PSII-14 Supplementation of Bacillus Amyloquefaciens on Systemic Immunity of Weaned Pigs Experimentally Infected with a Pathogenic E. coli

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 168-169
Author(s):  
Cynthia Jinno ◽  
Braden T Wong ◽  
Martina Kluenemann ◽  
Xunde Li ◽  
Yanhong Liu
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Neutrophil Count ◽  
Complete Blood Count ◽  
Control Group ◽  
Blood Cell Count ◽  
Weaned Pigs ◽  
E Coli ◽  
Antibiotic Group ◽  
Wbc Count

Abstract The objective of this experiment was to investigate the effects of dietary supplementation of Bacillus amyloquefaciens on total and differential blood cell count in weaned pigs experimentally infected with a pathogenic E. coli. A total of 50 weaned pigs (7.41 ± 1.35 kg) were individually housed in disease containment rooms and randomly assigned to one of the 5 treatments: sham control (CON-), sham B. amyloquefaciens (BAM-), challenged control (CON+), challenged B. amyloquefaciens (BAM+) and challenged carbadox (CAR+). The experiment lasted 28 days with 7 days’ adaptation and 21 days after the first E. coli inoculation. The doses of F18 E. coli inoculum were 1010 CFU/3 mL oral dose daily for 3 consecutive days. Whole blood samples were collected from all pigs on d -7, and d 0, 7, 14, and 21 post infection (PI) to measure total and differential blood cell count by complete blood count (CBC) analysis. Supplementation of BAM or CAR increased (P < 0.05) either the percentage or the number of lymphocytes on d 0 before E. coli inoculation. E. coli challenge increased (P < 0.05) white blood cell (WBC) count on d 7 and 21 PI, while supplementation of BAM tended (P < 0.10) to have low WBC on d 7 PI and had lower (P < 0.05) WBC on d 21 PI compared with CON+. Pigs in BAM+ also had lower (P < 0.05) neutrophil count on d 14 PI, pigs fed with CAR had lower (P < 0.05) neutrophil count on d 14 and 21 PI, compared with pigs in CON+. No difference was observed in red blood cell profile among all treatments throughout the experiment. In conclusion, pigs fed with B. amyloquefaciens have similar systemic immune response to pigs in antibiotic group and have relatively lower systemic inflammation caused by E. coli compared with control group.

Influence of increased doses of fenbendazole supramolecular complex on sheep’s organism

2018 ◽  
Vol 12 (2) ◽  
pp. 46-52 ◽  
Author(s):  
A.I. Varlamova
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Biochemical Parameters ◽  
Negative Influence ◽  
Supramolecular Complex ◽  
Control Group ◽  
Blood Cell Count ◽  
Russian Scientific ◽  
Russian Scientific Research Institute ◽  
Hematological And Biochemical Parameters

The purpose of the research: study of the influence of increased doses of fenbendazole supramolecular complex (FSMC) on sheep’s organism. Materials and methods. The experiment was carried out at the Podolsk Department of All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin on 20 manorial invasion-free sheep aged 2-3 years old. Animals were divided according to the principle of analogues into 4 groups, 5 heads in each group. Animals of the 1, 2 and 3 group were orally administered with FSMC given as a single dose of 2, 6, 10 mg/kg, respectively, according to the active substance, i.e in therapeutic and in a dose increased by 3 and 5 times. Sheep of the fourth group didn’t receive the drug and they were as control. Study of clinical, hematological and biochemical parameters of animals from all groups was conducted 1 day before and in 1, 3, 5 days after administration of the drug by means of standard methods. Results and discussion. FSMC in therapeutic dose as well as in a dose increased by 3 and 5 times doesn’t have negative influence on clinical, hematological and biochemical parameters of the sheep. State of the sheep, which received the drug in doses of 20, 60, 100 mg/kg, was within the physiologically normal state and didn’t differ from the state before administration of the drug and from the animals of the control group. Drug security index exceeds 5. Red blood cell count, white blood cell count, hemoglobin count, leukogram parameters as well as biochemical parameters of blood: activity of alkaline phosphatase and amylase, bilirubin, creatinine, urea and glucose counts were within normal limits and didn’t differ from the parameters of the control animals.

