scholarly journals Ty21a Live Oral Typhoid Vaccine and Prevention of Paratyphoid Fever Caused by Salmonella enterica Serovar Paratyphi B

2007 ◽  
Vol 45 (Supplement_1) ◽  
pp. S24-S28 ◽  
Author(s):  
Myron M. Levine ◽  
Catterine Ferreccio ◽  
Robert E. Black ◽  
Rosanna Lagos ◽  
Oriana San Martin ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Typhoid Vaccine ◽  
Paratyphi B ◽  
Paratyphoid Fever

scholarly journals Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356

2006 ◽  
Vol 55 (12) ◽  
pp. 1661-1665 ◽  
Author(s):  
Kyung Ho Han ◽  
Seon Young Choi ◽  
Je Hee Lee ◽  
Hyejon Lee ◽  
Eun Hee Shin ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Reference Strain ◽  
Multidrug Resistant ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Functional Product ◽  
Paratyphi B ◽  
In Trans ◽  
Paratyphoid Fever ◽  
Discrimination Method

Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT− (S. Paratyphi B) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT+ (S. Paratyphi B dT+; formerly called Salmonella Java) causes gastroenteritis. S. Java is currently recognized as an emerging problem worldwide. Twelve dT+ S. Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment d-tartrate, reported elsewhere, was validated. The d-tartrate fermenting phenotype of S. Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d-tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT− reference strain was changed to the dT+ phenotype by complementing ORF STM 3356 in trans. The results show that the dT+ phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method.

scholarly journals A Study of Paratyphoid Fever in Bulgarian Children

2021 ◽  
Vol 48 (1) ◽  
pp. 59-62
Author(s):  
M. Pavlova ◽  
E. Alexandrova ◽  
Y. Kalchev ◽  
V. Velev ◽  
M. Murdjeva ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Acute Gastroenteritis ◽  
Salmonella Enterica ◽  
Clinical Course ◽  
Watery Diarrhea ◽  
Pcr Analysis ◽  
Common Cause ◽  
Paratyphi B ◽  
Paratyphoid Fever

Abstract Objective. To study both the molecular discrimination of D-tartrate fermenting and non-fermenting strains of Salmonella enterica subsp. enterica serotype Paratyphi B isolated from patients with paratyphoid fever and the clinical course of this disease. Materials and methods. The isolates examined were from children aged 3 months to 9 years. A total of 33 Salmonella strains were serotyped as Salmonella Paratyphi B, with an antigenic formula based on O- and H- antigens: 1,4, [5], 12: b: 1,2 by Kauffmann–White classification. Results. Multiplex PCR analysis confirmed all tested strains as d-tartrate fermenting (dT+), also referred to as variant Java. Discussion. We found that the most common cause of paratyphoid fever among children in Bulgaria is variant Java Salmonella Paratyphi B. Most children had classic symptoms of acute gastroenteritis – fever, watery diarrhea and vomiting.

scholarly journals Genome-wide analysis provides a deeper understanding of the population structure of the Salmonella enterica serotype Paratyphi B complex in Bangladesh

2021 ◽  
Vol 7 (9) ◽  
Author(s):  
Sadia Isfat Ara Rahman ◽  
Alyce Taylor-Brown ◽  
Farhana Khanam ◽  
Ashraful Islam Khan ◽  
Gal Horesh ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Disease Surveillance ◽  
Sensu Stricto ◽  
Enteric Disease ◽  
Content Type ◽  
Link Type ◽  
Genome Wide ◽  
Wide Range ◽  
Paratyphi B ◽  
Paratyphoid Fever

The Salmonella enterica serotype Paratyphi B complex causes a wide range of diseases, from gastroenteritis to paratyphoid fever, depending on the biotypes Java and sensu stricto. The burden of Paratyphi B biotypes in Bangladesh is still unknown, as these are indistinguishable by Salmonella serotyping. Here, we conducted the first whole-genome sequencing (WGS) study on 79 Salmonella isolates serotyped as Paratyphi B that were collected from 10 nationwide enteric disease surveillance sites in Bangladesh. Placing these in a global genetic context revealed that these are biotype Java, and the addition of these genomes expanded the previously described PG4 clade containing Bangladeshi and UK isolates. Importantly, antimicrobial resistance (AMR) genes were scarce amongst Bangladeshi S. Java isolates, somewhat surprisingly given the widespread availability of antibiotics without prescription. This genomic information provides important insights into the significance of S. Paratyphi B biotypes in enteric disease and their implications for public health.

scholarly journals In Vitro Synergism of Ciprofloxacin and Cefotaxime against Nalidixic Acid-Resistant Salmonella enterica Serotypes Paratyphi A and Paratyphi B

