scholarly journals Hormonal and Temperature Manipulation to Produce Male Homogametic (XX) in Developing Female Monosex Culture of Thai Catfish Pangasionodon hypopthalmus

2008 ◽  
Vol 7 (1) ◽  
pp. 33
Author(s):  
H. Arfah ◽  
O. Carman

<p>The female of Thai catfish <em>Pangasionodon hypopthalmus</em> had higher growth rate, about 25-30% than male fish, especially on growth phase-2 when the fish get sexually mature. Monosex female fish culture system can increase production efficiency in term of time and cost.  Experiment was performed to get male homogametic (XX) that will be used as functional male to produce female monosex (XX) population Result of hormonal and temperature manipulation on larvae shown that the highest percentage of male (67.7%) was obtained by 5 mg/liter 17-α-metiltestosteron treatment with temperature 33<sup>o</sup>C. Good temperature for larva rearing was 30<sup>o</sup>C. The result of fertility test on male fish was fertile, but progeny test was not performed homogametic (XX) character yet.</p> <p>Keywords: hormonal manipulation, male homogametic XX, monosex, <em>Pangasionodon hypopthalmus</em></p> <p> </p> <p>ABSTRAK</p> <p>Ikan patin <em>Pangasionodon hypopthalmus </em>betina memiliki laju pertumbuhan lebih cepat sekitar 25-30% daripada yang jantan, terutama pada fase pertumbuhan II saat ikan mulai matang kelamin. Budidaya ikan dengan sistem kultur monoseks ikan betina diduga akan meningkatkan efisiensi produksi dari segi waktu dan biaya. Penelitian ini bertujuan mendapatkan jantan homogametik (XX) yang akan dimanfaatkan sebagai jantan fungsional untuk menghasilkan populasi monoseks betina (XX). Hasil manipulasi hormon dan suhu terhadap larva ikan patin menunjukkan bahwa presentase kelamin jantan tertinggi (67,7%) terjadi dengan dosis perendaman dalam hormone 17-α metiltestosteron 5 mg/liter dengan suhu inkubasi 33<sup>o</sup>C. Suhu yang baik untuk pemeliharaan larva ialah 30<sup>o</sup>C. Hasil uji fertilitas terhadap induk jantan bersifat fertile, namun uji progeny belum dapat menunjukkan sifat homogametik (XX).</p> <p>Kata kunci : manipulasi hormonal, jantan homogametik (XX), monoseks betina, ikan patin, <em>Pangasionodon hypopthalmus</em></p>

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 28
Author(s):  
Ludmila Kalčáková ◽  
Matej Pospiech ◽  
Bohuslava Tremlová ◽  
Zdeňka Javůrková ◽  
Irina Chernukha

To increase production efficiency of meat products, milk protein additives are often used. Despite a number of advantages, use of dairy ingredients involves a certain risk, namely the allergenic potential of milk proteins. A number of methods have been developed to detect milk-origin raw materials in foodstuffs, including immunological reference methods. This study presents newly developed immunohistochemical (IHC) methods for casein detection in meat products. Casein was successfully detected directly in meat products where sensitivity was determined at 1.21 and specificity at 0.28. The results obtained from the IHC were compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) and there was no statistically significant difference between the IHC and ELISA methods (p > 0.05). The correspondence between the methods was 72% in total. The highest correspondence was reached in frankfurters (90%), the lowest in canned pâté (44%).


1972 ◽  
Vol 50 (9) ◽  
pp. 1183-1188 ◽  
Author(s):  
M. Chen ◽  
G. Power

In samples taken monthly throughout the year the percentage of American smelt in Lake Ontario and Lake Erie containing cysts of Glugea hertwigi was 5.2% and 62.7% respectively. Sexual differences in incidence were observed, the significance of which was uncertain as results from the two lakes were contradictory.In male fish infection was almost entirely restricted to the digestive tract with few cysts in the liver, skin, and testes. In female fish the digestive tract and ovaries were similarly infected.Seasonal fluctuations in Glugea infection were obvious and seemed correlated with the gonadal cycle. In both sexes the highest parasite load corresponded with the onset of maturation.A striking difference in fecundity between the two smelt populations was attributed to the Glugea infection. In females parasite cysts replaced ovarian tissue, causing a reduction in the number of maturing eggs.


Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 564
Author(s):  
Supakorn Potijun ◽  
Chonlada Yaisamlee ◽  
Anchalee Sirikhachornkit

Microalgae have long been used for the commercial production of natural colorants such as carotenoids and chlorophyll. Due to the rising demand for carotenoids and other natural products from microalgae, strategies to increase production efficiency are urgently needed. The production of microalgal biorefineries has been limited to countries with moderate climates. For countries with cooler climates and less daylight, methodologies for the efficient production of microalgal biorefineries need to be investigated. Algal strains that can be safely consumed as whole cells are also attractive alternatives for developing as carotenoid supplements, which can also contain other compounds with health benefits. Using such strains helps to eliminate the need for hazardous solvents for extraction and several other complicated steps. In this study, the mesophilic green alga Chlamydomonas reinhardtii was employed to study the effects of cold stress on cell physiology and the production of pigments and storage compounds. The results showed that temperatures between 10 and 20 °C induced carotenoid and chlorophyll accumulation in the wild-type strain of C. reinhardtii. Interestingly, the increased level of carotenoids suggested that they might play a crucial role in cold stress acclimation. A temperature of 15 °C resulted in the highest carotenoid and chlorophyll productivity. At this temperature, carotenoid and chlorophyll productivity was 2 times and 1.3 times higher than at 25 °C, respectively. Subjecting a mutant defective in lutein and zeaxanthin accumulation to cold stress revealed that these two carotenoids are not essential for cold stress survival. Therefore, cold temperature could be used as a strategy to induce and increase the productivity of pigments in C. reinhardtii.


2012 ◽  
Vol 628 ◽  
pp. 206-210 ◽  
Author(s):  
Jia Liang Zhang ◽  
Bei Zhi Li ◽  
Xin Chao Zhang ◽  
Qing Xia Wang

Friction stir welding processes involve many variables. Engineers and operators often find it difficult to effectively design or control it. The objective of this work is to develop a friction stir welding platform of thin plates to improve welding quality and to increase production efficiency. The study is conducted by using finite element modeling and temperature field analysis technology to obtain optimization parameters, and using virtual instrument, multi-sensor data fusion to monitor the force of the stirring spindle. Experiment results show that the developed platform can reach the requirements of processing quality and is cost-effective.


2014 ◽  
Vol 30 (1) ◽  
pp. 1-13 ◽  
Author(s):  
M. Sahraei

Continuous genetic selection and improvement in nutrition have led to a very fast growth rate in modern strains of broiler chickens. Metabolic disorders such as ascits, sudden death syndrome and leg problems are related to a rapid early growth rate in poultry, especially in broilers, and their incidence can be decreased by slowing early growth. The use of management tools to reduce metabolic disorders that rely primarily on decreasing feed consumption, The feed restriction programs is on of the main techniques in growth curve manipulation for increasing production efficiency in broiler chicken in alleviate the incidence of some metabolic disorders and can be used to reduction the unfavorable effects of fast growth rate in broiler chicken production industry, and could be profitable in broiler chickens production efficiency. This article implicated on new findings in about different feed restriction programs effects on these problems in broiler chickens.


2021 ◽  
Vol 21 (2) ◽  
pp. 167-184
Author(s):  
Friesland Tuapetel

Eco-biological information of Cheilopogon abei in Geser Streit is unknown. The objective of this research is to observe the sex ratio, first size maturity, gonad maturity and gonado somatic index (GSI) with fecundity of flying fish Ch abei in Geser East Seram Strait waters. Sampling was done for ten months started from February to November 2018, using gill net measuring 1.50 inch. All fish samples were measured in fork length and weighted so dissected to make observations on the level of gonad maturity and the number of eggs in female fish. A total of 682 flying fish was found during the study with a fork length range of 182.6-243.3 mm and a weight of 73.98-115.45 g. The results of the analysis showed that the proportion of the number of male fish was less than that of the female fish for almost every month of observation. The first size gonad mature of male and female is 210.5 mm and 214.1 mm FL. The gonad maturity index of male fish ranges from 0,963-7,967 and female fish ranges 1,315-8,069, the total fecundity is 2321-9438 eggs. Gonads of ripe fish in each month of observation. The spawning peak of Ch abei takes place in June-July and it is hoped that no arrests will be made in both months, to ensure the sustainability of the stock.


