Sustained Cross-reactive Antibody Responses After Human Papillomavirus Vaccinations: Up to 12 Years Follow-up in the Finnish Maternity Cohort

2020 ◽  
Author(s):  
Hanna Kann ◽  
Matti Lehtinen ◽  
Tiina Eriksson ◽  
Heljä-Marja Surcel ◽  
Joakim Dillner ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Ammonium Thiocyanate ◽  
Human Papillomaviruses ◽  
Antibody Responses ◽  
Quadrivalent Vaccine ◽  
Hpv Vaccines ◽  
Antibody Avidity ◽  
Hpv Types ◽  
Antibody Levels ◽  
Reactive Antibody

Abstract Background Human papillomaviruses (HPV) cause several human cancers. Bivalent (Cervarix) and quadrivalent (qGardasil) HPV vaccines both contain virus-like particles of the major oncogenic HPV types 16 and 18, but also cross-protect against some nonvaccine types. However, data on long-term sustainability of the cross-reactive antibody responses to HPV vaccines are scarce. Methods Serum samples donated 7–12 years after immunization at age 16–17 years with bivalent (n = 730) or quadrivalent (n = 337) HPV vaccine were retrieved from the population-based Finnish Maternity Cohort biobank. Serum antibody levels against HPV types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, and 73 were determined using multiplex pseudovirion binding assay. Antibody avidity was assessed using ammonium thiocyanate treatment. Results Seropositivity for HPV31, 33, 35, 45, 51, 52, 58, 59, 68, and 73 was increasingly common (P ≤ .001; χ 2 test for trend for each of these types) when women had high anti-HPV16 antibody levels. For 8 nonvaccine HPV types seropositivity was more common among recipients of bivalent than quadrivalent vaccine, in particular for HPV31, 35, 45, 51, 52, and 58 (P < .001). Antibody avidity was higher in the quadrivalent vaccine recipients for HPV6, 11, and two of the nonvaccine types, but lower for HPV16 and 18 (P < .001). Conclusions Both vaccines elicit cross-reactive antibodies detectable even 12 years after vaccination. Cross-reactive seropositivity is more common in women with high anti-HPV16 antibody response and in the bivalent vaccine recipients.

scholarly journals Development and evaluation of a new non-competitive Luminex immunoassay detecting antibodies against human papillomavirus types 6, 11, 16 and 18

2021 ◽  
Vol 102 (5) ◽  
Author(s):  
Andrea Trevisan ◽  
Michel D. Wissing ◽  
Carole Dagenais ◽  
Pierre Forest ◽  
Agnihotram V. Ramanakumar ◽  
...  
Keyword(s):  
Solid Phase ◽  
Natural Infection ◽  
Serum Antibody ◽  
Area Under The Curve ◽  
Correlation Coefficients ◽  
Human Papillomaviruses ◽  
Quadrivalent Vaccine ◽  
Igg Antibody ◽  
Hpv Dna ◽  
Antibody Levels

Serum antibody levels can be used to measure the humoral immune response against human papillomaviruses (HPV). We developed and validated a rapid, technically simple and relatively inexpensive multiplex non-competitive Luminex-based immunoassay (ncLIA) to measure total IgG antibody levels against four HPV types. For the assay’s solid phase, virus-like particles (VLPs) of HPV6, 11, 16 and 18 were bound to heparin-coated beads. HPV serum antibody levels binding to the VLPs were quantified using a phycoerithrin-conjugated secondary polyclonal donkey anti-human IgG antibody. Standardization and validation of the ncLIA were performed using 96 paired serum and genital samples from participants in the HITCH cohort study, including young women (aged 18–24 years) and their male sexual partners (aged 18+) in Montreal, Canada. Results from the ncLIA were compared to a validated Luminex immunoassay from PPD laboratories using Pearson’s correlation coefficients, receiver operating characteristic curves and logistic regression. Our assay had good inter- and intra-assay variability. The correlation of serum antibody levels between the ncLIA and validation assay was highest for HPV16 and HPV11 (r=0.90), followed by HPV6 (r=0.86) and HPV18 (r=0.67). The ncLIA was better able to predict HPV DNA positivity in genital samples than the validation assay for HPV16 [area under the curve (AUC) 0.65 versus 0.52, P=0.001] and HPV18 [AUC 0.71 versus 0.57, P=0.024]. AUCs for HPV6 and HPV11 were similar between the two assays (0.70 versus 0.71, P=0.59, and 0.88 versus 0.96, P=0.08, respectively). The developed ncLIA is useful for measuring total IgG antibody response following natural infection or vaccination against four HPV VLPs included in the quadrivalent vaccine.