scholarly journals Predictive values of Alvarado score, serum C-reactive protein, and white blood cell count in the diagnosis of acute appendicitis: a prospective study

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Ademola Olusegun Talabi ◽  
Tewogbade Adeoye Adedeji ◽  
Oludayo Adedapo Sowande ◽  
Olusanya Adejuyigbe
Keyword(s):  
Acute Appendicitis ◽  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell Count ◽  
White Blood Cell ◽  
C Reactive Protein ◽  
Blood Cell Count ◽  
Alvarado Score ◽  
Reactive Protein ◽  
Wbc Count

Abstract Background The diagnosis of acute appendicitis in children is quite challenging as the rate of negative appendectomy varies between 15 and 57%. Increased utilization of imaging diagnostic facilities in advanced countries seems to have reduced the incidence of operating on normal appendix to a single digit. In low- and middle-income countries, the incidence remains unacceptably high (double digits). Inflammatory markers and scoring systems may be a suitable adjunct to increase diagnostic yield in most third world countries. Thus, the aim of this study was to evaluate the diagnostic value of Alvarado score, white blood cell count, and serum C-reactive protein in children with acute appendicitis. Results The ages of patients ranged between 4 and 15 years with a mean of 11.2 ± 2.8 years. The male to female ratio was 1.4 to 1.0. Nineteen percent of patients had negative appendiceal findings on histological examination. The sensitivity and specificity of Alvarado score, C-reactive protein estimation, total white blood cell count in diagnosing acute appendicitis were 86.4% and 63.2%, 98.8% and 36.8%, and 51.9% and 89.5% respectively. Alvarado score has the highest area under ROC curve analysis 0.824, 95% CI of 0.724 to 0.924 compared with CRP, 0.769. 95% CI of = 0.647 to 0.891 and WBC count, 0.765, 95% CI of 0.643 to 0.887. Both CRP and WBC count showed higher discriminatory values between complicated and uncomplicated appendicitis, p < 0.001. Conclusion Alvarado score outperformed other tests in setting the diagnosis of acute appendicitis. However, none of the tests can be relied on wholly for operative decision. Clinical judgement remains the bedrock for diagnosis and operative management.

scholarly journals Effects of Ethanolic Extract of Metracaria chamomela on Some Physiological Parameters in Male Rabbits

2015 ◽  
Vol 39 (2) ◽  
pp. 26-31
Author(s):  
Alia Hussein Ali
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Blood Cell Count

     The aim of this study is to explain the effect of Ethanolic extract 70% of Metracaria chamomella on some physiological parameters in male rabbits. Twelve adult male rabbits were used in this study and were divided equally into two groups: First group was control (C) and received normal saline for four weeks, the second group (Treated group) was intubated orally with ethanolic extract of Metracaria chamomella in a dose 70 mg/kg B.W. for four weeks. Blood samples were collected by heart puncture from each animal at the end of experiment. Blood sample was divided into a part for hematological study and a part for biochemical analysis. The value of serum urea, and creatinine were reduced in animals that received Metracaria chamomella extract at dose of 70 mg/ kg B.W. as compared with the control group. Significant decrease in serum activity of aspartate aminotransferase alanine aminotransferase and alkaline phosphatase activity was observed in Metracaria treated animal as compared with the control group. This study explained that there was significant increase in serum total protein, serum albumin and serum globulin in treated animals as compared with the control group. The value of total cholesterol, serum triacylglycerol, serum low density lipoprotein cholesterol and very low density lipoprotein cholesterol concentration were reduced significantly in animal received Metracaria chamomella extract while the high density lipoprotein cholesterol was elevated significantly as compared with control group. While the effect of Metracaria chamomella extract on blood picture showed no changes in red blood cell count, hemoglobin concentration and hematocrit value but showed significant decrease in platelet count, and significant   increase in white blood cell count as compared with control group. Over all this study explained that Metracaria chamomella extract had Reno protective and hypolipidimic effect in male rabbit.  

