristics of respiratory and digestive organs of Roman snail (Helix pomatia L., 1758)

2020 ◽  
Vol 22 (97) ◽  
pp. 7-12
Author(s):  
M. P. Horvat ◽  
R. S. Dankovych
Keyword(s):  
Helix Pomatia ◽  
Morphological Characteristics ◽  
Internal Surface ◽  
The Body ◽  
Digestive Cells ◽  
Green Color ◽  
Excretory Function ◽  
Intracellular Digestion ◽  
Snail Helix Pomatia ◽  
Calcium Cells

The aim of this work was to study the structure of lung and hepatopancreas of Roman snail (Helix of pomatia of L., 1758). The study found that the lung occupies the lower turn of shell and presented by a saccate cavity, in the wall of that there are a kidney and heart with a pericardium, and also a rectum and ureter pass. An external surface of lungs covered by a shell and covered by an epidermis. An internal surface is covered by a flat ciliated epithelium and forms numerous folds in which pulmonary vessels and lacunae are accommodated. The branches of pulmonary vein have a thick muscular wall, that consists of circular and longitudinal muscular layers. An internal surface of lungs covered by the layer of mucus. Inhalation and exhalation are carried out due to reduction and relaxation of muscles of dorsal wall of the body that is named a “diaphragm”. Gas exchange occurs through the hemolymphatic capillaries of the lung wall. Respiratory motions take place not rhythmically, but through the different intervals of time depending on a requirement in oxygen. The frequency of pneumostome closing and opening is typically one time in a minute. At subzero humidity of atmospheric air of pneumostome closed by a mantle, and also one (or a few) epiphragms. The hepatopancreas (“liver” or liver gland) is in the upper rotation of the sink and formed by two parts: right and left, from which two liver ducts enter into the stomach respectively. The liver gland consists of many acinuss, surrounded by connecting tissue, that contains small number of muscular fibres. Calcium cells have a pyramidal form and usually do not reach the lumen of the acinus. Cytoplasm of calcium cells contains inclusions: grains of phosphoricacid lime and drops of fat. The digestive cells of the hepatopencreas are more elongated, often clavicular. Сytoplasm of digestive cells is loose and vacuolated and contain inclusions of yellow-green color. Enzyme cells on histopreparations are difficult to distinguish from digestive ones. They contain transparent vacuoles with a large round inclusion of yellow-green color, which consists of a cluster of several grains of different sizes. Hepatopancreas performs the following functions: secretory (enzyme cells), absorption and intracellular digestion (digestive cells), preservation of nutrients and calcium (calcium cells), and also excretory function.

Establishing the limits of the permissible content of chemical elements in the wool of representatives of the family Hamster Cricetidae using centile scales

2020 ◽  
Vol 1 (9) ◽  
pp. 72-77
Author(s):  
M. V. Stepanova ◽  
◽  
V. A. Ostapenko ◽  
Keyword(s):  
Heavy Metals ◽  
Copper Content ◽  
Chemical Elements ◽  
Federal District ◽  
The Body ◽  
Average Content ◽  
Absorption Method ◽  
Opposite Trend ◽  
The Family ◽  
Heavy Metals And Arsenic

The aim of the study was to study the content of some heavy metals and arsenic in members of the Cricetidae family of hamsters and to develop centile scales for assessing the level of accumulation of these elements for the Central Federal District. In 2018–2020, hamster`s wool was studied for the accumulation of zinc, copper, iron, lead, cadmium and arsenic by the atomic absorption method. The data on the characteristics of the content of chemical elements in different sexes of animals are given. In the course of the study, it was found that, according to the value of the average content in the wool of animals of the family, the studied elements form the following decreasing series: Fe> Zn> Pb> Cu> Cd> As. When studying the features of the accumulation of chemical elements by different sexes, a significant increase in the concentration of zinc, iron, cadmium and arsenic in the wool of males was established, compared with females, depending on the species, from 1,38 to 3,98; from 1,28 to 1,52; from 2,00 to 304,00 times, respectively. With regard to the accumulation of copper in the body, the opposite trend is observed. In females, its concentration is 1,5–3,19 times higher. In the studied sample of animals, an increase in copper content was revealed in 44,83% of individuals and a decrease in cadmium in 44,82%.

