Mesenchymal Stem Cells from Bichat's Fat Pad:In VitroComparison with Adipose-Derived Stem Cells from Subcutaneous Tissue

Cancer is a leading cause of mortality and a major public health problem worldwide. For biological therapy against cancer, we previously developed a unique immunotherapeutic platform by combining mesenchymal stem cells with an antigen-specific protein vaccine. However, this system possesses a few limitations, such as improperly immortalized mesenchymal stem cells (MSCs) along with transfected oncogenic antigens in them. To overcome the limitations of this platform for future clinical application, we freshly prepared primary adipose-derived stem cells (ADSCs) and modified the E7’ antigen (E7’) as a non-oncogenic protein. Either subcutaneously co-inoculated with cancer cells or systemically administered after tumor growth, ADSC labeled with enhanced green fluorescent protein (eGFP) and combined with modified E7’ (ADSC-E7’-eGFP) cells showed significant antitumor activity when combined with the protein vaccine in both colon and lung cancer in mice. Specifically, this combined therapy inhibited tumor through inducing cell apoptosis. The significantly reduced endothelial cell markers, CD31 and vascular endothelial growth factor (VEGF), indicated strongly inhibited tumor angiogenesis. The activated immune system was demonstrated through the response of CD4+ T and natural killer (NK) cells, and a notable antitumor activity might be contributed by CD8+ T cells. Conclusively, these evidences imply that this promising immunotherapeutic platform might be a potential candidate for the future clinical application against cancer.

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Adipose-Derived Stem Cells in Crohn's Rectovaginal Fistula

Case Reports in Medicine ◽

10.1155/2010/961758 ◽

2010 ◽

Vol 2010 ◽

pp. 1-3 ◽

Cited By ~ 52

Author(s):

D. García-Olmo ◽

D. Herreros ◽

P. De-La-Quintana ◽

H. Guadalajara ◽

J. Trébol ◽

...

Keyword(s):

Adverse Effect ◽

Stem Cells ◽

Clinical Trials ◽

Mesenchymal Stem Cells ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Adipose Derived Stem Cells ◽

Vaginal Fistula ◽

History Of ◽

First Time

Therapeutic options for recto-vaginal fistula in the setting of Crohn's disease are limited and many data are available in the literature. The manuscript describes the history of a patient who has been the pioneer of our Clinical Trials in treating this disease in fistulizing Crohn's disease environment. We believe it is the first time that a patient with this disease has been treated by adipose-derived stem cells in allogeneic form. The conclusion of our study with Mary is that the use of mesenchymal stem cells derived from adipose tissue is secure, either in autologous or allogeneic form. Furthermore, we have proved that if we use multi-dose and multiple applications on a patient, it does not produce any adverse effect, which confirms us the safety of using these cells in patients at least in the fistulizing Crohn's disease environment.

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Recent Advance in Source, Property, Differentiation, and Applications of Infrapatellar Fat Pad Adipose-Derived Stem Cells

Stem Cells International ◽

10.1155/2020/2560174 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14 ◽

Cited By ~ 2

Author(s):

Yu-chen Zhong ◽

Shi-chun Wang ◽

Yin-he Han ◽

Yu Wen

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Regenerative Medicine ◽

3D Culture ◽

Infrapatellar Fat Pad ◽

Adipose Derived Stem Cells ◽

Fat Pad ◽

Stem Cell Source ◽

Marrow Mesenchymal Stem Cells ◽

And Function

Infrapatellar fat pad (IPFP) can be easily obtained during knee surgery, which avoids the damage to patients for obtaining IPFP. Infrapatellar fat pad adipose-derived stem cells (IPFP-ASCs) are also called infrapatellar fat pad mesenchymal stem cells (IPFP-MSCs) because the morphology of IPFP-ASCs is similar to that of bone marrow mesenchymal stem cells (BM-MSCs). IPFP-ASCs are attracting more and more attention due to their characteristics suitable to regenerative medicine such as strong proliferation and differentiation, anti-inflammation, antiaging, secreting cytokines, multipotential capacity, and 3D culture. IPFP-ASCs can repair articular cartilage and relieve the pain caused by osteoarthritis, so most of IPFP-related review articles focus on osteoarthritis. This article reviews the anatomy and function of IPFP, as well as the discovery, amplification, multipotential capacity, and application of IPFP-ASCs in order to explain why IPFP-ASC is a superior stem cell source in regenerative medicine.

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Are Adipose-Derived Stem Cells from Liver Falciform Ligaments Another Possible Source of Mesenchymal Stem Cells?