scholarly journals Effects of Aqueous Extract of Basella alba L. leaves on Blood Cell Count in Rats

2019 ◽  
Vol 22 (1) ◽  
pp. 73-78
Author(s):  
Farhana Sabrin ◽  
AF Mohammed Shafiqul Alam ◽  
Muhammad Rashedul Islam ◽  
Md Elias Al Mamun ◽  
Jakir Ahmed Chowdhury
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Aqueous Extract ◽  
Control Group ◽  
P Value ◽  
Blood Cell Count ◽  
Basella Alba ◽  
Group A ◽  
Group B ◽  
Group D

The effect of aqueous extract of Basella alba (puishak) leaves on blood cell count of rats was studied. Forty rats of both sexes weighing between 100-150 gm were used. The rats were divided into four groups (7 rats in each group), with group A as the control group and experimental groups were denoted as B, C and D. With all aseptic precautions, aqueous extract of Basella alba (Puishak) leaves was administered into the three different doses (For group B: 60 mg/kg bw, group C: 80 mg/kg bw and group D: 100 mg/kg bw). Control group A also received distilled water as a placebo at the dose of 10 mg/kg bw for 14 days. At day 15, blood samples were collected and sent for haematological analysis. Data analysis of blood count profile of 28 rats revealed that there is an increased number of RBC, WBC and platelet count in experimental groups than in control group. ANOVA test revealed that increased blood cell counts following administration of aqueous leaves extract of keeves of B. alba were statistically significant (p value for each case was .0001<.05) than control group. Paired samples t test was performed to compare between baseline hematological parameters and parameters after 14 days of intervention. Then comparison between Group A & Group B, between Group A & Group C and between Group A & Group D were done. In all cases, probability (p) value < 0.05 was considered as statistically significant.This it is clearly evident that aqueous extract of B.alba has positive stimulant effect on blood cells count of rats. Moreover, it was found that increment of doses also increases the cell count that is positively correlated with the hypothesis. Bangladesh Pharmaceutical Journal 22(1): 73-78, 2019

scholarly journals Changes in the peripheral blood cell count in pediatric patients with Down syndrome

2019 ◽  
Vol 47 (8) ◽  
pp. 3757-3762
Author(s):  
Niculina Mang ◽  
Anda C. Vizitiu ◽  
Andrei Anghel
Keyword(s):  
Down Syndrome ◽  
Blood Cell ◽  
Cell Count ◽  
Peripheral Blood ◽  
Thyroid Dysfunction ◽  
Blood Count ◽  
Pediatric Patients ◽  
Complete Blood Count ◽  
Peripheral Blood Cell ◽  
Blood Cell Count

Objectives Down syndrome (DS) is associated with multiple complications, including a high risk of leukemia and thyroid dysfunction. This clinical study aimed to examine the complete blood cell count in patients with DS without leukemia or transient abnormal myelopoiesis. We also aimed to evaluate the effect of thyroid dysfunction on hematological anomalies in DS. Methods We analyzed the peripheral blood cell count in 23 pediatric patients with DS with and without thyroid dysfunction and in 17 pediatric patients without DS with thyroid dysfunction. Results Patients with DS showed greater neutrophilia and lymphopenia than did patients with DS and hypothyroidism and patients with hypothyroidism. Surprisingly, patients with DS showed a significant degree of eosinopenia in the peripheral blood. Interestingly, hypothyroidism had an attenuating effect on different lineages in the complete blood count. However, these anomalies were specific for DS. Conclusions Our clinical findings support previous data on DS-associated changes in the complete blood count. Our study also shows novel alterations in the complete blood count in leukemia-free patients with DS in association with hypothyroidism. The attenuating effect of thyroid dysfunction on changes in different lineages in the context of DS is novel and deserves further analysis in larger studies.

