Concentration of glucocorticoid receptor sites in normal human lymphocytes.

1986 ◽  
Vol 32 (1) ◽  
pp. 80-83 ◽  
Author(s):  
A E Steiner ◽  
J L Wittliff
Keyword(s):  
Glucocorticoid Receptor ◽  
Human Lymphocytes ◽  
Normal Subjects ◽  
Mean Value ◽  
Whole Cell ◽  
Receptor Sites ◽  
Human T Cells ◽  
Cell Assays ◽  
Normal Human ◽  
The Mean

Abstract We used a whole-cell glucocorticoid receptor assay to examine characteristics of the glucocorticoid receptor in the lymphocytes of normal human donors. We measured binding of [3H]dexamethasone to the lymphocytes of four different donors on several different occasions; the variation about the mean for the assays was +/- 15%. Whole-cell assays in 15 normal subjects showed a mean value of 6.18 fmol/10(6) cells or 3722 sites per cell, with a somewhat higher level in men (7.67 fmol/10(6) cells, or 4620 sites per cell) than women (4.48 fmol/10(6) cells, or 2698 sites per cell). We saw no correlation between donor age and receptor values, in either group. Assays in which we used [3H]prednisolone demonstrated similar binding properties as with [3H]dexamethasone. The mean glucocorticoid receptor value for normal human T-cells from three donors was 2.52 fmol/10(6) cells, or 1518 sites per cell.

Effect of 1,25-(OH)2D3 on jejunal absorption of magnesium in patients with chronic renal disease

1980 ◽  
Vol 238 (4) ◽  
pp. G349-G352 ◽  
Author(s):  
A. C. Schmulen ◽  
M. Lerman ◽  
C. Y. Pak ◽  
J. Zerwekh ◽  
S. Morawski ◽  
...  
Keyword(s):  
Vitamin D ◽  
Renal Disease ◽  
Chronic Renal Disease ◽  
Normal Subjects ◽  
Mean Value ◽  
Normal Serum ◽  
Perfusion Technique ◽  
Dihydroxyvitamin D3 ◽  
Jejunal Absorption ◽  
The Mean

These studies were performed to see if jejunal malabsorption of magnesium in patients with chronic renal disease was influenced by therapy with 1 alpha, 25-dihydroxyvitamin D3 [1,25-(OH)2D3; 2 microgram/day by mouth for 7 days]. This treatment restored normal serum concentrations of the vitamin D metabolite from 0.9 +/- 0.2 to 4.2 +/- 0.6 ng/dl. Jejunal absorption of magnesium, measured by a triple-lumen constant-perfusion technique, was enhanced in each of the seven patients by this therapy. The mean value rose from 0.04 +/- 0.02 to 0.13 +/- 0.02 mmol . 30 cm-1 . h-1. This last value is similar to the magnesium absorption rate in untreated normal subjects. These results demonstrate that magnesium absorption in the human jejunum is dependent on vitamin D, and they show that 1 alpha,25-dihydroxyvitamin D3 therapy in patients with chronic renal failure is associated with an enhanced jejunal absorption of magnesium.

Rate of uptake of carbon monoxide at different inspired concentrations in humans

1982 ◽  
Vol 52 (1) ◽  
pp. 109-113 ◽  
Author(s):  
H. A. Jones ◽  
J. C. Clark ◽  
E. E. Davies ◽  
R. E. Forster ◽  
J. M. Hughes
Keyword(s):  
Carbon Monoxide ◽  
Transport System ◽  
Human Lung ◽  
Normal Subjects ◽  
Facilitated Transport ◽  
Diffusing Capacity ◽  
Normal Human ◽  
The Mean ◽  
Co Concentrations