Cell Transplantation ◽

10.3727/096368916x693833 ◽

2017 ◽

Vol 26 (5) ◽

pp. 855-866 ◽

Cited By ~ 4

Author(s):

Sang Woo Lee ◽

Jae Uk Chong ◽

Seon Ok Min ◽

Seon Young Bak ◽

Kyung Sik Kim

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Liver Disease ◽

Subcutaneous Adipose Tissue ◽

Falciform Ligament ◽

Pcr Analysis ◽

Marker Genes ◽

Adipose Derived Stem Cells ◽

Subcutaneous Adipose

Falciform ligaments in the liver are surrounded by adipose tissue. We investigated the capability of adipose-derived stem cells from human liver falciform ligaments (hLF-ADSCs) to differentiate into hepatic-type cells and confirmed the functional capacity of the cells. Mesenchymal stem cells (MSCs) were isolated from the liver falciform ligament and abdominal subcutaneous adipose tissue in patients undergoing partial hepatectomy for liver disease. Cells were cultivated in MSC culture medium. Properties of MSCs were confirmed by flow cytometry, RT-PCR analysis, immunocytochemistry assays, and multilineage differentiation. Hepatic induction was performed using a three-step differentiation protocol with various growth factors. Morphology, capacity for expansion, and characteristics were similar between hLF-ADSCs and adipose-derived stem cells from human abdominal subcutaneous adipose tissue (hAS-ADSCs). However, hematopoietic– and mesenchymal–epithelial transition (MET)-related surface markers (CD133, CD34, CD45, and E-cadherin) had a higher expression in hLF-ADSCs. The hepatic induction marker genes had a higher expression in hLF-ADSCs on days 7 and 10 after the hepatic induction. Albumin secretion was similar between hLF-ADSCs and hAS-ADSCs at 20 days after the hepatic induction. The hLF-ADSCs had a different pattern of surface marker expression relative to hAS-ADSCs. However, proliferation, multilineage capacity, and hepatic induction were similar between the cell types. Accordingly, it may be a useful source of MSCs for patients with liver disease.

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Oral Cavity as a Source of Mesenchymal Stem Cells Useful for Regenerative Medicine in Dentistry

Biomedicines ◽

10.3390/biomedicines9091085 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1085

Author(s):

Ilaria Roato ◽

Giorgia Chinigò ◽

Tullio Genova ◽

Luca Munaron ◽

Federico Mussano

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Oral Cavity ◽

Therapeutic Strategy ◽

Adipose Derived Stem Cells ◽

Multilineage Differentiation ◽

Therapeutic Approaches ◽

Cytokines And Chemokines ◽

Specific Tissue ◽

Different Sources

The use of mesenchymal stem cells (MSCs) for regenerative purposes has become common in a large variety of diseases. In the dental and maxillofacial field, there are emerging clinical needs that could benefit from MSC-based therapeutic approaches. Even though MSCs can be isolated from different tissues, such as bone marrow, adipose tissue, etc., and are known for their multilineage differentiation, their different anatomical origin can affect the capability to differentiate into a specific tissue. For instance, MSCs isolated from the oral cavity might be more effective than adipose-derived stem cells (ASCs) for the treatment of dental defects. Indeed, in the oral cavity, there are different sources of MSCs that have been individually proposed as promising candidates for tissue engineering protocols. The therapeutic strategy based on MSCs can be direct, by using cells as components of the tissue to be regenerated, or indirect, aimed at delivering local growth factors, cytokines, and chemokines produced by the MSCs. Here, the authors outline the major sources of mesenchymal stem cells attainable from the oral cavity and discuss their possible usage in some of the most compelling therapeutic frontiers, such as periodontal disease and dental pulp regeneration.

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Metabolic Analysis of Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells Using NMR-Based Metabolomics.

Blood ◽

10.1182/blood.v108.11.4244.4244 ◽

2006 ◽

Vol 108 (11) ◽

pp. 4244-4244

Author(s):

Rei Ogawa ◽

Juri Fujimura ◽

Keiko Hirakawa ◽

Kyoko Uekusa ◽

Kohtaro Yuta ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Computer Software ◽

Principal Component ◽

Adipose Derived Stem Cells ◽

Detection Analysis ◽

Marrow Mesenchymal Stem Cells ◽

Metabolic Activities ◽

And Control

Abstract Background: Nuclear magnetic resonance (NMR) -based metabolomics has been suggested to be useful for exhaustive analysis of metabolic pathways of cells or tissues. We report our study on bone marrow mesenchymal stem cells (BSCs) and adipose-derived stem cells (ASCs) using NMR-based metabolomics. Materials and Methods: Five-week-old Wister rats were used for this study. BSCs were harvested from the femur, ASCs from inguinal fat pads, and control fibroblasts from the abdominal dermis. The cells were cultured in DMEM with 10% fetal bovine serum and harvested after two passages of the subculture. Then, the cells were subjected to freezing in liquid nitrogen and crushed to extract the aqueous metabolites. 1H-NMR spectra was measured and analyzed by a computer software (Alice2 for metabolome™ and ADMEWORKS/ModelbuilderTM). Results: BSCs, ASCs and fibroblasts were clearly separated into three groups on a principal component analysis (PCA) plot. Conclusion: ASCs, BSCs and fibroblasts were considered to have different metabolic activities, and NMR-based metabolomics will henceforth be useful for the detection, analysis, and characterization of various kinds of stem cells.

