3D printing of step-gradient nanocomposite hydrogels for controlled cell migration

2019 ◽  
Vol 11 (4) ◽  
pp. 045015 ◽  
Author(s):  
Andisheh Motealleh ◽  
Betül Çelebi-Saltik ◽  
Nihal Ermis ◽  
Sacha Nowak ◽  
Ali Khademhosseini ◽  
...  
Keyword(s):  
Cell Migration ◽  
3D Printing ◽  
Nanocomposite Hydrogels

scholarly journals Printable Alginate Hydrogels with Embedded Network of Halloysite Nanotubes: Effect of Polymer Cross-Linking on Rheological Properties and Microstructure

Polymers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 4130
Author(s):  
Svetlana A. Glukhova ◽  
Vyacheslav S. Molchanov ◽  
Boris V. Lokshin ◽  
Andrei V. Rogachev ◽  
Alexey A. Tsarenko ◽  
...  
Keyword(s):  
3D Printing ◽  
Rheological Properties ◽  
Shear Thinning ◽  
Halloysite Nanotubes ◽  
Polymer Chains ◽  
Cross Linking ◽  
Saxs Data ◽  
Nanocomposite Hydrogels ◽  
Embedded Network ◽  
The Cross

Rapidly growing 3D printing of hydrogels requires network materials which combine enhanced mechanical properties and printability. One of the most promising approaches to strengthen the hydrogels consists of the incorporation of inorganic fillers. In this paper, the rheological properties important for 3D printability were studied for nanocomposite hydrogels based on a rigid network of percolating halloysite nanotubes embedded in a soft alginate network cross-linked by calcium ions. Particular attention was paid to the effect of polymer cross-linking on these properties. It was revealed that the system possessed a pronounced shear-thinning behavior accompanied by a viscosity drop of 4–5 orders of magnitude. The polymer cross-links enhanced the shear-thinning properties and accelerated the viscosity recovery at rest so that the system could regain 96% of viscosity in only 18 s. Increasing the cross-linking of the soft network also enhanced the storage modulus of the nanocomposite system by up to 2 kPa. Through SAXS data, it was shown that at cross-linking, the junction zones consisting of fragments of two laterally aligned polymer chains were formed, which should have provided additional strength to the hydrogel. At the same time, the cross-linking of the soft network only slightly affected the yield stress, which seemed to be mainly determined by the rigid percolation network of nanotubes and reached 327 Pa. These properties make the alginate/halloysite hydrogels very promising for 3D printing, in particular, for biomedical purposes taking into account the natural origin, low toxicity, and good biocompatibility of both components.

All-in-One Cellulose Nanocrystals for 3D Printing of Nanocomposite Hydrogels

2018 ◽  
Vol 130 (9) ◽  
pp. 2377-2380 ◽  
Author(s):  
Jieping Wang ◽  
Annalisa Chiappone ◽  
Ignazio Roppolo ◽  
Feng Shao ◽  
Erika Fantino ◽  
...  
Keyword(s):  
3D Printing ◽  
Cellulose Nanocrystals ◽  
Nanocomposite Hydrogels

Directed vertical cell migration via bifunctionalized nanomaterials in 3D step-gradient nanocomposite hydrogels

2020 ◽  
Vol 8 (20) ◽  
pp. 5628-5637
Author(s):  
Andisheh Motealleh ◽  
Nermin S. Kehr
Keyword(s):  
Cell Migration ◽  
Healthy Cell ◽  
Nanocomposite Hydrogels ◽  
Cancerous Cells ◽  
Vertical Cell ◽  
Gradient Scaffolds

Step-gradient scaffolds promote healthy cell migration, while inhibit the migration of cancerous cells in the XZ plane of the 2GradNS.

scholarly journals 3D printing of biomimetic microstructures for cancer cell migration

