scholarly journals The effect of Batak onion (Allium chinense) extract and powder adduction on the carcass characteristic of broiler chicken infected by Escherichia coli

2021 ◽  
Vol 782 (2) ◽  
pp. 022102
Author(s):  
A Sadeli ◽  
N D Hanafi ◽  
M Tafsin ◽  
Hamdan ◽  
K Syahputra
Keyword(s):  
Escherichia Coli ◽  
Broiler Chicken ◽  
Carcass Characteristic ◽  
Allium Chinense

scholarly journals Factors associated with Salmonella enterica and Escherichia coli during downtime in commercial broiler chicken barns in Ontario

2021 ◽  
Vol 100 (5) ◽  
pp. 101065
Author(s):  
Chelsea E. Course ◽  
Patrick Boerlin ◽  
Durda Slavic ◽  
Jean-Pierre Vaillancourt ◽  
Michele T. Guerin
Keyword(s):  
Escherichia Coli ◽  
Salmonella Enterica ◽  
Broiler Chicken ◽  
Factors Associated ◽  
Commercial Broiler

Effect of Prechill Fecal Contamination on Numbers of Bacteria Recovered from Broiler Chicken Carcasses Before and After Immersion Chilling

2004 ◽  
Vol 67 (9) ◽  
pp. 1829-1833 ◽  
Author(s):  
J. A. CASON ◽  
M. E. BERRANG ◽  
R. J. BUHR ◽  
N. A. COX
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Tap Water ◽  
Fecal Contamination ◽  
Bacterial Counts ◽  
E Coli ◽  
Before And After ◽  
Marked Area ◽  
Skin Samples

Paired carcass halves were used to test whether fecal contamination of skin during processing of broiler chickens can be detected by increased bacterial counts in samples taken before and after immersion chilling. In each of three trials, six freshly defeathered and eviscerated carcasses were cut in half, and a rectangle (3 by 5 cm) was marked with dots of ink on the breast skin of each half. One half of each pair was chosen randomly, and 0.1 g of freshly collected feces was spread over the rectangle with a spatula. After 10 min, both halves were sprayed with tap water for 10 to 15 s until feces could no longer be seen in the marked area. Both halves were sampled with a 1-min carcass rinse and were then put in a paddle chiller with other eviscerated carcasses for 45 min to simulate industrial immersion chilling. Immediately after chilling, each carcass half was subjected to another 1-min rinse, after which the skin within the rectangle was aseptically removed from the carcass halves and stomached. Rinses of fecally contaminated halves had significantly higher Enterobacteriaceae immediately before chilling, but there were no differences in coliform and Escherichia coli counts. After chilling, there were no differences in Enterobacteriaceae, coliform, and E. coli counts in rinse or skin samples from the paired carcass halves. Correlations were generally poor between counts in rinse and skin samples but were significant between prechill and postchill rinses for both control and fecally contaminated halves. Correlations were also significant between counts in rinses of control and contaminated halves of the same carcass after chilling. Bacterial counts in postchill carcass rinses did not indicate that fecal contamination occurred before chilling.

Microbiological Sampling of Poultry Carcass Portions by Excision, Rinsing, or Swabbing†

2005 ◽  
Vol 68 (12) ◽  
pp. 2718-2720 ◽  
Author(s):  
C. O. GILL ◽  
M. BADONI ◽  
L. F. MOZA ◽  
S. BARBUT ◽  
M. W. GRIFFITHS
Keyword(s):  
Escherichia Coli ◽  
Muscle Tissue ◽  
Broiler Chicken ◽  
Sampling Methods ◽  
The Other ◽  
Aerobic Bacteria ◽  
The Mean ◽  
Poultry Carcass

Groups of 25 skin-on thighs or skin-on, skinned, or tumbled breast portions of broiler chicken carcasses were sampled by excision of skin or muscle tissue, rinsing, or swabbing. Counts of total aerobic bacteria, coliforms, and Escherichia coli were recorded for each sample. For all types of carcass portions, the mean log counts and the total log counts obtained for each group of bacteria by excision or rinsing mostly differed by <0.5 log unit. However, the counts obtained by swabbing were generally >0.5 log unit lower than the smaller of the values obtained by the other two sampling methods.

