scholarly journals The effectiveness of prostaglandin nanoparticles in corpus luteum regression

2021 ◽  
Vol 888 (1) ◽  
pp. 012005
Author(s):  
D A Kusumaningrum ◽  
R S Sianturi ◽  
F A Pamungkas ◽  
E Wina
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Corpus Luteum ◽  
Ionic Gelation ◽  
Estrus Synchronization ◽  
Progesterone Concentration ◽  
Hormone Profile ◽  
Hormone Administration ◽  
Follicle Size ◽  
Estrus Response

Abstract Research examined the formation of prostaglandin nanoparticles and their effect on corpus luteum (CL) regression carried out at IRIAP. The nanoparticles formation was carried out using the ionic gelation method. The nanoparticles have a particle size of 316.80±0.14 nm, polydispersion index of 0,453±0,001, zeta potential of +17,40±0,85 mV with 69,69±8.81% hormone entrapment. The effectiveness of nanoparticle in CL regression was observed (prostaglandin vs prostaglandin nanoparticles) using ultrasound observation, hormone profile and estrus response. Further, the size of the ovulating follicle, the time of ovulation, the size of the CL and the onset of estrus after the administration of the prostaglandins were observed. The observation showed that the intramuscular administration of prostaglandin and prostaglandin nanoparticles did not significantly differ on the onset of estrus, time of ovulation, the ovulating follicle size, size of CL and progesterone concentration. The onset of estrus occurred on 2.50 ± 0.58 and 2.33 ± 0.52 days, the ovulation time after hormone administration was on days 3.50 ± 0.55 and 2.83 ± 0.75 with the ovulation follicle size of 16, 62 ± 0.96 and 17.03 ± 1.13 mm, while the CL measures at H-3 were 13.56 ± 2.28 and 10.45 ± 0.88, the progesterone H-2 concentrations were 0.299 and 0.395, respectively for prostaglandin and prostaglandin nanoparticles. It can be concluded that the formation of nanoparticles did not impair the effectiveness of hormones in CL regression so that it can be used to increase the effectiveness of estrus synchronization

scholarly journals FORMULATION AND CHARACTERIZATION OF TIMOLOL MALEATE-LOADED NANOPARTICLES GEL BY IONIC GELATION METHOD USING CHITOSAN AND SODIUM ALGINATE

2019 ◽  
pp. 48-54 ◽  
Author(s):  
RISA AHDYANI ◽  
LARAS NOVITASARI ◽  
RONNY MARTIEN
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Sodium Alginate ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Entrapment Efficiency ◽  
Polydispersity Index ◽  
Ionic Gelation ◽  
Timolol Maleate ◽  
Optimum Formula

Objective: The objectives of this study were to formulate and characterize nanoparticles gel of timolol maleate (TM) by ionic gelation method using chitosan (CS) and sodium alginate (SA). Methods: Optimization was carried out by factorial design using Design Expert®10.0.1 software to obtain the concentration of CS, SA, and calcium chloride (CaCl2) to produce the optimum formula of TM nanoparticles. The optimum formula was characterized for particle size, polydispersity index, entrapment efficiency, Zeta potential, and molecular structure. Hydroxy Propyl Methyl Cellulose (HPMC) K15 was incorporated into optimum formula to form nanoparticles gel of TM and carried out in vivo release study using the Franz Diffusion Cell. Results: TM nanoparticles was successfully prepared with concentration of CS, SA, and CaCl2 of 0.01 % (w/v), 0.1 % (w/v), and 0.25 % (w/v), respectively. The particle size, polydispersity index, entrapment efficiency, and Zeta potential were found to be 200.47±4.20 nm, 0.27±0.0154, 35.23±4.55 %, and-5.68±1.80 mV, respectively. The result of FTIR spectra indicated TM-loaded in the nanoparticles system. In vitro release profile of TM-loaded nanoparticles gel showed controlled release and the Korsmeyer-Peppas model was found to be the best fit for drug release kinetics. Conclusion: TM-loaded CS/SA nanoparticles gel was successfully prepared and could be considered as a promising candidate for controlled TM delivery of infantile hemangioma treatment.

