scholarly journals The antioxidant and physicochemical properties of microencapsulated bioactive compounds in Securigera securidaca (L.) seed extract by co-crystallization

2019 ◽  
Vol 3 (4) ◽  
pp. 243-250 ◽  
Author(s):  
Ali Behnam Nik ◽  
Mohsen Vazifedoost ◽  
Zohreh Didar ◽  
Bahareh Hajirostamloo
Keyword(s):  
Phenolic Compounds ◽  
Moisture Content ◽  
Bioactive Compounds ◽  
Production Efficiency ◽  
Radical Scavenging ◽  
Seed Extract ◽  
Angle Of Repose ◽  
Size Difference ◽  
Radical Scavenging Ability ◽  
Securigera Securidaca

Abstract Securigera securidaca seed is a good source of phenolic compounds with high antioxidant properties. Preservation and maintenance of natural antioxidants have always been a challenge and microencapsulation is a suitable method for this purpose. In this study, the chemical compounds of the plant seed extract were identified by GC/MS device. Bioactive compounds from the seed ethanolic extract were microencapsulated in the sucrose matrix during the co-crystallization process. The evaluations included total phenolic compounds, radical scavenging ability, production efficiency, moisture content, and flowability characteristics of the produced powders, such as compressibility index, Hausner ratio, and angle of repose. The results showed significant differences in the phenolic compounds and the radical scavenging ability between the control sample and the co-crystallized powder (P < 0.05). The production efficiency and the moisture content of extract-containing co-crystallized powder were 84% and 0.14%, respectively. The particle size difference of the microencapsulated powder could significantly affect the powder flowability characteristics (P < 0.05), and particles with a size of 1 mm showed better flowability behaviour. FT-IR charts for samples revealed chemical bonds specific to saccharose molecule indicating no changes in covalent bonds present in saccharose molecule structure after the process. Scanning electron microscope images showed the presence of vacant spaces and porosity in the structure of saccharose crystals formed during the process of crystallization. As a result, the co-crystallized powder obtained from the plant extract can be used as an appropriate antioxidant in the food and pharmaceutical formulations.

scholarly journals Bioactive Compounds in Latvian Beer

2013 ◽  
Vol 30 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Ilona Dabina-Bicka ◽  
Daina Karklina ◽  
Zanda Kruma ◽  
Fredijs Dimins
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Complex Mixture ◽  
Total Phenolic ◽  
Dpph Radical

Abstract Beer is a complex mixture - over 400 different compounds have been characterized in beer. Significant health and product quality promoting benefits have been attributed to its bioactive secondary metabolites such as phenolics. Polyphenols and phenolic acids present in beer are natural antioxidants. The aim of the research was to characterize the bioactive compounds in Latvian barley beer, such as phenolic acids and flavanols. In an experiment, different lager-type beers produced in Latvia were analysed. The total phenolic content was determined spectrophotometrically according to the Folin-Ciocalteu colorimetric method and expressed as gallic acid equivalents. Individual phenolic compounds were determined using high performance liquid chromatography (HPLC). The antioxidant potential of beer was analyzed by the 2,2-diphenyl-1-picrylhydraziyl (DPPH) radical assays and expressed as micromoles of Trolox equivalents. The research showed that the total phenolic content of dark beer samples (320.8-863.6 mg GE L-1) was mostly higher than that of the light beers (300.9-475.2 mg GE L-1). In total, eleven phenols were determined in the analysed samples. Also the sum of individual phenolics in dark beer samples was higher than in the light beer brands. All beer samples exhibited a strong DPPH radical scavenging activity: from 441.3 to 1064.2 μmol TE L-1 for the light beer samples, and from 726.2 to 1748.7 μmol TE L-1 for the dark beer. The research suggests that composition of beer phenolic compounds was not dependent on the type of beer - light or dark.

scholarly journals Plant Phenolics: Bioavailability as a Key Determinant of Their Potential Health-Promoting Applications

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1263
Author(s):  
Patricia Cosme ◽  
Ana B. Rodríguez ◽  
Javier Espino ◽  
María Garrido
Keyword(s):  
Phenolic Compounds ◽  
Biological Effects ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Plant Phenolics ◽  
Potential Health ◽  
Factors Affecting ◽  
Health Promoting ◽  
Radical Scavenging Ability ◽  
And Oxidative Stress