scholarly journals Peculiarities of fish phosphorus balance at a high Cu2+ content in water

2019 ◽  
Vol 77 (1) ◽  
pp. 75-79
Author(s):  
V. Gandziura ◽  
N. Korevo
Keyword(s):  
Feed Efficiency ◽  
Aquatic Environment ◽  
Sharp Increase ◽  
Copper Content ◽  
Phosphorus Content ◽  
The Body ◽  
Trophic Groups ◽  
Trophic Conditions ◽  
Phosphorus Balance ◽  
Phosphorus Excretion

The essential infringements of fish phosphorus balance at elevated concentrations of Cu2+ in water was established – a sharp increase of intensity of phosphorus excretion, which ultimately leads to a significant decrease in its content in the body of fish. The peculiarities of the phosphorus balance of fish of various trophic groups: zooplankton phage, benthophages and ichthyophages have been established. It was found out how trophic conditions effect on the components of the phosphorus balance. It was found out how trophic conditions affect the components of the phosphorus balance. Bentofages and zooplankton- phages at the conditions of higher copper content have more pronounced disorders the phosphorus balance then ichthyophage-predators at the same time, even with an increase in the intensity of phosphorus excretion due to the increased copper content in the aquatic environment, was noted by minimal changes in its content in the body, due to the chemical composition of the food of these trophic groups. It was shown that when the content of Cu2+ in water is 10 µg / l of feed use with a high phosphorus content (3.0 %), it can compensate for its loss due to increased excretion, normalize its natural content in the body and increase the growth rate of fish and their feed efficiency. It is proposed to use the intensity of phosphorus excretion for the diagnosis of toxic pollution of the aquatic environment by heavy metals. At the same time, fish of various trophic groups should be used, with the exception of predators

scholarly journals Adsorption of Lead (Ii) Ions by Ecofriendly Copper Oxide Nanoparticles

2019 ◽  
Vol 35 (6) ◽  
pp. 1731-1736 ◽  
Author(s):  
G Sreekala ◽  
Beevi A Fathima ◽  
B Beena
Keyword(s):  
Waste Water ◽  
Metal Ion ◽  
Copper Acetate ◽  
Copper Oxide Nanoparticles ◽  
Batch Adsorption ◽  
Acetate Solution ◽  
Aqueous Leaf Extract ◽  
Simarouba Glauca ◽  
20 Nm ◽  
Cuo Nanoparticle

The present investigation is on the application of green synthesized CuO nanoparticles for elimination of lead (II) from waste water. Nano CuO was prepared from aqueous copper acetate solution and aqueous leaf extract of Simarouba glauca plant. The prepared nano CuO was characterized by XRD, FT-IR, UV, SEM and TEM. The nano CuO synthesized by this method was spherical in shape with particle size nearly 20 nm. The adsorption of lead (II) ions on nano CuO under various parameters such as amount of catalyst, concentration of metal ion and pH were studied using batch adsorption experiments. Experimental results indicate that the green synthesized CuO nanoparticle is a very good adsorbent for the efficient removal of Lead (II) from waste water. Optimum conditions for 95% adsorption of Pb2+ on CuO nanoparticle are pH 6, amount of catalyst 0.05g and concentration of metal ion 10mgL-1.

scholarly journals Research Article LEAVES EXTRACT-BASED BIOGENIC SYNTHESIS OF CUPRIC OXIDE NANOPARTICLES, CHARACTERIZATIONS, AND ANTIMICROBIAL ACTIVITY

2019 ◽  
pp. 288-291
Author(s):  
YASHWANI PRAKASH
Keyword(s):  
Antimicrobial Activity ◽  
Color Change ◽  
Cupric Oxide ◽  
Low Cost ◽  
Copper Acetate ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Acetate Solution ◽  
Zones Of Inhibition ◽  
Cupric Oxide Nanoparticles

