scholarly journals PSII-25 Detection of neurotransmitter-related molecules by untargeted metabolomic analysis of rumen fluid from monensin-treated temperate and tropically adapted beef cattle

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 376-377
Author(s):  
Catherine L Wellman ◽  
Madaline Rabalais ◽  
Cory Klemashevich ◽  
Anil C Somenahally ◽  
Charles R Long ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Rumen Fluid ◽  
Aminobutyric Acid ◽  
Metabolomic Analysis ◽  
Hydroxyindoleacetic Acid ◽  
Ruminal Microbiota ◽  
Ad Libitum ◽  
Brahman Steers ◽  
Multiple Molecules

Abstract The effect of an ionophore on the ruminal metabolome of temperate and tropically-adapted cattle was evaluated. For 21 d, five Angus, 5 Brahman, and 5 BrahmanXAngus F1 steers (264 kg BW) with 5 steers per pen within breedtype, were fed Tifton bermudagrass hay ad libitum, at the conclusion of which rumen fluid (100-to-250 mL) was collected and stored at -80°C (PRE). During a subsequent 21-d period, all steers were fed Tifton hay ad libitum plus a supplement (908 g corn, 217 g soybean meal, 60 g dried molasses) with monensin (220 mg·steer−1·d−1) individually, at the conclusion of which rumen fluid (100-to-250 mL) was collected and stored at -80°C (POST). Rumen fluid samples were processed for an untargeted analysis by LC/MS on Compound Discoverer v.3.1. Approximately 2,043 compounds were detected with 825 being annotated by ChemSpider and other databases, of which several were associated with four neurotransmitters; acetylcholine (ACh), dopamine, γ-aminobutyric acid (GABA), and serotonin. After monensin, several ACh-related molecules increased (P < 0.003) in Angus (POST:PRE ratio=7.906) but ACh decreased (P < 0.043) in the F1 (ratio= 0.391) steers. Dopamine and dopamine-related molecules decreased (P < 0.01) in Angus (ratio= 0.149) and F1 steers (ratio= 0.206) after monensin. No changes for rumen presence of ACh or dopamine occurred in Brahman steers after monensin. Multiple molecules related to GABA increased in Angus (P < 0.03) and Brahman (P < 0.02) steers. Serotonin-related molecules such as the metabolite 5-hydroxyindoleacetic acid (5-HIAA; ratio= 4.389) increased (P < 0.005) only in Brahman steers. The addition of monensin to the diet differentially altered the presence of neurotransmitter-related molecules in the rumen fluid of temperate and tropically adapted beef cattle. Further research regarding physiological roles of ruminal neurotransmitters and modulation by ionophores that affect ruminal microbiota is warranted. Support: USDA FAH

scholarly journals PSXI-10 Influence of monensin in the diet of temperate and tropically adapted cattle on the ruminal and fecal metabolome and microbiome

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 378-378
Author(s):  
Madeline M Rabalais ◽  
Anil C Somenahally ◽  
Catherine L Wellman ◽  
Charles R Long ◽  
Ronald D Randel
Keyword(s):  
Fatty Acids ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Copy Number ◽  
Rumen Fluid ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Fecal Samples ◽  
Period 2 ◽  
Ad Libitum

Abstract The effect of monensin on the ruminal and fecal metabolome and microbiome of temperate and tropically-adapted cattle (264±6 kg BW) consuming Tifton bermudagrass hay plus a supplement was evaluated. During Period 1, 10 Angus, 10 Brahman, and 10 BrahmanXAngus steers were fed Tifton bermudagrass hay ad libitum for a 21-d period with 5 steers per pen within breedtype at the conclusion of which rumen fluid (100-to-250 mL) and fecal samples were collected and stored at -80C. During Period 2 all steers were fed Tifton bermudagrass hay ad libitum plus 1.185 kg of supplement (908 g corn, 217 g soybean meal, 60 g dried molasses) with or without monensin (220 mg·steer−1·d−1) individually for 21 d. Rumen fluid and fecal samples were stored at -80C until DNA was extracted and total prokaryotes, methanogens and fungi determined by qPCR. Rumen and fecal samples were extracted to quantify short chain fatty acids (SCFA) and monensin content by GC and LCMS, respectively. After 21 d of consumption of bermudagrass hay, neither rumen nor fecal prokaryotic copy number, fungal copy number, nor methanogen copy number differed among breedtypes. The rumen, but not fecal, fungi:bacterial ratio was least for Angus (0.77) and similar between Brahman (1.31) and BrahmanXAngus (1.21) steers (P < 0.0032). Ruminal SCFA concentrations were similar among breeds as was the acetate:propionate ratio (Angus: 3.52; Brahman: 3.64; BrahmanXAngus: 3.43). Monensin was not detected in control rumen fluid but averaged 363±24 ng/mL for monensin steers. Monensin reduced the acetate:propionate ratio (P < 0.001) by 18, 25.1, and 18.4% for the Angus, Brahman, and BrahmanXAngus steers, respectively. Rumen prokaryotic copy number (P < 0.10), methanogen copy number (P < 0.035), and methanogen:prokaryote ratio (P < 0.001) were reduced within each breedtype by monensin. Addition of monensin reduced methanogen copy number and improved utilization of the diet in temperate and tropically-adapted beef cattle.