scholarly journals Physicochemical Properties andIn VitroCytotoxicity Studies of Chitosan as a Potential Carrier for Dicer-Substrate siRNA

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Maria Abdul Ghafoor Raja ◽  
Haliza Katas ◽  
Zariyantey Abd Hamid ◽  
Nur Atiqah Razali
Keyword(s):  
Particle Size ◽  
Cost Effectiveness ◽  
Zeta Potential ◽  
Small Interfering Rna ◽  
Ionic Gelation ◽  
Rapid Degradation ◽  
The Mean ◽  
Afm Micrographs ◽  
Interfering Rna

Recently, Dicer-substrate small interfering RNA (DsiRNA) has gained attention owing to its greater potency over small interfering RNA (siRNA). However, the use of DsiRNA is restricted by its rapid degradationin vitro. To address this issue, chitosan nanoparticulate deliver yplatform for the Dicer-substrate siRNA (DsiRNA) was developed and characterized. Nanoparticles were prepared by simple complexation and ionic gelation methods. The mean particle size of DsiRNA-adsorbed chitosan nanospheres (DsiRNA-CS NPs) prepared by the ionic gelation method ranged from 225 to 335 nm, while simple complexation yielded DsiRNA-chitosan complexes (DsiRNA-CS complexes) ranging from 270 to 730 nm. The zeta potential of both types of nanoparticles ranged from +40 to +65 mV. TEM and AFM micrographs revealed spherical and irregular morphology of DsiRNA-CS NPs and DsiRNA-CS complexes. ATR-FTIR spectroscopy confirmed the presence of DsiRNA in the CS NPs/complexes. Both types of nanoparticles exhibited sustained release and high binding and encapsulation (100%) efficiency of DsiRNA. DsiRNA-CS NPs/complexes showed low, concentration-dependent cytotoxicityin vitro. DsiRNA-CS NPs showed better stability than the complexes when stored at 4 and 25°C. Thus, it is anticipated that CS NPs are promising vectors for DsiRNA delivery due to their stability, safety, and cost-effectiveness.

scholarly journals Preparation and Characterization of Betel Leaves (Piper betle Linn) Extract Nanoparticle with Ionic Gelation Method

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Dwi Saryanti ◽  
Dian Nugraheni ◽  
Nisa Sindi Astuti
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Leaf Extract ◽  
Particle Morphology ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Piper Betle ◽  
Ionic Gelation ◽  
Betel Leaf

Nanoparticles are used in drug delivery which can increase mass transfer so increase the absorption and effectiveness of the drug. Therefore, its prospect to improve antibacterial and antioxidants activities of betel leaves. The research aimed to preparation and characterization of betel leaf extract using ionic gelation technique. The formulation of nanoparticles from betel leaf extract with ionic gelation method using alginate and CaCl2 with a ratio of 2.5: 1. The characterization of the nanoparticles includes particle size analysis, zeta potential, particle morphology and determination of flavonoid content. Particle size analysis demonstrated that the betel leaf extract nanoparticles had a particle size of 243,03 ± 1,48 nm, zeta potential of -23,0 ± 0,35 mV and morphology of particle showed that a flat shape. The betle leaf exctract nanoparticle positively contained flavonoid with Rf 0.7 equivalent to quercetin. The betel leaf extract can be made nanoparticles with ionic gelation method using alginate and CaCl2.

scholarly journals EKSTRAKSI DAN KARAKTERISASI NANOPARTIKEL EKSTRAK SIRIH MERAH (Piper crocat um) (Extraction and Characterization of Nanoparticles of Red Betel Leaves (Piper crocatum))

2017 ◽  
Vol 10 (2) ◽  
pp. 58 ◽  
Author(s):  
Kun Tanti Dewandari ◽  
Sri Yuliani ◽  
Sedarnawati Yasni
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Medicinal Plant ◽  
Organic Solvent ◽  
Antioxidant Capacity ◽  
Total Phenol ◽  
Ionic Gelation ◽  
English Version ◽  
Protein Isolates