Objectives: Tulsi (Ocimum tenuiflorum) leaves extract-based synthesis of cupric oxide nanoparticles (CuONPs), characterizations, and antimicrobial activity. Methods: The small cut leaves were washed with double distilled water and boiled for 30 min. After filtration, the extract was treated with 0.2 M copper acetate solution and the initial color change of this solution indicated formation of copper nanoparticles. This solution was stirred for a specific time, heated and treated with 0.1 M NaOH solution. The formation of CuONPs was confirmed by the development of brownish-black precipitates. Then, CuONPs have been tested for their antibacterial effects by applying well diffusion method against Escherichia coli, Streptococcus mutans, Proteus vulgaris, and Staphylococcus aureus. Results: The biologically synthesized CuONPs have been well characterized by using ultraviolet-visible, Fourier-transform infrared, X-ray powder diffraction, and field-emission scanning electron microscopy techniques and all these analytical methods indicated a successful and efficient formation of CuONPs. After the incubation period, significant zones of inhibition were observed for E. coli, S. mutans, P. vulgaris, and S. aureus. Conclusions: The method was found highly efficient, eco-friendly, and low cost for the synthesis of biologically important CuONPs. The CuONPs have been found an excellent antibacterial agent.

Analysis of the influence of copper on carbohydrate metabolism in carp

2020 ◽  
Vol 2 ◽  
pp. 162-165
Author(s):  
L. Yu. Karpenko ◽  
◽  
P. A. Polistovskaya ◽  
A. B. Balykina ◽  
◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Glucose Concentration ◽  
Serum Amylase ◽  
Amylase Activity ◽  
Copper Acetate ◽  
The Body ◽  
Toxic Effects ◽  
Control Group ◽  
Toxic Substances ◽  
Serum Amylase Activity

The article discusses some aspects of the toxic effects of copper acetate on the body of fish. Heavy metals are highly toxic to aquat-ic organisms due to their ability to accumu-late and transform within the biocenosis of the reservoir. The aim of the study was to study the indicators of carbohydrate metabo-lism in carp after exposure to various con-centrations of copper acetate. The study of the features of carbohydrate metabolism, including the intensity and direction of ex-change can be useful in assessing the re-sponse of fish to toxic effects. During the experiment, 4 groups of fish were formed - 1 control group (10 fish), 3 experimental groups – 10 fish each. Experimental groups of fish were contained in a solution of cop-per acetate (Cu(CH3COO)2) with concentra-tions of 0.01 mg/l, 0.1 mg/l, 1 mg/l (exceeding the MPC of copper for fisheries reservoirs by 10, 100 and 1000 times, re-spectively). Glucose concentration and se-rum amylase activity were studied. Glucose is necessary for supplying energy to various processes occurring inside the body,including reactions to toxic substances. Amylase, however, is an enzyme responsible for catalyzing the breakdown of complex carbohydrates. We found such changes in carbohydrate metabolism in carp as a signifi-cant increase in serum amylase activity by 6.63%, 7.9% and 19.57% when exposed to 10, 100 and 1000 MPC of copper, respec-tively, as well as a significant increase in glucose concentration by 8.54%, 20.28% and 29.7% when exposed to 10, 100 and 1000 MPC of copper, respectively, compared to the control group.

scholarly journals Synthesis and characterization of Cu-MFI catalyst for the direct medium temperature range NO decomposition

2016 ◽  
Vol 34 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Karolina Maduna Valkaj ◽  
Vesna Tomašić ◽  
Andrea Katović ◽  
ElżBieta Bielańska
Keyword(s):  
Ion Exchange ◽  
Active Sites ◽  
Catalytic Properties ◽  
Direct Synthesis ◽  
Copper Acetate ◽  
Nitrogen Adsorption ◽  
No Decomposition ◽  
Medium Temperature ◽  
Exchange Method ◽  
Mfi Zeolites

