scholarly journals Influence of diet on growth yields of rumen micro-organisms in vitro andin vivo: influence on growth yield of variable carbon fluxes to fermentation products

2003 ◽  
Vol 90 (3) ◽  
pp. 625-634 ◽  
Author(s):  
M. Blümmel ◽  
A. Karsli ◽  
J. R. Russell
Keyword(s):  
Fatty Acids ◽  
Microbial Biomass ◽  
Short Chain Fatty Acids ◽  
Gas Production ◽  
Microbial Production ◽  
Short Chain ◽  
Maize Grain ◽  
Chain Fatty Acids

The efficiency of rumen microbial production (EMP)in vitroandin vivowas examined for three roughages (lucerne (Medicago sativaL.) hay, oat (Avenia sativaL.)–berseem clover (Trifolium alexandrinumcultivar BigBee) hay and maize (Zea maysL.) crop residue (MCR)) and for five isonitrogenous (106 g crude protein (N × 6·25)/kg) diets formulated from lucerne hay, oat–berseem clover hay, MCR, soya-bean meal and maize grain to provide degradable intake protein for the production of 130 g microbial protein/kg total digestible nutrients. EMPin vivowas determined by intestinal purine recovery in sheep and ranged from 240 to 360 g microbial biomass/kg organic matter truly degraded in MCR and in one of the diets respectively (P<0·05). EMPin vitrowas estimated by the substrate degraded: gas volume produced thereby (termed partitioning factor, PF (mg/ml)) at times of estimated peak microbial production and after 16·0 and 24·0h of incubation. For the diets, PF values were significantly related to EMPin vivoat peak microbial production (P= 0·04), but not after 16·0 (P= 0·08) and 24·0h (P= 0·66). For roughages, PF values were significantly related to EMPin vivoonly when measured after 16·0 h (P= 0·04). For MCR and diets, a close non-linear relationship was found between PF values at peak microbial production and EMPin vivo(R20·99,P<0·0001) suggesting a maximum EMPin vivoof 0·39. Low gas production per unit substrate degraded (high PF) was associated with high EMPin vivo.Thein vitrostudy of the products of fermentation, short-chain fatty acids, gases and microbial biomass (by purine analysis) after 16·0h of incubation showed very strong relationships (R2≥ 0·89,P<0·0001) between short-chain fatty acids, gases and gravimetrically measured apparent degradability. Except for maize grain, the true degradability of organic matter estimated by neutral-detergent solution treatment agreed with the sum of the products of fermentation (R20·81,P=0·0004). After 16·0h of incubation, the synergistic effects of diet ingredient on diets were greater for microbial biomass (18%) than for short-chain fatty acids and gas production (7 %). It is concluded that measurement of gas production only gives incomplete information about fodder quality; complementation of gas measurements by true degradability measurements is recommended.

Short-Chain Fatty Acids Prevent Diabetic Nephropathy In Vivo and In Vitro

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 92-OR ◽  
Author(s):  
WEI HUANG ◽  
YONG XU ◽  
YOUHUA XU ◽  
LUPING ZHOU ◽  
CHENLIN GAO
Keyword(s):  
Fatty Acids ◽  
Diabetic Nephropathy ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Chain Fatty Acids

scholarly journals Greenhouse gases, short-chain fatty acids and ruminal pH in vitro of biodiesel byproducts to replace corn silage

2015 ◽  
Vol 16 (4) ◽  
pp. 935-947 ◽  
Author(s):  
Fabíola Franklin de MEDEIROS ◽  
Leilson Rocha BEZERRA ◽  
Aderbal Marcos de Azevêdo SILVA ◽  
Heloisa CARNEIRO ◽  
Raissa Kiara Oliveira de MORAIS ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ricinus Communis ◽  
Short Chain Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Corn Silage ◽  
Short Chain ◽  
Acetate Butyrate ◽  
Chain Fatty Acids

SUMMARY The aim of the study was evaluate the production potential for methane (CH4) and carbon dioxide (CO2), short-chain fatty acids, ammonia nitrogen (N-NH3) and pH by semi-automated techniquein vitro from biodiesel byproducts cottonseed cake (Gossypium hirsutum), castor bean (Ricinus communis), moringa cake (Moringa oleifera), jatropha cake (Jatropha curcas) and sunflower cake (Helianthus annuus) substituting corn silage in increasing levels, 0, 30, 50 and 70%. The experimental design used was completely randomized in a 5 x 4 factorial arrangement (byproducts and substitution levels). The inoculum for the in vitro incubations was obtained from three Holstein cows with rumen fistulas. In the experiment, the conditions were verified for the differences in potential gas production among the ingredients. The byproduct of cotton was the ingredient with the greatest potential to produce acetate, butyrate, CO2 and CH4. The byproduct of moringa had the lowest potential for the production of acetate, butyrate, CO2 and CH4 from in vitro degraded dry matter and a greater potential for the production of propionate. Among the byproducts studied, moringa was distinguished for promoting mitigation of CH4 and obtaining levels of pH and N-NH3 satisfactory for maximum rumen fermentation; thus, it is recommended the byproduct of moringa to replace corn silage because reduces environmental impact without impairingin vitro rumen fermentation.

