Effect of Morinda citrifolia and Annona muricata on Erhlich Tumor Cells in Swiss Albino Mice and In Vitro Fibroblast Cells

2019 ◽  
Vol 22 (1) ◽  
pp. 46-51
Author(s):  
Cleber da Silveira Torres ◽  
Francisnaira da Silva Santos ◽  
Elen Landgraf Guiguer ◽  
Adriano Cressoni de Araújo ◽  
Sandra Maria Barbalho ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Morinda Citrifolia ◽  
Fibroblast Cells ◽  
Annona Muricata ◽  
Swiss Albino Mice ◽  
Albino Mice

scholarly journals Evaluation of in vitro and in vivo toxicity of pristine molybdenum disulphide nanosheets in Swiss albino mice

2021 ◽  
Author(s):  
Preeti S Saxena ◽  
Umakant Yadav ◽  
Himanshu Mishra ◽  
Vimal Singh ◽  
Anchal Srivastava
Keyword(s):  
Biomedical Applications ◽  
Culture Media ◽  
Lethal Dose ◽  
Intraperitoneal Administration ◽  
Significant Alteration ◽  
Swiss Albino Mice ◽  
Study Results ◽  
Serum Biochemical ◽  
Albino Mice

The Molybdenum disulfide nanosheets (MoS2-NSs) thin films has received increasing attention recently due to their versatile multi functionality including catalytic properties, photoluminescence and flexibility, which suggests their future, uses for biomedical applications. However, there are no studies in detail related with biocompatibility of MoS2 thin sheets. Here, weevaluated the dose-dependent effects of MoS2-NSs on cell viability (MTT assay) and release of lactate dehydrogenase (LDH) into culture media using MG-63 cells, as well as haemolysis, hematological, serum biochemical, antioxidants and histopathological parameters in Swiss albino mice. The MoS2-NSs was synthesized via facile hydrothermal method and characterized using XRD, Raman, SEM, TEM and HRTEM. The in vitro study results suggest that at lower concentration MoS2-NSs does not causes any toxicity. The lethal dose (LD50) was evaluated by intraperitoneal administration with different concentrations and estimated as ~1.0 mg kg-1. The higher dose (1.5 mg kg-1) of MoS2-NSs showed significant alteration in hematological markers and serum biochemical enzymes, as compared to control. Lipid peroxidation also shows significant alteration with respect to the control. Histopathological, hematological and biochemical examination, revealed no remarkable changes at lower concentration (less than 1.0 mg kg-1), however, higher concentration (1.5 mg kg-1) causes significant histopathological, antioxidants and biochemical alterations in tissues and serum, respectively. The results suggest that the lower concentration of MoS2-NSs can be used in future biomedical applications.

Extract of Tagetes Erecta Could Be Used as a Potential Drug Candidate Against Cancer: A Study on the Anticancer Efficacy of Medicinal Plants Involving in Vitro and in Vivo Approach

2021 ◽  
Author(s):  
Dharmeswar Barhoi ◽  
Puja Upadhaya ◽  
Sweety Nath Barbhuiya ◽  
Anirudha Giri ◽  
Sarbani Giri
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Flow Cytometric Analysis ◽  
Cancer Cell Lines ◽  
Tagetes Erecta ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Eac Cells

Abstract Globally, the burden of cancer is increasing consistently. Modern cancer therapies include lots of toxicity in the non-targeted organs reducing the life expectancy of the patients. Therefore, the development of safer alternative medicines with less toxicity and high efficacy is of immense importance. The present study was designed to evaluate the anticancer activity of a medicinal plant, “Tagetes erecta” (TE), in established cancer cell lines in vitro and in animal models in vivo. GC-MS analysis was performed that revealed hexadecanoic acid, Linolenic acid, Quinic acid, 2,3- dihydrobenzofuran (Coumaran), and β-stigmasterol as major bioactive compounds in TE leaves. Aqueous extract of Tagetes erecta (AETE) treatment potentially reduced the tumor weight (TW) and tumor volume (TV) and increased the life span in EAC-induced tumor-bearing Swiss albino mice. Side effect analysis confirmed the lack of toxicity of AETE to non-targeted organs in normal Swiss albino mice. Studies in cancer cell lines indicated dose and time-dependent cytotoxicity in Human laryngeal carcinoma (HEp-2) and Ehrlich ascites carcinoma (EAC) cells. Flow cytometric analysis established significant induction of apoptosis in EAC cells without arresting the cell cycle. In addition, AETE treatment led to a significant increase in cells with depolarised mitochondrial membrane potential. The present study indicated that AETE potentially inhibits tumor progression without disturbing normal body physiology. Thus, we conclude that AETE can be used as a potential therapeutic agent against cancer.

scholarly journals Safety Evaluations of Single Dose of the Olive Secoiridoid S-(−)-Oleocanthal in Swiss Albino Mice

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 314 ◽  
Author(s):  
Abu Bakar Siddique ◽  
Judy Ann King ◽  
Sharon A. Meyer ◽  
Khaldoun Abdelwahed ◽  
Belnaser Busnena ◽  
...  
Keyword(s):  
Olive Oil ◽  
Biological Effects ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Research Attention ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
A Minor