AbstractIn this study the physico-chemical and catalytic properties of copper bearing MFI zeolites (Cu-MFI) with different Si/Al and Si/Cu ratios were investigated. Two different methods for incorporation of metal ions into the zeolite framework were used: the ion exchange from the solution of copper acetate and the direct hydrothermal synthesis. Direct synthesis of a zeolite in the presence of copper-phosphate complexes was expected to generate more active copper species necessary for the desired reaction than the conventional ion exchange method. Direct decomposition of NO was used as a model reaction, because this reaction still offers a very attractive approach to NOX removal. The catalytic properties of zeolite samples were studied using techniques, such as XRD, SEM, EPR and nitrogen adsorption/desorption measurements at 77 K. Results of the kinetic investigation revealed that both methods are applicable for the preparation of the catalysts with active sites capable of catalyzing the NO decomposition. It was found out that Cu-MFI zeolites obtained through direct synthesis are promising catalysts for NO decomposition, especially at lower reaction temperatures. The efficiency of the catalysts prepared by both methods is compared and discussed.

scholarly journals STUDIES OF HYPERTHYROIDISM

1926 ◽  
Vol 43 (5) ◽  
pp. 703-712 ◽  
Author(s):  
C. C. Speidel
Keyword(s):  
Gall Bladder ◽  
The Body ◽  
Bile Pigment ◽  
Gill Epithelium ◽  
Limited Extent ◽  
Anuran Larvae ◽  
Green Color ◽  
Dark Green ◽  
Pale Green ◽  
Erythrocyte Destruction

Experimental hyperthyroidism in urodele larvæ (Amblystoma) and anuran larvæ (Rana, Bufo, and Hyla) is accompanied by definite changes in bile color. The normal pale green, or pale yellow-green, color of the full gall bladder changes progressively after thyroid administration to a brighter green, then emerald-green, and finally a very dark green. In several hundred observations no exceptions were noted. The bile pigment, biliverdin (and its derivatives), is elaborated from the hemoglobin of worn out erythrocytes. Thyroid administration induces an increased rate of erythrocyte destruction, and this is followed by an increased output of bile pigment. Other minor factors are mentioned which may to a limited extent modify the color of the bile. Erythrocyte destruction occurs largely by enucleation, cytoplasmic segmentation, and fragmentation, and is probably widespread in the body. Many fragments and senile red cells collect in the liver. During the later stages of thyroid treatment the macrophages become conspicuously active. They are especially abundant in the liver, the gut, and the gills. In addition to the hemoglobin eliminated after transformation into bile pigment, some is transported by macrophages through the gut lining, and to a less extent through the involuting gill epithelium, and thus eliminated from the body.

Volume Kinetics of Intravenous Fluid Therapy in the Prehospital Setting

2001 ◽  
Vol 16 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Christer Svensén ◽  
Fredrik Sjöstrand ◽  
Robert G. Hahn
Keyword(s):  
Kinetic Analysis ◽  
Fluid Therapy ◽  
Elimination Rate ◽  
Volume Effect ◽  
The Body ◽  
Control Group ◽  
Trauma Patients ◽  
Acetate Solution ◽  
Crystalloid Fluid ◽  
Ringer’S Acetate Solution

AbstractIntroduction:To study the volume effect of isotonic and hypertonic crystalloid fluid during ambulance transports after mild trauma, a prospective case-control study was initiated, using the ambulance and helicopter transport system in Stockholm.Methods:The hemodilution resulting from intravenous infusion of 1.0 L of Ringer's acetate solution (n = 7) or 250 ml of 7.5% sodium chloride (n = 3) over 30 minutes (min) was measured every 10 min during 1 hour when fluid therapy was instituted at the scene of an accident, or on arrival at the hospital. The dilution was studied by volume kinetic analysis and compared to that of matched, healthy controls who received the same fluid in hospital.Result:The hemodilution at the end of the infusions averaged 7.7% in the trauma patients and 9.1% in the controls, but the dilution was better maintained after trauma. The kinetic analysis showed that the size of the body fluid space expanded by Ringer's solution was 4.6 L and 3.8 L for the trauma and the control patients, respectively, while hypertonic saline expanded a slightly larger space. For both fluids, trauma reduced the elimination rate constant by approximately 30%.Conclusion:Mild trauma prolonged the intravascular persistence of isotonic and hypertonic crystalloid fluid as compared to a control group.

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