scholarly journals Enhanced Antibacterial and Antioxidant Properties of Chitosan Films Blended with Gallic Acid and Incorporated with Thymol Silver Nanoparticles

2021 ◽  
Vol 11 (6-S) ◽  
pp. 53-69
Author(s):  
Bipin Lade ◽  
Akash Kamdi ◽  
Arti Shanware
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Surface Charges ◽  
Vis Spectroscopy ◽  
Chitosan Films

In the present study, an attempt has been made to build and evaluate Chitosan+Glycerol/Gallic acid/Thymol-silver nanoparticles or chitosan blended (C+G/GA/T-SNPs) film to significantly improve antioxidant and antibacterial activity for accelerated wound healing. Methanolic Gallic acid is used for the first time in antibacterial chitosan control (C+G) films. All developed films, compounds was Thymol and Gallic acid and their synthesized Thymol silver nanoparticles (T-SNPs) and Gallic acid silver nanoparticles (GA-SNPs) were characterized by Fourier-transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-Ray diffraction (XRD), Zeta potential (ZP), Dynamic light scattering (DLS), and UV-Vis spectroscopy. T-SNPs and GA-SNPs are rod and spherical in shape and were sufficient to reduce, capped, and stabilize. T-SNPs and GA-SNPs were measured Dynamic Light scattering and found to be 123.2 nm and 121.1 nm with surface charges of -19.7 and -20.3 respectively. The incorporation of methanolic Gallic acid and T-SNPs into chitosan films, as predicted, effectively enhanced antioxidant and antimicrobial activity. The antimicrobial activity of Thymol, T-SNPs and C+G/GA/T-SNPs film showed more zone of inhibition than Gallic acid, GA-SNPs and C+G film. The elasticity, texture and folding endurance of the C+G film and C+G/GA/T-SNPs films have been substantially improved. The ecological quality of the generated C+G and C+G/GA/T-SNPs film was determined by the assessment of soil degradation and water degradation parameters. These findings lead to the conclusion that the C+G/GA/T-SNPs film produced with Gallic acid and T-SNPs can improve wound healing. Keywords: Chitosan, Gallic Acid, Thymol Silver Nanoparticles, Antibacterial and Antioxidant Properties.

scholarly journals pH effect on the synthesis of different size silver nanoparticles evaluated by DLS and their size-dependent antimicrobial activity

2020 ◽  
Vol 25 (4) ◽  
Author(s):  
Leiriana Aparecida Pinto Gontijo ◽  
Ellen Raphael ◽  
Daniela Pereira Santos Ferrari ◽  
Jefferson Luis Ferrari ◽  
Juliana Pereira Lyon ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Size Distribution ◽  
Colloidal Stability ◽  
Chemical Reduction ◽  
Ph Effect ◽  
Antimicrobial Properties ◽  
Size Dependent

ABSTRACT This paper reports citrate-stabilized silver nanoparticles (AgNPs) synthesized by nitrate ion chemical reduction with sodium borohydride, at different pHs (2–9). The AgNPs synthesized by this method exhibited size distribution from 5 to 249 nm, depending on pH, as determined by dynamic light scattering, and morphology spherical, as determined by transmission electron microscopy. In pH range 3–7 occurred aggregation of the nanoparticles. The size distribution depending on pH was determined by dynamic light scattering. The zeta potential was determined, and the colloidal stability was correlated with nanoparticles aggregation at different pHs. The size-dependent antimicrobial activity was evaluated for two solutions, wherein both samples exhibited antimicrobial activity, although the smallest AgNPs without agglomeration have enhanced antimicrobial properties.

scholarly journals Detection Limits of DLS and UV-Vis Spectroscopy in Characterization of Polydisperse Nanoparticles Colloids

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Emilia Tomaszewska ◽  
Katarzyna Soliwoda ◽  
Kinga Kadziola ◽  
Beata Tkacz-Szczesna ◽  
Grzegorz Celichowski ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Point Of View ◽  
Spectroscopy Analysis ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
The One

Dynamic light scattering is a method that depends on the interaction of light with particles. This method can be used for measurements of narrow particle size distributions especially in the range of 2–500 nm. Sample polydispersity can distort the results, and we could not see the real populations of particles because big particles presented in the sample can screen smaller ones. Although the theory and mathematical basics of DLS technique are already well known, little has been done to determine its limits experimentally. The size and size distribution of artificially prepared polydisperse silver nanoparticles (NPs) colloids were studied using dynamic light scattering (DLS) and ultraviolet-visible (UV-Vis) spectroscopy. Polydisperse colloids were prepared based on the mixture of chemically synthesized monodisperse colloids well characterized by atomic force microscopy (AFM), transmission electron microscopy (TEM), DLS, and UV-Vis spectroscopy. Analysis of the DLS results obtained for polydisperse colloids reveals that several percent of the volume content of bigger NPs could screen completely the presence of smaller ones. The presented results could be extremely important from nanoparticles metrology point of view and should help to understand experimental data especially for the one who works with DLS and/or UV-Vis only.