scholarly journals Low Immunogenicity of Intravesical Phage Therapy for Urogenitary Tract Infections

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 627
Author(s):  
Sławomir Letkiewicz ◽  
Marzanna Łusiak-Szelachowska ◽  
Ryszard Międzybrodzki ◽  
Maciej Żaczek ◽  
Beata Weber-Dąbrowska ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Bacterial Infections ◽  
Phage Therapy ◽  
Serum Antibody ◽  
Multidrug Resistant ◽  
Antibody Responses ◽  
Urogenital Infections ◽  
Reliable Model ◽  
Tract Infections ◽  
Antibody Levels

Patients with chronic urinary and urogenital multidrug resistant bacterial infections received phage therapy (PT) using intravesical or intravesical and intravaginal phage administration. A single course of PT did not induce significant serum antibody responses against administered phage. Whilst the second cycle of PT caused a significant increase in antibody levels, they nevertheless remained quite low. These data combined with good therapy results achieved in some patients suggest that this mode of PT may be an efficient means of therapy for urogenital infections and a reliable model for a clinical trial of PT.

scholarly journals A genetic basis for atrophy: dominant non-responsiveness and helicobacter induced gastritis in F1 hybrid mice

Gut ◽  
1999 ◽  
Vol 45 (3) ◽  
pp. 335-340 ◽  
Author(s):  
P Sutton ◽  
J Wilson ◽  
R Genta ◽  
D Torrey ◽  
A Savinainen ◽  
...  
Keyword(s):  
Immune Response ◽  
Serum Antibody ◽  
Antibody Responses ◽  
Host Factors ◽  
Mouse Strains ◽  
F1 Hybrid ◽  
Helicobacter Felis ◽  
Humoral Immune ◽  
Antibody Levels ◽  
Hybrid Mice

BACKGROUNDThe importance of host factors in helicobacter induced gastritis has been shown in animal models. Infection of most mouse strains withHelicobacter felis results in a functional atrophic gastritis, while other strains remain gastritis free.AIMSTo investigate these host factors further by using genetic crosses of responder and non-responder mice.METHODSF1 hybrids of the non-responder CBA/Ca strain and three strains of mice known to develop H felis induced gastritis were infected for three months with H felis. Gastritis was assessed by histopathology and serum antibody responses by ELISA.RESULTSInfection of CBA/Ca mice and F1 hybrids induced little or no gastritis. Analyses of the antibody responses in these mice revealed virtually undetectable anti-helicobacter antibody levels despite colonisation with high numbers of H felis. In contrast, infection of H felis responsive strains induced gastritis and a significant humoral immune response.CONCLUSIONSThe non-responsiveness of CBA/Ca mice to H felis infection is dominantly inherited. The lack of gastritis in CBA mice and their offspring is probably due to active suppression of the immune response normally mounted against H felis. Investigation of these mechanisms will provide important insights relevant to induction of gastric atrophy and cancer in humans.

scholarly journals HPV Vaccines: Current Status

2016 ◽  
pp. 1
Author(s):  
Mustafa Erkan Sarı ◽  
Mehmet Mutlu Meydanlı
Keyword(s):  
Cervical Cancer ◽  
Dose Schedule ◽  
Genital Warts ◽  
Hpv Infection ◽  
World Health ◽  
Quadrivalent Vaccine ◽  
Current Status ◽  
Hpv Vaccines ◽  
Cervical Lesions ◽  
Hpv Types