<p>Sirih merah merupakan salah satu tanaman obat yang dapat dimanfaatkan sebagai minuman fungsional, karena mengandung senyawa fitokimia dari golongan alkaloid, flavonoid, dan tanin yang berkhasiat sebagai antihiperglikemik dan antioksidan. Salah satu kelemahan dalam penyerapan bahan aktif adalah bioavaibilitasnya yang rendah. Salah satu teknologi yang dapat digunakan adalah teknologi nano. Tujuan penelitian ini adalah melakukan ekstraksi sirih merah, sintesis nanopartikel dan karakterisasinya serta mengetahui stabilitasnya pada beberapa kondisi pH. Hasil penelitian menunjukkan bahwa ekstraksi etanol 96% dengan maserasi memberikan hasil yang terbaik dengan rendemen 7,2 ± 0,25%, kapasitas antioksidan 10892,86 ± 6,06 AAE?g/ml, IC 50 sebesar 46,51 ± 0,05 AAE?g/ml serta total fenol 2388,37 ± 0,3 mg/100g dengan komponen volatil utama yaitu sabinen dan mirsen. Konsentrasi kitosan 0,2% dengan rata-rata diameter 197,20 ± 11,68 nm memberikan hasil yang terbaik dengan nilai IP 0,235 ± 0,03, zeta potensial 32,75 ± 2,11 mV, kapasitas antioksidan 5502,00 ± 8,48 AAE?g/ml, nilai IC 50 yaitu 279,10 ± 0,05 AAE?g/ml dan total fenol 568,76 ± 3,0 mg/100g. Enkapsulasi nanopartikel dengan penyalut campuran maltodekstrin dan isolat protein menunjukkan terjadi peningkatan ukuran partikel dimana dengan pengisi maltodekstrin (M) sebesar 8952,7 ± 2598 nm dan campuran maltodekstrin dan isolat protein kedelai sebesar 8266,9 ± 1134,9 nm. Stabilitas pada beberapa kondisi pH menunjukkan bahwa penurunan persentase total fenol terbesar pada pH basa (6,7, dan 8) dibandingkan pada kondisi pH asam (2,3 dan 4).</p><p>Kata kunci :ekstraksi, antioksidan, daun sirih merah, nanopartikel</p><p>English Version Abstract</p><p>Red betel, a medicinal plant containing alcaloids, flavonoids and tannins, has health benneficial effects as antihyperglycemics and antioxidants. However, its low bioavailablity limit the applications of this extract for nutraceuticals. Transformation of extract into nanoparticles through ionic gelation process was done to enhance its bioavailability. This study is aimed at extracting the active ingredients of red betel leaves using organic solvent, preparing nanoparticles, and characterizing their properties including their stability at different pHs. The study showed the highest yield of red betel leaves extract was observed in the extraction using ethanol 96% (7.2 ± 0,25%) with the capacity of antioxidant of 10892.86 ± 6.06 AAE?g/ml, the IC 50 of 46.51 ± 0.05 AAE?g/ml, the total phenol of 2388.37 ± 0.3 mg/100g and the major volatile compounds of sabinene dan myrcene. Chitosan at a concentration of 0.2% produced nanoparticle size of 197.20 ± 11.68 nm with PDI 0,235 ± 0,03, zeta potential 32.75 ± 2.11mV, antioxidant capacity 5502.00 ± 8.48 AAE?g/ml, nilai IC 50 yaitu 279,10 ± 0,05 AAE?g/ml dan total fenol 568,76 ± 3,0 mg/100g. Encapsulation of nanoparticles using maltodextrin and protein isolates resulted in increases in particle size, in which maltodextrin gave slightly largle particles (8952.7 ± 2598 nm) than did combination of maltodextrin and soy proten isolate (8266.9 ± 1134.9 nm). Nanoparticles at pHs of 6, 7 and 8 exhibited larger decreases in total phenol as compared to that at lower pHs (2, 3, and 4).</p><p>Keywords : extraction, antioxidant, red betel leaves, nanoparticles</p>

scholarly journals OPTIMIZATION OF STIRRING SPEED AND STIRRING TIME TOWARD NANOPARTICLE SIZE OF CHITOSAN-SIAM CITRUS PEEL (Citrus nobilis L.var Microcarpa) 70% ETHANOL EXTRACT

