Structural, optical, and potential broad-spectrum antibacterial properties of CuO-Ag nanoparticles biosynthesized using the extract of Diospyros discolor Willd

2021 ◽  
Vol 12 (4) ◽  
pp. 045007
Author(s):  
Windri Handayani ◽  
Cuk Imawan ◽  
Aminah Umar ◽  
Yasman ◽  
Retno Yunilawati ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Broad Spectrum ◽  
Ag Nanoparticles ◽  
Antimicrobial Agents ◽  
Bimetallic Nanoparticles ◽  
Antibacterial Properties ◽  
Ag Nps ◽  
New Material ◽  
Spectrum Activity ◽  
Absorbance Peak

Abstract The development of new material properties for antimicrobial agents to address the problem of antibiotic resistance is currently being explored. Silver and copper exhibit oligodynamic properties, which inhibit the growth of microorganisms. These properties are stronger at the nanoscale than the macroscale, especially for bimetallic nanoparticles (NPs). In this study, we synthesized bimetallic CuO and Ag NPs using Diospyros discolor Willd. (Velvet apple) leaf extract with three different ratios of Cu and Ag precursors: 1:0.01, 1:0.1, and 1:1 (v/v). In addition, the optical properties of these NPs were characterised using UV-Vis spectrophotometer, which showed the typical absorbance peak in the range of 300–400 nm. The size and shape of the CuO and Ag nanoparticles were analysed using a scanning electron microscope (SEM) and transmission electron microscope (TEM) images. The SEM and TEM images showed leaf-shaped CuO nanoplates and spherical AgNPs. Furthermore, the crystalline properties were analysed using x-ray diffraction (XRD). This study produced CuO-Ag nanoclusters with a particular morphology, size, and composition. The antibacterial assay showed potential broad-spectrum activity for Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) as well as Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa).

scholarly journals Extracellular probiotic lipase capped silver nanoparticles as highly efficient broad spectrum antimicrobial agents

RSC Advances ◽  
2018 ◽  
Vol 8 (55) ◽  
pp. 31358-31365 ◽  
Author(s):  
Imran Khan ◽  
Nivetha Sivasankaran ◽  
Ravikiran Nagarjuna ◽  
Ramakrishnan Ganesan ◽  
Jayati Ray Dutta
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Broad Spectrum ◽  
Antimicrobial Agents ◽  
Ag Nps ◽  
Highly Efficient

A series of Ag NPs capped with different concentrations of probiotic lipase have been synthesized. The obtained Ag NPs exhibited efficient broad spectrum antimicrobial activity at concentrations much lower than the LC50values of zebrafish.

Green Methods for Preparing Ag Nanoparticles Decorated Non-Woven Fabrics with High Antibacterial Efficiency

2020 ◽  
Vol 984 ◽  
pp. 75-81
Author(s):  
Zhi Jin ◽  
Jin Ru Ji ◽  
Xi Ya Wang ◽  
Chao Fan Shi ◽  
Xi Wen Zhang
Keyword(s):  
Plasma Treatment ◽  
Ag Nanoparticles ◽  
Chemical Reduction ◽  
Antibacterial Properties ◽  
Chemical Precipitation ◽  
Woven Fabrics ◽  
Dbd Plasma ◽  
Step Method ◽  
Ag Nps ◽  
Antibacterial Efficiency

The present study involves preparation of Ag nanoparticles (Ag NPs) deposited on non-woven fabrics by a two-step method including the chemical precipitation of Ag2O nanoparticles on the fabrics followed by in situ reducing of the precursor into Ag NPs by different reductive processes including chemical reduction, photo-reduction and dielectric barrier discharge (DBD) cold plasma treatment. The morphology as well as the antibacterial efficiency of the Ag NPs deposited on non-woven fabrics are also evaluated. The Ag NPs-modified fabrics prepared by DBD plasma treatment exhibit significant antibacterial properties against both Escherichia coli and Staphylococcus aureus, and accordingly have potential applications in the healthcare and medicine fields.

scholarly journals Liver Tissue Regeneration using Nano Silver impregnated Sodium Alginate/PVA Composite Nanofibres

2020 ◽  
Vol 2 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Manikandan G ◽  
Yuvashree M ◽  
Sangeetha A ◽  
Bhuvana K P ◽  
Sanjay K Nayak
Keyword(s):  
Antibacterial Activity ◽  
Sodium Alginate ◽  
Ag Nanoparticles ◽  
Liver Tissue ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Poly Vinyl Alcohol ◽  
Average Particle ◽  
Ag Nps

Liver regeneration is a highly organized tissue regrowth process and is the most important reaction of the injured liver. The present study endeavors towards the preparation and characterization of nanoporous Sodium Alginate (SA)/ Poly Vinyl Alcohol (PVA) composite, nanofibrous scaffolds coated with silver (Ag) nanoparticles for hepatocellular regeneration.  Chitosan based Silver nanoparticles possess high antibacterial activity has been preferred in the scaffold preparation to improve the antibacterial properties. The structural characterization of Ag Nanoparticles revealed the amorphous nature with an average particle size of 300 nm. Nanofibres (Scaffolds) were prepared by electrospinning SA/PVA solution at a voltage of 18-25 kV and Ag NPs were coated on it for antibacterial activity. Invitro studies denoted the growth of nitro compounds, amides and collagen which are the major constituents of liver tissue.