The rate of uptake of carbon monoxide (CO) in the lungs of normal subjects were measured at inspired concentrations of less than 1, 300, and 3,000 ppm (less than 0.0001–0.3%) using radioactive CO (11CO). In nine subjects the rate of uptake was monitored at the mouth during rebreathing. At inspired CO concentrations of approximately 1, 300, and 3,000 ppm and a mean alveolar O2 fraction of 0.15, the mean lung diffusing capacity was 25.8, 26.4, and 25.3 ml . min-1. Torr-1, respectively. In seven subjects the measurements were repeated after a period of O2 breathing, giving a mean alveolar O2 fraction of 0.78. The calculated membrane diffusing capacity was 31.9, 33.7, and 32.0 ml . min-1. Torr-1 at less than 1, 300, and 3,000 ppm inspired CO. We conclude that there is no difference in the rate of uptake of CO over the range of concentrations studied in these experiments. No evidence for the presence of a facilitated transport system for CO in the normal human lung was found.

Isotope Studies in Normal and Diseased Knee Joints: 99mTc Uptake Related to Clinical Assessment and to Synovial Perfusion Measured by the 133Xe Clearance Technique

1971 ◽  
Vol 40 (4) ◽  
pp. 327-336 ◽  
Author(s):  
W. C. Dick ◽  
S. D. Deodhar ◽  
Carol J. Provan ◽  
G. Nuki ◽  
W. W. Buchanan
Keyword(s):  
Rheumatoid Arthritis ◽  
Normal Subjects ◽  
Knee Joints ◽  
133Xe Clearance ◽  
Normal Limits ◽  
Human Volunteers ◽  
Normal Human ◽  
Clinical Indices ◽  
Isotope Studies ◽  
The Mean

1. Uptake of intravenously administered radioactive technetium (99mTc) was measured over the knee joints in normal human volunteers, in patients with osteoarthritis and in groups of synovectomized and unoperated patients with rheumatoid arthritis. The uptake was compared with clinical indices of inflammation (pain, tenderness swelling and stiffness), and the clearance rate of intra-articularly injected radioactive xenon (133Xe). The 99mTc uptakes were found to be unrelated to the isotope dose and the day-to-day reproducibility was acceptable. 2. The mean uptake of 99mTc was within normal limits in osteoarthritis. Both in synovectomized and in unoperated rheumatoid arthritis 99mTc uptake was significantly higher than in normal subjects. 3. Of the clinical indices studied significant correlation of 99mTc uptake was found with pain and swelling in all groups of patients studied. 4. Faster clearance of 133Xe in unoperated rheumatoid arthritis correlated well with the higher 99mTc uptakes. 5. The results confirm that 99mTc uptakes are raised in inflammatory arthritis but not in degenerative arthritis. The relation of 99mTc uptake to the clinical indices of inflammation and to the 133Xe clearance from the joint is discussed.

Free Water Clearance in Patients with Essential Hypertension

1973 ◽  
Vol 45 (4) ◽  
pp. 469-477
Author(s):  
E. J. Dorhout Mees ◽  
H. de Graaf
Keyword(s):  
Salt Intake ◽  
Free Water ◽  
Normal Subjects ◽  
Mean Value ◽  
Exponential Relationship ◽  
Free Water Clearance ◽  
Uncomplicated Hypertension ◽  
The Mean ◽  
Different Levels ◽  
Water Clearance

1. Free water clearance (CH2O) was measured in sixteen normal subjects and twenty-five patients with uncomplicated hypertension at different levels of salt intake. 2. In normal subjects CH2O and Na+ excretion were related thus: log Na+ excretion = 0·1685 + 0·1508 × CH2O. At a given value for Na+ excretion the standard deviation for CH2O was ±1·5 ml min−1. 3. The mean value for the ratio CH2O/creatinine clearance was higher in the hypertensive patients than in controls at all levels of Na+ excretion. 4. Most previous studies reporting decreased values for CH2O in hypertension can be explained by the exponential relationship between CH2O and Na+ excretion.