3331Impact of baseline hemoglobin level and white blood cell count in real-world patients undergoing contemporary percutaneous coronary intervention: insights from the GLOBAL LEADER study

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
P Chichareon ◽  
R Modolo ◽  
M Tomaniak ◽  
N Kogame ◽  
G Fontos ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Blood Cell ◽  
Cell Count ◽  
Cox Model ◽  
Hemoglobin Level ◽  
P Value ◽  
Blood Cell Count ◽  
Global Leader ◽  
All Cause Mortality ◽  
Wbc Count

Abstract Introduction The impact of hemoglobin (Hb) level and white blood cell count (WBC) on the outcomes in all-comers PCI patients is unknown. Purpose We sought to assess the association between baseline Hb level, WBC count on 2-year outcomes after PCI in all-comers patients in the GLOBAL LEADERS study. We compared the outcomes between anemic and non-anemic patients according to WHO definition. Methods GLOBAL LEADERS study assessed the efficacy and safety of two antiplatelet strategies in 15,991 patients undergoing PCI. The primary endpoint was all-cause mortality or new Q wave myocardial infarction (MI) at 2 years. Secondary safety endpoint was BARC 3 or 5 bleeding at 2 years. The association between WBC count, Hb level and outcomes at 2 years were assessed in the multivariable Cox model adjusted for age, diabetes, ejection fraction and renal impairment. For Hb level, patients were categorized according to the WHO definition of anemia (Hb <12 g/dL in women, Hb <13 g/dL in men). Results Of 15991 patients randomized in the GLOBAL LEADER study, baseline WBC count and Hb levels were available in 14960 (93.7%) patients and 15215 (95.3%) patients, respectively. Hb level had an inverse association with adverse events after PCI. In the multivariable Cox model, Hb level was an independent predictor for ischemic and bleeding outcomes at 2 years while the WBC count was not (see table). Compared with non-anemic patients, anemic patients had significantly higher risk of primary endpoint (adjusted HR 2.07, 95% CI 1.72–2.49), BARC 3 or 5 bleeding (adjusted HR 1.49 95% CI 1.14–1.96), all-cause mortality (adjusted HR 2.33, 95% CI 1.89–2.86), any MI (adjusted HR 1.41, 95% CI 1.11–1.80), and any revascularization (adjusted HR 1.20, 95% CI 1.03–1.39). Hb level, WBC count and 2-year outcomes Outcomes at 2 years Hemoglobin level (mg/dL) WBC count (109/L) HR (95% CI) P value HR (95% CI) P value All-cause mortality or new Q wave MI 0.87 (0.82–0.91) <0.0001 1.00 (0.999–1.002) 0.33 All-cause mortality 0.82 (0.78–0.87) <0.0001 1.00 (0.999–1.002) 0.37 Any myocardial infarction 0.93 (0.87–0.99) 0.0165 1.00 (0.996–1.001) 0.23 Any revascularization 0.96 (0.93–1.00) 0.0302 1.00 (1.00–1.001) 0.25 BARC 3 or 5 bleeding 0.85 (0.79–0.91) <0.0001 1.00 (0.997–1.002) 0.76 Conclusion In the all-comers patients undergoing PCI, the baseline Hb level was significantly associated with the ischemic and bleeding outcomes at 2 years whereas baseline WBC count was not. Baseline WBC count may not be useful as a prognostic factor after PCI.

scholarly journals Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

2007 ◽  
Vol 50 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Nilton Massuo Ishikawa ◽  
Maria José Tavares Ranzani-Paiva ◽  
Julio Vicente Lombardi ◽  
Cláudia Maris Ferreira
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Oreochromis Niloticus ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Set Up

Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

scholarly journals Retrospective study of white blood cell count and hematocrit in dengue fever

2018 ◽  
Vol 5 (1) ◽  
pp. 24 ◽  
Author(s):  
Denesh Narasimhan ◽  
Silpita Katragadda ◽  
M. Sathish
Keyword(s):  
Retrospective Study ◽  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell Count ◽  
White Blood Cell ◽  
Dengue Infection ◽  
Significant Proportion ◽  
Blood Cell Count ◽  
Baseline Levels ◽  
Wbc Count

