Cacodylate sensor and its application for the determination of amino acid levels in biological samples

2020 ◽  
Author(s):  
Hisham S M Abd-Rabboh ◽  
Ayman H Kamel ◽  
Fuziah H A Alshehri
Keyword(s):  
Biological Samples ◽  
Electrochemical Sensors ◽  
Response Times ◽  
Reference Electrode ◽  
High Sensitivity ◽  
Life Time ◽  
Polymeric Membrane ◽  
Time Signal ◽  
Sensitivity And Selectivity ◽  
Environmental And Biological Samples

Abstract Background The importance of recognizing and quantifying chemical anions/cations found in various types of samples, including environmental and biological samples, is extensively studied. Recent findings suggest the possibility of health risk caused by organic dimethylarsininc acid (DMAs) compound itself, not its arsenic inorganic metabolite. Objective This article aims to fabricate polymeric-membrane electrochemical sensors with high sensitivity and selectivity for dimethylarsinate (cacodylic acid sodium salt, DMAs) based on silver diethyldithiocarbamate (AgDDTC) and CuIIphthalocyanine (CuPC) as novel neutral carriers and their applications. Methods DMAs calibration relations and titrations were carried out using a potentiometric workstation equipped with a double-junction reference electrode, in conjunction with the fabricated working electrodes. Results Sensors revealed fast and stable anionic response with near-Nernstian slopes (-38.6 ± 0.9 and -31.5 ± 0.6 mV/decade), within concentration ranges (1.7x10−5 - 1.0x10−2 and 3.0x10−5 - 1.0x10−2 M) and detection limits (1.0x10−5 and 1.6x10−5 M) for AgDDTC- and CuPC-based sensors, respectively. Sensors are characterized with their extended life-time, signal stability, high precision and short response times. Selectivity for cacodylate anion over most common anions is tested for proposed electrodes. Sensors were satisfactorily applied for DMAs quantification in biological matrices with recoveries ranging between 96.2 and 99.0%. Membrane sensors were interfaced with a flow-through system for continuous monitoring of DMAs. The sensors were tested for the assay of different amino acids based on their reaction with cacodylate, where reaction end-points were monitored with the proposed electrodes using direct potentiometric determination and flow injection analysis (FIA).

scholarly journals Label-Free Split Aptamer Sensor for Femtomolar Detection of Dopamine by Means of Flexible Organic Electrochemical Transistors

Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2577 ◽  
Author(s):  
Yuanying Liang ◽  
Ting Guo ◽  
Lei Zhou ◽  
Andreas Offenhäusser ◽  
Dirk Mayer
Keyword(s):  
Detection Limit ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Label Free ◽  
Dopamine Detection ◽  
Electrochemical Transistor ◽  
Sensitivity And Selectivity

The detection of chemical messenger molecules, such as neurotransmitters in nervous systems, demands high sensitivity to measure small variations, selectivity to eliminate interferences from analogues, and compliant devices to be minimally invasive to soft tissue. Here, an organic electrochemical transistor (OECT) embedded in a flexible polyimide substrate is utilized as transducer to realize a highly sensitive dopamine aptasensor. A split aptamer is tethered to a gold gate electrode and the analyte binding can be detected optionally either via an amperometric or a potentiometric transducer principle. The amperometric sensor can detect dopamine with a limit of detection of 1 μM, while the novel flexible OECT-based biosensor exhibits an ultralow detection limit down to the concentration of 0.5 fM, which is lower than all previously reported electrochemical sensors for dopamine detection. The low detection limit can be attributed to the intrinsic amplification properties of OECTs. Furthermore, a significant response to dopamine inputs among interfering analogues hallmarks the selective detection capabilities of this sensor. The high sensitivity and selectivity, as well as the flexible properties of the OECT-based aptasensor, are promising features for their integration in neuronal probes for the in vitro or in vivo detection of neurochemical signals.

Highly Hg2+-sensitive and selective fluorescent sensors in aqueous solution and sensors-encapsulated polymeric membrane

RSC Advances ◽  
2016 ◽  
Vol 6 (13) ◽  
pp. 10401-10411 ◽  
Author(s):  
Sasiwimon Kraithong ◽  
Pattareeya Damrongsak ◽  
Kullatat Suwatpipat ◽  
Jitnapa Sirirak ◽  
Pattanawit Swanglap ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Detection Limits ◽  
High Sensitivity ◽  
Fluorescent Sensors ◽  
Polymeric Membranes ◽  
Polymeric Membrane ◽  
Sensitivity And Selectivity

The sensors in solutions and sensors encapsulated polymeric membranes exhibited high sensitivity and selectivity for Hg2+ detection, with detection limits of 0.2–49 ppb.

