scholarly journals Observation on the reproductive behavior and embryo of the daisy’s ricefish, Oryzias woworae in laboratory condition

2021 ◽  
Vol 919 (1) ◽  
pp. 012062
Author(s):  
M A Firmansyah ◽  
Mustahal ◽  
M B Syamsunarno ◽  
M Herjayanto

Abstract Oryzias woworae is an endemic ricefish from Muna Island, Southeast Sulawesi, traded as an ornamental fish. Information on the biology of these fish is very lacking, including their reproductive. These fish also face the threat of population decreasing in the wild. The research aimed to examine the spawning behavior and embryonic of O. woworae as the basis for aquaculture. Fish spawning was carried out using 1 male: 1 female ratio, which was repeated three times. The observed spawning behavior was pre-spawning, mating process, egg number, and morphology. Behavioral observations were carried out for five days of spawning. Observation of embryos was carried out to see the stages of embryo development until hatching. The results obtained on pre-spawning behavior were changes in body color and blackened fins in males, which did not occur in female fish. Males actively swim by approaching their dorsal and anal fins while chasing females. The female releases the eggs and is attached to the substrate, rapidly fertilized by the male fish. There is a phenomenon of females carrying eggs on the genital pore before being released to the substrate (non-pelvic brooder). Spawning only occurs during the day, and spawning is getting faster, which is at 09:00 AM on the fifth day. Oryzias woworae eggs have a diameter of 1.3 ± 0.2 mm, equipped with attaching and non-attaching filaments. Observations of O. woworae embryos showed that embryo hatching occurred at seven days 23 hours post-fertilization.

2020 ◽  
Vol 5 (2) ◽  
pp. 372-377
Author(s):  
Desrita ◽  
Vindy Rilani Manurung ◽  
Rizky Febriansyah Siregar ◽  
Rusdi Leidonald ◽  
Amanatul Fadhilah ◽  
...  

Based on statistical analysis from Dinas Kelautan Perikanan, Deli Serdang Regency in 2018, The capacity for freshwater aquaculture output was recorded at 1328.9 tons / year with a land area of 65.9 ha , which is a problem for the aquaculture community with the decreasing quantity of seed production, limited infrastructure and development management which is still conventional in Desa Perbarakan, Deli Serdang Regency, therefore it is important to implement the required application of science and technology, namely the implementation of semi-natural methods of spawning techniques of koi fish (Cyprinus carpio). Counseling, seminars and practices are the methods of tasks carried out. Extension activities were carried out by illustrating the conceptual awareness of the development of koi fish spawning strategies, the distribution of leaflets, the application of recognition technology, as well as tools and resources, including the dose of gonadotropin hormone (GnH) in broodfish and fish anaesthetic techniques. For female fish, the injected hormone level is 0.5 ml / kg and 0.1 ml / kg for male fish. The weight of the female fish is 1 kg tancho sanke doitsu, 0.5 ml/gr, and the weight of the male fish is 1 kg. At a dose of 0.1 ml / gr and 900 g of shiro, kohaku at a level of 0.09 ml / gr. The result is that the number of eggs is estimated at 10,000, and then the eggs hatch within 2-4 days. Boiled egg yolks are fed to the eggs which hatch into the larvae, then the larvae are transferred to the nursery pon after 10 days of age.


1998 ◽  
Vol 76 (3) ◽  
pp. 588-592 ◽  
Author(s):  
Margaret PM Burton ◽  
Shawn R Flynn

Male and female capelin (Mallotus villosus Müller) were captured in 1993 at the start of spawning and males were captured in 1995 at the end of the spawning period. All male fish from both years died within 6 weeks of capture. Spent males captured in the wild showed almost empty testes with very few residual sperm and no evidence of developing spermatocytes. All female fish survived the first 6 weeks and over 50% survived 20 weeks. Sampling of the surviving females after 20 weeks showed evidence of prior spawning and progressive development of oocytes for the next summer's spawning season. It was concluded that while males may be semelparous, females are inherently iteroparous.


1972 ◽  
Vol 50 (9) ◽  
pp. 1183-1188 ◽  
Author(s):  
M. Chen ◽  
G. Power

In samples taken monthly throughout the year the percentage of American smelt in Lake Ontario and Lake Erie containing cysts of Glugea hertwigi was 5.2% and 62.7% respectively. Sexual differences in incidence were observed, the significance of which was uncertain as results from the two lakes were contradictory.In male fish infection was almost entirely restricted to the digestive tract with few cysts in the liver, skin, and testes. In female fish the digestive tract and ovaries were similarly infected.Seasonal fluctuations in Glugea infection were obvious and seemed correlated with the gonadal cycle. In both sexes the highest parasite load corresponded with the onset of maturation.A striking difference in fecundity between the two smelt populations was attributed to the Glugea infection. In females parasite cysts replaced ovarian tissue, causing a reduction in the number of maturing eggs.


