scholarly journals PSXII-19 Urine Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers during the Growing and Finishing Period on Forage- and Concentrate- Based Diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 442-442
Author(s):  
Harvey C Freetly ◽  
Virginia Artegoitia ◽  
John Newman ◽  
Ron Lewis ◽  
Andrew P Foote
Keyword(s):  
Bile Acids ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Average Daily Gain ◽  
Carcass Composition ◽  
Carcass Weight ◽  
Cattle Feed ◽  
Negatively Associated ◽  
Stage 1 ◽  
Urine Metabolomics

Abstract A discovery project to identify non-invasive biomarkers that can detect subtle metabolic discrepancies for cattle feed efficiency was performed using untargeted and targeted urine metabolomics by ultra-performance liquid chromatography-mass spectrometry. Individual feed intake and body weight gain were measured in crossbred steers (n = 80) on a forage-based growing ration (stage-1) followed by a high-concentrate diet finishing ration (stage-2). Urine was collected on study days 0, 21, 42, 63, and 83 for each dietary stage. In total, 28 steers with the greatest and the least average daily-gain (ADG) within 0.32 SD of the mean of dry-matter-intake (DMI) were used. A principal component analysis of the untargeted metabolites fully segregated the highest-ADG and lowest-ADG animals, with overlap across diets (both stages). The urinary untargeted metabolites that segregated the ADG-groups (n = 199; P < 0.05), included steroid-hormones, bile-acids, alpha-linolenic acid metabolites, vitamin-B6, along with products of glycine, serine and threonine metabolism (metabolic pathway analysis: impact-value > 0.50; FDR < 0.10). Bile acids and steroids were then quantified in urine and their associations with animal performance and carcass composition evaluated by correlation and multiple logistic regression AUC-ROC curve analyses. In stage-1, urine concentration of cortisone was associated (P < 0.05) with ADG (r = -0.28), DMI (r = -0.40) and ribeye-area (r = -0.28); cortisol was associated with DMI (r = -0.32; P < 0.01) and testosterone was associated with ADG (r = -0.28; P < 0.01). The urine concentrations of 18 measured bile acids were negatively associated (P < 0.05) with DMI, and secondary bile acids were negatively associated (P < 0.01) with marbling and hot-carcass-weight. In stage-2, negative association between the bile acids glycocholic acid and deoxycholic acid with marbling and hot-carcass-weight were identified. Urine metabolomics provide new insight into the physiological mechanisms and potential biomarkers of cattle feed efficiency. USDA is an equal opportunity provider and employer.

scholarly journals Non-invasive metabolomics biomarkers of production efficiency and beef carcass quality traits

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Virginia M. Artegoitia ◽  
J. W. Newman ◽  
A. P. Foote ◽  
S. D. Shackelford ◽  
D. A. King ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Production Efficiency ◽  
Average Daily Gain ◽  
Stepwise Logistic Regression ◽  
Carcass Quality ◽  
Physiological Mechanisms ◽  
Cattle Feed ◽  
Non Invasive ◽  
Fat Thickness ◽  
Stage 1

AbstractThe inter-cattle growth variations stem from the interaction of many metabolic processes making animal selection difficult. We hypothesized that growth could be predicted using metabolomics. Urinary biomarkers of cattle feed efficiency were explored using mass spectrometry-based untargeted and targeted metabolomics. Feed intake and weight-gain was measured in steers (n = 75) on forage-based growing rations (stage-1, 84 days) followed by high-concentrate finishing rations (stage-2, 84 days). Urine from days 0, 21, 42, 63, and 83 in each stage were analyzed from steers with the greater (n = 14) and least (n = 14) average-daily-gain (ADG) and comparable dry-matter-intake (DMI; within 0.32 SD of the mean). Steers were slaughtered after stage-2. Adjusted fat-thickness and carcass-yield-grade increased in greater-ADG-cattle selected in stage-1, but carcass traits did not differ between ADG-selected in stage-2. Overall 85 untargeted metabolites segregated greater- and least-ADG animals, with overlap across diets (both stages) and breed type, despite sampling time effects. Total 18-bile acids (BAs) and 5-steroids were quantified and associated with performance and carcass quality across ADG-classification depending on the stage. Stepwise logistic regression of urinary BA and steroids had > 90% accuracy identifying efficient-ADG-steers. Urine metabolomics provides new insight into the physiological mechanisms and potential biomarkers for feed efficiency.