Catechol O-Methyltransferase in Red Blood Cells of Schizophrenic, Depressed, and Normal Human Subjects

1976 ◽  
Vol 128 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Helen L. White ◽  
Malcolm N. McLeod ◽  
Jonathan R. T. Davidson
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Normal Subjects ◽  
Enzyme Preparations ◽  
Human Red Blood Cells ◽  
Schizophrenic Patients ◽  
Normal Human ◽  
The Mean ◽  
Isoelectric Ph

SummaryCatechol O-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, S-adenosyl-L-methionine and 3,4-dihydroxybenzoic acid. The mean enzyme activity found in 24 normal subjects was 29.2 nmol/hr/ml RBC. The mean activity in blood of 33 female unipolar depressives was not significantly different from normal. However, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38.9 nmol/hr/ml RBC). Partially purified enzyme preparations from blood of normal and schizophrenic individuals were indistinguishable with respect to substrate specificities, isoelectric pH values, and ratios of the two O-methylated products. Therefore it is unlikely that any defect in O-methylation which may occur in schizophrenia can be attributed to a change in the intrinsic properties of erythrocyte catechol O-methyltransferase.

CD59 Deficient Granulocytes in High Risk Groups of Myelodysplastic Syndromes (MDS).

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4717-4717
Author(s):  
Georgia Kaiafa ◽  
Vasiliki Tsavdaridou ◽  
Athanasios Papadopoulos ◽  
Christos Savopoulos ◽  
Apostolos Hatzitolios ◽  
...  
Keyword(s):  
Normal Subjects ◽  
Risk Groups ◽  
Mean Value ◽  
Control Group ◽  
Refractory Anaemia ◽  
Mean Values ◽  
Significant Difference ◽  
The Mean ◽  
Group B ◽  
Pnh Clone

Abstract Background: Blood cells are deficient in membrane-bound glycosyl-phosphatidylinositol (GPI)-anchored proteins in paroxysmal nocturnal hemoglobinuria (PNH) because of an acquired disorder of the pluripotent stem cell. CD55 and CD59 antigens are the most common GPI-anchored proteins that are used for the diagnosis of the presence of PNH clone. The association of MDS with PNH is rather controversial. There are few published individual cases of PNH arrived from a previous MDS or MDS following PNH. Aim: Recent studies have demonstrated the existence of PNH clone in some MDS patients. These studies were performed mostly on erythrocytes and to the best of our knowledge they are only 4. Granulocytes appear to be more sensitive markers of PNH clone existence than erythrocytes and therefore we investigate the expression of PNH clone on granulocytes of patients with MDS. Material-Methods: A total number of 95 patients 25–80 yrs old with MDS {A:19 with refractory anaemia (RA), B:9 with refractory anaemia with ring sideroblasts (RARS), C:17 with refractory anaemia with excess blasts (RAEB), D: 12 with refractory anaemia with excess blasts in transformation (RAEB-t) and E: 19 with chronic myelomonocytic leukemia (CMLL)} and 19 healthy donors were included in our study. The presence of GPI-anchored proteins (CD55, CD59)-deficient granulocytes was examined by flow cytometry. For the detection of the PNH clone the commercial kit by Beckman Coulter (cellquant CD55/CD59) was used. Statistical analysis was made by ANOVA, while Robust test was performed because there was no homogeneity of variances by ANOVA. Results: Table 1 shows the percentages of CD55 and CD59 deficient granulocytes in the 5 groups of pts with MDS comparing with the control group. a) Although the mean values of CD55 deficient granulocytes in the groups RAEB, RAEB-t and CMLL were higher enough than those of the normal subjects, there were no statistically significant differences (p>0.10) in the comparison of the mean values of CD55 deficient granulocytes between each group of patients with the control group. b) On the contrary there was a very statistically significant difference in the comparison of the mean value of CD59 deficient granulocytes between the three last groups (C, D and E) of pts with the control group (p< 0.086, p< 0.001 and p<0.001 respectively). The percentages of CD55 and CD59 deficient granulocytes in the 5 groups of patients with MDS and in the control group CD55 CD55 CD59 CD59 Groups N Mean Value Std deviation Mean Value Std deviation A (RA) 19 2.89 2.79 2.91 2.85 B (RARS) 9 1.05 1.14 3.89 3.63 C (RAEB) 17 4.78 4.76 11.75 6.06 D (RAEB-t) 12 4.23 2.85 23.76 8.53 E (CMLL) 19 6.01 4.97 38.26 16.18 Control 19 3.67 2.89 3.67 2.89 Total 95 4.00 3.87 14.44 16.39 Conclusions: There are relatively large CD59 negative subpopulations (mean values 11.75–38.26%) of granulocytes in the last three groups (RAEB, RAEB-t, CMLL) of MDS patients. On the contrary the two low risk forms (RA, RARS) revealed no such populations. Non-expression of CD59 antigen on granulocytes is a more sensitive marker than that of CD55 antigen for the presence of a possible PNH clone in MDS patients.