2018 ◽  
Vol 22 (2) ◽  
pp. 183-190
Author(s):  
Krishna Adhikari ◽  
Laxman Khanal ◽  
Mukesh Kumar Chalise

The population status of Assamese monkey (Macaca assamensis) (McClelland 1840) and its interaction with the local people is poorly documented in Nepal. In 2014, we studied the population status, diurnal time budget and human-monkey conflict in Ramdi, Nepal by direct count, scan sampling and questionnaire survey methods, respectively. Two troops of Assamese monkey having total population of 48 with the mean troop size of 24 individuals were recorded in the study area. The group density was 0.33 groups / km² with a population density of 6 individuals/ km². The male to female adult sex ratio was 1:1.75 and the infant to female ratio was 0.85. The provisioned troop of Assamese monkey in Ramdi spent less time in feeding and movement while higher time was invested in resting and grooming than the nonspecific populations in the wild habitats. The human-monkey conflict is high in Ramdi, primarily due to the crop-raiding from fields and even the storage; the major crops raided are maize, vegetables, pulses and fruits. The higher reproductive success in terms of infant to adult female ratio, increased human-monkey conflicts and lesser investment of the diurnal time in foraging and feeding by Assamese monkey troop of Ramdi might be associated with the food provisioning. Journal of Institute of Science and TechnologyVolume 22, Issue 2, January 2018, Page: 183-190


2021 ◽  
Vol 21 (2) ◽  
pp. 167-184
Author(s):  
Friesland Tuapetel

Eco-biological information of Cheilopogon abei in Geser Streit is unknown. The objective of this research is to observe the sex ratio, first size maturity, gonad maturity and gonado somatic index (GSI) with fecundity of flying fish Ch abei in Geser East Seram Strait waters. Sampling was done for ten months started from February to November 2018, using gill net measuring 1.50 inch. All fish samples were measured in fork length and weighted so dissected to make observations on the level of gonad maturity and the number of eggs in female fish. A total of 682 flying fish was found during the study with a fork length range of 182.6-243.3 mm and a weight of 73.98-115.45 g. The results of the analysis showed that the proportion of the number of male fish was less than that of the female fish for almost every month of observation. The first size gonad mature of male and female is 210.5 mm and 214.1 mm FL. The gonad maturity index of male fish ranges from 0,963-7,967 and female fish ranges 1,315-8,069, the total fecundity is 2321-9438 eggs. Gonads of ripe fish in each month of observation. The spawning peak of Ch abei takes place in June-July and it is hoped that no arrests will be made in both months, to ensure the sustainability of the stock.


2021 ◽  
Vol 57 (4) ◽  
pp. 93-96
Author(s):  
Dileshwari Ratre ◽  
S. N. Ojha ◽  
Arpita Sharma ◽  
Ramasubramanian V ◽  
Rajpal Yadav

The ratio of fish farmers to fisheries extension professionals was 1:1000 which is too low. Para-extension functionaries can fill up this gap in the fisheries sector to improve the production and productivity of fishers. The opinion leaders from the local fish farmers community will help identify fish farmers who can further transfer the knowledge concerning how to fish to others through their diffusion network. This study was undertaken to analyze gender roles in fish farming and their relation with opinion leaders. It was observed that 90% of male fish farmers had control of fisheries/family resources, and 97 per cent of fish farmers were found to have contact with male opinion leaders. At the same time, 33% of female fish farmers were contacted by shelf help groups and local progressive farmers. The study shows that the opinion leaders influenced the fish farmers’ diffusion network and their knowledge about how to fish. To involve women in the fisheries sector, empower women, there is also a need to select opinion leaders preferred by women fish farmers.


2020 ◽  
Vol 26 (2) ◽  
pp. 97
Author(s):  
Melta R. Fahmi ◽  
Eni Kusrini ◽  
Erma P. Hayuningtiyas ◽  
Shofihar Sinansari ◽  
Rudhy Gustiano

The wild betta fish is a potential ornamental fish export commodity normally caught by traders or hobbyists in the wild. However, the population of wild betta in nature has declined and become a threat for their sustainability. This research was conducted to analyze the genetic diversity, phylogenetic relationships, and molecular identification through DNA COI gene sequence of Indonesian wild betta fish. A total of 92 wild betta fish specimens were collected in this study. Amplification of COI genes was carried out using Fish F1, Fish R1, Fish F2, and Fish R2 primers. The genetic diversity and phylogenetic relationships were analyzed using MEGA version 5 software program. Species identification of the specimen was conducted using BLAST program with 98-100% similarity value of the DNA sequences to indicate the same species. Phylogenetic tree construction showed seven monophyletic clades and showed that Betta smaragdina was the ancestral species of genus Betta in Indonesian waters. Genetic distance among species ranged from 0.02 to 0.30, whereas intra-species genetic distance ranged from 0 to 6.54.