Two IncHI2 Plasmid-Mediated Colistin-Resistant Escherichia coli Strains from the Broiler Chicken Supply Chain in Zhejiang Province, China

2020 ◽  
Vol 83 (8) ◽  
pp. 1402-1410
Author(s):  
JIANG CHANG ◽  
BIAO TANG ◽  
YIFEI CHEN ◽  
XIAODONG XIA ◽  
MINGRONG QIAN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Supply Chain ◽  
Broiler Chicken ◽  
Complete Sequence ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Complete Sequences ◽  
First Time ◽  
Genetic Context ◽  
Main Factor

ABSTRACT Colistin is used as one of the last-resort drugs against lethal infections caused by carbapenem-resistant pathogens of the Enterobacteriaceae family. Enterobacteriaceae bacteria carrying the mcr-1 colistin resistance gene are emerging in livestock and poultry, posing a serious threat to human health. However, there have been few reports about the prevalence and transmission of mcr-1 along the regional chicken supply chain. In this study, the complete sequences of mcr-1–positive Escherichia coli ST2705 and ST206 isolates obtained by screening 129 chilled chicken samples and 251 chicken fecal samples were investigated. Both of these isolates showed resistance to colistin, and importantly, the complete sequence of the mcr-1–positive E. coli ST2705 in China was reported for the first time. The mcr-1 gene was located on the IncHI2 plasmids pTBMCR421 (254,365 bp) and pTBMCR401 (230,964 bp) in strains ECCNB20-2 and ECZP248, respectively. Comparative analysis of mcr-1–bearing IncHI2 plasmids showed a marked similarity, indicating that these plasmids are very common and have the ability to be efficient vehicles for mcr-1 dissemination among humans, animals, and food. Furthermore, an insertion (ISKpn26) in Tn6330 (ISApl1-mcr-1-pap2-ISApl1) was identified in the plasmid pTBMCR401 and then compared; this insertion might affect the adaptability and stability of Tn6330. Taken together, these findings suggest that the IncHI2 plasmid might be a main factor affecting the transmission of mcr-1 in the chicken supply chain and that the genetic context of the mcr-1–bearing IncHI2 plasmid is constantly evolving. HIGHLIGHTS

Molecular and Phenotypic Characterization of Escherichia coli Isolated from Broiler Chicken Flocks in Egypt

2013 ◽  
Vol 57 (3) ◽  
pp. 602-611 ◽  
Author(s):  
Ashraf H. M. Hussein ◽  
Ibrahim A. I. Ghanem ◽  
Amal A. M. Eid ◽  
Mohamed A. Ali ◽  
Julie S. Sherwood ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chicken ◽  
Phenotypic Characterization

scholarly journals Antimicrobial Susceptibility of Escherichia coli Isolated from Iranian Broiler Chicken Flocks, 2005–2006

2008 ◽  
Vol 17 (2) ◽  
pp. 302-304 ◽  
Author(s):  
E. Saberfar ◽  
B. Pourakbari ◽  
K. Chabokdavan ◽  
F. Taj Dolatshahi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Broiler Chicken

scholarly journals Antimicrobial Resistance of Escherichia coli and Salmonella isolated from Raw Retail Broiler Chicken Carcasses in Zambia

2020 ◽  
Author(s):  
Elizabeth Muligisa Muonga ◽  
Geoffrey Mainda ◽  
Mercy Mukuma ◽  
Geoffrey Kwenda ◽  
Bernard Hang'ombe ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
Target Genes ◽  
Health Concern ◽  
Public Health Concern ◽  
E Coli ◽  
Genes Encoding