Epidemiological and clinical studies compellingly showed the ability of Mediterranean diet rich in extra-virgin olive oil (EVOO) to reduce multiple diseases such as cancer, cardiovascular diseases, and aging cognitive functions decline. The S-(−)-Oleocanthal (OC) is a minor phenolic secoiridoid exclusively found in extra-virgin olive oil (EVOO). OC recently gained notable research attention due to its excellent in vitro and in vivo biological effects against multiple cancers, inflammations, and Alzheimer’s disease. However, OC safety has not been comprehensively studied yet. This study reports for the first time the detailed safety of oral single OC dose in Swiss albino mice, applying the OECD 420 procedure. Male and female Swiss albino mice (n = 10) were orally treated with a single OC dose of either 10, 250, or 500 mg/kg bodyweight or equivalent volumes of distilled water. Mice fed a regular diet, and carefully observed for 14 days. Further, mice were then sacrificed, blood samples, and organs were collected and subjected to hematological, biochemical, and histological examinations. OC 10 mg/kg oral dose appears to be without adverse effects. Further, 250 mg/kg OC, p.o., is suggested as a possible upper dose for preclinical studies in the future.

scholarly journals Anticancer Potential of Aqueous Ethanol Seed Extract ofZiziphus mauritianaagainst Cancer Cell Lines and Ehrlich Ascites Carcinoma

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Tulika Mishra ◽  
Madhu Khullar ◽  
Aruna Bhatia
Keyword(s):  
Cell Lines ◽  
Ehrlich Ascites Carcinoma ◽  
Seed Extract ◽  
Ziziphus Mauritiana ◽  
Dependent Manner ◽  
Swiss Albino Mice ◽  
Ehrlich Ascites ◽  
Albino Mice

Ziziphus mauritiana(Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract ofZiziphus mauritiana in vitroagainst different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well asin vivoagainst Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 μg/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potentialin vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100–800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.

scholarly journals Evaluation of anti-anxiety activity of Mucuna pruriens

2019 ◽  
Vol 9 (4-A) ◽  
pp. 104-107
Author(s):  
Shobhit Singh ◽  
Pushpraj Gupta ◽  
Rishikesh Gupta
Keyword(s):  
Elevated Plus Maze ◽  
Standard Dose ◽  
The Body ◽  
Mucuna Pruriens ◽  
Control Group ◽  
Velvet Bean ◽  
Swiss Albino Mice ◽  
Plus Maze ◽  
Albino Mice

Mucuna pruriens, also known as Velvet Bean, Mucuna pruriens has been used for centuries by Ayurvedic herbalists for overall wellness. Mucuna pruriens provides support for brain function, muscle health and libido. Mucuna pruriens has also been shown to have diuretic effects. It increases tissue resiliency and improves coordination. Mucuna can also increase testosterone levels, which in turn can lead to increased muscle mass and strength. It also supports the nervous and reproductive systems in the body. anti-oxidant activity of M. pruriens has been also demonstrated in vitro by its ability to scavenge DPPH radicals and reactive oxygen species. This is an excellent natural source of L-dopa and 5-hydroxy tryptophan (5-HT) Present study was designed to evaluate the anti-anxiety activity of Mucuna pruriens extract in Swiss albino mice. Three doses of Mucuna pruriens (100, 200,400 mg/kg, p.o.) and standard dose of Buspirone (5 mg/kg, i.p.) were used for evaluation of the anti-anxiety activity. The elevated plus maze (EPM) was used to take as a measure of antianxiety effect. Mucuna pruriens at the doses of 200 mg/kg and 400 mg/kg significantly reduced the time spent and no. of entries in closed arm, increased the time spent and entries into open arm in elevated plus maze (p<0.05) as compared to control group. These results indicate that MP may be possesses antianxiety property. Keywords:  Anxiety, Elevated plus maze, Mucuna pruriens, Buspirone, Swiss Albino Mice.

scholarly journals Análise in vitro da atividade antifúngica de extratos vegetais frente a leveduras pertencentes à espécie Candida albicans

2021 ◽  
Vol 10 (10) ◽  
pp. e440101019075
Author(s):  
Lara Vitória de Araújo Costa Pereira ◽  
Luciane Costa Silva ◽  
Marcela Coelho de Sá ◽  
Mariana de Carvalho Moreira ◽  
Gabriel Lima Maia Soares do Nascimento ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Apis Mellifera ◽  
Morus Alba ◽  
Morinda Citrifolia ◽  
Annona Muricata ◽  
Caryocar Brasiliense

A candidíase é uma das afecções mais comuns, estimando-se que a grande parte das mulheres apresentará, ao longo da vida, pelo menos um episódio da infecção. E com isso a importância de estudos sobre outros métodos alternativos para tratamento baseados em extratos vegetais, os quais apresentam potencial ecológico para substituir o emprego de produtos sintéticos. Assim, a pesquisa teve como objetivo analisar a atividade antifúngica de extratos brutos dos vegetais Morinda citrifolia (None), Caryocar brasiliense (Pequi), Annona muricata (Graviola), Morus alba L. (Amora) e Apis mellífera L. (Própolis) frente a levedura Candida albicans. Os vegetais foram coletados no município de Teresina-PI e secos a temperatura ambiente sob a sombra, com exposição diária de 4 h; moídos em moinho de facas; os extratos foram obtidos em metanol e os testes de suscetibilidade foram realizados em triplicata nos meios de cultura ágar-ágar e ágar Muller-Hinton, pela técnica da difusão em ágar. Utilizou-se a cepa de Candida albicans ATCC-76485 nos testes. Dos cinco vegetais utilizados, os extratos de Pequi e de Própolis, apresentaram atividade antifúngica sobre a cepa de C. albicans, com o segundo extrato apresentando maior poder de ação sobre a levedura. Portanto, os extratos do Apis melífera L. e do Caryocar brasiliense apresentaram atividade anti C. albicans, enquanto os de Morus alba L., Morinda citifolia L. e Annona muricata L. não apresentaram.

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