2017 ◽  
Vol 22 (1) ◽  
pp. 16
Author(s):  
Wintari Taurina ◽  
Rafika Sari ◽  
Uray Cindy Hafinur ◽  
Sri Wahdaningsih ◽  
Isnindar Isnindar
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Small Particle ◽  
Ethanol Extract ◽  
Entrapment Efficiency ◽  
Ionic Gelation ◽  
Small Particle Size ◽  
Citrus Peel ◽  
West Kalimantan ◽  
Stirring Time

Siam citrus peel (Citrus nobilis L. var. Microcarpa) is a plant derived from Sambas Regency, West Kalimantan Province. Bioavailability of herbal active compounds can be enhanced by formulating extract into nanoparticle. The polymer used was chitosan with crosslinker Na-TPP. Stirring speed and stirring time play an important role to produce small particle size in forming nanoparticle using ionic gelation method. Enhancement of stirring speed and stirring time could reduce particle size. Nanoparticles were prepared using ionic gelation method by mixing Na-TPP, extract and chitosan (1:1:6) with varying the stirring speed 500 rpm, 1000 rpm, 1500 rpm and stirring time 1 hrs, 2 hrs, 3 hrs. The particle size of nanoparticle was found to be 85.3 nm at 1000 rpm of stirring speed and 3 hrs of stirring times, with polidispersity index 0.287, zeta potential +32.37 mV and entrapment efficiency 87.12 %.

scholarly journals Follicle and corpus luteum size and vascularity as predictors of fertility at the time of artificial insemination and embryo transfer in beef cattle

2015 ◽  
Vol 35 (5) ◽  
pp. 470-476 ◽  
Author(s):  
Fábio L.V. Pinaffi ◽  
Ériko S. Santos ◽  
Maurício G. da Silva ◽  
Milton Maturana Filho ◽  
Ed H. Madureira ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Corpus Luteum ◽  
Embryo Transfer ◽  
Artificial Insemination ◽  
Prediction Methods ◽  
Estrous Synchronization ◽  
Hormone Profile ◽  
Lower Fertility ◽  
Follicle Size ◽  
First Time

Abstract:Two ultrasound based fertility prediction methods were tested prior to embryo transfer (ET) and artificial insemination (AI) in cattle. Female bovines were submitted to estrous synchronization prior to ET and AI. Animals were scanned immediately before ET and AI procedure to target follicle and corpus luteum (CL) size and vascularity. In addition, inseminated animals were also scanned eleven days after insemination to target CL size and vascularity. All data was compared with fertility by using gestational diagnosis 35 days after ovulation. Prior to ET, CL vascularity showed a positive correlation with fertility, and no pregnancy occurred in animals with less than 40% of CL vascularity. Prior to AI and also eleven days after AI, no relationship with fertility was seen in all parameters analyzed (follicle and CL size and vascularity), and contrary, cows with CL vascularity greater than 70% exhibit lower fertility. In inseminated animals, follicle size and vascularity was positive related with CL size and vascularity, as shown by the presence of greater CL size and vascularity originated from follicle with also greater size and vascularity. This is the first time that ultrasound based fertility prediction methods were tested prior to ET and AI and showed an application in ET, but not in AI programs. Further studies are needed including hormone profile evaluation to improve conclusion.

scholarly journals Early Pregnancy in Jennies in the Caribbean: Corpus Luteum Development and Progesterone Production, Uterine and Embryo Dynamics, Conceptus Growth and Maturation

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 127
Author(s):  
Lorenzo G. T. M. Segabinazzi ◽  
Brandy N. Roberts ◽  
Erik W. Peterson ◽  
Rachael Ambrosia ◽  
Don Bergfelt ◽  
...  
Keyword(s):  
Linear Regression ◽  
Corpus Luteum ◽  
Random Effects ◽  
Early Pregnancy ◽  
Early Embryo ◽  
Progesterone Concentration ◽  
Early Embryo Development ◽  
The Third ◽  
Hormone Profile ◽  
The Caribbean

