In vitro gas production and cinetical fermentation of a few local feed in Kupang East Nusa Tenggara

Abstract Research objectives were to evaluate and to analyze in vitro gas production and cinetical fermentation of Kupang’s local feed. The feed materials were dried and grinded for nutrient and gas produced analysis. Collected data were chemical composition, in vitro gas production, cinetical fermentation, short chain fatty acids, energy metabolic, and organic matter digestation. The result showed that the materials have different gas production. Commonly, the higher the carbohydrate content the higher the gas production; the higher the protein and the fat content the lower the gas production. Total gas productions of each material after 96 hours incubation were 53.88 ml/0.5 g DM for the high fat content feed, 92.26 and 159.25 ml/0.5 DM for the high protein and high carbohydrate content. Regarding total production, the fermented fraction gas productions were 44.662; 82.54 and 153.462 ml/0.5 g DM. The short chain fatty acids produced were 0.31; 0.51 and 1.28 mM. The feed organic matter digestations produced were 42.2; 55.91 and 68.8 % for each feed with a high content of fat, protein, and carbohydrate. Conclusively comparing with the protein and carbohydrate feed sources, the higher the fat content the lower the gas production, short chain fatty acids, and organic matter digestion.

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In vitro Gas Production and its Prediction on Metabolizable Energy, Organic Matter Digestibility and Short Chain Fatty Acids of Some Tropical Seeds

Pakistan Journal of Nutrition ◽

10.3923/pjn.2009.1078.1082 ◽

2009 ◽

Vol 8 (7) ◽

pp. 1078-1082 ◽

Cited By ~ 1

Author(s):

O.J. Babayemi ◽

M.A. Bamikole ◽

Modupe O. Daodu

Keyword(s):

Fatty Acids ◽

Organic Matter ◽

Short Chain Fatty Acids ◽

Gas Production ◽

Metabolizable Energy ◽

Short Chain ◽

In Vitro Gas Production ◽

Organic Matter Digestibility ◽

Tropical Seeds

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Greenhouse gases, short-chain fatty acids and ruminal pH in vitro of biodiesel byproducts to replace corn silage

Revista Brasileira de Saúde e Produção Animal ◽

10.1590/s1519-99402015000400017 ◽

2015 ◽

Vol 16 (4) ◽

pp. 935-947 ◽

Cited By ~ 5

Author(s):

Fabíola Franklin de MEDEIROS ◽

Leilson Rocha BEZERRA ◽

Aderbal Marcos de Azevêdo SILVA ◽

Heloisa CARNEIRO ◽

Raissa Kiara Oliveira de MORAIS ◽

...

Keyword(s):

Fatty Acids ◽

Ricinus Communis ◽

Short Chain Fatty Acids ◽

Rumen Fermentation ◽

Gas Production ◽

Corn Silage ◽

Short Chain ◽

Acetate Butyrate ◽

Chain Fatty Acids

SUMMARY The aim of the study was evaluate the production potential for methane (CH4) and carbon dioxide (CO2), short-chain fatty acids, ammonia nitrogen (N-NH3) and pH by semi-automated techniquein vitro from biodiesel byproducts cottonseed cake (Gossypium hirsutum), castor bean (Ricinus communis), moringa cake (Moringa oleifera), jatropha cake (Jatropha curcas) and sunflower cake (Helianthus annuus) substituting corn silage in increasing levels, 0, 30, 50 and 70%. The experimental design used was completely randomized in a 5 x 4 factorial arrangement (byproducts and substitution levels). The inoculum for the in vitro incubations was obtained from three Holstein cows with rumen fistulas. In the experiment, the conditions were verified for the differences in potential gas production among the ingredients. The byproduct of cotton was the ingredient with the greatest potential to produce acetate, butyrate, CO2 and CH4. The byproduct of moringa had the lowest potential for the production of acetate, butyrate, CO2 and CH4 from in vitro degraded dry matter and a greater potential for the production of propionate. Among the byproducts studied, moringa was distinguished for promoting mitigation of CH4 and obtaining levels of pH and N-NH3 satisfactory for maximum rumen fermentation; thus, it is recommended the byproduct of moringa to replace corn silage because reduces environmental impact without impairingin vitro rumen fermentation.