scholarly journals Non-Invasive Metabolomics Biomarkers of Production Efficiency and Beef Carcass Quality Traits

2021 ◽  
Author(s):  
Virginia M. Artegoitia ◽  
J. W. Newman ◽  
A. P. Foote ◽  
S. D. Shackelford ◽  
D.A. King ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Production Efficiency ◽  
Average Daily Gain ◽  
Stepwise Logistic Regression ◽  
Carcass Quality ◽  
Physiological Mechanisms ◽  
Cattle Feed ◽  
Non Invasive ◽  
Fat Thickness ◽  
Stage 1

Abstract The inter-cattle growth variations stem from the interaction of many metabolic processes making animal selection difficult. We hypothesized that growth could be predicted using metabolomics. Urinary biomarkers of cattle feed efficiency were explored using mass spectrometry-based untargeted and targeted metabolomics. Feed intake and weight-gain was measured in steers (n=75) on forage-based growing rations (stage-1, 84 d) followed by high-concentrate finishing rations (stage-2, 84 d). Urine from days 0, 21, 42, 63, and 83 in each stage were analyzed from steers with the greater (n = 14) and least (n = 14) average-daily-gain (ADG) and comparable dry-matter-intake (DMI; within 0.32 SD of the mean). Steers were slaughtered after stage-2. Adjusted fat-thickness and carcass-yield-grade increased in greater-ADG-cattle selected in stage-1, but carcass traits did not differ between ADG-selected in stage-2. Overall 85 untargeted metabolites segregated greater- and least-ADG animals, with overlap across diets (both stages) and breed type, despite sampling time effects. Total 18-BAs and 5-steroids were quantified and associated with performance and carcass quality across ADG-classification depending on the stage. Stepwise logistic regression of urinary BA and steroids had >90% accuracy identifying efficient-ADG-steers. Urine metabolomics provides new insight into the physiological mechanisms and potential biomarkers for feed efficiency.

Post-weaning growth rate effects on body composition of Nellore bulls

2020 ◽  
Vol 60 (6) ◽  
pp. 852
Author(s):  
Luiz H. P. Silva ◽  
Pedro V. R. Paulino ◽  
Pedro D. B. Benedeti ◽  
Mauricio M. Estrada ◽  
Lyvian C. Alves ◽  
...  
Keyword(s):  
Growth Rate ◽  
Body Composition ◽  
Feed Efficiency ◽  
Compensatory Growth ◽  
Average Daily Gain ◽  
High Growth ◽  
High Growth Rate ◽  
Carcass Weight ◽  
Apparent Digestibility ◽  
Rate Effects