2013 ◽  
Vol 16 (1) ◽  
pp. 127-132 ◽  
Author(s):  
Tina Qing Huang ◽  
Xin Qu ◽  
Justin Liu ◽  
Shaochen Chen
Keyword(s):  
Cell Migration ◽  
3D Printing ◽  
Cancer Cell ◽  
Cancer Cell Migration

scholarly journals pH-Dependent Nanodiamonds Enhance the Mechanical Properties of 3D-Printed Hyaluronic Acid Nanocomposite Hydrogels

2020 ◽  
Author(s):  
Dae Gon Lim ◽  
Eunah Kang ◽  
Seong Hoon Jeong
Keyword(s):  
Mechanical Properties ◽  
Hyaluronic Acid ◽  
3D Printing ◽  
Compressive Stress ◽  
Critical Role ◽  
Weight Ratio ◽  
Compressive Force ◽  
Dependent Manner ◽  
Nanocomposite Hydrogels ◽  
Ph Dependent

Abstract Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality. 3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt % resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites. These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner. HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40 fold (0.02%: 236.18 kPa) and 1.37 fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt % and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt %) at pH 8 was mechanically enhanced 1.29 fold, compared to that of HA/ND-OH (0.04 wt %) at pH 7. These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel. Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.

scholarly journals 3D Printing of Alginate-Natural Clay Hydrogel-Based Nanocomposites

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 211
Author(s):  
Rebeca Leu Leu Alexa ◽  
Raluca Ianchis ◽  
Diana Savu ◽  
Mihaela Temelie ◽  
Bogdan Trica ◽  
...  
Keyword(s):  
3D Printing ◽  
Composite Scaffold ◽  
Infrared Spectrometry ◽  
Micro Computed Tomography ◽  
Buffer Solution ◽  
Nanocomposite Hydrogels ◽  
Crosslinking Process ◽  
Type Selection ◽  
Biological Studies ◽  
3D Printed

Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine. The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration. In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks. Using the extrusion-based nozzle, the nanocomposites inks were printed to obtain 3D multilayered scaffolds. To observe the behavior induced by each type of clay on alginate-based inks, rheology studies were performed on composite inks. The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT). The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time. Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules. This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs. Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.

All-in-One Cellulose Nanocrystals for 3D Printing of Nanocomposite Hydrogels

2018 ◽  
Vol 57 (9) ◽  
pp. 2353-2356 ◽  
Author(s):  
Jieping Wang ◽  
Annalisa Chiappone ◽  
Ignazio Roppolo ◽  
Feng Shao ◽  
Erika Fantino ◽  
...  
Keyword(s):  
3D Printing ◽  
Cellulose Nanocrystals ◽  
Nanocomposite Hydrogels

scholarly journals pH-Dependent Nanodiamonds Enhance the Mechanical Properties of 3D-Printed Hyaluronic Acid Nanocomposite Hydrogels

2020 ◽  
Author(s):  
Dae Gon Lim ◽  
Eunah Kang ◽  
Seong Hoon Jeong
Keyword(s):  
Mechanical Properties ◽  
Hyaluronic Acid ◽  
3D Printing ◽  
Compressive Stress ◽  
Critical Role ◽  
Biological Tissues ◽  
Dependent Manner ◽  
Inorganic Particles ◽  
Nanocomposite Hydrogels ◽  
Ph Dependent

Abstract Background: Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality. Mechanically enhanced biomaterials composited with diverse nanophase inorganic particles are challenging because metastatic materials mimicking biological tissues require softness, biocompatibility, strength, and structurally compatible elasticity. Herein, nanodiamonds (NDs) have been chosen due to their hydrophilicity and pH-dependent surface functionality, along with their convenient chemical modalityResults: 3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt % resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites. These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner. HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40 fold (236.18 kPa) and 1.37 fold (616.72 kPa) the compressive stress at the composition of 0.02 wt % and 0.04 wt %, respectively, compared to those of ND-COOH (168.31, 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt %) at pH 8 was mechanically enhanced 1.29 fold, compared to that of HA/ND-OH (0.04 wt %) at pH 7. These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Conclusion: Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel. Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.

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