We aimed to characterize early embryo development and changes in corpus luteum (CL) development and progesterone profile in pregnant vs. non-pregnant jennies. Eight jennies were enrolled in the study. In the first two cycles, the jennies were monitored by transrectal ultrasonography and had blood harvested for hormone profile assay. In the third cycle, jennies were bred by a jack of proven fertility. Jennies were then monitored and sampled for up to 30 days of pregnancy. Data were evaluated by random-effects multiple linear regression, and correlations were expressed as Pearson’s correlation coefficient. Progesterone concentration rose rapidly from ovulation (D0) until D7, plateaued until D12–14, then precipitously declined between D14 and 15, remaining low until the next ovulation in non-pregnant cycles. In the pregnant jennies, the progesterone concentration rose to maximal concentrations on D7–11, being higher at this stage than in non-pregnant cycles, then declined gradually up to D30. In all cycles, the volume of the CL increased steadily until D6, when it plateaued in pregnant jennies. For non-pregnant jennies, CL volume decreased slowly from D6 to D11 and then had a faster drop. Uterine tone increased following ovulation, becoming turgid around the day of embryo fixation (D15.0 ± 0.9). An embryonic vesicle (EV) was first detected on D9.3 ± 0.5 (2.4 ± 0.5 mm). The EV remained spherical until D18.6 ± 1.4. The embryo proper was first detected ventrally in the vesicle on D20.8 ± 1.1 and the embryonic heartbeat by D22.0 ± 0.9. The allantoic sac was identified at D24.0 ± 0.9, and at D30, the allantoic sac filled the ventral half of the EV. This study provides evidence that higher cumulative concentrations of progesterone are correlated to size of the EV, and there were changes in the luteal dynamics and progesterone profiles in pregnant vs. non-pregnant jennies.

scholarly journals Nanoparticle Formula Optimization of Juwet Seeds Extract (Syzygium cumini) using Simplex Lattice Design Method

2020 ◽  
Vol 20 (3) ◽  
pp. 416
Author(s):  
Yohanes Juliantoni ◽  
Wahida Hajrin ◽  
Windah Anugrah Subaidah
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Optimization Design ◽  
Entrapment Efficiency ◽  
Seed Extract ◽  
Ionic Gelation ◽  
Syzygium Cumini ◽  
Lattice Design ◽  
Simplex Lattice Design ◽  
Simplex Lattice

Diabetes is a degenerative disease whose numbers continue to increase from year to year. Treatment with oral antidiabetic drugs can cause harmful side effects for patients. One of the plants that can be used for diabetes therapy is juwet (Syzygium cumini L.). Flavonoid compounds of juwet seed is easily oxidized and hydrolyzed in intestinal fluids with low absorption. This problem can be overcome by forming nanoparticle of juwet seed extract. The aims of this study was to obtain the formula for juwet seed nanoparticles. Nanoparticle of juwet seed extract made by ionic gelation method with chitosan and Na-TPP polymers which are varied. The formula optimization design in this study was determined using a simplex lattice design by 3 variations of materials based on the parameters %entrapment efficiency, %transmittant, particle size, polydisperse index, and zeta potential. The results showed that the yield of duwet seed extract was 7,44%. Juwet seed ethanolic extract contains 3.33 mg of quercetin per 1 g of extract. Based on the desirability value approach, the optimum formula was produced with an extract composition of 1.650 mL; chitosan 0.500 mL; and Na-TPP 0.010 mL can give optimal results with a desirability value of 0.772 which results in the criteria of 63.66% entrapment efficiency; 50.56% transmittant; 615,833nm particle size; 0.620 PDI; and zeta potential of +13.89 mV. Juwet seeds extract can be formulated into the form of nanoparticles. To get better parameters, it is advisable to optimize the mixing time and mixing speed in the ionic gelation method.

scholarly journals Nanoparticle preparation and characterization of Haruan fish (Channa striata) exctract contains albumin from south Kalimantan with ionic gelation method