Phenolic compounds are secondary metabolites widely spread throughout the plant kingdom that can be categorized as flavonoids and non-flavonoids. Interest in phenolic compounds has dramatically increased during the last decade due to their biological effects and promising therapeutic applications. In this review, we discuss the importance of phenolic compounds’ bioavailability to accomplish their physiological functions, and highlight main factors affecting such parameter throughout metabolism of phenolics, from absorption to excretion. Besides, we give an updated overview of the health benefits of phenolic compounds, which are mainly linked to both their direct (e.g., free-radical scavenging ability) and indirect (e.g., by stimulating activity of antioxidant enzymes) antioxidant properties. Such antioxidant actions reportedly help them to prevent chronic and oxidative stress-related disorders such as cancer, cardiovascular and neurodegenerative diseases, among others. Last, we comment on development of cutting-edge delivery systems intended to improve bioavailability and enhance stability of phenolic compounds in the human body.

scholarly journals Antioxidant Activity and Bioactive Compounds of Lamium album Flower Extracts Obtained by Supercritical Fluid Extraction

2021 ◽  
Vol 11 (16) ◽  
pp. 7419
Author(s):  
Pascaline Aimee Uwineza ◽  
Anna Gramza-Michałowska ◽  
Marcin Bryła ◽  
Agnieszka Waśkiewicz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Array Detector ◽  
Frap Assay ◽  
Constant Flow Rate ◽  
Lamium Album

In this research, supercritical CO2 extraction is applied to extract bioactive compounds from Lamium album (white dead nettle, Lamiaceae). Extraction was performed at various temperatures (40, 50, and 60 °C) using methanol as co-solvent at a constant flow rate of CO2, methanol, and pressure. The collected extracts were characterized in terms of antioxidant capacity by using DPPH, ABTS and FRAP in vitro antioxidant activity assays, whereas the Folin–Ciocalteu procedure was employed to estimate the total phenols content (TPC). On the other hand, phenolic compounds in the extracts were quantitated by liquid chromatography coupled with a photodiode array detector (UPLC-PDA) and confirmed with a mass detector (TQD). The extracts have shown high TPC ranged between 234.17 to 650.17 mg GAE/g extract. DPPH scavenging of the extracts was estimated and obtained EC50 values ranged from 0.12 to 0.37 mg/mL of solution. The ABTS radical scavenging activity ranged from 43.20 to 44.53 µg TE/g. The FRAP value was found within the range of 19.48 to 44.74 µmol TE/g of extract. Differences between extraction conditions were observed. In this research, 50 °C/250 bar was efficient for the TPC, DPPH, ABTS, and FRAP assays; moreover, statistically, TPCs and FRAP assay showed significant differences between the conditions at α = 0.05. The identification of phenolic compounds in the obtained extract of Lamium album flowers, using UPLC/PDA, revealed that chrysin, pinostrobin, myricetin, and trans-3-hydroxycinnamic acid were the significant molecules present, which may be responsible for the high content of polyphenols and antioxidant activity. The results obtained indicated that SC-CO2 could be considered an alternative method for extracting bioactive compounds of Lamium album. High antioxidant activity and the presence of various bioactive compounds indicate the potential of this plant from the Lamiaceae family and the possibility of its application in various industries, including agriculture, food technology, or pharmacy.

Changes in Polyphenols Distribution and Antioxidant Activity during Fermentation of Some Underutilized Legumes

2009 ◽  
Vol 15 (1) ◽  
pp. 41-46 ◽  
Author(s):  
G. Oboh ◽  
A.O. Ademiluyi ◽  
A.A. Akindahunsi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phenol Content ◽  
Radical Scavenging Ability

The effect of fermentation on the polyphenol distribution and antioxidant activity of four underutilized legumes [Cajanus cajan L. Millsp (Pigeon pea), Vigna subterranea L. Verdc (Bambara groundnut), Sphenostylis stenocarpa Harms (African yam bean), and Phaseolus vulgaris L. (Kidney bean)] were investigated. The beans were cooked (12 h), soaked in boiled water (12 h), dehulled, and then cooked again (2 h). The cotyledons were drained, wrapped in jute sacks and left to ferment at 37°C (4 days) to produce condiments. The distribution of free and bound phenolic compounds in the fermented and unfermented beans was determined; thereafter the free radical scavenging ability, reducing power, and the ability of the free and bound phenolic compounds of the fermented and unfermented beans to inhibit lipid peroxidation were determined. The results of the study revealed that fermentation caused a significant increase (p<0.05) in the free soluble phenol content of the legumes, while there was a significant decrease (p<0.05) in the bound phenol content of the legumes. Free soluble phenol from both the fermented and unfermented legumes had a significantly (p<0.05) higher reducing power (except C. cajan), free radical scavenging ability (except C. cajan) and inhibition of lipid peroxidation than bound phenolic extract. However, free soluble phenolic compounds from the fermented beans had a significantly higher (p<0.05) reducing power, free radical scavenging ability, and inhibition of lipid peroxidation than free soluble phenols from unfermented beans. Hence, it was concluded that, fermentation could increase the free soluble phenolic content of the underutilized legumes tested and consequently enhance the antioxidant activities of the legumes; fermented V. subterranea was found to be the most promising condiment with antioxidant activity.