Sensitive and selective detection of microRNA in complex biological samples based on protein-enhanced fluorescence anisotropy

2020 ◽  
Vol 12 (5) ◽  
pp. 687-692 ◽  
Author(s):  
Yan Li ◽  
Yao Sun ◽  
Jieyun Ye ◽  
Fengping Pan ◽  
Bo Peng ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Biological Samples ◽  
Fluorescence Anisotropy ◽  
High Sensitivity ◽  
Selective Detection ◽  
Enhanced Fluorescence ◽  
Sensitivity And Selectivity

A molecular mass amplifying strategy to construct a fluorescence anisotropy sensor for the detection of microRNA with high sensitivity and selectivity in complex biological samples was proposed.

scholarly journals Nanomaterials-Based Electrochemical Sensors for In Vitro and In Vivo Analyses of Neurotransmitters

2018 ◽  
Vol 8 (9) ◽  
pp. 1504 ◽  
Author(s):  
Sharmila Durairaj ◽  
Boopathi Sidhureddy ◽  
Joseph Cirone ◽  
Aicheng Chen
Keyword(s):  
Electrochemical Sensors ◽  
Point Of Care ◽  
Electrochemical Techniques ◽  
High Sensitivity ◽  
Electrochemical Analysis ◽  
Detection Techniques ◽  
Efficient Detection ◽  
Sensitivity And Selectivity

Neurotransmitters are molecules that transfer chemical signals between neurons to convey messages for any action conducted by the nervous system. All neurotransmitters are medically important; the detection and analysis of these molecules play vital roles in the diagnosis and treatment of diseases. Among analytical strategies, electrochemical techniques have been identified as simple, inexpensive, and less time-consuming processes. Electrochemical analysis is based on the redox behaviors of neurotransmitters, as well as their metabolites. A variety of electrochemical techniques are available for the detection of biomolecules. However, the development of a sensing platform with high sensitivity and selectivity is challenging, and it has been found to be a bottleneck step in the analysis of neurotransmitters. Nanomaterials-based sensor platforms are fascinating for researchers because of their ability to perform the electrochemical analysis of neurotransmitters due to their improved detection efficacy, and they have been widely reported on for their sensitive detection of epinephrine, dopamine, serotonin, glutamate, acetylcholine, nitric oxide, and purines. The advancement of electroanalytical technologies and the innovation of functional nanomaterials have been assisting greatly in in vivo and in vitro analyses of neurotransmitters, especially for point-of-care clinical applications. In this review, firstly, we focus on the most commonly employed electrochemical analysis techniques, in conjunction with their working principles and abilities for the detection of neurotransmitters. Subsequently, we concentrate on the fabrication and development of nanomaterials-based electrochemical sensors and their advantages over other detection techniques. Finally, we address the challenges and the future outlook in the development of electrochemical sensors for the efficient detection of neurotransmitters.

scholarly journals Tyrosinase/Chitosan/Reduced Graphene Oxide Modified Screen-Printed Carbon Electrode for Sensitive and Interference-Free Detection of Dopamine

2019 ◽  
Vol 9 (4) ◽  
pp. 622 ◽  
Author(s):  
Cheng-You Liu ◽  
Yi-Chieh Chou ◽  
Jui-Hsuan Tsai ◽  
Tzu-Ming Huang ◽  
Jian-Zhang Chen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Sensitivity And Selectivity ◽  
Cv Measurements ◽  
Screen Printed

Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection’s specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4–8 μM and 40–500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.

scholarly journals Potentiometric Signal Transduction for Selective Determination of 1-(3-Chlorophenyl)piperazine “Legal Ecstasy” Through Biomimetic Interaction Mechanism

Chemosensors ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 46
Author(s):  
Eman El-Naby
Keyword(s):  
Dynamic Range ◽  
Limit Of Detection ◽  
Interaction Mechanism ◽  
High Sensitivity ◽  
Fast Response ◽  
Polymeric Membrane ◽  
Selective Determination ◽  
Sensitivity And Selectivity ◽  
Pharmacologically Active

