Time-resolved immunofluorometric assay and specimen storage conditions for measuring urinary gonadotropins

1994 ◽  
Vol 40 (5) ◽  
pp. 749-753 ◽  
Author(s):  
M Saketos ◽  
N Sharma ◽  
T Adel ◽  
M Raghuwanshi ◽  
N Santoro
Keyword(s):  
Follicle Stimulating Hormone ◽  
Storage Conditions ◽  
Simple Method ◽  
Time Resolved ◽  
Wide Range ◽  
Acetone Extracts ◽  
And Storage ◽  
Range Of Values ◽  
Urine Specimens ◽  
Serum And Urine

Abstract We optimized storage conditions and validated a sensitive immunofluorometric assay (IFMA) for urinary gonadotropins. Assay linearity and parallelism for luteinizing hormone (LH) and follicle-stimulating hormone (FSH) was observed to 0.04 IU/L. Urinary LH and FSH were unaffected by changes of osmolarity from 0.5 to 3.0 mOsm/kg, and from pH 4.5 to 10.5. Serum and urine measurements of the hormones correlated well over a wide range of values: for LH, R2 = 0.94, P < 0.01; for FSH, R2 = 0.71, P < 0.01 (n = 304). Preservation of urine with glycerol (70 mL/L) and storage at -20 degrees C yielded > 80% recovery of LH and FSH after 51 weeks; this was comparable with recovery for acetone extracts of urine. Untreated urine showed loss of activity by 4 weeks of storage. Preserving urine specimens with glycerol is a simple method of storage for longitudinal study and compares favorably with acetone extraction. IFMAs can measure urinary gonadotropins reproducibly over a wide range of pH and osmotic conditions.

scholarly journals WAVE FORCES ON SQUARE CAISSONS

1976 ◽  
Vol 1 (15) ◽  
pp. 132 ◽  
Author(s):  
G.R. Mogridge ◽  
W.W. Jamieson
Keyword(s):  
Experimental Data ◽  
Theoretical Method ◽  
Wave Forces ◽  
Simple Method ◽  
Method Of Calculation ◽  
Wave Flume ◽  
Wide Range ◽  
Wave Heights ◽  
And Storage ◽  
Water Depths

The forces and overturning moments exerted by waves on large vertical square-section caissons have been measured in the laboratory. Each model caisson extended from the bottom of a wave flume through the water surface and was oriented either with one side perpendicular to the direction of wave propagation or turned through an angle of forty-five degrees to this position. For a given orientation, each model was tested for a range of wave heights (up to the point of breaking) for various wave periods and water depths. A digital computer was used for the acquisition, processing, plotting and storage of the experimental data. In addition to the experimental work, an approximate theoretical method is presented which allows the wave loadings on a square caisson to be estimated by means of a simple desk calculation. The experimental data shows that this simple method of calculation is reasonably accurate over a wide range of wave conditions and caisson sizes.

Standardization of a Method To Determine the Efficacy of Sanitizers in Inactivating Human Pathogenic Microorganisms on Raw Fruits and Vegetables

2001 ◽  
Vol 64 (7) ◽  
pp. 1079-1084 ◽  
Author(s):  
LARRY R. BEUCHAT ◽  
JEFFREY M. FARBER ◽  
EDITH H. GARRETT ◽  
LINDA J. HARRIS ◽  
MICKEY E. PARISH ◽  
...  
Keyword(s):  
Standard Method ◽  
Fruits And Vegetables ◽  
Storage Conditions ◽  
Basic Method ◽  
Pathogenic Microorganisms ◽  
Wide Range ◽  
Survival And Growth ◽  
And Storage ◽  
Reference Strains ◽  
Basic Standard

The efficacy of sanitizers in killing human pathogenic microorganisms on a wide range of whole and fresh-cut fruits and vegetables has been studied extensively. Numerous challenge studies to determine the effects of storage conditions on survival and growth of pathogens on raw produce have also been reported. Results of these studies are often difficult to assess because of the lack of sufficient reporting of methods or, comparatively, because of variations in procedures for preparing and applying inocula to produce, conditions for treatment and storage, and procedures for enumerating pathogens. There is a need for a standard method to accurately determine the presence and populations of pathogenic microorganisms on produce. The adoption of standard, well-characterized reference strains would benefit a comparative assessment of a basic method among laboratories. A single protocol will not be suitable for all fruits and vegetables. Modifications of a basic method will be necessary to achieve maximum recovery of pathogens on various types of produce subjected to different sanitizer or storage treatments. This article discusses parameters that must be considered in the course of developing a basic standard method against which these modifications could be made.