scholarly journals Antimicrobial efficacy of silver, zinc oxide, and titanium dioxide nanoparticles incorporated in orthodontic bonding agent

2019 ◽  
Vol 31 (3) ◽  
pp. 10-16 ◽  
Author(s):  
Sarah Y Hailan ◽  
Mustafa M. Al-Khatieeb
Keyword(s):  
Antimicrobial Agents ◽  
Titanium Dioxide Nanoparticles ◽  
Antibacterial Properties ◽  
Mutans Streptococci ◽  
Bonding Agent ◽  
Ag Nps ◽  
Zno Nps ◽  
White Spots ◽  
Orthodontic Bonding ◽  
Group 10

Background: One of the most important complications of fixed orthodontic treatment is formation of white spots, which are initial carious lesions. Addition of antimicrobial agents into orthodontic adhesive material might be wise solution for prevention of white spots formation. The aim of this study was to evaluate the antibacterial properties of orthodontic adhesive primer against S. Mutans after adding the three different types of nanoparticles (Ag, ZnO, or TiO2). Materials and methods: Discs were prepared using empty insulin syringe approximately 2 mm×2 mm rounded in shape specimens (40 discs) were divided into four groups (ten discs for each group): The first group was the control (made from primer only), the second group (10 discs made from primer and incorporation of Ag NPs), the third group (10 discs made from primer and incorporation of ZnO NPs), and the fourth group (10 discs made from primer and incorporation of TiO2 NPs). The antibacterial properties of orthodontic bonding agent after incorporation of (Ag, ZnO, or TiO2) nanoparticles were evaluated by disc diffusion test and viable count of mutans streptococci. Results: The results of this study showed that there were high significant differences between the all groups using ANOVA F-test, and the colony forming unit were 99×10³, 39.6×10³, 19.4×10³, 6.6×10³ CFU/mL respectively. Conclusion: The incorporation of these nanoparticles (Ag, ZnO, or TiO2) into Transbond ™ XT adhesive primer helps to enhance the antibacterial properties of primer against the S. mutans.

scholarly journals Antibacterial activity of zinc oxide with silver nanoparticles

2018 ◽  
Vol 7 (2) ◽  
pp. 98-107
Author(s):  
Fouzia Bourfaa ◽  
Abderhamane Boutelala ◽  
Mohamed Salah Aida ◽  
Nadir Attaf ◽  
Fateh Merouane
Keyword(s):  
Silver Nanoparticles ◽  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Ag Nanoparticles ◽  
Sol Gel ◽  
Zno Films ◽  
Ag Nps ◽  
Health Related ◽  
Uv Visible ◽  
Absorbance Peak

Nanoscale materials and their unique properties make them attractive for research and health -related applications. Silver nanoparticles were synthesised and incorporated in zinc oxide (ZnO) thin films on glass and tissue, in order to study their effect on antibacterial activity. ZnO films with various contents of Ag nanoparticles were prepared by the sol –gel method. Xray diffraction revealed the polycrystalline structure of the films, scanning electron microscopy exhibited their dense and continuous structure and UV-visible spectroscopy for measurement and transmittance was at more than 87%. The absorbance peak of Ag NPS was centred at 351 nm. The diameter of Ag NPS was analysed by Zeta Sizer and the colloids ranged from 2 to 110 nm. The results indicate that ZnO with Ag NPs on tissue can resist the growth of this kind of bacteria, with the zone of inhibition of the bacteria ATTC 700603 at between 8 and 18 mm .Keywords: Ag nanoparticles, zinc oxide, tissue, antibacterial activity.

scholarly journals Engineering of Antimicrobial Surfaces by Using Temporin Analogs to Tune the Biocidal/antiadhesive Effect

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 814 ◽  
Author(s):  
Pierre-Carl Oger ◽  
Christophe Piesse ◽  
Ali Ladram ◽  
Vincent Humblot
Keyword(s):  
Broad Spectrum ◽  
Bacterial Resistance ◽  
Antibacterial Properties ◽  
Covalent Immobilization ◽  
Resistant Bacteria ◽  
Gold Surfaces ◽  
Cationic Antimicrobial Peptide ◽  
Bacterial Membranes ◽  
Spectrum Activity ◽  
Broad Spectrum Activity