1-(3-chlorophenyl)piperazine (mCPP) is a wide spread new psychoactive substance produces stimulant and hallucinogenic effects similar to those sought from ecstasy. Hence, in the recent years, mCPP has been introduced by the organized crime through the darknet as a part of the illicit ecstasy market with a variable complex profile of pharmacologically active substances that pose problematic risk patterns among people who take these seized products. Accordingly, the design of selective sensors for the determination of mCPP is a very important demand. In this respect, a supramolecular architecture; [Na(15-crown-5)][BPh4] from the assembly of 15-crown-5 and sodium tetraphenylboron has been utilized as an ionophore, for the first time in the selective recognition of mCPP in conjunction with potassium tetrakis(p-chlorophenyl)borate and dioctylphthalate through polymeric membrane ion sensors. The ionophore exhibited a strong binding affinity that resulted in a high sensitivity with a slope closed to the ideal Nernstian value; 58.9 ± 0.43 mV/decade, a larger dynamic range from 10−6 to 10−2 M, a lower limit of detection down to 5.0 × 10−7 M and a fast response time of 5 s. Very important also is it was afforded excellent selectivity towards mCPP over psychoactive substances of major concern, providing a potentially useful system for the determination of mCPP in the illicit market. On comparison with the natural β-cyclodextrin as an ionophore, it exhibited more sensitivity and selectivity estimated to be the superior.

High sensitivity and selectivity in quantitative analysis of drugs in biological samples using 4-column multidimensional micro-UHPLC-MS enabling enhanced sample loading capacity

2017 ◽  
Vol 989 ◽  
pp. 104-111 ◽  
Author(s):  
Ronald de Vries ◽  
Liesbeth Vereyken ◽  
Isabelle François ◽  
Lieve Dillen ◽  
Rob J. Vreeken ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Biological Samples ◽  
High Sensitivity ◽  
Loading Capacity ◽  
Sensitivity And Selectivity

scholarly journals Chemical-free and scalable process for the fabrication of a uniform array of liquid-gated CNTFET, evaluated by KCl electrolyte

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pankaj B. Agarwal ◽  
Navneet Kumar Thakur ◽  
Rishi Sharma ◽  
Parul Singh ◽  
Joshy Joseph ◽  
...  
Keyword(s):  
Field Effect Transistors ◽  
Reference Electrode ◽  
High Sensitivity ◽  
Label Free ◽  
Practical Applications ◽  
Lithography Process ◽  
Free Process ◽  
Label Free Detection ◽  
Sensitivity And Selectivity ◽  
Uniform Array

AbstractBiosensors based on liquid-gated carbon nanotubes field-effect transistors (LG-CNTFETs) have attracted considerable attention, as they offer high sensitivity and selectivity; quick response and label-free detection. However, their practical applications are limited due to the numerous fabrication challenges including resist-based lithography, in which after the lithography process, the resist leaves trace level contaminations over the CNTs that affect the performance of the fabricated biosensors. Here, we report the realization of LG-CNTFET devices using silicon shadow mask-based chemical-free lithography process on a 3-in. silicon wafer, yielding 21 sensor chips. Each sensor chip consists of 3 × 3 array of LG-CNTFET devices. Field emission scanning electron microscope (FESEM) and Raman mapping confirm the isolation of devices within the array chip having 9 individual devices. A reference electrode (Ag/AgCl) is used to demonstrate the uniformity of sensing performances among the fabricated LG-CNTFET devices in an array using different KCl molar solutions. The average threshold voltage (Vth) for all 9 devices varies from 0.46 to 0.19 V for 0.1 mM to 1 M KCl concentration range. This developed chemical-free process of LG-CNTFET array fabrication is simple, inexpensive, rapid having a commercial scope and thus opens a new realm of scalable realization of various biosensors.

Schottky diodes based on 2D materials for environmental gas monitoring: a review on emerging trends, recent developments and future perspectives

2021 ◽  
Author(s):  
Minu Mathew ◽  
Chandra Sekhar Rout
Keyword(s):  
Gas Sensing ◽  
2D Materials ◽  
Schottky Diodes ◽  
High Sensitivity ◽  
Future Perspectives ◽  
Working Temperature ◽  
Emerging Trends ◽  
Gas Sensing Properties ◽  
Recent Developments ◽  
Sensitivity And Selectivity

This review details the fundamentals, working principles and recent developments of Schottky junctions based on 2D materials to emphasize their improved gas sensing properties including low working temperature, high sensitivity, and selectivity.

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