Short-Term Stability of Urine Electrolytes: Effect of Time and Storage Conditions

2021 ◽  
pp. 1-3
Author(s):  
J.D. Adams ◽  
Miranda Badolato ◽  
Ethan Pierce ◽  
Abbie Cantrell ◽  
Zac Parker ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Short Term ◽  
Urine Sodium ◽  
Sodium Potassium ◽  
Significant Difference ◽  
Urine Electrolytes ◽  
And Storage ◽  
Storage Temperatures ◽  
Urine Specimens

The purpose of this investigation was to quantify the effects of storage temperature and duration on the assessment of urine electrolytes. Twenty-one separate human urine specimens were analyzed as baseline and with the remaining specimen separated into eight vials, two in each of the following four temperatures: 22, 7, −20, and −80 °C. Each specimen was analyzed for urine electrolytes (sodium, potassium, and chloride) after 24 and 48 hr. After 24 hr, no significant difference was detected from baseline in urine sodium, potassium, and chloride at all four storage temperatures (p > .05). Similarly, after 48 hr, urine sodium, potassium, and chloride were not significantly different from baseline in all four storage temperatures (p > .05). In conclusion, these data show that urine specimens analyzed for urine sodium, chloride, and potassium are stable up to 48 hr in temperatures ranging from deep freezing to room temperature.

scholarly journals Estimation of the true infection rate and infection fatality rate of coronavirus disease 2019 in each country

2020 ◽  
Author(s):  
Masahiro Sonoo ◽  
Takamichi Kanbayashi ◽  
Takayoshi Shimohata ◽  
Masahito Kobayashi ◽  
Masashi Idogawa ◽  
...  
Keyword(s):  
Infection Rate ◽  
Open Data ◽  
Fatality Rate ◽  
Simple Method ◽  
Viral Virulence ◽  
Wide Range ◽  
True Infection ◽  
Range Of Values

The True Infection Rate (TIR) in the whole population of each country and the Infection Fatality Rate (IFR) for coronavirus disease 2019 (COVID-19) are unknown. We devised a simple method to infer TIR and IFR based on the open data. The estimated TIR was compared with local antibody surveys. Estimated IFR took on a wide range of values up to 10%. The importance of the attenuation of the viral virulence is emphasized.

scholarly journals HPLC Analysis of Aflatoxin B1 Contamination of Local and Foreign Brands of Cigarette Tobacco Popularly Consumed in Niger State North-Central Nigeria

2020 ◽  
pp. 241-248
Author(s):  
Ndagi Mohammed Saba ◽  
Musa Achimugu Dickson ◽  
Ogbiko Cyril
Keyword(s):  
Aflatoxin B1 ◽  
Hplc Analysis ◽  
Health Hazard ◽  
Growth Conditions ◽  
Storage Conditions ◽  
Cigarette Tobacco ◽  
Wide Range ◽  
Processing And Storage ◽  
Foreign Brands ◽  
And Storage

Under favorable growth conditions, fungi produced aflatoxins. Aflatoxin B1 (AFB1) categorized as a carcinogen is the most toxic and subject of regulation in foods and feeds in many countries. AFB1 contamination has been reported in a wide range of products including cigarette tobacco due to improper processing and storage conditions. This research aim at determining the concentration load of AFB1 mycotoxin in brands of cigarette tobacco commonly consumed in Lapai and Minna metropolis of Niger State Nigeria. Thirty-six (36) brands of cigarette tobacco were purchased in open markets in Minna and Lapai towns of Niger state, Nigeria. Residual AFB1 was extracted from the sample using standard procedures. HPLC analysis was used for the mycotoxin quantification. The result showed that all cigarette samples produces AFB1 with a concentration of 302 µg/mL and 18 µg/mL as the highest and lowest AFB1 concentration respectively for the local – Nigeria brands compared to 166 µg/mL and 8 µg/mL AFB1 concentration observed for the foreign brands. The findings from this study provide sufficient levels of contamination to pose significant health hazard for the consumer of the tobacco. Careful processing and storage is therefore advocated to eliminate or reduce the mycotoxin contamination of the cigarette brands.