Effect of Erythropoietin on Platelet Function and Glycoprotein Expression in Patients with MDS.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3993-3993
Author(s):  
Fotis F.I. Girtovitis ◽  
Dimitrios D. Bougiouklis ◽  
Michel M.P. Makris ◽  
Elefteria E. Pithara ◽  
Pantelis P.E. Makris
Keyword(s):  
Flow Cytometry ◽  
Platelet Function ◽  
Normal Subjects ◽  
Mean Value ◽  
Special Treatment ◽  
Recombinant Erythropoietin ◽  
Mean Values ◽  
Human Recombinant Erythropoietin ◽  
The Mean ◽  
The Difference

Abstract Aim: We decide to study the effect of erythropoietin on the platelet function and glycoproteins expression in patients (pts) with myelodysplastic syndrome (MDS). All patients suffered from primary MDS but none of them received any special treatment. From our study pts that suffered from diseases or they were receiving medication that affect the function of platelets were excluded. Furthermore pts with platelet count&lt;50,000/μl were excluded also because we were unable to check the aggregation of platelets. Material: 41 subjects were studied, 15 normal subject (10 men and 5 women mean age 66,6±14 years old)and 26 pts (17 men and 9 women with mean age70,9±7,4 years old) suffered from all types of MDS according to FAB criteria (9 with RA, 3 with RARS, 7 RAEB, 4 with CMML and 3 with RAEB-t). We divided them in 2 groups: 1st - 7 Pts receiving human recombinant erythropoietin (rEPO) with mean dose 30.000 iu subcutaneous weekly and 2nd - 19 without rEPO. Methods. 1- The platelet function was studied in Platelet Ionized Calcium Aggregometer (PICA) using Ristocetin, ADP, Collagen and Adrenalin as stimulators. 2- The expression of platelet glycoproteins (GPIb, IX, IIb, IIIa and P-selectin) was studied using the flow cytometry and special monoclonal antibodies. This way the percentage of glycoprotein expressed in platelet membrane and MFI were estimated. We performed the statistical analysis of our results using the t-test with common standard deviation.. Results: our results concerning the aggregation test and flow cytometry are presented in tables 1,2 and 3. From the study of our results we can see that while the decrease of aggregation between the patients under EPO and normal subjects is statistically non significant (p&lt;0.1)the decrease of the corresponding values between the pts that did not received EPO and normal subjects was statistically very significant for all stimulators (p &lt;0,001). pts under EPO show an important increase of platelet expressing GPIIb percentage grater than the expressed percentage of patients without EPO (60% vs. 46,1% correspondingly, p&lt;0.001). The difference of expressed MFI was not statistically significant. Conclusion: The findings of our study show us that erythropoietin improves the function of platelets in patients with MDS, probably through the increase of platelet percentage which express glycoproteins. Table 1 Comparison of the mean value of aggregation between the groups Table 2 Comparison of the mean values of the platelets expressing corresponding glycoproteins percentage between the groups Table 3 Comparison of the mean value of MFI between the groups

scholarly journals Significance of Blood Cadmium Concentrations in Patients with Renal Disorders or Essential Hypertension and the Normal Population