The Athlete and Gut Microbiome: Short-chain Fatty Acids as Potential Ergogenic Aids for Exercise and Training

2021 ◽  
Author(s):  
Tindaro Bongiovanni ◽  
Marilyn Ong Li Yin ◽  
Liam Heaney
Keyword(s):  
Fatty Acids ◽  
Gut Microbiome ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Future Research ◽  
Immune Functions ◽  
Additional Energy ◽  
Chain Fatty Acids

AbstractShort-chain fatty acids (SCFAs) are metabolites produced in the gut via microbial fermentation of dietary fibers referred to as microbiota-accessible carbohydrates (MACs). Acetate, propionate, and butyrate have been observed to regulate host dietary nutrient metabolism, energy balance, and local and systemic immune functions. In vitro and in vivo experiments have shown links between the presence of bacteria-derived SCFAs and host health through the blunting of inflammatory processes, as well as purported protection from the development of illness associated with respiratory infections. This bank of evidence suggests that SCFAs could be beneficial to enhance the athlete’s immunity, as well as act to improve exercise recovery via anti-inflammatory activity and to provide additional energy substrates for exercise performance. However, the mechanistic basis and applied evidence for these relationships in humans have yet to be fully established. In this narrative review, we explore the existing knowledge of SCFA synthesis and the functional importance of the gut microbiome composition to induce SCFA production. Further, changes in gut microbiota associated with exercise and various dietary MACs are described. Finally, we provide suggestions for future research and practical applications, including how these metabolites could be manipulated through dietary fiber intake to optimize immunity and energy metabolism.

scholarly journals Studies on lipid digestion in the preruminant calf

1978 ◽  
Vol 40 (1) ◽  
pp. 125-131 ◽  
Author(s):  
J. D. Edwards-Webb ◽  
S. Y. Thompson
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Butyric Acid ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Esterified Fatty Acids ◽  
Chain Fatty Acids

1. The lipolysis of cow's milk fat by salivary lipase (EC 3.1.1.3) in the preruminant calf has been studied in vitro by a simulated abomasal digestion, and also in vivo by examining the abomasal effluent collected over 12 h after giving whole milk to a calf.2. In the in vitro experiment the liquid drained from the clot contained a higher proportion of short-chain fatty acids than the abomasal effluent in the in vivo experiment. This was considered to indicate the absorption of short-chain free fatty acids from within the abomasum.3. Preferential release of short-chain fatty acids both in vitro and in vivo was observed.4. The outflow of butyric acid from the abomasum of the calf was initially rapid, but had levelled off at approximately 6 h, whereas the outflow of a typical long-chain fatty acid (palmitic) was fairly constant over the 12 h.Butyric acid predominated in the free fatty acids of abomasal effluent 0.5 h after feeding (668 mmol/mol total free fatty acids) but had become a minor component by 12 h (15 mmol/mol total free fatty acids).5. The mean amounts of free and esterified fatty acids (mmol/mol fatty acid ingested) present in the abomasal effluent from the 12 h collection period were: triglyceride 465, diglyceride 215, monoglyceride 68, free fatty acid 252. These values showed that only one-third of esterified fatty acids ingested are lipolysed to absorbable products by salivary lipase.

scholarly journals Production, Structural Characterization, and In Vitro Assessment of the Prebiotic Potential of Butyl-Fructooligosaccharides

2020 ◽  
Vol 21 (2) ◽  
pp. 445 ◽  
Author(s):  
Sini Kang ◽  
Hyun Ju You ◽  
Yeong-Geun Lee ◽  
Yunju Jeong ◽  
Tony V. Johnston ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Negative Control ◽  
Short Chain ◽  
Control Group ◽  
Metabolic Genes ◽  
Chain Fatty Acids