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Influence of diet on growth yields of rumen micro-organisms in vitro andin vivo: influence on growth yield of variable carbon fluxes to fermentation products

British Journal Of Nutrition ◽

10.1079/bjn2003934 ◽

2003 ◽

Vol 90 (3) ◽

pp. 625-634 ◽

Cited By ~ 41

Author(s):

M. Blümmel ◽

A. Karsli ◽

J. R. Russell

Keyword(s):

Fatty Acids ◽

Microbial Biomass ◽

Short Chain Fatty Acids ◽

Gas Production ◽

Microbial Production ◽

Short Chain ◽

Maize Grain ◽

Chain Fatty Acids

The efficiency of rumen microbial production (EMP)in vitroandin vivowas examined for three roughages (lucerne (Medicago sativaL.) hay, oat (Avenia sativaL.)–berseem clover (Trifolium alexandrinumcultivar BigBee) hay and maize (Zea maysL.) crop residue (MCR)) and for five isonitrogenous (106 g crude protein (N × 6·25)/kg) diets formulated from lucerne hay, oat–berseem clover hay, MCR, soya-bean meal and maize grain to provide degradable intake protein for the production of 130 g microbial protein/kg total digestible nutrients. EMPin vivowas determined by intestinal purine recovery in sheep and ranged from 240 to 360 g microbial biomass/kg organic matter truly degraded in MCR and in one of the diets respectively (P<0·05). EMPin vitrowas estimated by the substrate degraded: gas volume produced thereby (termed partitioning factor, PF (mg/ml)) at times of estimated peak microbial production and after 16·0 and 24·0h of incubation. For the diets, PF values were significantly related to EMPin vivoat peak microbial production (P= 0·04), but not after 16·0 (P= 0·08) and 24·0h (P= 0·66). For roughages, PF values were significantly related to EMPin vivoonly when measured after 16·0 h (P= 0·04). For MCR and diets, a close non-linear relationship was found between PF values at peak microbial production and EMPin vivo(R20·99,P<0·0001) suggesting a maximum EMPin vivoof 0·39. Low gas production per unit substrate degraded (high PF) was associated with high EMPin vivo.Thein vitrostudy of the products of fermentation, short-chain fatty acids, gases and microbial biomass (by purine analysis) after 16·0h of incubation showed very strong relationships (R2≥ 0·89,P<0·0001) between short-chain fatty acids, gases and gravimetrically measured apparent degradability. Except for maize grain, the true degradability of organic matter estimated by neutral-detergent solution treatment agreed with the sum of the products of fermentation (R20·81,P=0·0004). After 16·0h of incubation, the synergistic effects of diet ingredient on diets were greater for microbial biomass (18%) than for short-chain fatty acids and gas production (7 %). It is concluded that measurement of gas production only gives incomplete information about fodder quality; complementation of gas measurements by true degradability measurements is recommended.

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Relationship between the production of short chain fatty acids and gas when proteins are incubated in vitro

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200005160 ◽

2001 ◽

Vol 2001 ◽

pp. 134-134 ◽

Cited By ~ 2

Author(s):

C. Rymer ◽

S. Fakhri ◽

A.R. Moss ◽

D.I. Givens

Keyword(s):

Fatty Acids ◽

Short Chain Fatty Acids ◽

Gas Production ◽

Short Chain ◽

Good Relationship ◽

Wide Range ◽

Protein Rich ◽

The Relationship ◽

Chain Fatty Acids

The in vitro gas production technique is a means of measuring the dynamics of fermentation. If Wolin (1960) stoichiometry is assumed, and the amount of short chain fatty acids (SCFA) produced during an incubation are measured, the volume of gas produced can be predicted (Blümmel et al., 1997). When carbohydrate rich feeds are incubated, observed and predicted gas volumes are well related (Getachew et al., 1998). Blümmel et al. (1999) also observed a good relationship between observed and predicted gas volumes across a wide range of feeds. However, it was suggested by Cone and van Gelder (1999) that when proteins were incubated in vitro, the relationship was poor, which would suggest that the fermentation of proteins in vitro does not follow Wolin (1960) stoichiometry. The objective of this study was to investigate the relationship between observed and predicted gas volumes when protein rich feeds were incubated in vitro.

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The amount of rumen fluid influences the composition of short chain fatty acids produced in vitro

BSAP Occasional Publication ◽

10.1017/s146398150004231x ◽

2006 ◽

Vol 34 ◽

pp. 87-92

Author(s):

S. Muetzel ◽

R.M. Mauricio ◽

K. Becker

Keyword(s):

Fatty Acids ◽

Rumen Fluid ◽

Short Chain Fatty Acids ◽

Gas Production ◽

Short Chain ◽

Microbial Composition ◽

Fatty Acid Production ◽

Fermentation Products ◽

Chain Fatty Acids

SummaryIn vitro rumen incubation systems are widely used to determine substrate degradation and the amount of fermentation products like gases, short chain fatty acids and the microbial biomass produced. Here we compare the influence of the amount of inoculum used for preparing the incubation medium on short chain fatty acid production and composition. Treatments were 10% and 30% (v/v) of filtered rumen fluid used for inoculation. In a series of experiments it was demonstrated that the two treatments did not alter the gas production or the amount of substrate truly degraded. However, SCFA production (especially acetate) was significantly reduced when only 10% of inoculum were used for incubation. We propose that acetate was taken up by rumen microorganisms as a precursor for growth, although it cannot be excluded that an altered microbial composition contributed to the observed differences.

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