Context Previously feed-restricted cattle may exhibit compensatory growth during the finishing phase. However, the efficiency in converting feed into carcass should be evaluated since cattle undergoing compensatory growth usually have high non-carcass weight gain. Aims The objective of this study was to evaluate the effect of growth rate throughout the post-weaning growing phase on subsequent feed efficiency, carcass gain, and gain composition. Methods Thirty-nine weaned young Nellore bulls averaging 230.4 ± 5.62 kg of bodyweight and 8.5 ± 0.25 months of age were used. Initially, five bulls were slaughtered as a reference initial group. The remaining bulls were randomly assigned to one of three nutritional plans to achieve Low (0 kg/day), Medium (0.6 kg/day) or High (1.2 kg/day) average daily gain (ADG) throughout the post-weaning growing phase, followed by high growth rate during the finishing phase. One-half of the bulls from each treatment were slaughtered at the end of the post-weaning growing phase, and the other one-half after the finishing phase. During both phases the feed intake, apparent digestibility, performance, and body composition were evaluated. Key results Throughout the post-weaning growing phase, High bulls had greater ADG and more efficiently converted feed into carcass, compared with other nutritional plans (P < 0.01). Throughout the finishing phase, Low bulls had greater ADG, carcass gain, and feed efficiency than High and Medium bulls (P < 0.01). Previous feed restriction did not affect (P > 0.05) apparent digestibility. During the finishing phase, previously restricted bulls fully compensated for the lost visceral organ weight, whereas the losses of bodyweight and carcass weight were only partially compensated. Throughout finishing, Low bulls had the greatest feed efficiency and profitability among nutritional plans. However, considering the overall experiment, Hight bulls converted feed into carcass more efficiently than Low bulls (P = 0.02), but did not differ from Medium (P > 0.05). Conclusions Although previously restricted bulls had greater performance and efficiency throughout finishing, the improvement was not enough to reach the same carcass weight at the same age of the unrestricted bulls. Implications Despite the greater profitability of previously restricted bulls throughout finishing, unrestricted bulls were more profitable considering both growing and finishing phases.

scholarly journals Influence of dietary roughage level and Megasphaera elsdenii on feedlot performance and carcass composition of thin cull beef cows fed for a lean market

2019 ◽  
Vol 4 (1) ◽  
pp. 159-169
Author(s):  
Jonathan C DeClerck ◽  
Nathan R Reeves ◽  
Mark F Miller ◽  
Bradley J Johnson ◽  
Gary A Ducharme ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Body Condition Score ◽  
Average Daily Gain ◽  
Beef Cows ◽  
Carcass Composition ◽  
Finishing Diets ◽  
Caloric Density ◽  
Significant Difference ◽  
Megasphaera Elsdenii ◽  
Compensatory Gain

Abstract One hundred forty-four cull cows (body condition score = 2.10 ± 0.61; BW = 456 ± 47 kg) were organized into a 2 × 2 factorial design (48 pens, 12 pens/treatment, and 3 cows/pen) to evaluate the effect of dietary roughage level and oral drenching of Megasphaera elsdenii NCIMB 41125 (M. elsdenii culture; Lactipro Advance; MS Biotec Inc., Wamego, KS) on performance and carcass characteristics. Cattle were finished over a 42-day realimentation period, and aggressively stepped up over a 10-day period to either a high roughage finisher (HRF; 25% roughage) or a low roughage finisher (LRF; 10% roughage). Within diet, cattle were administered no probiotic or 100 mL of M. elsdenii culture (M. elsdenii NCIMB 41125, 2 108 cfu/mL) on day 0. No diet × probiotic interactions were detected (P ≥ 0.15), suggesting that the magnitude of the response was not influenced by the concentrate level of the diet. The main effect of diet triggered several significant responses. Decreasing roughage level tended to improve average daily gain (ADG) by 9.7% (0.26 kg, P = 0.08), while decreasing dry matter intake (DMI) by 0.9 kg (P = 0.09), provoking a 19.7% enhancement of feed efficiency (0.036 units, P < 0.01). However, interim data revealed declines of performance parameters among both diets with a significant difference between treatments only documented during the final phase of the realimentation period. During the final 14 days, LRF posted a 0.68 kg increase in ADG (P = 0.05) and a 2.0 kg decrease in DMI (P = 0.01), translating to improved feed efficiency (0.054 units, P = 0.03). This suggests that increasing the caloric density of finishing diets may help offset the regression of performance typically observed following a compensatory gain. No carcass traits were impacted by either diet or M. elsdenii culture (P ≥ 0.08). Overall, oral drenching of M. elsdenii culture tended to augment ADG (0.26 kg, P = 0.08) and carcass ADG (0.20 kg, P = 0.10). Implying that M. elsdenii culture was effective at alleviating the acidosis risk prompted by the rapid step-up period employed in the trial and may help capitalize on the narrow timeline of compensatory gain in cull cow realimentation.