The effect of supplemental protein source on ammonia levels in rumen fluid and blood and intake of alfalfa silage by beef cattle

1992 ◽  
Vol 72 (4) ◽  
pp. 853-862 ◽  
Author(s):  
J. W. G. Nicholson ◽  
E. Charmley ◽  
R. S. Bush
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Feed Intake ◽  
Dry Matter ◽  
Rumen Fluid ◽  
The Other ◽  
Feed Conversion ◽  
Eating Pattern ◽  
Physiological Limits ◽  
Alfalfa Silage

Growing beef cattle fed legume silage were supplemented with urea, soybean meal, fishmeal or a barley-based control in two experiments. In the first experiment, the concentrates were fed before the silage and in the second the silage and concentrates were mixed at feeding. In exp. 1 there were no significant (P > 0.05) effects of CP supplement on feed intake, weight gains or feed efficiency, although animals fed fishmeal gained the most and those fed urea the least. Rumen fluid ammonia N (RFNH3-N) increased more after feeding urea and soybean meal than the control or fishmeal supplements. Blood urea N (BUN) levels changed in parallel with RFNH3-N levels but the magnitude of change was reduced to about half. Blood ammonia N (BNH3-N) levels showed statistically significant changes only with the urea supplement. Cattle fed urea took longer to consume their supplement than those fed the other supplements. In exp. 2 there was no effect of supplement on gains or feed conversion. Animals fed the control supplement consumed more dry matter than those fed fishmeal (P < 0.05). Cattle fed the control supplement had lower RFNH3-N and BUN than those fed the other supplements (P < 0.01) but differences among the sources of CP were small. The urea supplement resulted in a higher BNH3-N than the control or fishmeal supplements (P < 0.05). There was no apparent effect of treatment on blood glucose level in either experiment. It was concluded that cattle adjust their daily eating pattern to maintain BNH3-N levels within physiological limits; that intake of silage dry matter is limited by level of non-protein N and rumen degradable protein; and that provision of bypass protein can improve efficiency of feed conversion of high-silage diets even when depressing feed intake. Key words: Alfalfa, silage, protein supplement, nitrogen, intake

scholarly journals 117 Functionality of a novel consensus bacterial 6-phytase variant in enhancing phosphorus digestibility in gestating and lactating sows

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 109-109
Author(s):  
Deepak Velayudhan ◽  
Xandra Benthem de Grave ◽  
Katie Waller ◽  
Leon Marchal ◽  
Yueming Dersjant-Li
Keyword(s):  
Soybean Meal ◽  
Random Effect ◽  
Positive Control ◽  
Negative Control ◽  
Combined Effect ◽  
Restricted Feeding ◽  
Sunflower Meal ◽  
Tukey Test ◽  
Lactating Sows ◽  
Ad Libitum