Dynamic light scattering detection of silver nanoparticles, food pathogen bacteria and their bactericidal interactions

2021 ◽  
Vol 18 (8) ◽  
pp. 086002
Author(s):  
A A Nastulyavichus ◽  
S I Kudryashov ◽  
E R Tolordava ◽  
L F Khaertdinova ◽  
Yu K Yushina ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Light Scattering Detection ◽  
Food Pathogen

Ampicillin-augmented silver nanoparticles for synergistic antimicrobial response: A promising therapeutic approach

2021 ◽  
Vol 22 ◽  
Author(s):  
Kashan Khan ◽  
Mohd Aamir Qureshi ◽  
Ameer Azam ◽  
Moinuddin ◽  
Javed Musarrat ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Bacterial Infections ◽  
Colloidal Stability ◽  
Resistant Bacteria ◽  
Antimicrobial Drugs ◽  
Vis Spectroscopy ◽  
Microbial Infections ◽  
Ft Ir

Aims: Globally Scientists are working to find more efficient antimicrobial drugs to treat microbial infections and kill drug-resistant bacteria. Background: Despite the availability of numerous antimicrobial drugs bacterial infections still poses a serious threat to global health. Due to a constant decline in the effectiveness of antibiotics owing to their repeated exposure as well as shortlasting antimicrobial activity, led to the demand for developing novel therapeutic agents capable of controlling microbial infections. Objective: In this study, we report antimicrobial activity of chemically synthesized silver nanoparticles (cAgNPs) augmented with ampicillin (amp) in order to increase antimicrobial response against Escherichia coli (gram –ve), Staphylococcus aureus (gram +ve) and Streptococcus mutans (gram +ve). Methods: Nanostructure, colloidal stability, morphology and size of cAgNPs before and after functionalization were explored by UV-vis spectroscopy, FT-IR, zeta potential and TEM. The formation and functionalization of cAgNPs was confirmed from UV-vis spectroscopy and FT-IR patterns. From TEM the average sizes of cAgNPs and cAgNP-amp were found to be 13 and 7.8 nm respectively, and change in colloidal stability after augmentation was confirmed from zeta potential values. The antimicrobial efficacies of cAgNP-amp and cAgNPs against E. coli S. aureus and S. mutans were studied by determining minimum inhibitory concentrations (MICs), zone of inhibition, assessment of viable and non-viable bacterial cells and quantitative assessment of biofilm. Results & Discussion: Our results revealed cAgNP-amp to be highly bactericidal compared to cAgNPs or amp alone. The nano-toxicity studies indicated cAgNP-amp to be less toxic compared to cAgNPs alone. Results: This study manifested that cAgNPs show synergistic antimicrobial effect when they get functionalized with amp suggesting their application in curing long-term bacterial infections.

A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds

2020 ◽  
Vol 8 (32) ◽  
pp. 7197-7212 ◽  
Author(s):  
Zhiyong Qian ◽  
Yating Bai ◽  
Jin Zhou ◽  
Linhao Li ◽  
Jing Na ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Silk Fibroin ◽  
Broad Spectrum ◽  
Electrolyte Balance ◽  
Infected Wounds ◽  
Multifunctional Properties

The CTS-SF/SA/Ag-Exo dressing possessed multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.

Green Synthesis of Silver Nanostructures Using Aqueous Extract of Dracocephalum kotschyi and Evaluation of Antioxidant Properties of Herbal Extracts and Antibacterial Feature of Green- Synthesized Nanostructures

2019 ◽  
Vol 13 (3) ◽  
pp. 223-231
Author(s):  
Zahra Goli ◽  
Cobra Izanloo
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Size Distribution ◽  
Aqueous Extract ◽  
Nitrate Solution ◽  
Antioxidant Properties ◽  
Herbal Extract ◽  
Silver Nanostructures ◽  
Dracocephalum Kotschyi ◽  
Vis Spectroscopy