scholarly journals Effects of feeding transgenic canola on apparent digestibility, growth performance and carcass characteristics of lambs

2003 ◽  
Vol 83 (2) ◽  
pp. 299-305 ◽  
Author(s):  
K. Stanford ◽  
J. L. Aalhus ◽  
M. E. R. Dugan ◽  
G. L. Wallins ◽  
R. Sharma ◽  
...  
Keyword(s):  
Growth Performance ◽  
Meat Quality ◽  
Feed Efficiency ◽  
Transgenic Line ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Carcass Composition ◽  
Canola Meal ◽  
Apparent Digestibility ◽  
Transgenic Canola

This study evaluated the effects of including meal from glyphosate-tolerant (Roundup-Ready®) canola (RRC) in barley-based diets for lambs on apparent digestibility of the diets, growth performance of the lambs, and carcass quality and composition. Four isonitrogenous diets were prepared that included canola meal [6.5%, dry matter (DM) basis] from four different sources (two commercially available canola blends, COM1 and COM2; a transgenic line, RRC and the parental non-transgenic line from which RRC was derived, PAR). Apparent digestibilities of the four diets were determined using eight mature wethers (67.8 ± 2.3 kg) in a replicated Latin square with four 21-d periods. No aspect of digestibility (DM, fibre, or nitrogen balance) was influenced by canola source. The growth trial involved 60 early-weaned Arcott lambs (30 ewes; 30 wethers; initial age approximately 2 mo; initial weight 21.5 ± 1.0 kg). The lambs were blocked by weight and gender for assignment to treatments, and fed the diets until reaching or exceeding 45 kg body weight. Intake of DM was similar among lambs fed COM1, COM2 and PAR diets, and between PAR and RRC (COM1, COM2 > RRC, P < 0.05). Diet did not affect (P > 0.05) average daily gain or feed efficiency. Carcass yield grade was higher (P < 0.05) for COM1 and COM2 diets than for PAR or RRC, although carcass composition did not differ (P > 0.05) between PAR and RRC. Canola source did not affect (P > 0.05) meat tenderness, as determined by shear force, drip loss or intramuscular fat content. Meat colour o f RRC-fed lambs did not differ from that of all other treatment groups. In this study, including canola meal prepared from glyphosate-tolerant canola did not alter diet digestibility, feed efficiency, growth performance, carcass characteristics or meat quality of lambs. Key words: Growth, lambs, meat quality, ruminant, transgenic canola

Finishing steers on high-grain rations. Effects of type of silage, level of urea, vitamin A and cobalt supplementation on body-weight gain, feed efficiency and carcass composition

1967 ◽  
Vol 68 (1) ◽  
pp. 1-9 ◽  
Author(s):  
J. G. Morris ◽  
R. J. W. Gartner
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Vitamin A ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Grain Sorghum ◽  
Carcass Composition ◽  
Finishing Steers ◽  
Sorghum Silage ◽  
Sorghum Grain

1. A 23 factorial with a split-plot allocation of treatments was used to investigate the effects of silage type, (sweet v. grain sorghum); level of urea, (60ν. 120 g. per head per day); vitamin A,(0ν. 40,000 i.u. per head per day), and intraruminal cobalt oxide pellet on the performance of steers fed rations of 90% sorghum grain, 10% sorghum silage.

scholarly journals Effect of feeding dry-rolled corn or whole shelled corn during the finishing phase on growth performance and carcass characteristics

2020 ◽  
Author(s):  
T B Freitas ◽  
T L Felix ◽  
C Clark ◽  
F L Fluharty ◽  
A E Relling
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Dry Matter ◽  
Block Design ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Feedlot Cattle ◽  
Carcass Weight ◽  
Muscle Area ◽  
Quality Grade