2010 ◽  
Vol 54 (9) ◽  
pp. 3696-3701 ◽  
Author(s):  
Ganesh Prasad Neupane ◽  
Dong-Min Kim ◽  
Sung Hun Kim ◽  
Bok Kwon Lee
Keyword(s):  
Nalidixic Acid ◽  
Salmonella Enterica ◽  
Synergistic Activity ◽  
Bacterial Counts ◽  
Paratyphi B ◽  
Rifampin Resistance ◽  
Resistant Mutants ◽  
Paratyphoid Fever ◽  
Time Kill

ABSTRACT Paratyphoid fever is considered an emerging systemic intracellular infection caused by Salmonella enterica serotypes Paratyphi A, B, and C. We performed in vitro time-kill studies on three clinical isolates of nalidixic acid-resistant Salmonella serotype Paratyphi (NARSP) with different concentrations of ciprofloxacin and cefotaxime to identify combinations of antibiotics with synergistic activity against paratyphoid fever. Furthermore, we identify the frequency of mutations to ciprofloxacin, cefotaxime, and rifampin resistance and also sequenced the gyrA, gyrB, parC, and parE genes to identify the cause of resistance in NARSP. When the activity of ciprofloxacin at 0.75× MIC (0.012 to 0.38 μg/ml) with cefotaxime at the MIC (0.125 to 0.25 μg/ml) against all three NARSP isolates was investigated, synergy was observed at 24 h, and the bacterial counts were reduced by >3 log10 CFU/ml. This synergy was elongated for up to 72 h in two out of three isolates. When ciprofloxacin at 0.75× MIC (0.012 to 0.38 μg/ml) was combined with cefotaxime at 2× MIC (0.25 to 0.50 μg/ml), synergy was prolonged for up to 72 h in all three isolates. Both Salmonella serotype Paratyphi A isolates carried single mutations in codon 83 of the gyrA gene and codon 84 of the parC gene that were responsible for their reduced susceptibility to ciprofloxacin, while no mutations were found in the gyrB or parE gene. The ciprofloxacin-plus-cefotaxime regimen was very effective in reducing the bacterial counts at 24 h for all three isolates, and this combination therapy may be helpful in reducing the chance of the emergence of fluoroquinolone-resistant mutants in patients with severe paratyphoid fever.

scholarly journals Classification of Salmonella enterica of the (Para-)Typhoid Fever Group by Fourier-Transform Infrared (FTIR) Spectroscopy

2021 ◽  
Vol 9 (4) ◽  
pp. 853
Author(s):  
Miriam Cordovana ◽  
Norman Mauder ◽  
Markus Kostrzewa ◽  
Andreas Wille ◽  
Sandra Rojak ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Salmonella Typhi ◽  
Food Samples ◽  
Bacterial Typing ◽  
Suitable Alternative ◽  
Promising Tool ◽  
Resource Limited ◽  
Paratyphi B ◽  
Paratyphoid Fever

Typhoidal and para-typhoidal Salmonella are major causes of bacteraemia in resource-limited countries. Diagnostic alternatives to laborious and resource-demanding serotyping are essential. Fourier transform infrared spectroscopy (FTIRS) is a rapidly developing and simple bacterial typing technology. In this study, we assessed the discriminatory power of the FTIRS-based IR Biotyper (Bruker Daltonik GmbH, Bremen, Germany), for the rapid and reliable identification of biochemically confirmed typhoid and paratyphoid fever-associated Salmonella isolates. In total, 359 isolates, comprising 30 S. Typhi, 23 S. Paratyphi A, 23 S. Paratyphi B, and 7 S. Paratyphi C, respectively and other phylogenetically closely related Salmonella serovars belonging to the serogroups O:2, O:4, O:7 and O:9 were tested. The strains were derived from clinical, environmental and food samples collected at different European sites. Applying artificial neural networks, specific automated classifiers were built to discriminate typhoidal serovars from non-typhoidal serovars within each of the four serogroups. The accuracy of the classifiers was 99.9%, 87.0%, 99.5% and 99.0% for Salmonella Typhi, Salmonella Paratyphi A, B and Salmonella Paratyphi C, respectively. The IR Biotyper is a promising tool for fast and reliable detection of typhoidal Salmonella. Hence, IR biotyping may serve as a suitable alternative to conventional approaches for surveillance and diagnostic purposes.

scholarly journals Evolution and Population Structure of Salmonella enterica Serovar Newport

2010 ◽  
Vol 192 (24) ◽  
pp. 6465-6476 ◽  
Author(s):  
Vartul Sangal ◽  
Heather Harbottle ◽  
Camila J. Mazzoni ◽  
Reiner Helmuth ◽  
Beatriz Guerra ◽  
...  
Keyword(s):  
Population Structure ◽  
North America ◽  
Salmonella Enterica ◽  
Health Concern ◽  
Global Public Health ◽  
Multiple Sequence ◽  
Monophyletic Lineage ◽  
Population Structures ◽  
Paratyphi B ◽  
Multiple Drugs