2017 ◽  
Vol 9 (2) ◽  
pp. 47
Author(s):  
Rahmawanty D ◽  
Risa A ◽  
Malikhatun N ◽  
Prima HR ◽  
Nani K ◽  
...  
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Entrapment Efficiency ◽  
Ionic Gelation ◽  
Transmission Electron ◽  
Particle Size Distribution Measurement ◽  
Particle Size Analyzer ◽  
Nanoparticle Preparation ◽  
Channa Striata

<p>Snakehead fish (<em>Channa striata</em>) has been reported to be used for wound healing by people in South Borneo because it contains albumin. Snakehead fish extract (<em>Channa striata</em>) has hydrophillic property and poor stability. Nanoparticle technology has been started to be developed as an alternative solution to improve drug delivery profile. The purpose of this study was to determine the formulation that obtained best characterization for nanoparticle. Nanoparticles were prepared by ionic gelation method, that was prepared by doing optimize ratio between snakehead fish extract : chitosan and pH of chitosan solvent.Nanoparticles were characterized using Particle Size Analyzer for particle size and particle size distribution, measurement of entrapment efficiency, determined Zeta potential using Particle Size Analyzer, and observation of particle’s morphology using Transmission Electron Microscope. The result showed that the chosen formula was formula 6 which  ratio of extract : chitosan 1:2 with chitosan solvent pH 3, particle size 152.3 nm, polidispersity index 0.778, percentage of entrapment efficiency 51.3961 %, Zeta potential +35.9 mV, and round shape of particles.</p>

scholarly journals ANTIDIABETIC ACTIVITY OF NANOPARTICLES CONTAINING JAVANESE TURMERIC RHIZOME EXTRACT: THE STRATEGY TO CHANGE PARTICLE SIZE

2020 ◽  
pp. 90-93
Author(s):  
DENI RAHMAT ◽  
YUNAHARA FARIDA ◽  
AVILLA TAN BRYLIANTO ◽  
ROS SUMARNY ◽  
SHIRLY KUMALA
Keyword(s):  
Particle Size ◽  
Blood Glucose ◽  
Zeta Potential ◽  
Solvent System ◽  
Antidiabetic Activity ◽  
Ionic Gelation ◽  
Diabetic Mice ◽  
Drying Process ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective: The aim of this study was to investigate the antidiabetic activity of Javanese turmeric rhizome extract and its nanoparticles. Javanese turmeric rhizome contains curcumin, flavonoid, and xanthorrhizol that show the antidiabetic activity. The plant extracts entrapped within the nanoparticles have been reported to increase the active compound bioavailability. Thereby, the nanoparticles can improve the biological activity of active compounds. Methods: The rhizome was extracted by kinetic maceration using 96% (v/v) ethanol, whereas the nanoparticles were prepared by the ionic gelation method with different formulations to obtain two types of nanoparticles (F1 and F2). The resulting nanoparticles were evaluated for their particle size, zeta potential, and morphology. The antidiabetic study was performed in a model of alloxan-induced diabetic mice. The mice were divided into five groups, namely normal, negative, positive, extract (dose of 400 mg/kg) and nanoparticles group (dose of 400 mg/kg). Afterward, the blood glucose levels were observed within 24 d using a glucometer. Results: The nanoparticles F1 displayed particle size of 157.8±18.0 nm with a polydispersity index of 0.488 and zeta potential of+51.4±4.56 mV. Spray drying process of the suspension of nanoparticles F1 produced a fine yellow powder. In contrast, nanoparticles F2 assembled with a different solvent system gave rise to a smaller particles size (90±20.8 nm) but could not be dried. Hence, the nanoparticles F1 were further studied for the antidiabetic study. The results showed that the nanoparticles; F1 rendered a better antidiabetic activity compared to the extracts. Percentage of decrease in blood glucose levels of the extract and the nanoparticles were 39.62 and 47.52%, respectively. Conclusion: The type of solvent system of the extract could interfere with the resulting particle size of the nanoparticles. The nanoparticles could be promising carriers for the extract to improve the antidiabetic activity.

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