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3,6-Bis(1-methyl-4-vinylpyridinium)-carbazole diiodide as a marker for tracking living neural stem/precursor cells

Journal of Materials Chemistry B ◽

10.1039/c4tb01903b ◽

2015 ◽

Vol 3 (10) ◽

pp. 2067-2074 ◽

Cited By ~ 1

Author(s):

Yi-Chen Li ◽

Jyh-Horng Wang ◽

Li-Kai Tsai ◽

Yun-An Chen ◽

Ta-Chau Chang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Precursor Cells ◽

Adipose Derived Stem Cells ◽

Marrow Mesenchymal Stem Cells

A novel compound, 3,6-bis(1-methyl-4-vinylpyridinium)-carbazole diiodide, is used as a marker for distinguishing living neural stem/precursor cells (NSPCs) from adipose-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMMSCs).

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Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ

Stem Cells International ◽

10.1155/2016/1267480 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 8

Author(s):

Kaisaier Aji ◽

Munila Maimaijiang ◽

Abudusaimi Aimaiti ◽

Mulati Rexiati ◽

Baihetiya Azhati ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Smooth Muscle ◽

Protein Kinase ◽

Adipose Derived Stem Cells ◽

Dependent Protein Kinase ◽

Protein Levels ◽

Adult Mesenchymal Stem Cells ◽

Dependent Protein ◽

Muscle Contractile

The multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear. In the present study, we detectedγisoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days. The results showed that CaMKIIγincreased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis. The siRNA-mediated knockdown of CaMKIIγdecreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγoverexpression increases the transcriptional and protein levels of smooth muscle contractile markers. These results suggested thatγisoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs.

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Histological evaluations of apatite-fiber scaffold cultured with mesenchymal stem cells by implantation at rat subcutaneous tissue

Bio-Medical Materials and Engineering ◽

10.3233/bme-171656 ◽

2017 ◽

Vol 28 (1) ◽

pp. 57-64 ◽

Cited By ~ 2

Author(s):

Kitaru Suzuki ◽

Kohei Nagata ◽

Tomohiro Yokota ◽

Michiyo Honda ◽

Mamoru Aizawa

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Subcutaneous Tissue

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Evaluation of the Biocompatibility and Osteoconduction of the Carbon Nanotube, Chitosan and Hydroxyapatite Nanocomposite with or without Mesenchymal Stem Cells as a Scaffold for Bone Regeneration in Rats

Osteology ◽

10.3390/osteology1030013 ◽

2021 ◽

Vol 1 (3) ◽

pp. 118-131

Author(s):

Geissiane M. Marcondes ◽

Nicole F. Paretsis ◽

Joice Fülber ◽

Pedro Enriqu Navas-Suárez ◽

Claudia M. C. Mori ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Carbon Nanotube ◽

Bone Regeneration ◽

Subcutaneous Tissue ◽

Bone Substitutes ◽

Bone Defects ◽

Regenerative Process ◽

Control Group ◽

Marrow Mesenchymal Stem Cells

Background: Bone substitutes have been developed to assist bone regeneration in orthopedic surgeries. Mesenchymal stem cells can be added to these biomaterials to enhance bone regeneration. This study aimed to evaluate the biocompatibility and osteoconduction of a carbon nanotube, chitosan, and hydroxyapatite nanocomposite (CNCHN) that had either been enriched or not enriched with sheep bone marrow mesenchymal stem cells (BM-MSCs) in rats. Methods: A total of sixty rats were divided into groups, and an implant with or without BM-MSCs was performed subcutaneously in 20 animals (euthanized after 7 and 30 days), comparing them to 10 control animals, and in the calvaria of 20 animals (euthanized after 20 and 60 days), comparing to with 10 control animals. Subcutaneous and calvaria histologies were performed after euthanasia. Results: The subcutaneous tissue showed that CNCHN did not prompt an exacerbated inflammatory response or signs of necrosis. The histomorphological analysis by the calvaria score of the rats showed that the control group had lower scores at 20 and 60 days for bone neoformation, relative to the CNCHN groups, which showed no significant statistical differences, suggesting that the nanocomposite assisted in the regenerative process of defects in the calvaria, but with no repair potentiation when using BM-MSCs. Conclusion: CNCHN has biocompatibility and osteoconductive potential, showing promising results in bone defects.

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