Abstract BackgroundAntimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries such as Zambia. This study was undertaken to determine the antimicrobial resistance profiles of Escherichia coli ( E. coli ) and Salmonella isolated from raw retail broiler chicken carcasses purchased from open and supermarkets in Zambia.ResultsA total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates were done using Analytical Profile Index (API 20E) (Biomerieux ® ) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 10 antibiotics. Multiplex PCR was used to determine the presence of three target genes encoding for resistance: tet A, Sul 1 and bla CTX-M . WHONET 2018 software was used to analyse AST results. The E. coli isolates were mostly resistant to tetracycline (79.4%), ampicillin (51.9%), and trimethoprim/sulfamethoxazole (49.7%). Two of the five Salmonella isolates were resistant to at least one antibiotic. Forty- seven (45.2%) of the 104 isolates that were screened for the presence of the resistant genes possessed at least one of the targeted resistance genes.ConclusionThis study has demonstrated the presence of AMR E. coli and Salmonella on raw retail broiler chicken carcasses from open and supermarkets, which is of public health concern.

Residu Antibiotik Serta Keberadaan Escherichia Coli Penghasil ESBL pada Daging Ayam Broiler di Pasar Kota Purwokerto

2021 ◽  
Vol 20 (2) ◽  
pp. 137-142
Author(s):  
Anriani Puspita Karunia Ning Widhi ◽  
Imam Nafi Yana Saputra
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Chicken Meat ◽  
Primary Data ◽  
Antibiotic Residues ◽  
Growth Promoters ◽  
E Coli ◽  
Extended Spectrum ◽  
Starting Point

Latar belakang: Pemenuhan kebutuhan pangan asal ternak yang berasal dari unggas memiliki angka konsumsi yang cukup tinggi salah satunya yaitu ayam broiler. Untuk memenuhi tingginya permintaan terhadap ayam broiler pada pakandiberifeed additiveserta antibiotic growth promotor (AGP) dalam bentuk antibiotik untuk mempercepat pertumbuhan dan daya tahan tubuh. Pemanfaatan antibiotik yang tidak bijak akan menimbulkan residu antibiotik serta resistensi Escherichia coli penghasil Extended Spectrum β-lactamase (ESBL).Metode: Penelitian ini termasuk penelitian deskriptif. Data primer diperoleh dari hasil screeningtest. Data ditunjukkan dalam tabel dangambar yang dibahas secara deskriptif. Variabel yang diamati residu antibiotik tetrasiklin serta E. coli penghasil ESBL pada daging ayam broiler.Hasil: Temuan terhadap residu antibiotik tetrasiklin pada daging ayam broiler yaitu sebesar 7,14% dengan rerata zona hambat 12,13 mm, sedangkan hasil identifikasi E. coli penghasil ESBL pada daging ayam broiler diperoleh hasil sebesar 71,4%.Simpulan:Residu antibiotik tetrasiklin dalam daging ayam broiler masih tergolong aman dapat dikonsumsi namun, temuanE. coli penghasil ESBL pada daging ayam broiler menimbulkan masalah kesehatan, baik bagi kesehatan hewan maupun manusia, serta dapat menimbulkan resistensi terhadap antibiotik. ABSTRACT Title: Broiler Chicken Meat Sold at Purwokerto's Market Has Antibiotic Residues and Escherichia Coli That Produces EsblBackground: Poultry, of which broiler chickens are one example, is a food source with a relatively high consumption rate.Feed additives and antibiotic growth promoters (AGP) in the form of antibiotics in the ration are given to increase broilers’ growth and endurance to meet the high demand for them. It is important to note that unwise antibiotic use results in the buildup of antibiotic residues and resistance to the Extended Spectrum Beta-lactamase(ESBL)produced by Escherichia coli bacterium.Method: To collect primary data for this descriptive study, the screening test results were employed as a starting point. Next, the information was presented in the form of tables and figures.Result: According to this study, broiler chickens had a tetracycline residue of 7.14 percent, with an inhibitory zone mean of 12.13 mm, and 71.4 percent of ESBL-producing E. coli.Conclusion:According to the study, broiler chicken meat with tetracycline residue is still safe to consume. E. coli, which is known to develop an antibiotic-resistant strain of E. coli ESBL) can cause serious health problems in both humans and animals.

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