1978 ◽  
Vol 15 (1-6) ◽  
pp. 197-200 ◽  
Author(s):  
Roberta J. Ward ◽  
Monica Fisher ◽  
Marisol Tellez-Yudilevich
Keyword(s):  
Essential Hypertension ◽  
Whole Blood ◽  
Normal Population ◽  
Cadmium Concentration ◽  
Normal Subjects ◽  
Mean Value ◽  
Renal Disorders ◽  
Control Subjects ◽  
Blood Cadmium ◽  
The Mean

Summary Cadmium concentrations in whole blood have been determined in normal control subjects, in patients with untreated essential hypertension or with treated essential hypertension, and in those with acute or chronic renal disorders. High cadmium concentrations were not found in the patients with untreated essential hypertension. Most tobacco smokers were found to have high blood cadmium concentrations. When the mean cadmium concentrations from each group of patients were compared with those of the control subjects no significant differences were found. When the four groups were divided into smokers and non-smokers, however, a significantly higher mean value was found for the non-smoking renal patients in comparison with the non-smoking normal subjects. The marked increases in blood cadmium concentration in the patients with renal disorders may be attributed to impaired excretion.

Objective cyclodeviation measurement in normal subjects by means of Cyclocheck® application

2020 ◽  
pp. 112067212090531
Author(s):  
Justyna Simiera ◽  
Agata Joanna Ordon ◽  
Piotr Loba
Keyword(s):  
Normal Subjects ◽  
Mean Value ◽  
Angle Measurements ◽  
Corrected Visual Acuity ◽  
Normal Individuals ◽  
Muscle Balance ◽  
Study Population ◽  
The Mean ◽  
Fundus Photographs ◽  
The Right

Purpose: To evaluate the range of cyclodeviation in normal individuals by means of Cyclocheck® application recently designed by the authors and freely available at www.cyclocheck.com . Methods: Healthy subjects with normal muscle balance, best-corrected visual acuity of ⩾0.8, and stereopsis on Randot charts of ⩽100 s of arc were included in the study. Two separate digital fundus photographs were taken of each eye of every patient. The disk-foveal angle was calculated using the Cyclocheck® application. The average result of the disk-foveal angle measurements were considered for data analysis. Results: A total of 131 patients met inclusion criteria for the study population. The mean value of the disk-foveal angle in the whole study group (both right and left eye) was 6.39° ± 2.72° with 5.26° ± 2.56° (range from −0.4° to 12.55°) in the right eye and 7.52° ± 2.39° (range from 1.25° to 12.76°) in the left eye. The mean value of the disk-foveal angle of the left eye was greater by 2.26° than that of the right eye. Conclusion: Cyclocheck® software allows easy assessment of cyclodeviation. Normal individuals present with a positive value of the disk-foveal angle with a certain spread of the results. The analysis of obtained measurements revealed a significant asymmetry between both eyes with the left eye being more excyclodeviated in an otherwise orthotropic population, which remains a subject for further investigations.

An Assessment of Radioimmunoassay Procedures for Determination of Anti-Acetylcholine Receptor Antibodies in the Sera of Patients with Myasthenia Gravis

1981 ◽  
Vol 18 (3) ◽  
pp. 146-152 ◽  
Author(s):  
B Carter ◽  
R Harrison ◽  
G G Lunt ◽  
H Morris ◽  
T Savage-Marengo ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Myasthenia Gravis ◽  
Acetylcholine Receptor ◽  
Binding Sites ◽  
Mean Value ◽  
Normal Human ◽  
The Mean ◽  
Receptor Antibodies ◽  
Anti Acetylcholine

A reproducible radioimmunoassay procedure for the determination of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is described and examined in detail. The assay combines features of a number of methods previously outlined and allows repeat determinations of antibody titre in a given myasthenic serum sample with coefficient of variation 6%. The mean ± standard deviation for normal human serum anti-acetylcholine receptor antibodies was found by this procedure to be 0·024 ± 0·033 nmol/l α-bungarotoxin binding sites whereas the range for myasthenic patients was 0–139·14 nmol/l with a mean value of 7·55 nmol/l α-bungarotoxin binding sites.

Sign in / Sign up

Export Citation Format

Share Document