<p><strong>Abstract</strong></p><p>Cervical cancer is the fourth most common cancer in women worldwide, and the main cause is Human Papillomavirus (HPV) infection. HPV vaccines have had dramatic impacts on the prevalence of targeted HPV types (6,11,16 and 18), genital warts and precancerous cervical lesions. The World Health Organization (WHO) and Centers for Disease Control and Prevention (CDC) have confirmed the safety of HPV vaccines after &gt;250 million doses were administered worldwide. WHO approved the two-dose-schedule of HPV vaccines in females younger than 15 years of age, with ≥6 month intervals. Extension of vaccination to men could further reduce the population prevalence of HPV and provide direct protection of men against genital warts and anal, penile and oropharyngeal cancers. The nine-valent HPV vaccine has demonstrated equivalent protection against the four types in the quadrivalent vaccine and high efficacy against the next five commonest causes of cervical cancer (HPV types 31,33,45,52 and 58). The Advisory Committee on Immunization Practices (ACIP) recommends the nine-valent vaccine and it has been approved by the FDA in 2014 for both genders between 11–12 years of age.</p><p> </p>

scholarly journals Cross-reactive antibody responses against SARS-CoV-2 and seasonal common cold coronaviruses

2020 ◽  
Author(s):  
Shelley Klompus ◽  
Sigal Leviatan ◽  
Thomas Vogl ◽  
Iris Kalka ◽  
Anastasia Godneva ◽  
...  
Keyword(s):  
T Cells ◽  
High Resolution ◽  
Common Cold ◽  
Interindividual Variability ◽  
Serum Antibody ◽  
Cross Reactivity ◽  
Antibody Responses ◽  
Antibody Repertoires ◽  
High Throughput Assay ◽  
Reactive Antibody

Beyond SARS-CoV-2, six more coronaviruses infect humans (hCoVs), four of which cause only mild symptoms (seasonal/common cold hCoVs). Previous exposures to seasonal hCoVs may elicit immunological memory that could benefit the course of SARS-CoV-2 infections. While cross-reactive T cells epitopes of SARS-CoV-2 and seasonal hCoVs have been reported in individuals unexposed to SARS-CoV-2, potential antibody-based cross-reactivity is incompletely understood. Here, we have probed for high resolution antibody binding against all hCoVs represented as 1,539 peptides with a phage-displayed antigen library. We detected broad serum antibody responses against peptides of seasonal hCoVs in up to 75% of individuals. Recovered COVID-19 patients exhibited distinct antibody repertoires targeting variable SARS-CoV-2 epitopes, and could be accurately classified from unexposed individuals (AUC=0.96). Up to 50% of recovered patients also mounted antibody responses against unique epitopes of seasonal hCoV-OC43, that were not detectable in unexposed individuals. These results indicate substantial interindividual variability and antibody cross-reactivity between hCoVs from the direction of SARS-CoV-2 infections towards seasonal hCoVs. Our accurate high throughput assay allows profiling preexisting antibody responses against seasonal hCoVs cost-effectively and could inform on their protective nature against SARS-CoV-2.

scholarly journals Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immunized against the Protozoan Parasite Ichthyophthirius multifiliis

2003 ◽  
Vol 10 (5) ◽  
pp. 876-881 ◽  
Author(s):  
Joanne L. Maki ◽  
Harry W. Dickerson
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Serum Antibody ◽  
Protozoan Parasite ◽  
Mean Value ◽  
Infected Fish ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ichthyophthirius Multifiliis ◽  
Antibody Levels

