P6414DNA hydroxymethylation combined with carotid plaques as a novel biomarker for coronary atherosclerosis
Abstract Background Accumulating evidence has shown the association between DNA methylation and hydroxymethylation and the development of atherosclerosis. However, little is known about the diagnostic value of DNA methylation and hydroxymethylation for coronary atherosclerosis. Carotid plaque has a significant correlation with coronary atherosclerosis, which is a common marker for coronary atherosclerosis. Purpose The major aim of the present study is to determine whether DNA methylation and hydroxymethylation combined with carotid plaques can be useful to the diagnosis of coronary atherosclerosis. Methods 113 patients undergoing carotid ultrasound were enrolled into the study and measured the levels of 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC). Crouse score and Gensini score were used to evaluate the severity of carotid and coronary atherosclerosis, respectively. Results With the increasing of severity of carotid plaque and Crouse score, a stepwise upward trend was observed in 5-mC and 5-hmC levels, which were significantly correlated with the risk factors such as FPG and HbA1c levels, Crouse score and Gensini score. Crouse score and 5-hmC, not 5-mC, were the risk factors for coronary atherosclerosis after adjustment for the risk factors. Receiver operating characteristic (ROC) analysis for comparing their diagnostic value for coronary atherosclerosis indicated that 5-hmC combined with Crouse score was the diagnostic biomarker for coronary atherosclerosis, with the highest areas under the curve (AUC), valuable sensitivity and specificity. Logistic regression analysis Variables Univariate analysis Multivariate analysis OR (95% CI) P OR (95% CI) P History of DM 3.680 (1.645–8.235) 0.002 4.487 (0.227–88.680) 0.324 FPG (mmol/L) 1.782 (1.219–2.605) 0.003 0.972 (0.307–3.071) 0.961 HbA1c (%) 1.785 (1.191–2.674) 0.005 2.861 (0.717–11.422) 0.137 TC (mmol/L) 1.171 (0.913–1.502) 0.214 LDL-c (mmol/L) 1.383 (0.939–2.036) 0.100 hs-CRP (mg/L) 1.099 (1.020–1.184) 0.014 1.268 (1.013–1.588) 0.038 Crouse score 1.795 (1.445–2.230) <0.001 1.863 (1.053–3.297) 0.033 5-mC (%) 3.221 (1.851–5.604) <0.001 0.983 (0.386–2.505) 0.972 5-hmC (%) per 0.01% 1.484 (1.265–1.741) <0.001 1.767 (1.250–2.499) 0.001 Conclusions These findings suggest 5-hmC level combined with Crouse score may provide the meaningful information for coronary atherosclerosis diagnosis. Acknowledgement/Funding National Natural Science Foundation of China (No.81570212, No.31800976)