2021 ◽  
Author(s):  
weibin zeng ◽  
Lei An ◽  
Yanping Wang ◽  
Shuai Gao ◽  
Yusheng Qin ◽  
...  

Abstract Background: Estrogen could limit the nondominant follicles development after the first deviation by inhibition of the FSH secretion through the negative feedback loop, which ensure that the number of dominant follicles would be in a moderate level. Methods: The objective of the present study was to evaluate the effect of estradiol benzoate (EB) on inhibiting the development of nondominant follicles and inducing twin calves in beef heifers. Beef heifers were synchronized using an estradiol (E2)- plus- progesterone (P4)- based and superovulated using small dose follicle- stimulating hormone (FSH) protocol. From days 6.5 to 7.5 every heifer was treated with variety dose of estradiol benzoate (EB) for 3 times with 12 h intervals to eliminate the excess number of dominant follicles. Results: The diameters of the two largest follicles (F1 and F2) continually increased from day 3.5 to day 10. However, the growth rate was constrained by exogenous EB, and the degree of suppression was greatest in the 0.5 mg EB treatment compared with other treatments. As a result, the number of large follicles (≥ 10 mm) was also reduced along with the dose of EB increased. The double/triple ovulations rate, pregnancy rate and twin were all demonstrate the highest in 0.2 mg EB treatment group than in other treatments. Conclusions:The present study describes an efficient protocol that can be used to stimulate the development of a small number of dominant follicles i.e. 2-3 at the deviation stage through a FSH and 0.2 mg EB combine treatment, which can further result in the production of two calves. The appropriate dose of EB treatment during FSH induced superovulation procedure could limit the number of dominant follicles development and eventually increase the calf production efficiency.


2020 ◽  
Vol 5 (2) ◽  
pp. 372-377
Author(s):  
Desrita ◽  
Vindy Rilani Manurung ◽  
Rizky Febriansyah Siregar ◽  
Rusdi Leidonald ◽  
Amanatul Fadhilah ◽  
...  

Based on statistical analysis from Dinas Kelautan Perikanan, Deli Serdang Regency in 2018, The capacity for freshwater aquaculture output was recorded at 1328.9 tons / year with a land area of 65.9 ha , which is a problem for the aquaculture community with the decreasing quantity of seed production, limited infrastructure and development management which is still conventional in Desa Perbarakan, Deli Serdang Regency, therefore it is important to implement the required application of science and technology, namely the implementation of semi-natural methods of spawning techniques of koi fish (Cyprinus carpio). Counseling, seminars and practices are the methods of tasks carried out. Extension activities were carried out by illustrating the conceptual awareness of the development of koi fish spawning strategies, the distribution of leaflets, the application of recognition technology, as well as tools and resources, including the dose of gonadotropin hormone (GnH) in broodfish and fish anaesthetic techniques. For female fish, the injected hormone level is 0.5 ml / kg and 0.1 ml / kg for male fish. The weight of the female fish is 1 kg tancho sanke doitsu, 0.5 ml/gr, and the weight of the male fish is 1 kg. At a dose of 0.1 ml / gr and 900 g of shiro, kohaku at a level of 0.09 ml / gr. The result is that the number of eggs is estimated at 10,000, and then the eggs hatch within 2-4 days. Boiled egg yolks are fed to the eggs which hatch into the larvae, then the larvae are transferred to the nursery pon after 10 days of age.