Short-chain fatty acids (SCFAs), especially butyrate, produced in mammalian intestinal tracts via fermentation of dietary fiber, are known biofunctional compounds in humans. However, the variability of fermentable fiber consumed on a daily basis and the diversity of gut microbiota within individuals often limits the production of short-chain fatty acids in the human gut. In this study, we attempted to enhance the butyrate levels in human fecal samples by utilizing butyl-fructooligosaccharides (B-FOS) as a novel prebiotic substance. Two major types of B-FOS (GF3-1B and GF3-2B), composed of short-chain fructooligosaccharides (FOS) bound to one or two butyric groups by ester bonds, were synthesized. Qualitative analysis of these B-FOS using Fourier transform infrared (FT-IR) spectroscopy, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), nuclear magnetic resonance (NMR) and low-resolution fast-atom bombardment mass spectra (LR-FAB-MS), showed that the chemical structure of GF3-1B and GF3-2B were [O-(1-buty-β-D-fru-(2→1)-O-β-D-fru-(2→1)-O-β-D-fru-O-α-D-glu] and [O-(1-buty)-β-D-fru-(2→1)-O-β-D-fru-(2→1)-O-(4-buty)-β-D-fru-O-α-D-glu], respectively. The ratio of these two compounds was approximately 5:3. To verify their biofunctionality as prebiotic oligosaccharides, proliferation and survival patterns of human fecal microbiota were examined in vitro via 16S rRNA metagenomics analysis compared to a positive FOS control and a negative control without a carbon source. B-FOS treatment showed different enrichment patterns on the fecal microbiota community during fermentation, and especially stimulated the growth of major butyrate producing bacterial consortia and modulated specific butyrate producing pathways with significantly enhanced butyrate levels. Furthermore, the relative abundance of Fusobacterium and ammonia production with related metabolic genes were greatly reduced with B-FOS and FOS treatment compared to the control group. These findings indicate that B-FOS differentially promotes butyrate production through the enhancement of butyrate-producing bacteria and their metabolic genes, and can be applied as a novel prebiotic compound in vivo.

scholarly journals Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation

2003 ◽  
Vol 62 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Herve M. Blottiere ◽  
Bruno Buecher ◽  
Jean-Paul Galmiche ◽  
Christine Cherbut
Keyword(s):  
Fatty Acids ◽  
Cell Proliferation ◽  
Short Chain Fatty Acids ◽  
Cyclin D3 ◽  
Short Chain ◽  
Intestinal Cell ◽  
Inhibitory Protein ◽  
Chain Fatty Acids

Short-chain fatty acids (SCFA), particularly butyrate, were shown to regulate cell proliferation in vitro and in vivo. Indeed, butyrate is the major fuel for colonic epithelial cells, and it can influence cell proliferation through the release of growth factors or gastrointestinal peptides such as gastrin, or through modulation of mucosal blood flow. Lastly, SCFA can act directly on genes regulating cell proliferation, and butyrate is the main SCFA to display such an effect. Butyrate inhibits histone deacetylase, which will allow histone hyperacetylation. Such hyperacetylation leads to transcription of several genes, including p21/Cipl. Moreover, it will allow cyclin D3 hyper-expression by inhibiting its degradation. The induction of the cyclin-dependent kinase inhibitory protein p21/Cipl accounts for cell arrest in the Gl phase of the cell cycle. However, in the absence of p21 other mechanisms are initiated, leading to inhibition of cell proliferation.

scholarly journals In vitro gas production and cinetical fermentation of a few local feed in Kupang East Nusa Tenggara

2021 ◽  
Vol 888 (1) ◽  
pp. 012077
Author(s):  
M M Kleden ◽  
E Hartati ◽  
M R D Ratu ◽  
G A Y Lestari ◽  
A E Manu
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Short Chain Fatty Acids ◽  
Fat Content ◽  
Gas Production ◽  
Carbohydrate Content ◽  
Short Chain ◽  
In Vitro Gas Production ◽  
Chain Fatty Acids

Abstract Research objectives were to evaluate and to analyze in vitro gas production and cinetical fermentation of Kupang’s local feed. The feed materials were dried and grinded for nutrient and gas produced analysis. Collected data were chemical composition, in vitro gas production, cinetical fermentation, short chain fatty acids, energy metabolic, and organic matter digestation. The result showed that the materials have different gas production. Commonly, the higher the carbohydrate content the higher the gas production; the higher the protein and the fat content the lower the gas production. Total gas productions of each material after 96 hours incubation were 53.88 ml/0.5 g DM for the high fat content feed, 92.26 and 159.25 ml/0.5 DM for the high protein and high carbohydrate content. Regarding total production, the fermented fraction gas productions were 44.662; 82.54 and 153.462 ml/0.5 g DM. The short chain fatty acids produced were 0.31; 0.51 and 1.28 mM. The feed organic matter digestations produced were 42.2; 55.91 and 68.8 % for each feed with a high content of fat, protein, and carbohydrate. Conclusively comparing with the protein and carbohydrate feed sources, the higher the fat content the lower the gas production, short chain fatty acids, and organic matter digestion.

