Dynamic light scattering detection of silver nanoparticles, food pathogen bacteria and their bactericidal interactions

ABSTRACT This paper reports citrate-stabilized silver nanoparticles (AgNPs) synthesized by nitrate ion chemical reduction with sodium borohydride, at different pHs (2–9). The AgNPs synthesized by this method exhibited size distribution from 5 to 249 nm, depending on pH, as determined by dynamic light scattering, and morphology spherical, as determined by transmission electron microscopy. In pH range 3–7 occurred aggregation of the nanoparticles. The size distribution depending on pH was determined by dynamic light scattering. The zeta potential was determined, and the colloidal stability was correlated with nanoparticles aggregation at different pHs. The size-dependent antimicrobial activity was evaluated for two solutions, wherein both samples exhibited antimicrobial activity, although the smallest AgNPs without agglomeration have enhanced antimicrobial properties.

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Optimization of Process Parameters in Bioreduction of Silver Nanoparticles by Pycnoporus sanguineus

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.626.95 ◽

2012 ◽

Vol 626 ◽

pp. 95-98

Author(s):

Yen San Chan ◽

Mashitah Mat Don

Keyword(s):

Silver Nanoparticles ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Culture Supernatant ◽

Inoculum Size ◽

Medical Applications ◽

Pycnoporus Sanguineus ◽

Pharmaceutical Applications ◽

Optimization Of Process Parameters ◽

Biological And Medical Applications

Controlling the size of nanomaterials are attracting great interest in the research on scientific and technological applications because of their unit properties for achieving specific processes especially in biological and medical applications. Microbial assisted biosynthesis of nanoparticles is of growing potential in the area of bionanotechnology compared to chemical synthesis when dealing with medical and pharmaceutical applications. A simple and effective approach for AgNPs synthesis by Pycnoporus sanguineus was demonstrated and the effect of production mode on controlling size of AgNPs produced was studied. Culture supernatant of Pycnoporus sanguineus was used to synthesis AgNPs of nanosize. One factor at a time (OFAT) method was employed to perform optimization on process parameter such as inoculum size and AgNO3 concentration. The morphology, uniformity and concentration of AgNPs were investigated using dynamic light scattering (DLS) zetasizer and atomic absorption spectroscopy. It was observed that increase in inoculum size leads to decrease in size of AgNPs and increase of AgNPs concentration. Hence, it can be deduced that optimizing the bioprocess parameters led to superior control of AgNPs size.

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Interactive influence of extracellular polymeric substances (EPS) and electrolytes on the colloidal stability of silver nanoparticles

Environmental Science Nano ◽

10.1039/c9en00861f ◽

2020 ◽

Vol 7 (1) ◽

pp. 186-197 ◽

Cited By ~ 2

Author(s):

Ishara Fernando ◽

Dan Lu ◽

Yan Zhou

Keyword(s):

Silver Nanoparticles ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Electrophoretic Mobility ◽

Extracellular Polymeric Substances ◽

Colloidal Stability ◽

Time Resolved

The colloidal stability of silver nanoparticles (AgNPs) was evaluated using time-resolved dynamic light scattering, electrophoretic mobility and dissolved Ag concentration in the presence of electrolytes and extracellular polymeric substances (EPS).

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Colorimetric and dynamic light scattering detection of DNA sequences by using positively charged gold nanospheres: a comparative study with gold nanorods

Nanotechnology ◽

10.1088/0957-4484/22/28/285501 ◽

2011 ◽

Vol 22 (28) ◽

pp. 285501 ◽

Cited By ~ 21

Author(s):

T E Pylaev ◽

V A Khanadeev ◽

B N Khlebtsov ◽

L A Dykman ◽

V A Bogatyrev ◽

...

Keyword(s):

Light Scattering ◽

Dynamic Light Scattering ◽

Comparative Study ◽

Gold Nanorods ◽

Dna Sequences ◽

Gold Nanospheres ◽

Light Scattering Detection ◽

Positively Charged

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Enhanced Antibacterial and Antioxidant Properties of Chitosan Films Blended with Gallic Acid and Incorporated with Thymol Silver Nanoparticles

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i6-s.5130 ◽

2021 ◽

Vol 11 (6-S) ◽

pp. 53-69

Author(s):

Bipin Lade ◽

Akash Kamdi ◽

Arti Shanware

Keyword(s):

Antimicrobial Activity ◽

Wound Healing ◽

Silver Nanoparticles ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Gallic Acid ◽