Background: Dengue is an extremely important tropical disease transmitted by the Aedes aegypti mosquito. Aim of the study: to study the white blood cell levels in serologically proven dengue cases; to study hematocrit levels in cases of dengue fever.Methods: The present study is a retrospective observational analysis of white blood cell count (WBC count) and hematocrit in 235 serologically proven Dengue cases admitted in the years 2011-2012Results: 107 cases (45.5%) had leukopenia, 111 cases (47.2%) had normal WBC counts and 12 cases had leukocytosis (5.1%).  23 cases had increased hematocrit (9.8%), 76 cases had a low hematocrit (32.3%) and 128 cases had a normal hematocrit (54.5%).Conclusions: A significant proportion of patients had leukopenia which may be an early marker of dengue infection. Only a small percentage of patients had raised hematocrit. It may be that rise from baseline levels of haematocrit which may be more significant than absolute values.

scholarly journals Importance of haemogram parameters for prediction of the time of birth in women diagnosed with threatened preterm labour

2020 ◽  
Vol 48 (4) ◽  
pp. 030006052091843
Author(s):  
Harun Egemen Tolunay ◽  
Erkan Elci
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Blood Count ◽  
Complete Blood Count ◽  
Preterm Labour ◽  
Blood Cell Count ◽  
Group 2 ◽  
Lymphocyte Cell ◽  
Neutrophil Cell ◽  
Group 1

Objective This study aimed to estimate the importance of complete blood count parameters for predicting the timing of birth in threatened preterm labour cases. Methods We performed a retrospective study of 92 patients who were diagnosed with threatened preterm labour (24–34 gestational weeks). The patients were divided into two groups according to the time of birth (group 1: delivered within the first week after diagnosis; group 2: delivered later than 1 week). We compared characteristics and complete blood count parameters between these two groups. Results There were no significant differences in maternal age, body mass index, gravida, parity, haemoglobin levels, and gestational weeks between the two groups. The mean cervical length was 24.24 ± 3.60 mm in group 1 and 30.70 ± 5.32 mm in group 2. There were significant differences in the neutrophil to lymphocyte ratio, white blood cell count, red cell distribution width (RDW), absolute lymphocyte cell count, and absolute neutrophil cell count between the two groups. Conclusion Maternal serum RDW, the neutrophil to lymphocyte ratio, white blood cell count, absolute lymphocyte cell count, and the absolute neutrophil cell count profile could guide clinicians in predicting the time of birth in threatened preterm labour cases.

Daily Trends in White Blood Cell Count and Temperature after Subarachnoid Hemorrhage from Aneurysm

Neurosurgery ◽  
1989 ◽  
Vol 25 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Bryce Weir ◽  
Lew Disney ◽  
Michael Grace ◽  
Paul Roberts
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Blood Cell ◽  
Cell Count ◽  
Retrospective Analysis ◽  
White Blood Cell Count ◽  
White Blood Cell ◽  
Blood Cell Count ◽  
Clinically Significant ◽  
Lower Temperature ◽  
Wbc Count

Abstract A retrospective analysis of the cases of 173 patients operated on for aneurysms and admitted to a neurosurgical service early after subarachnoid hemorrhage was conducted with respect to white blood cell (WBC) count and highest daily temperature. Daily trends for the development of clinically significant vasospasm (VSP) as well as mortality during the hospitalization were analyzed. An admission WBC count greater than 15 × 109/I was associated with 55% mortality as opposed to 25% mortality for those with a lower WBC count. The mortality of those with a temperature greater than 37.5°C on Day 0 was 60%, compared with 35% for those with a lower temperature. A WBC count greater than 15 × 109/1 on Day 0 was associated with a VSP rate of 40%; a lower WBC count was associated with a VSP rate of 30%. Day 0 temperatures &gt;37.5°C were associated with a VSP rate of 40%, while patients with lower temperature had a VSP rate of 30%. By Day 6, the patients with temperatures &gt;37.5°C had a VSP rate of 60%, double that of the VSP rate of those with temperatures &lt;37.5°C. WBC count was apparently more closely linked to the chance of dying than the chance of developing VSP. The development of fever after a few days is related to both increased mortality and increased chance of developing VSP.

Sign in / Sign up

Export Citation Format

Share Document