scholarly journals Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
María del Pilar Fernández-Poyatos ◽  
Gökhan Zengin ◽  
Carlos Salazar-Mendías ◽  
Antonio Ruiz-Medina ◽  
Kouadio Ibrahime Sinan ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Quantitative Analyses ◽  
Phenolic And Flavonoid

In this work, we report the phenolic composition and bioactivity of the aerial parts of three species of Sarcocapnos (S. enneaphylla, S. pulcherrima, and S. saetabensis) to study their potential as sources of bioactive compounds to revalorize them and contribute to the conservation of these plant species. Samples were collected in different locations in the province of Jaén (southeast of Spain), and qualitative and quantitative analyses of phenolic compounds were performed by high-performance liquid chromatography with diode array and mass spectrometry detection. S. enneaphylla presented the highest concentration of phenolic compounds (58 mg/g DE). The most abundant compound in S. enneaphylla and S. saetabensis was rutin (35 mg/g DE and 11.7 mg/g DE, respectively), whereas isorhamnetin-O-rutinoside was dominant in S. pulcherrima (11.5 mg/g DE). Several assays were performed to evaluate the potential bioactivity of the three species of Sarcocapnos. These assays included antioxidant and radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, and enzyme inhibitory activity (acetylcholinesterase, amylase, butyrylcholinesterase, glucosidase, and tyrosinase). In general, all methanolic extracts presented the highest phenolic and flavonoid contents, as well as the highest radical scavenging, antioxidant, and enzyme inhibitory properties. This relationship between phenolics and bioactivity was confirmed by multivariate analysis.

Recovery of Phenolic Compounds and Carbohydrates from Hydro-ethanolic Extract of Zizyphus lotus Fruit using Ultrafiltration Process

2017 ◽  
Vol 13 (12) ◽  
Author(s):  
Khaoula Mkadmini Hammi ◽  
Dorra Jellouli Ennigrou ◽  
Hatem Majdoub ◽  
Riadh Ksouri
Keyword(s):  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Cross Flow ◽  
Ethanolic Extract ◽  
Syringic Acid ◽  
Total Phenolic ◽  
Cellulose Membrane ◽  
Permeate Flux ◽  
Total Phenolic Compounds ◽  
Radical Scavenging Ability

AbstractCross flow ultrafiltration (UF) using a cellulose membrane (cut off of 5 kDa) was employed to separate phenolic compounds from carbohydrates of hydro-ethanolic extract obtained fromZizyphus lotusfruit. The performance of the UF membrane was evaluated in terms of permeate flux, fouling index and retention values of total phenolic compounds, carbohydrates and uronic acid contents. The results showed that at VRF of 4.5, the membrane gave the highest rejections towards carbohydrates (99.89 %) and uronic acids (82.8 %) and the lowest average rejection of total phenolic compounds (3.12 %). Moreover, the permeate fraction exhibited the highest 2,2-diphenyl-1-picrylhydrazyl free radical scavenging ability and total antioxidant capacity with 0.036 mg.mL−1and 102.145(mgGAE.g−1DM), respectively. This result is due to the presence of phenolic compounds (gallic acid, syringic acid, catechin, quercetin and kaempferol) as identified by HPLC system. Besides, a low rejection values of the UF membrane concerning these compounds were confirmed by liquid chromatography analyses.

The Effect of Ring Nitrogen Atoms on the Homolytic Reactivity of Phenolic Compounds: Understanding the Radical-Scavenging Ability of 5-Pyrimidinols

2003 ◽  
Vol 9 (20) ◽  
pp. 4997-5010 ◽  
Author(s):  
Luca Valgimigli ◽  
Giovanni Brigati ◽  
Gian Franco Pedulli ◽  
Gino A. DiLabio ◽  
Marina Mastragostino ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Ring Nitrogen ◽  
Radical Scavenging Ability

scholarly journals Antioxidant properties of selected culinary spices

2016 ◽  
Vol 62 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Marta Słowianek ◽  
Joanna Leszczyńska
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Food Preservatives ◽  
Phenolic Contents ◽  
Radical Scavenging Ability