Abstract The objectives of this trial were to evaluate the effect of corn processing during the finishing phase on feedlot cattle performance and carcass characteristics. We hypothesized that steers fed dry-rolled corn would have better feed efficiency (less feed necessary to the same amount of gain) than steers fed whole shelled corn. Ninety-five backgrounded Angus-cross cattle, (initial body weight (BW) = 263 ± 9.8 kg) were used in a randomized complete block design in a feedlot setting. Cattle were divided in to 3 blocks: heifers (n = 31, 4 pens; initial BW = 267 ± 1.3 kg), light steers (n = 32, 4 pens; initial BW = 253 ± 1.3 kg), and heavy steers (n = 32, 4 pens; initial BW = 279 ± 1.4 kg). Diets contained 70% corn (experimental treatment), 15% dried distiller’s grains with solubles, 7% grass hay, and 8% supplement, on a dry matter basis. Cattle were fed for 217, 224, and 231 ± 8 d for the heifer, heavy steer, and light steer blocks, respectively. Two pens within each block were randomly assigned to one of the following treatments: 1) dry-rolled corn (DRC) or 2) whole shelled corn (WSC). Animal growth performance and carcass characteristics were analyzed using the MIXED procedure of SAS, including the fixed effect of treatment. Pen and block were included as random effects. Carcass characteristics USDA Yield Grade and Quality Grade distributions were compared using the GLIMMIX procedure of using the same model than the growth performance data. Data are presented as LSMeans. Cattle fed DRC had greater average daily gain (ADG; P = 0.02) and final BW (P &lt; 0.01) when compared with cattle fed WSC. Even though ADG was greater for the DRC treatment, feed efficiency was similar (P = 0.45) because dry matter intake (DMI) was also greater (P = 0.04) for DRC when compared with the WSC treatment. Cattle fed DRC also had greater hot carcass weight (P &lt; 0.01), dressing percentage (P &lt; 0.01), and Longissimus dorsi muscle area (LMA; P &lt; 0.01) than cattle fed WSC. No differences (P ≥ 0.18) were observed for marbling score, USDA Yield Grade or USDA Quality Grade. In conclusion, feeding DRC to feedlot cattle improved ADG, hot carcass weight, and LMA, without affecting feed efficiency or meat quality.

scholarly journals Growth evaluation of Oreochromis niloticus fed different concentrations of choline and methionine

2020 ◽  
Vol 5 (2) ◽  
pp. 122-128
Author(s):  
Rudy Agung Nugroho ◽  
Normala Sari ◽  
Retno Aryani ◽  
Hetty Manurung ◽  
Rudianto Rudianto
Keyword(s):  
Oreochromis Niloticus ◽  
Feed Efficiency ◽  
Crude Protein ◽  
Growth Parameters ◽  
Body Weight Gain ◽  
Average Daily Gain ◽  
Feed Conversion ◽  
Tilapia Diet ◽  
And Control ◽  
Feed Conversion Rate

A 12-week research was conducted to determine the effects of choline and methionine supplementation on the growth performance of Nile tilapia (Oreochromis niloticus). Three diets, P2-P4 with 0.04; 0.08; 0.12 % of choline; P6-P8 with same levels choline plus methionine (0.15 %); a diet with only 0.15 % methionine (P5); and control without any supplementation (P1) were fed to tilapia (initial weight ±21.05 g) for 12 weeks. Final weight (FW), Body Weight Gain (BWG), Average Daily Gain (ADG), Specific Growth Rate (SGR), and Protein Efficiency Ratio (PER), Feed Efficiency (FE), Feed Intake (FI), Feed Conversion Rate (FCR), Survival Rate (SR), crude protein and lipid of fish fed various levels of choline and methionine were also measured. The results showed that tilapia fed 0.04 % choline (P2) had significantly better growth parameters and feed efficiency than other groups. Meanwhile, SR of tilapia was not affected by any supplementation of choline and methionine. The tilapia fed 0.08% choline (P3) showed the highest crude protein (52.50 ± 0.98 %) in the carcass proximate but low lipid (19.03 ± 0.10 %). This finding demonstrated the benefits of choline (0.04-0.08 %) supplementation in the tilapia diet in term of growth and carcass proximate.