Background: Silver nanoparticles have a profound role in the field of high sensitivity biomolecular detection, catalysis, biosensors and medicine. In the present study, aqueous extract of Dracocephalum kotschyi has been used for the synthesis of silver nanoparticles. Objective: In this study, we evaluated the antioxidant features and the possibility of biosynthesis of AgNPs using an aqueous extract of Dracocephalum kotschyi and also evaluated the antibacterial activities of the synthesized nanoparticles. Methods: An eco-friendly and cost-effective protocol for the synthesis of Ag nanoparticles by utilizing a renewable natural resource, aqueous solution of Dracocephalum kotschyi, was proposed. Synthesized nanoparticles were characterized by UV–Vis spectroscopy, SEM, EDS, and XRD pattern. Results: At first, the extract of Dracocephalum kotschyi was assessed to determine and confirm the presence of an antioxidant feature. Resuscitation of one mM silver nitrate solution was carried out by the herbal extract. The solution containing AgNPs obtained from green synthesis had a maximum optical density at 225 nm. In addition, the presence of AgNPs was approved by energy-dispersive X-ray spectroscopy (EDS). Images of the scanning electron microscope demonstrated that the synthesized AgNPs had the shape of rods and the size distribution of 48-51 nm. One of the benefits of this method is a uniform size distribution. Moreover, the effects of reaction time and concentration of the herbal extract were assessed by ultraviolet-visible (UV-Vis) spectroscopy. In the end, we assessed the antibacterial impact of the synthesized AgNPs against some pathogenic bacterial strains. According to the results, the produced nanostructures had a proper impact on two bacteria of Escherichia coli and Staphylococcus aureus. Conclusion: According to the results of the present study, Dracocephalum kotschyi can be a suitable compound for the synthesis of nanostructures due to its indigenous cultivation and great medicinal properties.

Quantitative investigations of the aggregation behaviour of hydrophobic anilino squaraine dyes through UV/vis spectroscopy and dynamic light scattering

1999 ◽  
Vol 77 (5-6) ◽  
pp. 903-912 ◽  
Author(s):  
James Wojtyk ◽  
Andrew McKerrow ◽  
Peter Kazmaier ◽  
Erwin Buncel
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Particle Diameter ◽  
Water Medium ◽  
Thermodynamic Control ◽  
Squaraine Dyes ◽  
Water Solvent ◽  
Aggregation Behaviour ◽  
Vis Spectroscopy ◽  
J Aggregates

In continuing studies of the aggregation behaviour of squaraine dyes in DMSO-water mixtures, we have examined a series of symmetrical anilino-based squaraines with increasing N-alkyl chain length (n-butyl, n-octyl, and n-dodecyl). The aggregation behaviour was assessed through UV/vis spectroscopic and Dynamic Light Scattering (DLS) studies with quantitative interpretation based on exciton theory. This class of N-alkyl squaraines forms two distinct solution aggregates, Type J and Type H, depending on the composition of the DMSO-water medium. DLS measurement of the aggregates in the Type J and Type H morphologies showed that (i) the particle diameter increased with increasing hydrophobicity; (ii) DMSO has a large effect on the aggregate size; (iii) Type J aggregates are of equal dimensions with Type H aggregates. Moreover, even though as the DMSO-water solvent composition is varied the UV/vis spectra of Type H and Type J aggregates remained unchanged, the number of molecules comprising each aggregate varies widely. It is proposed that the dynamic conversion (J →> H) results from intramolecular reorganization of individual molecules within the aggregate. Quantification of aggregation through DLS has further developed the "kinetic" versus "thermodynamic" control model of dye aggregation.Key words: squaraine, dynamic light scattering, DMSO-water mixtures, self-assembly, kinetic/thermodynamic control.

Ultrasound-assisted green synthesis of silver nanoparticles and their incorporation in antibacterial cellulose packaging

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Vladimir Popov ◽  
Ivaylo Hinkov ◽  
Svetlomir Diankov ◽  
Maria Karsheva ◽  
Yordan Handzhiyski
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Surface Area ◽  
Reduction Method ◽  
Cost Effective ◽  
Antimicrobial Efficiency ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Ultrasound Assisted ◽  
Strong Antimicrobial Activity

AbstractThe antimicrobial activity of nanoparticles (NPs) depends of the surface area in contact with microorganisms. The large surface area of the nanoparticles enhances their interaction with the microbes. In this work, a green, simple, rapid, and efficient ultrasound-assisted reduction method for silver nanoparticles (AgNP) synthesis is presented. For the synthesis, an aqueous solution of silver nitrate, ethanol, and ammonia was used. The adopted method can be easily implemented for any kind of scientific or industrial application due to its cost-effective nature. The effect of sonication time on the nanoparticle formation was investigated. Silver nanoparticles were analyzed through transmission electron microscopy and UV-vis spectroscopy. Antimicrobial additives can be incorporated in mass in different matrixes (polymeric or cellulosic), which is a convenient methodology to achieve antimicrobial activity. In this work, silver nanoparticles were incorporated in cellulose using an ultrasonic bath technique. The most important aspect of cellulose containing silver nanoparticles prepared by this method is its high antimicrobial efficiency. The microbiological study was carried out by a standard agar technique. The analysis showed that cellulose with incorporated silver nanoparticles exhibited strong antimicrobial activity against

Sign in / Sign up

Export Citation Format

Share Document