Reduction of carbon impurities in aluminium nitride from time-resolved chemical vapour deposition using trimethylaluminum

2020 ◽  
Author(s):  
Polla Rouf ◽  
Pitsiri Sukkaew ◽  
Lars Ojamäe ◽  
Henrik Pedersen
Keyword(s):  
Chemical Vapour Deposition ◽  
Vapour Deposition ◽  
Chemical Vapour ◽  
Aluminium Nitride ◽  
Methyl Groups ◽  
Time Resolved ◽  
Thermally Induced ◽  
Light Emitting ◽  
Carbon Impurities ◽  
Wide Range

<p>Aluminium nitride (AlN) is a semiconductor with a wide range of applications from light emitting diodes to high frequency transistors. Electronic grade AlN is routinely deposited at 1000 °C by chemical vapour deposition (CVD) using trimethylaluminium (TMA) and NH<sub>3</sub> while low temperature CVD routes to high quality AlN are scarce and suffer from high levels of carbon impurities in the film. We report on an ALD-like CVD approach with time-resolved precursor supply where thermally induced desorption of methyl groups from the AlN surface is enhanced by the addition of an extra pulse, H<sub>2</sub>, N<sub>2</sub> or Ar between the TMA and NH<sub>3</sub> pulses. The enhanced desorption allowed deposition of AlN films with carbon content of 1 at. % at 480 °C. Kinetic- and quantum chemical modelling suggest that the extra pulse between TMA and NH<sub>3</sub> prevents re-adsorption of desorbing methyl groups terminating the AlN surface after the TMA pulse. </p>

The content of decenoic acids in drone brood preparations and combined preparations based on it

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 389-393
Author(s):  
D.V. Mitrofanov ◽  
N.V. Budnikova
Keyword(s):  
Royal Jelly ◽  
Storage Conditions ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Strict Adherence ◽  
Drone Brood ◽  
Wide Range ◽  
Active Substances ◽  
Melanin Complex

The drone brood contains a large number of substances with antioxidant activity. These substances require stabilization and strict adherence to storage conditions. Among these substances are unique decenoic acids, the content of which is an indicator of the quality of drone brood and products based on it. The ability of drone brood to reduce the manifestations of oxidative stress is shown. There are dietary supplements for food and drugs based on drone brood, which are used for a wide range of diseases. Together with drone brood, chitosan-containing products, propolis, royal jelly can be used. They enrich the composition with their own biologically active substances and affect the preservation of the biologically active substances of the drone brood. Promising are the products containing, in addition to the drone brood, a chitin-chitosan-melanin complex from bees, propolis, royal jelly. The chitin-chitosan-melanin complex in the amount of 5% in the composition of the adsorbent practically does not affect the preservation of decenic acids, while in the amount of 2% and 10% it somewhat worsens. The acid-soluble and water-soluble chitosan of marine crustaceans significantly worsens the preservation of decenoic acids in the product. Drone brood with royal jelly demonstrates a rather high content of decenoic acids. When propolis is introduced into the composition of the product, the content of decenoic acids increases according to the content of propolis.

Barley seed storage under controlled conditions

2019 ◽  
pp. 140-150 ◽  
Author(s):  
O. A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov
Keyword(s):  
Hordeum Vulgare ◽  
Moisture Content ◽  
Spring Barley ◽  
Seed Storage ◽  
Growing Season ◽  
Storage Conditions ◽  
Meteorological Conditions ◽  
Hordeum Vulgare L ◽  
The Relationship ◽  
And Storage

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.

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