ABSTRACT Fish acquire protective immunity against the ciliated protozoan parasite Ichthyophthirius multifiliis following sublethal infection or inoculation with I. multifiliis immobilization antigens (i-antigens). In both cases, parasite-immobilizing antibodies have been identified in sera and mucosal secretions. To investigate the kinetics of this immune response, antibody levels were determined by enzyme-linked immunosorbent assay (ELISA) in the sera and cutaneous mucus of channel catfish (Ictalurus punctatus) that were either infected with parasites or given a single injection of purified i-antigen (5.0 μg/fish) in Freund's incomplete adjuvant. At 5 weeks, infected and inoculated fish had a mean serum (1:80 dilution) antibody absorbance (A 405) value of 0.54 ± 0.17 and 0.35 ± 0.03, respectively, which were significantly higher (α = 0.05) than the pretreatment serum (1:80 dilution) antibody absorbance value of 0.24 ± 0.05. At 14 weeks, mean serum (1:80 dilution) ELISA absorbance values in the teo groups of fish increased to 0.79 ± 0.30 and 0.71 ± 0.24, respectively. In both groups of fish, antibody levels in cutaneous mucus (undiluted) were much lower than those in sera. Infected fish had detectable mucus (undiluted) antibody levels from 3 to 9 weeks, with the highest mean value (0.30 ± 0.07) occurring at 7 weeks. Although individual inoculated fish produced serum antibody absorbance values comparable to those seen in infected fish, the mean mucus antibody values in this group did not rise above pretreatment levels. I. multifiliis infection induced a transient mucosal antibody response that coincided with the resolution of infection. Whether elicited by infection or intraperitoneal injection of i-antigen, the serum and mucus antibody responses of channel catfish immunized against I. multifiliis did not occur synchronously.

scholarly journals Antibody Responses in Patients with Staphylococcal Septicemia against Two Staphylococcus aureus Fibrinogen Binding Proteins: Clumping Factor and an Extracellular Fibrinogen Binding Protein

2000 ◽  
Vol 7 (1) ◽  
pp. 14-20 ◽  
Author(s):  
Patricia Colque-Navarro ◽  
Marco Palma ◽  
Bo Söderquist ◽  
Jan-Ingmar Flock ◽  
Roland Möllby
Keyword(s):  
Staphylococcus Aureus ◽  
Antibody Response ◽  
Binding Proteins ◽  
Serum Antibody ◽  
Antibody Responses ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Positive Serology ◽  
Fibrinogen Binding ◽  
Antibody Levels

ABSTRACT We analyzed the serum antibody responses against twoStaphylococcus aureus fibrinogen binding proteins, the cell-bound clumping factor (Clf) and an extracellular fibrinogen binding protein (Efb). The material consisted of 105 consecutive serum samples from 41 patients suffering from S. aureussepticemia and 72 serum samples from healthy individuals. An enzyme-linked immunosorbent assay (ELISA) was developed. Healthy individuals showed variable levels of antibodies against the studied antigens, and cutoff levels (upper 95th percentile) against these antigens were determined. No correlation was seen between serum antibody levels against Clf and Efb. In acute-phase samples 27% of patients showed positive antibody levels against Clf and 10% showed positive levels against Efb, while in convalescent-phase samples 63% (26 of 41) showed a positive serology against Clf and 49% (20 of 41) showed a positive serology against Efb. Antibody levels against Efb were significantly lower in the acute-phase sera than in sera from healthy individuals (P = 0.002). An antibody response against Clf was most frequent in patients suffering from osteitis plus septic arthritis and from endocarditis (80% positive). The antibody response against Efb appeared to develop later in the course of disease. A possible biological effect of measured antibodies was demonstrated with the help of an inhibition ELISA, in which both high-titer and low-titer sera inhibited the binding of bacteria to fibrinogen. In conclusion, we have demonstrated in vivo production ofS. aureus fibrinogen binding proteins during deep S. aureus infections and a possible diagnostic and prophylactic role of the corresponding serum antibodies in such infections.

Immunoepidemiology of Ascaris lumbricoides: relationships between antibody specificities, exposure and infection in a human community

Parasitology ◽  
1992 ◽  
Vol 104 (1) ◽  
pp. 153-159 ◽  
Author(s):  
M. R. Haswell-Elkins ◽  
H. Leonard ◽  
M. W. Kennedy ◽  
D. B. Elkins ◽  
R. M. Maizels
Keyword(s):  
Ascaris Lumbricoides ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Serum Antibody ◽  
Antibody Responses ◽  
Recent Exposure ◽  
Age Related ◽  
Sds Page ◽  
Antibody Specificities ◽  
Antibody Levels