Antioxidant Properties ◽

Surface Charges ◽

Vis Spectroscopy ◽

Chitosan Films

In the present study, an attempt has been made to build and evaluate Chitosan+Glycerol/Gallic acid/Thymol-silver nanoparticles or chitosan blended (C+G/GA/T-SNPs) film to significantly improve antioxidant and antibacterial activity for accelerated wound healing. Methanolic Gallic acid is used for the first time in antibacterial chitosan control (C+G) films. All developed films, compounds was Thymol and Gallic acid and their synthesized Thymol silver nanoparticles (T-SNPs) and Gallic acid silver nanoparticles (GA-SNPs) were characterized by Fourier-transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-Ray diffraction (XRD), Zeta potential (ZP), Dynamic light scattering (DLS), and UV-Vis spectroscopy. T-SNPs and GA-SNPs are rod and spherical in shape and were sufficient to reduce, capped, and stabilize. T-SNPs and GA-SNPs were measured Dynamic Light scattering and found to be 123.2 nm and 121.1 nm with surface charges of -19.7 and -20.3 respectively. The incorporation of methanolic Gallic acid and T-SNPs into chitosan films, as predicted, effectively enhanced antioxidant and antimicrobial activity. The antimicrobial activity of Thymol, T-SNPs and C+G/GA/T-SNPs film showed more zone of inhibition than Gallic acid, GA-SNPs and C+G film. The elasticity, texture and folding endurance of the C+G film and C+G/GA/T-SNPs films have been substantially improved. The ecological quality of the generated C+G and C+G/GA/T-SNPs film was determined by the assessment of soil degradation and water degradation parameters. These findings lead to the conclusion that the C+G/GA/T-SNPs film produced with Gallic acid and T-SNPs can improve wound healing. Keywords: Chitosan, Gallic Acid, Thymol Silver Nanoparticles, Antibacterial and Antioxidant Properties.

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How Temperature and Photosynthetically Active Radiation Impact the Green Synthesis of Silver Nanoparticles Using Aqueous Extracts of Mentha Leaves?

Biointerface Research in Applied Chemistry ◽

10.33263/briac124.45974602 ◽

2021 ◽

Vol 12 (4) ◽

pp. 4597-4602

Keyword(s):

Silver Nanoparticles ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Green Synthesis ◽

Photosynthetically Active Radiation ◽

Hydrodynamic Diameter ◽

Synthesis Methods ◽

Aqueous Extracts ◽

Polydisperse Suspension ◽

Active Radiation

Green synthesis highlights sustainable methods to produce silver nanoparticles (AgNPs). Here, extracts from fresh and lyophilized Mentha leaves produced AgNPs when performing reactions in the dark at 25ºC or 75ºC; also under photosynthetically active radiation (PAR) at 25ºC aiming to compare hydrothermal and photochemical methods. AgNPs formation was spectrophotometrically monitored and characterized by dynamic light scattering (DLS) and Zeta potential (ZP). The most polydisperse AgNPs suspension was synthesized at 25ºC (dark), presenting polydispersity index (PdI) of 0.574±0.061, and exhibited the lowest hydrodynamic diameter (HD) of 44.34±1.60 nm. In contrast, the highest HD was 80.15±2.88 nm to AgNPs produced at 25ºC with PAR which exhibited ZP of -27.8±0.7 mV. The lowest polydisperse suspension was produced at 75ºC (dark), presenting PdI of 0.369±0.009 and ZP of -12.8±0.6 mV. Concluding, we compared reliable green synthesis’ methods to determine which would efficiently produce AgNPs using Mentha leaves.

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Creating extended antimicrobial property in paper by means of Ag and nanohybrids of montmorillonite (MMT)

Holzforschung ◽

10.1515/hf-2016-0195 ◽

2017 ◽

Vol 71 (5) ◽

pp. 445-454 ◽

Cited By ~ 2

Author(s):

Elyas Afra ◽

Parvaneh Narchin

Keyword(s):

Silver Nanoparticles ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Antibacterial Property ◽

Antimicrobial Property ◽

Antibacterial Properties ◽

Silver Particles ◽

Sem Images ◽

Nanometer Range ◽

Great Accumulation

Abstract Silver-clay nanohybrids based on silver nanoparticles (AgNPs) with montmorillonit (MMT) and milled MMT, shortly AgMMTnano and AgMMTmilled, have been prepared to improve the performance of antibacterial properties of paper. The results obtained by dynamic light scattering indicated the presence of silver particles in the nanometer range, which have a great accumulation tendency. The results of AAS and ICP-AES tests showed that nanosilver amount in the AgMMTmilled was doubled compared to the AgMMTnano, but according to the EDX results, the amount of nanosilver in paper treated with the former was less than in the latter. FE-SEM images showed that the dimensions of deposited nanosilver on the surface of clay were smaller than in case of AgNPs alone. Papers treated with nanohybrids had better antibacterial properties compared to that prepared by naked nanosilver. It was also found that the antibacterial property of papers treated with AgMMTnano were more stable during three-stage tests compared to those with AgMMTmilled. Nanohybrids with Ag conc. more than 15 ppm, especially hybrids with Ag conc. of 50 ppm, result in a significant improvement in antibacterial properties of paper. It can be concluded that silver-clay nanohybrid with a 25-ppm concentration of nanosilver could be the best and most economic antibacterial component.

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