Abstract Two experiments were conducted to determine the efficacy of phytase on apparent total tract digestibility (ATTD) of phosphorus (P) in gestating and lactating sows. In Exp. 1, a total of 40 sows (parity 2–6) at d 78 of gestation were randomly assigned, based on parity, over 4 experimental diets. In Exp. 2, 40 sows (parity 2.0–6.0) at d 4 of lactation were randomly assigned based on parity, over 4 experimental diets. Both studies had a positive control (PC) diet with adequate levels of calcium (Ca) and digestible P (6.5 and 2.5 g/kg and 7.0 and 3.2 g/kg for gestation and lactation diets, respectively), a negative control (NC) diet with low Ca and digestible P levels (5.0 and 1.0 g/kg and 5.0 and 1.5 g/kg for gestation and lactation diets, respectively), and NC supplemented with a novel consensus bacterial 6-phytase variant (PhyG) or a commercial Buttiauxella sp. phytase (PhyB), both at 500 FTU/kg. Diets were corn, soybean meal and sunflower meal based (phytate P of 3.0 g/kg) with restricted feeding during gestation and ad libitum during lactation. In both studies, fecal samples were collected after an adaptation of 14 d on 4 successive days to determine ATTD of P. Data were analyzed using ANOVA, treatment mean comparison using Tukey test using JMP 14. In addition, data from the Exp. 1 and 2 were pooled to further investigate their combined effect, using trial as random effect. In both studies, supplementation of both phytases improved (P &lt; 0.05) ATTD of P vs NC. With data combined from two trials, sows fed PhyG showed greater (P &lt; 0.05) ATTD of P when compared those fed PhyB. In conclusion, PhyG at 500 FTU/kg showed a greater ATTD of P in sows when compared to the PhyB at 500 FTU/kg, when data combined from two trials.

scholarly journals PSVI-22 Rumen microbiome of beef cattle is modulated by backgrounding systems

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 220-220
Author(s):  
Bobwealth O Omontese ◽  
Ashok K Sharma ◽  
Jason Langlie ◽  
Joe Armstrong ◽  
Alfredo DiCostanzo ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Developmental Stages ◽  
Production Systems ◽  
Rumen Fluid ◽  
Illumina Miseq ◽  
High Energy ◽  
Shannon Index ◽  
Bacterial Gene ◽  
Beef Calves ◽  
Rumen Microbiome

Abstract Backgrounding (BKG) segment in beef production systems is characterized by utilization of different forages which affect growth performance and carcass characteristics. However, it is unclear how BKG systems impact rumen microbiome. We investigated rumen microbiome dynamics of beef calves under different BKG systems. At weaning, Angus and Angus x Simmental beef calves (n = 38) were stratified by age, weight, and sex in a completely randomized design into 1 of 3 BKG treatments for 55 d: 1) perennial pasture (PP; quackgrass, orchardgrass; smooth bromegrass, red clover, and alfalfa); 2) summer annual cover crop (CC; cereal oats, purple top turnips, hunter forage brassica, and graza forage radish); and 3) dry lot (DL; haylage, corn, and DDGS). After BKG, all calves were assigned to a high energy ration in a feedlot. Rumen sample was collected via esophageal tubing at weaning, BKG and feedlot. A total of 190 rumen fluid samples were used to sequence the hypervariable V4 region of the 16S rRNA bacterial gene on an Illumina MiSeq platform. The results showed that BKG systems largely influenced rumen bacterial communities. Specifically, microbiome composition and diversity were not different at weaning, diverged significantly during BKG (Shannon index, Bray Curtis distance metrics; P &lt; 0.001) and homogenized during feedlot. During the BKG segment, the bacterial genera Agrobacterium, Coprococcus, and Ruminococcus were dominant in CC whereas Fibrobacteraceae and Mycoplasmataceae was most dominant in DL. Moreover, rumen microbiome patterns of CC and DL calves showed increased plasticity in early stages of development but not during feedlot with PP showing fewer changes over time. These results indicate that BKG systems significantly modulate the rumen microbiome of beef cattle and, underscore the importance of early developmental stages as potential targets for feeding interventions that can impact the animal microbiome to enhance animal performance.

PRELIMINARY EVALUATION OF THE ADDITION OF GLUCOGENIC MATERIALS TO THE RATIONS OF LACTATING COWS

1971 ◽  
Vol 51 (3) ◽  
pp. 721-727 ◽  
Author(s):  
L. J. FISHER ◽  
J. D. ERFLE ◽  
F. D. SAUER
Keyword(s):  
Fatty Acids ◽  
Propylene Glycol ◽  
Milk Fat ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Preliminary Evaluation ◽  
Early Lactation ◽  
Lactating Cows ◽  
Ad Libitum ◽  
Beta Hydroxybutyrate

Glutamate, succinate, propylene glycol, or glycerol were added to a basic concentrate at 3.3% of air-dry feed. Each concentrate was fed both ad libitum and in restricted amounts to four cows in early lactation. Dietary intake, milk yield and composition, molar proportions of rumen volatile fatty acids and blood glucose, ketones and plasma free fatty acids were used as criteria of effect of these supplements. Propylene glycol in the diet resulted in a lower intake of concentrate compared with glycerol (11.44 versus 14.30 kg/day) and significantly decreased (P < 0.05) rumen butyrate and plasma beta-hydroxybutyrate. Glutamate supplementation prevented the fall in milk fat content which occurred when the other three supplemented concentrates were fed ad libitum, and this effect may have been related to the constancy in the ratio of acetate to propionate in the rumen fluid.