ABSTRACT Salmonellosis caused by Salmonella enterica serovar Newport is a major global public health concern, particularly because S. Newport isolates that are resistant to multiple drugs (MDR), including third-generation cephalosporins (MDR-AmpC phenotype), have been commonly isolated from food animals. We analyzed 384 S. Newport isolates from various sources by a multilocus sequence typing (MLST) scheme to study the evolution and population structure of the serovar. These were compared to the population structure of S. enterica serovars Enteritidis, Kentucky, Paratyphi B, and Typhimurium. Our S. Newport collection fell into three lineages, Newport-I, Newport-II, and Newport-III, each of which contained multiple sequence types (STs). Newport-I has only a few STs, unlike Newport-II or Newport-III, and has possibly emerged recently. Newport-I is more prevalent among humans in Europe than in North America, whereas Newport-II is preferentially associated with animals. Two STs of Newport-II encompassed all MDR-AmpC isolates, suggesting recent global spread after the acquisition of the bla CMY-2 gene. In contrast, most Newport-III isolates were from humans in North America and were pansusceptible to antibiotics. Newport was intermediate in population structure to the other serovars, which varied from a single monophyletic lineage in S. Enteritidis or S. Typhimurium to four discrete lineages within S. Paratyphi B. Both mutation and homologous recombination are responsible for diversification within each of these lineages, but the relative frequencies differed with the lineage. We conclude that serovars of S. enterica provide a variety of different population structures.

scholarly journals Improving Our Understanding of Salmonella enterica Serovar Paratyphi B through the Engineering and Testing of a Live Attenuated Vaccine Strain

mSphere ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Ellen E. Higginson ◽  
Girish Ramachandran ◽  
Tracy H. Hazen ◽  
Dane A. Kania ◽  
David A. Rasko ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Salmonella Enterica ◽  
Enteric Fever ◽  
Sensu Stricto ◽  
Live Attenuated Vaccine ◽  
Content Type ◽  
Heterologous Challenge ◽  
Paratyphi B ◽  
Homologous Challenge ◽  
Ideal Vaccine

ABSTRACT Enteric fever is caused by three Salmonella enterica serovars: Typhi, Paratyphi A, and Paratyphi B sensu stricto. Although vaccines against two of these serovars are licensed (Typhi) or in clinical development (Paratyphi A), as yet there are no candidates for S. Paratyphi B. To gain genomic insight into these serovars, we sequenced 38 enteric fever-associated strains from Chile and compared these with reference genomes. Each of the serovars was separated genomically based on the core genome. Genomic comparisons identified loci that were aberrant between serovars Paratyphi B sensu stricto and Paratyphi B Java, which is typically associated with gastroenteritis; however, the majority of these were annotated as hypothetical or phage related and thus were not ideal vaccine candidates. With the genomic information in hand, we engineered a live attenuated S. Paratyphi B sensu stricto vaccine strain, CVD 2005, which was capable of protecting mice from both homologous challenge and heterologous challenge with S. Paratyphi B Java. These findings extend our understanding of S. Paratyphi B and provide a viable vaccine option for inclusion in a trivalent live attenuated enteric fever vaccine formulation. IMPORTANCE We developed a live attenuated Salmonella enterica serovar Paratyphi B vaccine that conferred protection in mice against challenge with S. Paratyphi B sensu stricto and S. Paratyphi B Java, which are the causes of enteric fever and gastroenteritis, respectively. Currently, the incidence of invasive S. Paratyphi B sensu stricto infections is low; however, the development of new conjugate vaccines against other enteric fever serovars could lead to the emergence of S. Paratyphi B to fill the niche left by these other pathogens. As such, an effective S. Paratyphi B vaccine would be a useful tool in the armamentarium against Salmonella infections. Comparative genomics confirmed the serovar-specific groupings of these isolates and revealed that there are a limited number of genetic differences between the sensu stricto and Java strains, which are mostly hypothetical and phage-encoded proteins. The observed level of genomic similarity likely explains why we observe some cross-protection.

scholarly journals A PLAUSIBLE COMMUNITY INDUCED SALMONELLA ENTERICA SEROTYPE PARATYPHI B INFECTION CAUSING INFANTILE MENINGITIS: A CASE REPORT

2012 ◽  
Vol 02 (04) ◽  
pp. 63-64
Author(s):  
Ganesh H. R. ◽  
Vimal Kumar Karnaker ◽  
Sanjeev H. ◽  
Rekha Rai ◽  
Asha Pai K. B. ◽  
...  
Keyword(s):  
Case Report ◽  
Salmonella Enterica ◽  
Blood Cultures ◽  
Female Infant ◽  
Pyogenic Meningitis ◽  
Paratyphi B

AbstractA female infant admitted with pyogenic meningitis due to Salmonella enterica serotype Paratyphi B. The organism was isolated from CSF and blood cultures.

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