scholarly journals Effects of distillated myrtle (Myrtus communis L.) leaves’ intake on cull ewes’ body weight gain, carcass composition and meat quality

2020 ◽  
Vol 18 (4) ◽  
pp. e0613
Author(s):  
Souha Tibaoui ◽  
Hadhami Hajji ◽  
Samir Smeti ◽  
Ilyes Mekki ◽  
Ines Essid ◽  
...  
Keyword(s):  
Body Weight ◽  
Meat Quality ◽  
Body Weight Gain ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Dietary Treatment ◽  
Carcass Composition ◽  
Partial Substitution ◽  
Control Group ◽  
Feed Conversion

Aim of study: Cull ewes are characterized by poor body condition, low body weights and tough meat texture. This work aimed to investigate the effect of distillated myrtle leaves (MDL) intake on body weight (BW) gain; carcass characteristics and meat quality of Barbarine cull ewes.Area of study: Northwest of TunisiaMaterial and methods: 27 Barbarine ewes were assigned into 3 groups receiving 500 g of oat hay and 750 g of concentrate control group (C), while they were given concentrate and pellets, containing 87% MDL, as substitute to hay in MHay group; for MConc group, they were fed hay, concentrate and pellets containing 30% MDL in partial substitution to concentrate. At the end of the fattening period (90 days), ewes were slaughtered.Main results: The dry matter intake was higher (p<0.05) for MConc and C groups. The average daily gain was significantly higher for C and MConc than MHay groups (113 and 107 vs. 87 g, respectively). Ewes fed MHay and MConc had a significantly lower feed conversion rate than Control group (12.5 vs. 15.4). Dietary treatment had no significant effect on carcass joint’s weight and proportions. The dressing percentage and carcass tissue composition were similar for all groups. The ultimate pH, water cooking loss and color parameters values were unaffected by the type of diet.Research highlights: These findings revealed that MDL could substitute, in ewes feeding, up to 87% to hay or up to 30% to concentrate without negative effects on body weight, carcass characteristics and meat quality.

scholarly journals Influence of Crossbreeding of Ghezel Ram With Grey Shirazi Ewe on Growth Performance, Feed Efficiency and Carcass Traits of Fattening Lambs

2021 ◽  
Author(s):  
Abdolhamid Karimi ◽  
Mohammad Javad Abarghuei ◽  
Alidad Boostani
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Average Daily Gain ◽  
Carcass Traits ◽  
Live Weight ◽  
Carcass Weight ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Group 12

Abstract In this investigation, performance, feed efficiency and carcass traits of 48 weaned crossbred Grey Shirazi × Ghezel and purbred Grey Shirazi fattening lambs (mean live weight, 28.5 ± 0.5 kg and age, 115 ± 5 days) were evaluated. Lambs were divided into 4 groups contain the GM group (12 purebred male lambs of Grey Shirazi ewe × Grey Shirazi ram), the GF group (12 purebred female lambs of Grey Shirazi ewe × Grey Shirazi ram), the CM group (12 crossbred male lambs of Grey Shirazi ewe × Ghezel ram) and the CF group (12 crossbred female lambs of Grey Shirazi ewe × Ghezel ram). The dry matter intakes (DMI), average daily gain (ADG) and carcass traits in the lambs were determined. The DMI was greater in CF lambs compared to GF lambs (P < 0.001). Feed conversion ratio (FCR) was higher in male lambs comparing to female lambs (P = 0.001). The final body weight, ADG, live weight at the slaughtering and hot carcass weight (HCW) were lower in GF lambs compared to another lambs (P < 0.001, P = 0.003). Cold carcass weight (CCW) in CM lambs was higher than in other groups. The Longissimus dorsi (LD) area was higher in crossbreed male lambs (P = 0.001). Crossbreeding improved grow performance (final body weight and ADG) in male and female lambs. Carcass trait was improved by crossbreeding especially in male lambs. Sex effect on growth performance and carcass traits was noticeable and male lambs had superior growth.

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