2021 ◽  
Vol 57 (4) ◽  
pp. 93-96
Author(s):  
Dileshwari Ratre ◽  
S. N. Ojha ◽  
Arpita Sharma ◽  
Ramasubramanian V ◽  
Rajpal Yadav

The ratio of fish farmers to fisheries extension professionals was 1:1000 which is too low. Para-extension functionaries can fill up this gap in the fisheries sector to improve the production and productivity of fishers. The opinion leaders from the local fish farmers community will help identify fish farmers who can further transfer the knowledge concerning how to fish to others through their diffusion network. This study was undertaken to analyze gender roles in fish farming and their relation with opinion leaders. It was observed that 90% of male fish farmers had control of fisheries/family resources, and 97 per cent of fish farmers were found to have contact with male opinion leaders. At the same time, 33% of female fish farmers were contacted by shelf help groups and local progressive farmers. The study shows that the opinion leaders influenced the fish farmers’ diffusion network and their knowledge about how to fish. To involve women in the fisheries sector, empower women, there is also a need to select opinion leaders preferred by women fish farmers.


2020 ◽  
Vol 4 (1) ◽  
pp. 9
Author(s):  
Dadang Hermawan ◽  
Andy Hardianto ◽  
Arief Rizki Fadhillah

Cassava or cassava (Manihot esculenta Crantz) is one of Indonesia's local carbohydrate sources which ranks third largest after rice and corn. One of the uses of cassava is cassava crackers (samiler). Cassava crackers (samiler) are very popular snacks among the people, made from cassava, thin round in shape and taste delicious. Production Capacity of Karya Lestari Jaya's UKM in producing cassava crackers (samiler) in 1 day is ± 20 kg of material or 5 kg of dried cassava crackers (samiler). In one month cassava cracker orders (samiler) can reach ± 80-120 kg. The production process of cassava crackers (samiler) goes through several stages, including: raw material process, printing process, steam process, drying process and packaging process. The problem that can be identified in the field of production is the limited equipment in the packaging process and weighing the samiler cassava crackers. Karya Lestari Jaya's UKM partners in calculating product prices per package have not met the break even point (BEP) standard, so it cannot be maximized in business management. From the above conditions, the method and results of this service are providing technology transfer by providing vaccum sealer machines, continuous band sealers, and digital scales as well as training in the use of tools and training in Break Even Point (BEP) so as to increase production efficiency of cassava crackers (samiler). ABSTRAKSingkong atau ubikayu (Manihot esculenta Crantz) merupakan salah satu sumber  karbohidrat lokal Indonesia yang menduduki urutan ketiga terbesar setelah padi dan jagung. Salah satu pemanfaatan singkong adalah kerupuk singkong (samiler).  Kerupuk singkong (samiler) adalah makanan ringan yang sangat populer dikalangan masyarakat, terbuat dari singkong , berbentuk bundar tipis dan rasanya gurih. Kapasitas Produksi UKM Karya Lestari Jaya dalam memproduksi kerupuk singkong (samiler) dalam 1 hari sebanyak ± 20 kg bahan atau 5 kg kerupuk singkong (samiler) kering. Dalam satu bulan pesanan kerupuk singkong (samiler) dapat mencapai ± 80-120 kg. Proses Produksi kerupuk singkong (samiler) melalui beberapa tahapan, antara lain : proses bahan baku, proses pencetakan, proses steam, proses penjemuran dan proses pengemasan. Permasalahan yang dapat diidentifikasi dalam bidang produksi adalah keterbatasan peralatan dalam proses packaging dan penimbangan kerupuk singkong samiler. Mitra UKM Karya Lestari Jaya dalam melakukan perhitungan harga produk per kemasan belum memenuhi standar break even point (BEP), sehingga tidak dapat maksimal dalam pengelolaan usaha. Dari kondisi diatas maka metode dan hasil dari pengabdian ini adalah memberikan transfer teknologi dengan memberikan Mesin vaccum sealer, continuous band sealer, dan timbangan digital serta melakukan pelatihan penggunaan alat dan pelatihan Break Even Point (BEP) sehingga meningkatkan efisiensi produksi Kerupuk singkong (samiler).


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