scholarly journals Postnatal development of the myenteric glial network and its modulation by butyrate

2016 ◽  
Vol 310 (11) ◽  
pp. G941-G951 ◽  
Author(s):  
François Cossais ◽  
Tony Durand ◽  
Julien Chevalier ◽  
Marie Boudaud ◽  
Laetitia Kermarrec ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Enteric Neurons ◽  
Short Chain ◽  
Postnatal Maturation ◽  
Enteric Glial Cells ◽  
Postnatal Evolution ◽  
Chain Fatty Acids

The postnatal period is crucial for the development of gastrointestinal (GI) functions. The enteric nervous system is a key regulator of GI functions, and increasing evidences indicate that 1) postnatal maturation of enteric neurons affect the development of GI functions, and 2) microbiota-derived short-chain fatty acids can be involved in this maturation. Although enteric glial cells (EGC) are central regulators of GI functions, the postnatal evolution of their phenotype remains poorly defined. We thus characterized the postnatal evolution of EGC phenotype in the colon of rat pups and studied the effect of short-chain fatty acids on their maturation. We showed an increased expression of the glial markers GFAP and S100β during the first postnatal week. As demonstrated by immunohistochemistry, a structured myenteric glial network was observed at 36 days in the rat colons. Butyrate inhibited EGC proliferation in vivo and in vitro but had no effect on glial marker expression. These results indicate that the EGC myenteric network continues to develop after birth, and luminal factors such as butyrate endogenously produced in the colon may affect this development.

The additivity of predicted ATP yields from feedstuffs incubated in vitro with rumen fluid

2000 ◽  
Vol 2000 ◽  
pp. 25-25 ◽  
Author(s):  
A.R. Moss ◽  
C. Rymer ◽  
S. Fakhri ◽  
D.I. Givens
Keyword(s):  
Fatty Acids ◽  
Microbial Biomass ◽  
Rumen Fluid ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
The Individual ◽  
Micro Organisms ◽  
Chain Fatty Acids

Rumen micro-organisms ferment feeds to short chain fatty acids (SCFA) with the production of ATP. Measuring SCFA concentration in vitro could therefore be used to predict the yield of ATP in vitro. This estimate could then be used to predict the yield of synthesised microbial biomass. However, feeds may interact when they are incubated together, and so it is quite possible that ATP yield is not additive. The objectives of this experiment were therefore to determine whether the yield of total SCFA produced by feed mixtures was additive, and also whether the ATP yield of feed mixtures could be predicted from the calculated ATP yield of the individual feeds.

Short-chain fatty acids stimulate the migration of neutrophils to inflammatory sites

2009 ◽  
Vol 117 (9) ◽  
pp. 331-338 ◽  
Author(s):  
Marco A. R. Vinolo ◽  
Hosana G. Rodrigues ◽  
Elaine Hatanaka ◽  
Cristina B. Hebeda ◽  
Sandra H. P. Farsky ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Neutrophil Migration ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Mrna Levels ◽  
Air Pouch ◽  
Β2 Integrin ◽  
Chain Fatty Acids

SCFAs (short-chain fatty acids) are produced by anaerobic bacterial fermentation. Increased concentrations of these fatty acids are observed in inflammatory conditions, such as periodontal disease, and at sites of anaerobic infection. In the present study, the effect of the SCFAs acetate, propionate and butyrate on neutrophil chemotaxis and migration was investigated. Experiments were carried out in rats and in vitro. The following parameters were measured: rolling, adherence, expression of adhesion molecules in neutrophils (L-selectin and β2 integrin), transmigration, air pouch influx of neutrophils and production of cytokines [CINC-2αβ (cytokine-induced neutrophil chemoattractant-2αβ), IL-1β (interleukin-1β), MIP-1α (macrophage inflammatory protein-1α) and TNF-α (tumour necrosis factor-α)]. SCFAs induced in vivo neutrophil migration and increased the release of CINC-2αβ into the air pouch. These fatty acids increased the number of rolling and adhered cells as evaluated by intravital microscopy. SCFA treatment increased L-selectin expression on the neutrophil surface and L-selectin mRNA levels, but had no effect on the expression of β2 integrin. Propionate and butyrate also increased in vitro transmigration of neutrophils. These results indicate that SCFAs produced by anaerobic bacteria raise neutrophil migration through increased L-selectin expression on neutrophils and CINC-2αβ release.

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