1992 ◽  
Vol 263 (6) ◽  
pp. R1241-R1247 ◽  
Author(s):  
B. S. Washburn ◽  
M. L. Bruss ◽  
E. H. Avery ◽  
R. A. Freedland

Reports of changes in carbohydrate metabolism during vitellogenesis in fish prompted an investigation of the effects of estrogen on glucose utilization in rainbow trout. Estrogen pellets were implanted in both female and male fish, and a third group of male fish was given a sham operation. After cannulation of the dorsal aorta, D-[1-3H]glucose and 2-deoxy-D-[U-14C]glucose were injected into the fish to observe whole animal and tissue glucose use. We found that estrogen does not affect glucose turnover rate or transit time but causes a decrease in plasma glucose concentration and size of the glucose mixing pool. Adipose tissue in female fish utilized glucose at a higher rate than sham fish. Ovarian tissue used more glucose per kilogram of body weight than the testes of the male fish. Regardless of treatment, brain had the highest rate of glucose consumption per gram of tissue, followed by gonads and red blood cells. Muscle and adipose tissue utilized only small amounts (< 1 nmol.g tissue-1.min-1) of glucose. We conclude that an increase in the rate of whole body glucose use is not responsible for the fall in plasma glucose caused by estrogen and seen during vitellogenesis.


2005 ◽  
Vol 79 (1) ◽  
pp. 91-94 ◽  
Author(s):  
M. Schabuss ◽  
M. Gemeiner ◽  
A. Gleiß ◽  
J.W. Lewis ◽  
I Miller ◽  
...  

AbstractEuropean chub Leuciscus cephalus collected from five localities in the lowland and subalpine regions of Austria were analysed for oestrogenic effects of endocrine-disrupting chemicals and the presence of the plerocercoid of the tapeworm Ligula intestinalis. Of 1494 chub analysed, only seven (six males, one female) were found to be infected with single, but large plerocercoids up to 15 cm in length. Ligula-infected fish showed comparatively immature gonads, as demonstrated by the gonadosomatic index and gamete developmental stages. Plasma levels of the egg precursor protein vitellogenin also showed concentrations ranging below the detection limit. The present results indicate that chub infected with L. intestinalis and exposed to exogenous oestrogenic compounds can result in reduced gonadal maturation and produce false oestrogen-positive diagnoses in male fish. For plasma vitellogenin levels, L. intestinalis infections can result in false oestrogen-negative diagnoses in male and female fish.


2008 ◽  
Vol 7 (1) ◽  
pp. 33
Author(s):  
H. Arfah ◽  
O. Carman

<p>The female of Thai catfish <em>Pangasionodon hypopthalmus</em> had higher growth rate, about 25-30% than male fish, especially on growth phase-2 when the fish get sexually mature. Monosex female fish culture system can increase production efficiency in term of time and cost.  Experiment was performed to get male homogametic (XX) that will be used as functional male to produce female monosex (XX) population Result of hormonal and temperature manipulation on larvae shown that the highest percentage of male (67.7%) was obtained by 5 mg/liter 17-α-metiltestosteron treatment with temperature 33<sup>o</sup>C. Good temperature for larva rearing was 30<sup>o</sup>C. The result of fertility test on male fish was fertile, but progeny test was not performed homogametic (XX) character yet.</p> <p>Keywords: hormonal manipulation, male homogametic XX, monosex, <em>Pangasionodon hypopthalmus</em></p> <p> </p> <p>ABSTRAK</p> <p>Ikan patin <em>Pangasionodon hypopthalmus </em>betina memiliki laju pertumbuhan lebih cepat sekitar 25-30% daripada yang jantan, terutama pada fase pertumbuhan II saat ikan mulai matang kelamin. Budidaya ikan dengan sistem kultur monoseks ikan betina diduga akan meningkatkan efisiensi produksi dari segi waktu dan biaya. Penelitian ini bertujuan mendapatkan jantan homogametik (XX) yang akan dimanfaatkan sebagai jantan fungsional untuk menghasilkan populasi monoseks betina (XX). Hasil manipulasi hormon dan suhu terhadap larva ikan patin menunjukkan bahwa presentase kelamin jantan tertinggi (67,7%) terjadi dengan dosis perendaman dalam hormone 17-α metiltestosteron 5 mg/liter dengan suhu inkubasi 33<sup>o</sup>C. Suhu yang baik untuk pemeliharaan larva ialah 30<sup>o</sup>C. Hasil uji fertilitas terhadap induk jantan bersifat fertile, namun uji progeny belum dapat menunjukkan sifat homogametik (XX).</p> <p>Kata kunci : manipulasi hormonal, jantan homogametik (XX), monoseks betina, ikan patin, <em>Pangasionodon hypopthalmus</em></p>


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