SUMMARYThe serum antibody responses of 124 people naturally exposed to Ascaris lumbricoides infection were analysed by immunoprecipitation of radio-isotope labelled 3rd- and 4th-stage larval Ascaris suum excretory and secretory antigens (L3/4 ES). Profiles of antigens recognized were visualized by polyacrylamide gel electrophoresis (SDS–PAGE), and the band intensities of the 12 major precipitated antigens were individually scored. Most subjects were seropositive, but considerable variation was observed in the amount of total and individual ES antigens precipitated. The sex- and age-related profiles of antibody levels followed similar patterns to those of egg output. In addition, total antibody scores of individuals were closely correlated (r = 0.47–0.52) with their eggs per gram of faeces (e.p.g.) collected 4 months after blood samples were taken. These findings suggest that antibody levels against larval ES antigens reflect recent exposure and are consistent with the hypothesis that establishment of adult worms is proportional to the number of larvae that recently migrated through the lung.

scholarly journals Serum Hemagglutination Inhibition Activity Correlates with Protection from Gastroenteritis in Persons Infected with Norwalk Virus

2011 ◽  
Vol 19 (2) ◽  
pp. 284-287 ◽  
Author(s):  
Rita Czakó ◽  
Robert L. Atmar ◽  
Antone R. Opekun ◽  
Mark A. Gilger ◽  
David Y. Graham ◽  
...  
Keyword(s):  
Blood Group ◽  
Hemagglutination Inhibition ◽  
Serum Antibody ◽  
Blood Group Antigen ◽  
Antibody Responses ◽  
Norwalk Virus ◽  
Inhibition Activity ◽  
Whitney Test ◽  
Antibody Levels ◽  
Blocking Assay

ABSTRACTA hemagglutination inhibition (HAI) assay to assess serum antibody responses following Norwalk virus (NV) infection was developed. HAI activity increased significantly in individuals experimentally infected with NV (n= 18) and correlated with antibody levels measured in a histo-blood group antigen (HBGA) blocking assay. Prechallenge HAI antibody levels also correlated with protection from the development of gastroenteritis (Mann-Whitney test,P= 0.02). The HAI assay is another assay suitable for the detection of antibody that correlates with protection from Norwalk virus-associated disease.

scholarly journals Immunization with Bovine Serum Albumin (BSA) in Oil-Adjuvant Elicits IgM Antibody Response in Chinese Soft-Shelled Turtle (Pelodiscus Sinensis)

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 257
Author(s):  
Cheng Xu ◽  
Jiehao Xu ◽  
Yu Chen ◽  
Øystein Evensen ◽  
Hetron Mweemba Munang’andu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Large Scale ◽  
Vaccine Development ◽  
Serum Antibody ◽  
Disease Diagnosis ◽  
Antibody Responses ◽  
Pelodiscus Sinensis ◽  
Antibody Levels

Immunoassays are among the frontline methods used for disease diagnosis and surveillance. Despite this, there are no immunoassays developed for the Chinese soft-shelled turtle (Pelodiscus sinensis), which has expanded into large scale commercial production in several Asian countries. One of the critical factors delaying the development of immunoassays is the lack of characterized soft-shelled turtle immunoglobulins. Herein, we used mass spectrometry together with the ProtQuest software to identify the soft-shelled turtle IgM heavy chain in serum, which again was used to produce a polyclonal anti-turtle-IgM in rabbits. Thereafter, the polyclonal anti-turtle-IgM was used as a secondary antibody in an indirect ELISA to evaluate antibody responses of soft-shelled turtles injected with the bovine serum albumin (BSA) model antigen. Our findings show that only turtle immunized with a water-in-oil BSA plus ISA 763A VG adjuvant (SEPPIC, France) emulsion had antibodies detected at 42 days post vaccination (dpv) while turtles injected with phosphate buffered saline (PBS) only as well as turtle injected with BSA dissolved in PBS had no significant antibody levels detected in serum throughout the study period. In summary, our findings show that rabbit polyclonal anti-turtle-IgM produced can be used in ELISA to measure serum antibody responses in immunized soft-shelled turtles. Future studies should explore its application in other immunoassays needed for the disease diagnosis and vaccine development for soft-shelled turtles.

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