NUTRITIONAL VALUE OF LOW GLUCOSINOLATE RAPESEED MEAL FOR SWINE

1975 ◽  
Vol 55 (1) ◽  
pp. 61-70 ◽  
Author(s):  
J. M. BELL
Keyword(s):  
Brassica Napus ◽  
Soybean Meal ◽  
Nutritional Value ◽  
Control Diet ◽  
Rapeseed Meal ◽  
Glucosinolate Content ◽  
Protein Digestion ◽  
Brassica Napus L ◽  
Ad Libitum

Five swine experiments were conducted to evaluate rapeseed meal (RSM) of low glucosinolate content (Brassica napus L. cv. Bronowski). Two experiments involved 0, 25, 50, 75 and 100% substitution of either Bronowski meal or regular (B. campestris) meal for soybean meal or fishmeal used in the control diet. One experiment compared ad libitum-fed and partially restricted pigs. Another experiment involved digestibility studies, and the final one involved methionine and lysine supplementation. As the dietary levels of either Bronowski or regular RSM increased in the ration, protein digestion coefficients decreased from 79 and 80% to 76 and 78%, respectively, and energy coefficients decreased from 82% to 79 and 78%, respectively. The protein and energy digestibility coefficients for Bronowski RSM were estimated to be 68 and 59%; for regular RSM, 65 and 54%. With barley–wheat–RSM diets, pigs responded to 0.1% methionine, but not to lysine (P > 0.05). Pigs fed ad libitum consumed more Bronowski than regular RSM diet and performed as well as pigs fed soybean meal diets.

Effect of forage maize harvested at different stages of maturity on feed intake and performance by growing beef cattle

1998 ◽  
Vol 1998 ◽  
pp. 148-148
Author(s):  
E.M. Browne ◽  
M.J. Bryant ◽  
D.E. Beever ◽  
C.L. Thorp
Keyword(s):  
Statistical Analysis ◽  
Beef Cattle ◽  
Dry Matter ◽  
Maize Silage ◽  
Initial Weight ◽  
Forage Maize ◽  
Maize Variety ◽  
Ad Libitum ◽  
Pre Treatment ◽  
And Performance

Dry matter (DM) concentration of maize silage is directly related to maturity of the crop at harvest and widely reported to be positively correlated with total forage DM intake. The objective of this experiment was to investigate these effects using a late maturing beef genotype and a contemporary forage maize variety.Forage maize (variety Hudson) was harvested at four different stages of maturity during September and October 1996. Each stage of maturity was ensiled in a separate clamp with no additive. Resultant silage corrected dry matter contents were 247 (L), 305 (M/L), 331(M/H) and 388 (H)g/kgFW, respectively. Each diet was formulated to be isonitrogenous with fishmeal fed twice daily on top of the silage. Silage was offered ad libitum to 32 growing Simmental X Friesian heifers (mean initial weight 217kg), housed in individual pens in an open-sided Dutch barn and bedded on wheat straw. Eight animals were allocated to each treatment, in a completely randomised design with pre-treatment intake (non-experimental maize silage) used as a covariate in the statistical analysis.

Dietary gamma-aminobutyric acid ameliorates growth impairment and intestine dysfunction in turbot (Scophthalmus maximus L.) fed a high soybean meal diet.

2021 ◽  
Author(s):  
Chaoqun Li ◽  
Yuan Tian ◽  
Qinyuan Ma ◽  
Beili Zhang
Keyword(s):  
Growth Performance ◽  
Soybean Meal ◽  
Scophthalmus Maximus ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Growth Impairment ◽  
Turbot Scophthalmus Maximus

Over-substitution of fishmeal with soybean meal (SBM) commonly leads to inferior growth performance and intestinal dysfunction in fish. This study aims to evaluate whether dietary gamma-aminobutyric acid (GABA) could ameliorate...

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