SummaryIntroduction: Seasonings added to food enhance its flavor and texture. Some of them can also extend their shelf-lives thanks to the presence of antioxidant compounds. Objective: The aim of the study was to investigate twenty eight commercially available spices for the total phenolic contents and antioxidant activity. Methods: Total phenols were estimated according to the Folin-Ciocalteu method. Antioxidant activities of the extracts were determined with DPPH assay. Results: Our results showed that the most of analysed spices are rich in phenolic compounds and demonstrate good antioxidant activity. The total polyphenol content oscillated around 0.9-155.1 mg GAE/g with the lowest value for sesame and the highest for cinnamon. The DPPH radical scavenging ability expressed as % ranged from 4.1% for sesame to 94.9% for cloves. Moreover, a moderate correlation (r=0.63, p<0.05) was reported between antioxidant activity and total phenolics, revealing that phenolic compounds are the important antioxidant components in the examined spices. Conclusion: The study shows also that a lot of spices can serve as food preservatives and, at the same time, have a beneficial effect on human health.

scholarly journals Bioactive Compounds and Antioxidant Properties of Banana Inflorescence in a Beverage for Maternal Breastfeeding

2020 ◽  
Vol 11 (1) ◽  
pp. 343
Author(s):  
Doungporn Amornlerdpison ◽  
Vachira Choommongkol ◽  
Kanjana Narkprasom ◽  
Susanha Yimyam
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Microbial Pathogens ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Health Food

Banana inflorescence is consumed as a traditional Thai cuisine for milk lactation in maternal breastfeeding. In this study, the inflorescence of banana (Musa x paradisiaca) was extracted in various solvents to determine the bioactive compounds and antioxidant activity in 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging. A suitable extract was developed into a beverage. We compared the results for the amount of total phenolic compounds and the capability of antioxidants obtained in polar and non-polar solvents. The extract in a high-polarity solvent demonstrated high total phenolic compounds and flavonoids. The bioactive compounds of banana inflorescence contained β- sitosterol, flavonoids, saponin, and other phenolic compounds such as catechin and isoquercetin. The aqueous extract of banana inflorescence was developed to act as a primary beverage ingredient. The beverage containing the aqueous extract of banana inflorescence (BAB) exhibited a brownish-yellow color and displayed high acidity and high total phenolic compounds, which are responsible for the antioxidant activity. The food processing of BAB showed no contamination of microbial pathogens. From our results, we concluded that banana inflorescence is a beneficial health food supplement for general consumers. Additionally, the beverage provides convenience and an alternative drink for postpartum mothers who breastfeed for their infants.

scholarly journals Bioactive compounds from Syzygium malaccense leaves: optimization of the extraction process, biological and chemical characterization

2020 ◽  
Vol 42 ◽  
pp. e46773 ◽  
Author(s):  
Aline Savi ◽  
Matheus Augusto Calegari ◽  
Gabrielle Cristina Calegari ◽  
Vidiany Aparecida Queiroz Santos ◽  
Denise Wermuth ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Radical Scavenging Ability

Syzygium malaccense belongs to the family Myrtaceae and it is popularly recognised as ‘jambo’ in Brazil. It is poorly studied regarding the bioactive compounds of fruits and leaves. This study aimed at optimizing the extraction and characterization of bioactives compounds from S. malaccense leaves. Extraction was optimized using a 23 full factorial design to evaluate the effect of ethanol concentration, time and temperature on polyphenols and antioxidants contents. Analyses of phenolic compounds content (Folin-Ciocalteau and HPLC-DAD), in vitro antioxidant capacities (DPPH, ABTS and FRAP), antimicrobial activity against bacteria and yeasts of ethanolic extract was carried out in optimal conditions of extraction. The optimized extraction condition was 45 min., 80°C and 40:60 (v v-1) ethanol:water. The extract showed high total phenolic content (0.074 mg GAE kg-1), DPPH (0.666 μmol TE kg-1) and ABTS (0.853 μmol TE kg-1) free radical scavenging ability and appreciable ferric reducing power (1.267 μmol Fe2+ kg-1). HPLC methodology allowed the identification of four bioactive compounds: gallic acid (0.00036 mg kg-1), catechin (0.00021 mg kg-1), rutin (0.00027 mg kg-1) and quercetin (0.00003 mg kg-1). The extract showed inhibitory activity against S. aureus (1 µg L-1); S. bongori (8 µg L-1), C. albicans and C. tropicalis (2 µg L-1). It also showed microbicidal potential against S. aureus; C. albicans and C. tropicalis.

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