Proliferation of resistant bacteria on biomaterials is a major problem leading to nosocomial infections. Due to their broad-spectrum activity and their ability to disrupt bacterial membranes through a rapid membranolytic mechanism, antimicrobial peptides (AMPs) are less susceptible to the development of bacterial resistance and therefore represent good candidates for surface coating strategies to prevent biofilm formation. In this study, we report on the covalent immobilization of temporin-SHa, a small hydrophobic and low cationic antimicrobial peptide exhibiting broad-spectrum activity, and (SHa) analogs on modified gold surfaces. Several analogs derived from SHa with either a carboxamidated ([K3]SHa, d-[K3]SHa) or a carboxylated C-terminus ([K3]SHa-COOH) were used to achieve peptide grafting on gold surfaces modified by a thiolated self-assembled monolayer (SAM). Surface functionalization was characterized by polarization modulation infrared reflection absorption spectroscopy (PM-RAIRS) and X-ray photoemission spectroscopy (XPS). The antibacterial properties of the temporin-functionalized surfaces were tested against the Gram-positive Listeria ivanovii. Direct visualization of the peptide effects on the bacterial membrane was investigated by scanning electron microscopy equipped with a field emission gun (SEM-FEG). All active temporin analogs were successfully grafted and display significant antibacterial activity (from 80 to 90% killing efficiency) in addition to a 2-fold decrease of bacterial adhesion when all d-SHa analogs were used.

PREPARATION OF ANTIBACTERIAL SILVER-CONTAINING SILICA NANOCOMPOSITE

2008 ◽  
Vol 15 (01n02) ◽  
pp. 117-122 ◽  
Author(s):  
YOUNG HWAN KIM ◽  
CHANG WOO KIM ◽  
HYUN GIL CHA ◽  
BYOUNG KEE JO ◽  
GI WOONG AHN ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Ag Nanoparticles ◽  
Antibacterial Properties ◽  
Hybrid Structure ◽  
Ag Nanoparticle ◽  
Nanoparticle Deposition ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Ag nanoparticle deposition on the surface of spherical SiO 2 nanoparticles was studied to achieve hybrid structure of Ag – SiO 2 nanocomposite. SiO 2 nanoparticles were served as seeds for continuous Ag metal deposition. The ratio of elements and morphology was studied with scanning electron microscope energy dispersive X-ray and transmission electron microscope. The antibacterial properties of Ag – SiO 2 nanocomposite were examined. The homogeneously formed Ag nanoparticles on the surface of SiO 2 nanoparticles without aggregation of Ag nanoparticles showed excellent antibacterial abilities.

scholarly journals Exploration of Culturable Marine Streptomyces from Southern Coastal Region of India with Antibacterial Properties

2021 ◽  
pp. 323-334
Author(s):  
Deepika Thandayuthapani ◽  
Nivetha Chinnappa ◽  
Arjunan Annavi ◽  
Muthusevam Manickam
Keyword(s):  
Broad Spectrum ◽  
Tamil Nadu ◽  
Antibacterial Properties ◽  
Coastal Region ◽  
Salmonella Typhi ◽  
Industrial Applications ◽  
Shigella Dysenteriae ◽  
Abiotic Stress Condition ◽  
Spectrum Activity ◽  
Broad Spectrum Activity

The Southern coastal region is a geographically positioned area with rich source of microbial diversity, of which producing broad spectrum of bioactive compounds. The marine sediments are collected from the various sites of south east coastal region of Tamil nadu and are processed. Out of 78 isolates, the 4 strains of actinomycetes are showing broad spectrum activity against Klebsiella pneumoniae (MTCC 1687), Proteus vulgaris (MTCC 3160), Salmonella typhi (MTCC 3231), Shigella dysenteriae (MTCC 3642) and Vibrio cholerae (MTCC 3906). The bioactive isolates are proceeded for further morphological features like growth pattern and mycelial coloration, biochemical and polyphasic taxonomical characterisation were documented.  Isolates are investigated for abiotic stress condition to study the growth rate of isolates. Finally, the 16S rRNA molecular identification and phylogenetic analysis of the isolates were explored. Further, bioactive isolates may be potential source for discovery of molecules with industrial applications.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

Antimicrobial Peptides: A Promising Avenue for Human Healthcare

2020 ◽  
Vol 21 (2) ◽  
pp. 90-96 ◽  
Author(s):  
Girish M. Bhopale
Keyword(s):  
Antimicrobial Peptides ◽  
Antimicrobial Peptide ◽  
Antimicrobial Agents ◽  
Current Status ◽  
Antimicrobial Drugs ◽  
Spectrum Activity ◽  
Low Levels ◽  
Human Healthcare ◽  
Broad Spectrum Activity ◽  
Promising Avenue

Antimicrobial drugs resistant microbes have been observed worldwide and therefore alternative development of antimicrobial peptides has gained interest in human healthcare. Enormous progress has been made in the development of antimicrobial peptide during the last decade due to major advantages of AMPs such as broad-spectrum activity and low levels of induced resistance over the current antimicrobial agents. This review briefly provides various categories of AMP, their physicochemical properties and mechanism of action which governs their penetration into microbial cell. Further, the recent information on current status of antimicrobial peptide development, their applications and perspective in human healthcare are also described.

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