Fluorescence enhancement from nano-gap embedded plasmonic gratings by a novel fabrication technique with HD-DVD

2012 ◽  
Vol 23 (49) ◽  
pp. 495201 ◽  
Author(s):  
K Bhatnagar ◽  
A Pathak ◽  
D Menke ◽  
P V Cornish ◽  
K Gangopadhyay ◽  
...  
Keyword(s):  
Fluorescence Enhancement ◽  
Fabrication Technique ◽  
Plasmonic Gratings

UHV-STM studies of silicon nano-pyramid growth on silicon surface at high temperature

1992 ◽  
Vol 50 (2) ◽  
pp. 1144-1145
Author(s):  
T. Sato ◽  
S. Kitamura ◽  
T. Sueyoshl ◽  
M. Iwatukl ◽  
C. Nielsen
Keyword(s):  
High Temperature ◽  
Relaxation Process ◽  
Thermal Effect ◽  
Bias Voltage ◽  
Silicon Surface ◽  
Growth Process ◽  
Tunneling Current ◽  
Fabrication Technique ◽  
Nano Fabrication ◽  
Electromigration Effect

Recently, the growth process and relaxation process of crystalline structures were studied by observing a SI nano-pyramid which was built on a Si surface with a UHV-STM. A UHV-STM (JEOL JSTM-4000×V) was used for studying a heated specimen, and the specimen was kept at high temperature during observation. In this study, the nano-fabrication technique utilizing the electromigration effect between the STM tip and the specimen was applied. We observed Si atoms migrated towords the tip on a high temperature Si surface.Clean surfaces of Si(lll)7×7 and Si(001)2×l were prepared In the UHV-STM at a temperature of approximately 600 °C. A Si nano-pyramid was built on the Si surface at a tunneling current of l0nA and a specimen bias voltage of approximately 0V in both polarities. During the formation of the pyramid, Images could not be observed because the tip was stopped on the sample. After the formation was completed, the pyramid Image was observed with the same tip. After Imaging was started again, the relaxation process of the pyramid started due to thermal effect.

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

The Difference in Sensing Properties Towards Anions between Two Pyridinium Amide-Based Receptors with a Subtle Change of H-Bond Position

2020 ◽  
Vol 17 (6) ◽  
pp. 472-478
Author(s):  
Wei-tao Gong ◽  
Wei-dong Qu ◽  
Guiling Ning
Keyword(s):  
Fluorescence Enhancement ◽  
Small Difference ◽  
Fluorescence Emission ◽  
Subtle Change ◽  
Sensing Properties ◽  
Naked Eye ◽  
Recognition Ability ◽  
The Difference ◽  
L1 And L2

Two pyridinium amide-based receptors L1 and L2 with a small difference of H-bond position of the amide have been synthesized and characterized. Interestingly, they exhibited a huge difference in sensing towards AcO- and H2PO4 -, respectively. Receptor L1 was found to be ‘naked-eye’ selective for AcO- anions, while receptor L2 showed clear fluorescence enhancement selective to H2PO4 - anion. The recognition ability has been established by fluorescence emission, UV-vis spectra, and 1HNMR titration.

Semiautomated microfluorometric instrument and reagent system for monitoring disease-related immunosuppression.

1982 ◽  
Vol 28 (9) ◽  
pp. 1887-1893 ◽  
Author(s):  
D F Ranney ◽  
A J Quattrone
Keyword(s):  
Immune Response ◽  
Dna Content ◽  
Peripheral Blood ◽  
Fluorescence Enhancement ◽  
Photon Counting ◽  
Peripheral Blood Lymphocytes ◽  
Response Modulation ◽  
Blood Lymphocytes ◽  
Complex Test ◽  
Immune Response Modulation

Abstract Several common metabolites and drugs in the serum in of patients with inflammatory, infectious, autoimmune, immunodeficient, neoplastic, and toxicant-induced diseases can produce artifactual suppression of the [methyl-3H]-thymidine assay, which is widely used to evaluate lymphocyte responsiveness. We have developed a sensitive, semiautomated, fluorescence-enhancement assay in which true immunosuppressors are measured in the presence of absence of such interfering substances. Peripheral blood lymphocytes are activated with mitogens in standard microtiter culture trays. Changes in lymphocyte DNA content are quantified with a reagent formulation containing mithramycin, the fluorescence of which is enhanced on binding to DNA in the presence of MgCl2. We solubilize cells within the intact microtiter tray by using an automated, inverted "Array Sonicator," and measure fluorescence with an automated, photon-counting fluorometer. With this system, immune response modulation can be accurately assessed in the presence of patients' sera and other complex test substances (e.g., supernates from hybridomas, fermentation vats, viral preparations, and macrophage cultures.

Controlled Fluorescence Enhancement of DNA-Binding Dye Through Chain Length Match between Oligoguanine and TOTO

2021 ◽  
Vol 125 (2) ◽  
pp. 518-527
Author(s):  
Fangjia Fu ◽  
Kang Liao ◽  
Ziteng Liu ◽  
Daocheng Hong ◽  
Haitang Yang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Chain Length ◽  
Fluorescence Enhancement

Sensitive and specific detection of peroxynitrite and in-vivo imaging of inflammation by a "simple" AIE bioprobe

2021 ◽  
Author(s):  
Huilin Xie ◽  
Jingtian Zhang ◽  
Chao Chen ◽  
Feiyi Sun ◽  
Haixiang Liu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescence Enhancement ◽  
Specific Detection ◽  
Aggregation Induced Emission

A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO−) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage...

scholarly journals One-Shot Fabrication of Polymeric Hollow Microneedles by Standard Photolithography

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 520 ◽  
Author(s):  
Principia Dardano ◽  
Selene De Martino ◽  
Mario Battisti ◽  
Bruno Miranda ◽  
Ilaria Rea ◽  
...  
Keyword(s):  
Light Propagation ◽  
World Market ◽  
Emerging Technology ◽  
Standard Formulation ◽  
Proof Of Concept ◽  
Fabrication Technique ◽  
The World ◽  
Small Doses ◽  
Approved Drugs ◽  
Hollow Microneedles

Microneedles (MNs) are an emerging technology in pharmaceutics and biomedicine, and are ready to be commercialized in the world market. However, solid microneedles only allow small doses and time-limited administration rates. Moreover, some well-known and already approved drugs need to be re-formulated when supplied by MNs. Instead, hollow microneedles (HMNs) allow for rapid, painless self-administrable microinjection of drugs in their standard formulation. Furthermore, body fluids can be easily extracted for analysis by a reverse use of HMNs, thus making them perfect for sensing issues and theranostics applications. The fabrication of HMNs usually requires several many-step processes, increasing the costs and consequently decreasing the commercial interest. Photolithography is a well-known fabrication technique in microelectronics and microfluidics that fabricates MNs. In this paper, authors show a proof of concept of a patented, easy and one-shot fabrication of two kinds of HMNs: (1) Symmetric HMNs with a “volcano” shape, made by using a photolithographic mask with an array of transparent symmetric rings; and (2) asymmetric HMNs with an oblique aperture, like standard hypodermic steel needles, made by using an array of transparent asymmetric rings, defined by two circles, which centers are slightly mismatched. Simulation of light propagation, fabrication process, and preliminary results on ink microinjection are presented.

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